LOCUS       V007998                 8653 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V007998
VERSION     V007998
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8653)
  AUTHORS   Olson DG.
  TITLE     Conversion of phosphoenolpyruvate to pyruvate in Clostridium
            thermocellum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8653)
  AUTHORS   Olson DG.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2016) Thayer School of Engineering, Dartmouth
            College, 14 Engineering Drive, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 8653)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8653)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (08-FEB-2016) Thayer School of Engineering, Dartmouth College, 14
            Engineering Drive, Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..455)
                     /direction=LEFT
                     /label="C. thermocellum replication origin"
                     /note="C. thermocellum replication origin"
     CDS             complement(561..1418)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1419..1523)
                     /label="AmpR promoter"
     CDS             3279..3926
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             3947..4492
                     /codon_start=1
                     /gene="hpt"
                     /product="hypoxanthine phosphoribosyltransferase"
                     /label="hpt"
                     /note="Hpt; counterselection with 8AZH"
                     /protein_id="AOC59197.1"
                     /translation="MENLSKDIDEILITEEELKEKIKELGRQITKDYKGKNLMLVGVLK
                     GALMFMADLSRHIDLPLSLDFMAVSSYGSSTHSSGIVKIIKDLDISIEGKDVLIVEDII
                     DSGLTLSYLRETLLGRKPKSLKICTILDKPERREASVKVDYVGFKIPDKFVVGYGLDFD
                     EKYRNLPFIGVLKPEMYS"
     gene            3947..4492
                     /gene="hpt"
                     /label="hpt"
     terminator      5251..5439
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     rep_origin      complement(5699..6287)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6374..6952)
                     /codon_start=1
                     /gene="tdk"
                     /product="thymidine kinase"
                     /label="tdk"
                     /note="Tdk; counterselection with FUDR"
                     /protein_id="AOC59194.1"
                     /translation="MYGPKDHGYIEVVTGPMFSGKSEELIRRIKRAKIARQKVQVFKPA
                     IDDRYSIDKVVSHNGDNMHAIAIVKASDILAYAEEDTDVFAIDEVQFFDSEIVDIVKEI
                     ADSGKRVICAGLDMDFRGEPFGPTPELMAIAEFVDKLTAICMKCGNPATRTQRLINGKP
                     ANYDDPIIMVGAKESYEARCRKCHEVPRT"
     gene            complement(6374..6952)
                     /gene="tdk"
                     /label="tdk"
     CDS             complement(7652..8653)
                     /label="repB"
                     /note="RepB replication protein"
ORIGIN
        1 ctatttagtt atttgtttgg tcacttataa ctttaacaga tggggttttt ctgtgcaacc
       61 aattttaagg gttttcaata ctttaaaaca catacatacc aacacttcaa cgcacctttc
      121 agcaactaaa ataaaaatga cgttatttct atatgtatca agaatagaaa gaactcgttt
      181 ttcgctacgc tcaaaacgca aaaaaagcac tcattcgagt gctttttctt atcgctccaa
      241 atcatgcgat tttttcctct ttgcttttct ttgctcacga agttctcgat cacgctgcaa
      301 aacatcttga agcgaaaaag tattcttctt ttcttccgat cgctcatgct gacgcacgaa
      361 aagccctcta ggcgcatagg aacaactcct aaatgcatgt gaggggtttt ctcgtccatg
      421 tgaacagtcg catacgcaat attttgtttc ccatagcggc cgcgattatc aaaaaggatc
      481 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
      541 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
      601 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
      661 cgcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
      721 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
      781 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
      841 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
      901 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
      961 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     1021 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     1081 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     1141 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     1201 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     1261 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     1321 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     1381 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     1441 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     1501 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     1561 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcg atacttcctt
