LOCUS       V008007                 5775 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V008007
VERSION     V008007
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5775)
  AUTHORS   Olson DG, Giannone RJ, Hettich RL, Lynd LR.
  TITLE     Role of the CipA Scaffoldin Protein in Cellulose Solubilization, as
            Determined by Targeted Gene Deletion and Complementation in
            Clostridium thermocellum
  JOURNAL   J. Bacteriol. 195 (4), 733-739 (2013)
   PUBMED   23204466
REFERENCE   2  (bases 1 to 5775)
  AUTHORS   Olson DG, Lynd LR.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2012) Thayer School of Engineering, Dartmouth
            College, 14 Engineering Drive, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 5775)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5775)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Bacteriol."; date: "2013"; volume: "195"; issue: "4"; pages:
            "733-739"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (13-OCT-2012) Thayer School of Engineering, Dartmouth College, 14
            Engineering Drive, Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5775
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      28..573
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     rep_origin      829..1043
                     /label="Ctherm_ori"
                     /note="Ctherm_ori"
     CDS             1044..2045
                     /label="repB"
                     /note="RepB replication protein"
     CDS             2155..2802
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(2886..3743)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3744..3835)
                     /label="AmpR promoter"
     terminator      complement(3854..3881)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(3973..4059)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    complement(4262..4318)
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     promoter        complement(4339..4623)
                     /label="araBAD promoter"
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite
                     direction (Guzman et al., 1995)"
     CDS             4650..5525
                     /label="araC"
                     /note="L-arabinose regulatory protein"
ORIGIN
        1 ttttatctga ttaataagat gatcttcttg agatcgtttt ggtctgcgcg taatctcttg
       61 ctctgaaaac gaaaaaaccg ccttgcaggg cggtttttcg aaggttctct gagctaccaa
      121 ctctttgaac cgaggtaact ggcttggagg agcgcagtca ccaaaacttg tcctttcagt
      181 ttagccttaa ccggcgcatg acttcaagac taactcctct aaatcaatta ccagtggctg
      241 ctgccagtgg tgcttttgca tgtctttccg ggttggactc aagacgatag ttaccggata
      301 aggcgcagcg gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg
      361 cctacccgga actgagtgtc aggcgtggaa tgagacaaac gcggccataa cagcggaatg
      421 acaccggtaa accgaaaggc aggaacagga gagcgcacga gggagccgcc agggggaaac
      481 gcctggtatc tttatagtcc tgtcgggttt cgccaccact gatttgagcg tcagatttcg
      541 tgatgcttgt caggggggcg gagcctatgg aaaaacggct ttgccgcggc cctctcactt
      601 ccctgttaag tatcttcctg gcatcttcca ggaaatctcc gccccgttcg taagccattt
      661 ccgctcgccg cagtcgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaatata
      721 tcctgtatca catattctgc tgacgcaccg gtgcagcctt ttttctcctg ccacatgaag
      781 cacttcactg acaccctcat cagtgccaac atagtaagcc agtatacaaa tgagtgcttt
      841 ttttgcgttt tgagcgtagc gaaaaacgag ttctttctat tcttgataca tatagaaata
      901 acgtcatttt tattttagtt gctgaaaggt gcgttgaagt gttggtatgt atgtgtttta
      961 aagtattgaa aacccttaaa attggttgca cagaaaaacc ccatctgtta aagttataag
     1021 tgaccaaaca aataactaaa tagatggggg tttcttttaa tattatgtgt cctaatagta
     1081 gcatttattc agatgaaaaa tcaagggttt tagtggacaa gacaaaaagt ggaaaagtga
     1141 gaccatggag agaaaagaaa atcgctaatg ttgattactt tgaacttctg catattcttg
     1201 aatttaaaaa ggctgaaaga gtaaaagatt gtgctgaaat attagagtat aaacaaaatc
     1261 gtgaaacagg cgaaagaaag ttgtatcgag tgtggttttg taaatccagg ctttgtccaa
     1321 tgtgcaactg gaggagagca atgaaacatg gcattcagtc acaaaaggtt gttgctgaag
     1381 ttattaaaca aaagccaaca gttcgttggt tgtttctcac attaacagtt aaaaatgttt
     1441 atgatggcga agaattaaat aagagtttgt cagatatggc tcaaggattt cgccgaatga
     1501 tgcaatataa aaaaattaat aaaaatcttg ttggttttat gcgtgcaacg gaagtgacaa
     1561 taaataataa agataattct tataatcagc acatgcatgt attggtatgt gtggaaccaa
     1621 cttattttaa gaatacagaa aactacgtga atcaaaaaca atggattcaa ttttggaaaa
     1681 aggcaatgaa attagactat gatccaaatg taaaagttca aatgattcga ccgaaaaata
     1741 aatataaatc ggatatacaa tcggcaattg acgaaactgc aaaatatcct gtaaaggata
     1801 cggattttat gaccgatgat gaagaaaaga atttgaaacg tttgtctgat ttggaggaag
     1861 gtttacaccg taaaaggtta atctcctatg gtggtttgtt aaaagaaata cataaaaaat
