LOCUS       V008000                 4475 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V008000
VERSION     V008000
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Hon S, Lanahan AA, Tian L, Giannone RJ, Hettich RL, Olson DG, Lynd
            LR.
  TITLE     Development of a plasmid-based expression system in Clostridium
            thermocellum and its use to screen heterologous expression of
            bifunctional alcohol dehydrogenases (adhEs)
  JOURNAL   Metab Eng Commun 3, 120-129 (2016)
REFERENCE   2  (bases 1 to 4475)
  AUTHORS   Hon S, Lanahan AA, Tian L, Giannone RJ, Hettich RL, Olson DG, Lynd
            LR.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2016) Thayer School of Engineering, Dartmouth
            College, 14 Engineering Drive, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 4475)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4475)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng
            Commun 3, 120-129 (2016)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-MAY-2016) Thayer School of Engineering, Dartmouth College, 14
            Engineering Drive, Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4475
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..1002
                     /label="repB"
                     /note="RepB replication protein"
     misc_feature    1011..1051
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     regulatory      1088..1179
                     /gene="cat"
                     /regulatory_class="promoter"
     CDS             1180..1827
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(1911..2768)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2769..2860)
                     /label="AmpR promoter"
     rep_origin      3460..4005
                     /direction=RIGHT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
ORIGIN
        1 atgggggttt cttttaatat tatgtgtcct aatagtagca tttattcaga tgaaaaatca
       61 agggttttag tggacaagac aaaaagtgga aaagtgagac catggagaga aaagaaaatc
      121 gctaatgttg attactttga acttctgcat attcttgaat ttaaaaaggc tgaaagagta
      181 aaagattgtg ctgaaatatt agagtataaa caaaatcgtg aaacaggcga aagaaagttg
      241 tatcgagtgt ggttttgtaa atccaggctt tgtccaatgt gcaactggag gagagcaatg
      301 aaacatggca ttcagtcaca aaaggttgtt gctgaagtta ttaaacaaaa gccaacagtt
      361 cgttggttgt ttctcacatt aacagttaaa aatgtttatg atggcgaaga attaaataag
      421 agtttgtcag atatggctca aggatttcgc cgaatgatgc aatataaaaa aattaataaa
      481 aatcttgttg gttttatgcg tgcaacggaa gtgacaataa ataataaaga taattcttat
      541 aatcagcaca tgcatgtatt ggtatgtgtg gaaccaactt attttaagaa tacagaaaac
      601 tacgtgaatc aaaaacaatg gattcaattt tggaaaaagg caatgaaatt agactatgat
      661 ccaaatgtaa aagttcaaat gattcgaccg aaaaataaat ataaatcgga tatacaatcg
      721 gcaattgacg aaactgcaaa atatcctgta aaggatacgg attttatgac cgatgatgaa
      781 gaaaagaatt tgaaacgttt gtctgatttg gaggaaggtt tacaccgtaa aaggttaatc
      841 tcctatggtg gtttgttaaa agaaatacat aaaaaattaa accttgatga cacagaagaa
      901 ggcgatttga ttcatacaga tgatgacgaa aaagccgatg aagatggatt ttctattatt
      961 gcaatgtgga attgggaacg gaaaaattat tttattaaag agtagttcaa cccggggatc
     1021 ctctagagtc gacctgcagg catgcaagct ttaatagaaa taattttgtt taactttaca
     1081 aacgggattg acttttaaaa aaggattgat tctaatgaag aaagcagaca agtaagcctc
     1141 ctaaattcac tttagataaa aatttaggag gcatatcaaa tgaactttaa taaaattgat
     1201 ttagacaatt ggaagagaaa agagatattt aatcattatt tgaaccaaca aacgactttt
     1261 agtataacca cagaaattga tattagtgtt ttataccgaa acataaaaca agaaggatat
     1321 aaattttacc ctgcatttat tttcttagtg acaagggtga taaactcaaa tacagctttt
     1381 agaactggtt acaatagcga cggagagtta ggttattggg ataagttaga gccactttat