     1621 cgttaaaata gcttttaaaa tattttgcag aaaggaatat ctcggcaata tttttttcca
     1681 tgttaatata cgttattatg ccgtgaaccc atttttcatc gttatttatt gaaactctgc
     1741 agaaaattgg agaatcattt tgaggaagca aatcgtttaa caatttaacc accctgcctt
     1801 ttacaattat tcatatcata aattttatcg tcaaaaacgt taaaattcta gacaaaaaac
     1861 cgacatatat tttgttgatt ttataaagat ttatataaaa gcattaaaag gctgtggtcc
     1921 tttttttatt tttacatcat aaacatttgg ggaaaatatt gcaatatgaa ataaagtagg
     1981 atataattga taataattat aaccaggtaa taatatcata taataaataa aaagtaacag
     2041 atataaataa aagttgcaat tgtattactc aaaatgtata cgactgcatt tggaggctac
     2101 tggatacaaa accaataaat aaatgacaag aggtgggaaa ctgatccaat acaggaaagc
     2161 tcaaaaatat aagatagaac cggacatggt cgattacgaa aacagggttc atccggttta
     2221 aagtagagta ttgagccatt tgtctcagaa atacaataaa agcttgaatt tatgattcaa
     2281 aataaaaaac tcaggacctg acatggtgcc tgagtttttt gctgtattca aattcattaa
     2341 aactaatact tttttttatt aaataacaat agacaaaaat aaattgaata tgataaaata
     2401 tggattgaaa atcaattatt aaaaataaaa tagttatatg taaaaacaat atatttttct
     2461 atgaggtatt cgttatgaga aaaatatgtt gcttgttgtt tgctgtattc attctttgta
     2521 tttcagcgtc atgctatcaa agaaaagaaa cacgaataag ccaacctgtt ataatgcctg
     2581 ccatccaaag ccctgaagat attgatgatt acatagggga aaagggatat gagattttag
     2641 agtctaaagg aaagactgag gaatacacat tagacaaaaa aagggtgtat aacaattggc
     2701 atcgtgggaa taggcatgga aaagattttt ttgcccgagg atgcattgat aaagtatttc
     2761 ttttccgaaa gagaggaaaa gattctaaag agttttggaa atactgatga atattgtgtg
     2821 cagagtacaa ttctatggac aagaaaagag gctttgtcaa aactttttcg tctgggaatg
     2881 aggatggatt ttaaaaagct ggatactttg gagggcgagg tggtttttca ggaaacaaac
     2941 agggcgcgtc tgttttcttt tatatgcaat aattactgta tctctctggc attgccaggt
     3001 tttaataaag attaaaatta ttgactagaa ataaaaaaat tgtccataat attaatggac
     3061 aaaaaaacaa agaattacat caaaggaaga taaaaatact ttgttaaaaa attaattatt
     3121 ttttatctaa actattgaaa atgaaaataa aataatataa aatgaatcat agtgcaagag
     3181 atacttgcca gaggatgaat attttactgc attcatgctt tatggcagct aatagaggca
     3241 ttaaattaaa ttttaattta caataggagg cgatattaat gaactttaat aaaattgatt
     3301 tagacaattg gaagagaaaa gagatattta atcattattt gaaccaacaa acgactttta
     3361 gtataaccac agaaattgat attagtgttt tataccgaaa cataaaacaa gaaggatata
     3421 aattttaccc tgcatttatt ttcttagtga caagggtgat aaactcaaat acagctttta
     3481 gaactggtta caatagcgac ggagagttag gttattggga taagttagag ccactttata
     3541 caatttttga tggtgtatct aaaacattct ctggtatttg gactcctgta aagaatgact
     3601 tcaaagagtt ttatgattta tacctttctg atgtagagaa atataatggt tcggggaaat
     3661 tgtttcccaa aacacctata cctgaaaatg ctttttctct ttctattatt ccatggactt
     3721 catttactgg gtttaactta aatatcaata ataatagtaa ttaccttcta cccattatta
     3781 cagcaggaaa attcattaat aaaggtaatt caatatattt accgctatct ttacaggtac
     3841 atcattctgt ttgtgatggt tatcatgcag gattgtttat gaactctatt caggaattgt
     3901 cagataggcc taatgactgg cttttataat aaaggaggtc gacgtcatgg aaaatttatc
     3961 aaaagacatc gatgaaattt tgatcacaga agaagaactt aaggaaaaga taaaagagct
     4021 tgggaggcaa atcacaaaag actacaaagg gaaaaatttg atgttggtag gagttttaaa
     4081 aggtgcttta atgtttatgg ctgatttgtc aagacacata gatttgcctt tatcacttga
     4141 ttttatggct gtttccagct atggaagctc aactcattca tcaggaatag taaagataat
     4201 caaagatctt gatataagca tagaaggcaa agatgttctg attgtggaag acataattga
     4261 cagcggtttg actttgtctt acttaaggga aactttactt ggaaggaagc caaaaagcct
     4321 gaaaatatgc acaatattag acaaaccgga gagaagagaa gcatctgtaa aagtcgatta
     4381 tgtaggattt aagatacctg ataagtttgt cgtgggttat ggattggact ttgatgaaaa
     4441 gtacaggaac cttcctttta taggcgtttt gaaacctgaa atgtacagct aagcccgggc
     4501 ctcgagaaaa caaaaggctc agtcggaaga ctgggccttt tgttttggta ccgaattcgg
     4561 cgcgcctcag cgtttaaacc cgctgatcct agagggccgc atcatgtaat tagttatgtc
     4621 acgcttacat tcacgccctc cccatgcttt aatacatacc tggcccagta gttcagaaat
     4681 gcatgccgca aaaatcacca aagtaatgga catggcaatg aaaatgggcg caccgtttat
     4741 aagcattaat gattccggcg gtgcaagaat tgaagaagga attgacgcac tcaagggatt
     4801 tggagatatc ttctacagaa atactttggc ttcaggtgta attccccaga tttcagttat
     4861 catgggacca tgcgcaggcg gagcggtata ttctcctgca ataaccgact ttatatttat
     4921 ggttgacaaa accagtcaga tgtttataac gggaccccag gtaattaagt ccgtaaccgg