     1921 taaaccttga tgacacagaa gaaggcgatt tgattcatac agatgatgac gaaaaagccg
     1981 atgaagatgg attttctatt attgcaatgt ggaattggga acggaaaaat tattttatta
     2041 aagagtagtt caacaaacgg gattgacttt taaaaaagga ttgattctaa tgaagaaagc
     2101 agacaagtaa gcctcctaaa ttcactttag ataaaaattt aggaggcata tcaaatgaac
     2161 tttaataaaa ttgatttaga caattggaag agaaaagaga tatttaatca ttatttgaac
     2221 caacaaacga cttttagtat aaccacagaa attgatatta gtgttttata ccgaaacata
     2281 aaacaagaag gatataaatt ttaccctgca tttattttct tagtgacaag ggtgataaac
     2341 tcaaatacag cttttagaac tggttacaat agcgacggag agttaggtta ttgggataag
     2401 ttagagccac tttatacaat ttttgatggt gtatctaaaa cattctctgg tatttggact
     2461 cctgtaaaga atgacttcaa agagttttat gatttatacc tttctgatgt agagaaatat
     2521 aatggttcgg ggaaattgtt tcccaaaaca cctatacctg aaaatgcttt ttctctttct
     2581 attattccat ggacttcatt tactgggttt aacttaaata tcaataataa tagtaattac
     2641 cttctaccca ttattacagc aggaaaattc attaataaag gtaattcaat atatttaccg
     2701 ctatctttac aggtacatca ttctgtttgt gatggttatc atgcaggatt gtttatgaac
     2761 tctattcagg aattgtcaga taggcctaat gactggcttt tataatatga gataatgccg
     2821 actgtacttt ttacagtcgg ttttctaatg tcactagggc tcgcctttgg gaagtttgaa
     2881 ggttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     2941 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     3001 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     3061 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     3121 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     3181 aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     3241 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     3301 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     3361 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     3421 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     3481 tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg
     3541 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     3601 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     3661 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     3721 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     3781 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaaagagt
     3841 ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttaattt gatgcctggc
     3901 agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcgcaa cgttcaaatc
     3961 cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg
     4021 aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctggcag ttccctactc
     4081 tcgcatgggg agaccccaca ctaccatcgg cgctacggcg tttcacttct gagttcggca
     4141 tggggtcagg tgggaccacc gcgctactgc cgccaggcaa attctgtttt atcagaccgc
     4201 ttctgcgttc tgatttaatc tgtatcaggc tgaaaatctt ctctcatccg ccaaaacagc
     4261 caagcttgca tgcctgcagg tcgactctag aggatccccg ggtaccgagc tcgaattcgc
     4321 tagcccaaaa aaacgggtat ggagaaacag tagagagttg cgataaaaag cgtcaggtag
     4381 gatccgctaa tcttatggat aaaaatgcta tggcatagca aagtgtgacg ccgtgcaaat
     4441 aatcaatgtg gacttttctg ccgtgattat agacactttt gttacgcgtt tttgtcatgg
     4501 ctttggtccc gctttgttac agaatgcttt taataagcgg ggttaccggt ttggttagcg
     4561 agaagagcca gtaaaagacg cagtgacggc aatgtctgat gcaatatgga caattggttt
     4621 cttctctgaa tggcgggagt atgaaaagta tggctgaagc gcaaaatgat cccctgctgc
     4681 cgggatactc gtttaatgcc catctggtgg cgggtttaac gccgattgag gccaacggtt
     4741 atctcgattt ttttatcgac cgaccgctgg gaatgaaagg ttatattctc aatctcacca
     4801 ttcgcggtca gggggtggtg aaaaatcagg gacgagaatt tgtttgccga ccgggtgata
     4861 ttttgctgtt cccgccagga gagattcatc actacggtcg tcatccggag gctcgcgaat
     4921 ggtatcacca gtgggtttac tttcgtccgc gcgcctactg gcatgaatgg cttaactggc
     4981 cgtcaatatt tgccaatacg gggttctttc gcccggatga agcgcaccag ccgcatttca
     5041 gcgacctgtt tgggcaaatc attaacgccg ggcaagggga agggcgctat tcggagctgc
     5101 tggcgataaa tctgcttgag caattgttac tgcggcgcat ggaagcgatt aacgagtcgc
     5161 tccatccacc gatggataat cgggtacgcg aggcttgtca gtacatcagc gatcacctgg
     5221 cagacagcaa ttttgatatc gccagcgtcg cacagcatgt ttgcttgtcg ccgtcgcgtc
     5281 tgtcacatct tttccgccag cagttaggga ttagcgtctt aagctggcgc gaggaccaac
     5341 gtatcagcca ggcgaagctg cttttgagca ccacccggat gcctatcgcc accgtcggtc
     5401 gcaatgttgg ttttgacgat caactctatt tctcgcgggt atttaaaaaa tgcaccgggg
     5461 ccagcccgag cgagttccgt gccggttgtg aagaaaaagt gaatgatgta gccgtcaagt
     5521 tgtcataatt ggtaacgaat cagacaattg acggcttgac ggagtagcat agggtttgca
     5581 gaatccctgc ttcgtccatt tgacaggcac attatgcatc gatgataagc tgtcaaacat
     5641 gagcagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
     5701 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
     5761 atgagcctag aaata
//