     1441 acaatttttg atggtgtatc taaaacattc tctggtattt ggactcctgt aaagaatgac
     1501 ttcaaagagt tttatgattt atacctttct gatgtagaga aatataatgg ttcggggaaa
     1561 ttgtttccca aaacacctat acctgaaaat gctttttctc tttctattat tccatggact
     1621 tcatttactg ggtttaactt aaatatcaat aataatagta attaccttct acccattatt
     1681 acagcaggaa aattcattaa taaaggtaat tcaatatatt taccgctatc tttacaggta
     1741 catcattctg tttgtgatgg ttatcatgca ggattgttta tgaactctat tcaggaattg
     1801 tcagataggc ctaatgactg gcttttataa tatgagataa tgccgactgt actttttaca
     1861 gtcggttttc taatgtcact agggctcgcc tttgggaagt ttgaaggtta ccaatgctta
     1921 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     1981 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     2041 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     2101 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     2161 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     2221 gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     2281 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     2341 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     2401 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     2461 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     2521 tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     2581 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     2641 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     2701 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     2761 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     2821 agcggataca tatttgaatg tatttagaaa aataaacaaa agagtttgag gaaaacttaa
     2881 gaaaacggga aactatttcc tgttgagaat gtgaaattaa atgataaaaa gtgatataat
     2941 agggtcaggc gtacagaagc ctgacctttt tgcaaaattg ttttattggc gcggcgtata
     3001 tatacagcat atatacaatg taaatttata caatcaaatt gttgatacaa tcgaattatg
     3061 cggctaaaaa tttgctcgcg attgctgaat aaaagatacg agagacctct cttgtatctt
     3121 ttttattttg agtggttttg tccgttacac tagaaaaccg aaagacaata aaaattttat
     3181 tcttgctgag tctggctttc ggtaagctag acaaaacgga caaaataaaa attggcaagg
     3241 gtttaaaggt ggagattttt tgagtgatct tctcaaaaaa tactacctgt cccttgctga
     3301 tttttaaacg agcacgagag caaaaccccc ctttgctgag gtggcagagg gcaggttttt
     3361 ttgtttcttt tttctcgtaa aaaaaagaaa ggtcttaaag gttttatggt tttggtcatg
     3421 agcctagaaa tattttatct gattaataag atgatcttct tgagatcgtt ttggtctgcg
     3481 cgtaatctct tgctctgaaa acgaaaaaac cgccttgcag ggcggttttt cgaaggttct
     3541 ctgagctacc aactctttga accgaggtaa ctggcttgga ggagcgcagt caccaaaact
     3601 tgtcctttca gtttagcctt aaccggcgca tgacttcaag actaactcct ctaaatcaat
     3661 taccagtggc tgctgccagt ggtgcttttg catgtctttc cgggttggac tcaagacgat
     3721 agttaccgga taaggcgcag cggtcggact gaacgggggg ttcgtgcata cagtccagct
     3781 tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg aatgagacaa acgcggccat
     3841 aacagcggaa tgacaccggt aaaccgaaag gcaggaacag gagagcgcac gagggagccg
     3901 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacca ctgatttgag
     3961 cgtcagattt cgtgatgctt gtcagggggg cggagcctat ggaaaaacgg ctttgccgcg
     4021 gccctctcac ttccctgtta agtatcttcc tggcatcttc caggaaatct ccgccccgtt
     4081 cgtaagccat ttccgctcgc cgcagtcgaa cgaccgagcg tagcgagtca gtgagcgagg
     4141 aagcggaata tatcctgtat cacatattct gctgacgcac cggtgcagcc ttttttctcc
     4201 tgccacatga agcacttcac tgacaccctc atcagtgcca acatagtaag ccagtataca
     4261 aatgagtgct ttttttgcgt tttgagcgta gcgaaaaacg agttctttct attcttgata
     4321 catatagaaa taacgtcatt tttattttag ttgctgaaag gtgcgttgaa gtgttggtat
     4381 gtatgtgttt taaagtattg aaaaccctta aaattggttg cacagaaaaa ccccatctgt
     4441 taaagttata agtgaccaaa caaataacta aatag
//
