LOCUS       V008001                 4135 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V008001
VERSION     V008001
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Hon S, Lanahan AA, Tian L, Giannone RJ, Hettich RL, Olson DG, Lynd
            LR.
  TITLE     Development of a plasmid-based expression system in Clostridium
            thermocellum and its use to screen heterologous expression of
            bifunctional alcohol dehydrogenases (adhEs)
  JOURNAL   Metab Eng Commun 3, 120-129 (2016)
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Hon S, Lanahan AA, Tian L, Giannone RJ, Hettich RL, Olson DG, Lynd
            LR.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2016) Thayer School of Engineering, Dartmouth
            College, 14 Engineering Drive, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 4135)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4135)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng
            Commun 3, 120-129 (2016)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-MAY-2016) Thayer School of Engineering, Dartmouth College, 14
            Engineering Drive, Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4135
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..1002
                     /label="repB"
                     /note="RepB replication protein"
     misc_feature    1011..1051
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     regulatory      1088..1179
                     /gene="cat"
                     /regulatory_class="promoter"
     CDS             1180..1827
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(1911..2768)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2769..2860)
                     /label="AmpR promoter"
     rep_origin      3120..3665
                     /direction=RIGHT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
ORIGIN
        1 atgggggttt cttttaatat tatgtgtcct aatagtagca tttattcaga tgaaaaatca
       61 agggttttag tggacaagac aaaaagtgga aaagtgagac catggagaga aaagaaaatc
      121 gctaatgttg attactttga acttctgcat attcttgaat ttaaaaaggc tgaaagagta
      181 aaagattgtg ctgaaatatt agagtataaa caaaatcgtg aaacaggcga aagaaagttg
      241 tatcgagtgt ggttttgtaa atccaggctt tgtccaatgt gcaactggag gagagcaatg
      301 aaacatggca ttcagtcaca aaaggttgtt gctgaagtta ttaaacaaaa gccaacagtt
      361 cgttggttgt ttctcacatt aacagttaaa aatgtttatg atggcgaaga attaaataag
      421 agtttgtcag atatggctca aggatttcgc cgaatgatgc aatataaaaa aattaataaa
      481 aatcttgttg gttttatgcg tgcaacggaa gtgacaataa ataataaaga taattcttat
      541 aatcagcaca tgcatgtatt ggtatgtgtg gaaccaactt attttaagaa tacagaaaac
      601 tacgtgaatc aaaaacaatg gattcaattt tggaaaaagg caatgaaatt agactatgat
      661 ccaaatgtaa aagttcaaat gattcgaccg aaaaataaat ataaatcgga tatacaatcg
      721 gcaattgacg aaactgcaaa atatcctgta aaggatacgg attttatgac cgatgatgaa
      781 gaaaagaatt tgaaacgttt gtctgatttg gaggaaggtt tacaccgtaa aaggttaatc
      841 tcctatggtg gtttgttaaa agaaatacat aaaaaattaa accttgatga cacagaagaa
      901 ggcgatttga ttcatacaga tgatgacgaa aaagccgatg aagatggatt ttctattatt
      961 gcaatgtgga attgggaacg gaaaaattat tttattaaag agtagttcaa cccggggatc
     1021 ctctagagtc gacctgcagg catgcaagct ttaatagaaa taattttgtt taactttaca
     1081 aacgggattg acttttaaaa aaggattgat tctaatgaag aaagcagaca agtaagcctc
     1141 ctaaattcac tttagataaa aatttaggag gcatatcaaa tgaactttaa taaaattgat
     1201 ttagacaatt ggaagagaaa agagatattt aatcattatt tgaaccaaca aacgactttt
     1261 agtataacca cagaaattga tattagtgtt ttataccgaa acataaaaca agaaggatat
     1321 aaattttacc ctgcatttat tttcttagtg acaagggtga taaactcaaa tacagctttt
     1381 agaactggtt acaatagcga cggagagtta ggttattggg ataagttaga gccactttat
     1441 acaatttttg atggtgtatc taaaacattc tctggtattt ggactcctgt aaagaatgac
     1501 ttcaaagagt tttatgattt atacctttct gatgtagaga aatataatgg ttcggggaaa
     1561 ttgtttccca aaacacctat acctgaaaat gctttttctc tttctattat tccatggact
     1621 tcatttactg ggtttaactt aaatatcaat aataatagta attaccttct acccattatt
     1681 acagcaggaa aattcattaa taaaggtaat tcaatatatt taccgctatc tttacaggta
     1741 catcattctg tttgtgatgg ttatcatgca ggattgttta tgaactctat tcaggaattg
     1801 tcagataggc ctaatgactg gcttttataa tatgagataa tgccgactgt actttttaca
     1861 gtcggttttc taatgtcact agggctcgcc tttgggaagt ttgaaggtta ccaatgctta
     1921 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     1981 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     2041 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     2101 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     2161 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     2221 gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     2281 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     2341 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     2401 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     2461 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     2521 tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     2581 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     2641 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     2701 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     2761 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     2821 agcggataca tatttgaatg tatttagaaa aataaacaaa agagtttgag gaaaacttaa
     2881 gaaaacggga aactatttcc tgttgagaat gtgaaattaa atgataaaaa gtgatataat
     2941 agggtcaggc gtacagaagc ctgacctttt tgcaaaattg ttttattggc gcggcgtata
     3001 tatacagcat atatacaatg taaatttata caatcaaatt gttgatacaa tcgaattatg
     3061 cggctaaaaa tttgctcatg agcctagaaa tattttatct gattaataag atgatcttct
     3121 tgagatcgtt ttggtctgcg cgtaatctct tgctctgaaa acgaaaaaac cgccttgcag
     3181 ggcggttttt cgaaggttct ctgagctacc aactctttga accgaggtaa ctggcttgga
     3241 ggagcgcagt caccaaaact tgtcctttca gtttagcctt aaccggcgca tgacttcaag
     3301 actaactcct ctaaatcaat taccagtggc tgctgccagt ggtgcttttg catgtctttc
     3361 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcggact gaacgggggg
     3421 ttcgtgcata cagtccagct tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg
     3481 aatgagacaa acgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaacag
     3541 gagagcgcac gagggagccg ccagggggaa acgcctggta tctttatagt cctgtcgggt
     3601 ttcgccacca ctgatttgag cgtcagattt cgtgatgctt gtcagggggg cggagcctat
     3661 ggaaaaacgg ctttgccgcg gccctctcac ttccctgtta agtatcttcc tggcatcttc
     3721 caggaaatct ccgccccgtt cgtaagccat ttccgctcgc cgcagtcgaa cgaccgagcg
     3781 tagcgagtca gtgagcgagg aagcggaata tatcctgtat cacatattct gctgacgcac
     3841 cggtgcagcc ttttttctcc tgccacatga agcacttcac tgacaccctc atcagtgcca
     3901 acatagtaag ccagtataca aatgagtgct ttttttgcgt tttgagcgta gcgaaaaacg
     3961 agttctttct attcttgata catatagaaa taacgtcatt tttattttag ttgctgaaag
     4021 gtgcgttgaa gtgttggtat gtatgtgttt taaagtattg aaaaccctta aaattggttg
     4081 cacagaaaaa ccccatctgt taaagttata agtgaccaaa caaataacta aatag
//
