LOCUS       V008003                 5412 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V008003
VERSION     V008003
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5412)
  AUTHORS   Tian L, Lo J, Shao X, Zheng T, Olson DG, Lynd LR.
  TITLE     Identification of a ferredoxin:NAD+ oxidoreductase enzyme in
            Thermoanaerobacterium saccharolyticum and its role in ethanol
            formation
  JOURNAL   Appl. Environ. Microbiol. (2016) In press
   PUBMED   27694237
REFERENCE   2  (bases 1 to 5412)
  AUTHORS   Tian L.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2016) Thayer School of Engineering, Dartmouth
            College, 14 Engineering Dr, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 5412)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5412)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol. (2016) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (22-MAY-2016) Thayer School of Engineering, Dartmouth College, 14
            Engineering Dr, Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5412
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(68..925)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(926..1017)
                     /label="AmpR promoter"
     rep_origin      1617..2162
                     /direction=RIGHT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             2633..3634
                     /label="repB"
                     /note="RepB replication protein"
     regulatory      3679..3887
                     /label="P2638"
                     /note="P2638"
                     /regulatory_class="promoter"
     CDS             3888..4619
                     /codon_start=1
                     /product="putative ferredoxin:NAD+ oxidoreductase"
                     /EC_number="1.18.1.2"
                     /label="putative ferredoxin:NAD+ oxidoreductase"
                     /note="Tsac_1705"
                     /protein_id="AOU74527.1"
                     /translation="MRYVVRENREISNGIYRLRLEFDGIPTPGQFIMINCGGNTLLKRP
                     FSICDHDADEITIVYRIKGKGTYNLSKVKALDTLEVTGPHGHGFDMFDKFNNILIVGGG
                     IGIPPLLFLSKKLKSKNLKIALGFKSDVFLVDEFKKYGDVVIATEDGNTGIKGYVTDAM
                     KDEIKDIDMIYGCGPMQMLKSVKDMAEKYDVPCQISIEEHMGCGIGACMVCACKTKTDS
                     GFQYKKVCKDGPVFWSREVVF"
     CDS             4749..5396
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
ORIGIN
        1 cgactgtact ttttacagtc ggttttctaa tgtcactagg gctcgccttt gggaagtttg
       61 aaggttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
      121 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
      181 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
      241 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
      301 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
      361 gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
      421 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
      481 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
      541 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
      601 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
      661 gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag
      721 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
      781 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
      841 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
      901 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
      961 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaaaga
     1021 gtttgaggaa aacttaagaa aacgggaaac tatttcctgt tgagaatgtg aaattaaatg
     1081 ataaaaagtg atataatagg gtcaggcgta cagaagcctg acctttttgc aaaattgttt
     1141 tattggcgcg gcgtatatat acagcatata tacaatgtaa atttatacaa tcaaattgtt
     1201 gatacaatcg aattatgcgg ctaaaaattt gctcgcgatt gctgaataaa agatacgaga
     1261 gacctctctt gtatcttttt tattttgagt ggttttgtcc gttacactag aaaaccgaaa
     1321 gacaataaaa attttattct tgctgagtct ggctttcggt aagctagaca aaacggacaa
     1381 aataaaaatt ggcaagggtt taaaggtgga gattttttga gtgatcttct caaaaaatac
     1441 tacctgtccc ttgctgattt ttaaacgagc acgagagcaa aacccccctt tgctgaggtg
     1501 gcagagggca ggtttttttg tttctttttt ctcgtaaaaa aaagaaaggt cttaaaggtt
     1561 ttatggtttt ggtcatgagc ctagaaatat tttatctgat taataagatg atcttcttga
     1621 gatcgttttg gtctgcgcgt aatctcttgc tctgaaaacg aaaaaaccgc cttgcagggc
     1681 ggtttttcga aggttctctg agctaccaac tctttgaacc gaggtaactg gcttggagga
     1741 gcgcagtcac caaaacttgt cctttcagtt tagccttaac cggcgcatga cttcaagact
     1801 aactcctcta aatcaattac cagtggctgc tgccagtggt gcttttgcat gtctttccgg