     4981 agaagacgtg acttttgaaa aacttggcgg tgcggaaacc cacaactcca taagcggtgt
     5041 tgctcacttc agaagttcaa gtgaaaaaga atgtatagag caaatcaaaa agcttattag
     5101 ttatcttcct gataacaatc tttccgatgt tccgattgtt ccaactcagg atgacataaa
     5161 cagaattact gacaacctgg tcgatatcat tccgcaggac tccaacaagc cttatgacct
     5221 gctaatagta gtgaaaaaat cagggataat gccgctctaa ccgaaaagga aggagttaga
     5281 caacctgaag tctaggtccc tatttatttt tttatagtta tgttagtatt aagaacgtta
     5341 tttatatttc aaatttttct tttttttctg tacagacgcg tgtacgcatg taacattata
     5401 ctgaaaacct tgcttgagaa ggttttggga cgctcgaagg ctttaatttg caagctgcgg
     5461 ccctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     5521 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     5581 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     5641 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     5701 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     5761 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     5821 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     5881 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     5941 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     6001 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     6061 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     6121 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     6181 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     6241 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     6301 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     6361 tgaaaaaatc cgcttaagtc cgcgggactt catggcactt tctacacctt gcttcataag
     6421 actcctttgc tccaaccatt ataatgggat cgtcgtaatt ggcaggcttc ccatttataa
     6481 gcctttgcgt gcgagtagca ggattgccac acttcatgca tatagcagta agcttatcga
     6541 caaattcagc tatggccatc aattctggag tcggaccaaa tggttcacct ctaaagtcca
     6601 tgtcaagccc tgcacatatg actcttttcc cgctatcggc aatctctttt acgatgtcga
     6661 ctatttcaga atcaaaaaat tgaacttcat ctatagcaaa tacatccgta tcttcttcag
     6721 cataagccaa tatgtcagaa gcctttacta tggcaatggc gtgcatgttg tcgccgttat
     6781 gagatacgac tttgtctatg gaatacctat cgtctatagc cggtttgaaa acctgaactt
     6841 tttgcctggc aatcttagct ctctttatcc ttcttataag ttcctcactt tttccactga
     6901 acatgggacc agttacaact tctatgtacc cgtggtcttt aggcccatac atctttcgtc
     6961 ctccttaaaa ttttcgttta tttgtttttt atttagtgtg cctaaaagca ccatcttata
     7021 cattcgtacc ttatatctct tatcttgtat ctcttacctc atatctctta ttattaacta
     7081 ccgaagtata ttttcataac ggaatcttat gacttatttt tctgactgcc gttttaccat
     7141 acggttaatt tatacggaaa cagctaatct aaccttgctg aaggcatgtt taccaaaata
     7201 ttatatctac tttaccatat taattcaagt ggtcattttg atattttacg ccaaaatttt
     7261 tagcgttcct ttacttgata atttatccaa aaaccttcat tcgtcaaaac aattatgata
     7321 taattaattt atgatatgat taattaatat ttacggataa ctaatcattt caatgcaata
     7381 gatcaacatg caaatcaata tccaccttac tccgaacatt ttgcaccaac gaatgcccag
     7441 cactatatcc cggcaagcaa tataacgtct ataatttttt atgataacga taaaattaaa
     7501 ttttatgaaa ggggtaaaag catgagaaaa atagctgtat tgttaattac tttgacgttc
     7561 ttagtcacga ctcgaattta ggaggcttac ttgtctgctt tcttcattag aatcaatcct
     7621 tttttaaaag tcaatcccgt ttgttgaact actctttaat aaaataattt ttccgttccc
     7681 aattccacat tgcaataata gaaaatccat cttcatcggc tttttcgtca tcatctgtat
     7741 gaatcaaatc gccttcttct gtgtcatcaa ggtttaattt tttatgtatt tcttttaaca
     7801 aaccaccata ggagattaac cttttacggt gtaaaccttc ctccaaatca gacaaacgtt
     7861 tcaaattctt ttcttcatca tcggtcataa aatccgtatc ctttacagga tattttgcag
     7921 tttcgtcaat tgccgattgt atatccgatt tatatttatt tttcggtcga atcatttgaa
     7981 cttttacatt tggatcatag tctaatttca ttgccttttt ccaaaattga atccattgtt
     8041 tttgattcac gtagttttct gtattcttaa aataagttgg ttccacacat accaatacat
     8101 gcatgtgctg attataagaa ttatctttat tatttattgt cacttccgtt gcacgcataa
     8161 aaccaacaag atttttatta atttttttat attgcatcat tcggcgaaat ccttgagcca
     8221 tatctgacaa actcttattt aattcttcgc catcataaac atttttaact gttaatgtga
     8281 gaaacaacca acgaactgtt ggcttttgtt taataacttc agcaacaacc ttttgtgact
     8341 gaatgccatg tttcattgct ctcctccagt tgcacattgg acaaagcctg gatttacaaa
     8401 accacactcg atacaacttt ctttcgcctg tttcacgatt ttgtttatac tctaatattt
     8461 cagcacaatc ttttactctt tcagcctttt taaattcaag aatatgcaga agttcaaagt
     8521 aatcaacatt agcgattttc ttttctctcc atggtctcac ttttccactt tttgtcttgt
     8581 ccactaaaac ccttgatttt tcatctgaat aaatgctact attaggacac ataatattaa
     8641 aagaaacccc cat
//