     1861 gttggactca agacgatagt taccggataa ggcgcagcgg tcggactgaa cggggggttc
     1921 gtgcatacag tccagcttgg agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat
     1981 gagacaaacg cggccataac agcggaatga caccggtaaa ccgaaaggca ggaacaggag
     2041 agcgcacgag ggagccgcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2101 gccaccactg atttgagcgt cagatttcgt gatgcttgtc aggggggcgg agcctatgga
     2161 aaaacggctt tgccgcggcc ctctcacttc cctgttaagt atcttcctgg catcttccag
     2221 gaaatctccg ccccgttcgt aagccatttc cgctcgccgc agtcgaacga ccgagcgtag
     2281 cgagtcagtg agcgaggaag cggaatatat cctgtatcac atattctgct gacgcaccgg
     2341 tgcagccttt tttctcctgc cacatgaagc acttcactga caccctcatc agtgccaaca
     2401 tagtaagcca gtatacaaat gagtgctttt tttgcgtttt gagcgtagcg aaaaacgagt
     2461 tctttctatt cttgatacat atagaaataa cgtcattttt attttagttg ctgaaaggtg
     2521 cgttgaagtg ttggtatgta tgtgttttaa agtattgaaa acccttaaaa ttggttgcac
     2581 agaaaaaccc catctgttaa agttataagt gaccaaacaa ataactaaat agatgggggt
     2641 ttcttttaat attatgtgtc ctaatagtag catttattca gatgaaaaat caagggtttt
     2701 agtggacaag acaaaaagtg gaaaagtgag accatggaga gaaaagaaaa tcgctaatgt
     2761 tgattacttt gaacttctgc atattcttga atttaaaaag gctgaaagag taaaagattg
     2821 tgctgaaata ttagagtata aacaaaatcg tgaaacaggc gaaagaaagt tgtatcgagt
     2881 gtggttttgt aaatccaggc tttgtccaat gtgcaactgg aggagagcaa tgaaacatgg
     2941 cattcagtca caaaaggttg ttgctgaagt tattaaacaa aagccaacag ttcgttggtt
     3001 gtttctcaca ttaacagtta aaaatgttta tgatggcgaa gaattaaata agagtttgtc
     3061 agatatggct caaggatttc gccgaatgat gcaatataaa aaaattaata aaaatcttgt
     3121 tggttttatg cgtgcaacgg aagtgacaat aaataataaa gataattctt ataatcagca
     3181 catgcatgta ttggtatgtg tggaaccaac ttattttaag aatacagaaa actacgtgaa
     3241 tcaaaaacaa tggattcaat tttggaaaaa ggcaatgaaa ttagactatg atccaaatgt
     3301 aaaagttcaa atgattcgac cgaaaaataa atataaatcg gatatacaat cggcaattga
     3361 cgaaactgca aaatatcctg taaaggatac ggattttatg accgatgatg aagaaaagaa
     3421 tttgaaacgt ttgtctgatt tggaggaagg tttacaccgt aaaaggttaa tctcctatgg
     3481 tggtttgtta aaagaaatac ataaaaaatt aaaccttgat gacacagaag aaggcgattt
     3541 gattcataca gatgatgacg aaaaagccga tgaagatgga ttttctatta ttgcaatgtg
     3601 gaattgggaa cggaaaaatt attttattaa agagtagttc aacccgggga tcctctagag
     3661 tcgacctgca ggcatgcaga taaacaaagg acggttcagg gcttctgctc atcctactct
     3721 gcattgtaaa aaggtaggat gaatttttat ttttaatctt attgaaaaaa atttttgaaa
     3781 atcggtttta ttaaaaaaaa gtgggtatat ttataatagt caattgattg gttaaaaaaa
     3841 tttaaataag caaacagaat aataacaaaa gtaaggagga atttgttatg agatacgttg
     3901 ttagagaaaa tagagaaatt agcaatggaa tatacagact tagattagaa tttgatggga
     3961 ttcctacgcc agggcaattt atcatgataa attgcggtgg caatacatta ttaaagaggc
     4021 catttagcat atgcgatcat gatgcagatg aaatcacaat tgtttatcgg ataaaaggaa
     4081 aaggcactta taatctttcg aaggttaaag ctttagatac attagaggta actggtcctc
     4141 atggtcatgg atttgatatg tttgataaat tcaacaatat tctcatcgtt ggtggtggca
     4201 tagggatacc accactacta tttctttcaa aaaaattgaa atcgaaaaat ctaaagattg
     4261 ctcttggttt taaaagcgat gtatttcttg tggatgaatt taaaaaatat ggtgatgtcg
     4321 ttattgcgac tgaagatggg aataccggta ttaaagggta tgttaccgat gcaatgaaag
     4381 atgaaataaa ggatattgat atgatttatg gatgtggccc aatgcaaatg cttaaatctg
     4441 taaaagacat ggctgaaaag tacgatgtgc cttgtcaaat ctctattgaa gaacatatgg
     4501 gttgtggaat tggtgcgtgt atggtatgtg cctgtaaaac aaaaactgat tctggatttc
     4561 aatataaaaa agtctgtaag gatggtcctg tattttggtc aagggaggta gtattttgat
     4621 taatagaaat aattttgttt aactttacaa acgggattga cttttaaaaa aggattgatt
     4681 ctaatgaaga aagcagacaa gtaagcctcc taaattcact ttagataaaa atttaggagg
     4741 catatcaaat gaactttaat aaaattgatt tagacaattg gaagagaaaa gagatattta
     4801 atcattattt gaaccaacaa acgactttta gtataaccac agaaattgat attagtgttt
     4861 tataccgaaa cataaaacaa gaaggatata aattttaccc tgcatttatt ttcttagtga
     4921 caagggtgat aaactcaaat acagctttta gaactggtta caatagcgac ggagagttag
     4981 gttattggga taagttagag ccactttata caatttttga tggtgtatct aaaacattct
     5041 ctggtatttg gactcctgta aagaatgact tcaaagagtt ttatgattta tacctttctg
     5101 atgtagagaa atataatggt tcggggaaat tgtttcccaa aacacctata cctgaaaatg
     5161 ctttttctct ttctattatt ccatggactt catttactgg gtttaactta aatatcaata
     5221 ataatagtaa ttaccttcta cccattatta cagcaggaaa attcattaat aaaggtaatt
     5281 caatatattt accgctatct ttacaggtac atcattctgt ttgtgatggt tatcatgcag
     5341 gattgtttat gaactctatt caggaattgt cagataggcc taatgactgg cttttataat
     5401 atgagataat gc
//
