LOCUS       V013236                10715 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013236
VERSION     V013236
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10715)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10715
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(644..1015)
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     CDS             complement(1102..2181)
                     /label="lacI"
                     /note="lac repressor"
     CDS             complement(2434..3462)
                     /label="Cre"
                     /note="site-specific recombinase"
     CDS             7344..8075
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     rep_origin      complement(8886..9474)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9648..10505)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10506..10610)
                     /label="AmpR promoter"
ORIGIN
        1 aacaaaattc tccagtcttc acatcggttt gaaaggagga agcggaagaa tgaagtaaga
       61 gggatttttg actccgaagt aagtcttcaa aaaatcaaat aaggagtgtc aagaatgttt
      121 gcaaaacgat tcaaaacctc tttactgccg ttattcgctg gatttttatt gctgtttcat
      181 ttggttctgg caggaccggc ggctgcgagt gctgaaacgg cgaacaaatc gaatgagctt
      241 acagcaccgt cgatcaaaag cggaaccatt cttcatgcat ggaattggtc gttcaatacg
      301 ttaaaacaca atatgaagga tattcatgat gcaggatata cagccattca gacatctccg
      361 attaaccaag taaaggaagg gaatcaagga gataaaagca tgtcgaactg gtactggctg
      421 tatcagccga catcgtatca aattggcaac cgttacttag gtactgaaca agaatttaaa
      481 gaaatgtgtg cagccgctga agaatatggc ataaaggtca ttgttgacgc ggtcatcaat
      541 cataccacca gtgattatgc cgcgatttcc aatgaggtta agagtattcc aaactggaca
      601 catggaaaca cacaaattaa aaactggtct gatcggatcc tcagtcctgc tcctcggcca
      661 cgaagtgcac gcagttgccg gccgggtcgc gcagggcgaa ctcccgcccc cacggctgct
      721 cgccgatctc ggtcatggcc ggcccggagg cgtcccggaa gttcgtggac acgacctccg
      781 accactcggc gtacagctcg tccaggccgc gcacccacac ccaggccagg gtgttgtccg
      841 gcaccacctg gtcctggacc gcgctgatga acagggtcac gtcgtcccgg accacaccgg
      901 cgaagtcgtc ctccacgaag tcccgggaga acccgagccg gtcggtccag aactcgaccg
      961 ctccggcgac gtcgcgcgcg gtgagcaccg gaacggcact ggtcaacttg gccatattta
     1021 gttcctcctt tctgtatgta gagtaacaca tataaaaagc catatcaagg atcctaactc
     1081 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     1141 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt
     1201 ttttcttttc accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga
     1261 gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt
     1321 ggttgacggc gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat
     1381 atccgcacca acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg
     1441 atcgttggca accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg
     1501 ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt
     1561 gcgagtgaga tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg
     1621 gcccgctaac agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg
     1681 cgtaccgtct tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag
     1741 aaataacgcc ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag
     1801 cggatagtta atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt
     1861 acaggcttcg acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc
     1921 ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt
     1981 ggcaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat
     2041 gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct
     2101 ggcctggttc accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc
     2161 gtataacgtt actggtttca tcaaaatcgt ctccctccgt ttgaatattt gattgatcgt
     2221 aaccagatga agcactcttt ccactatccc tacagtgtta tggcttgaac aatcacgaaa
     2281 caataattgg tacgtacgat ctttcagccg actcaaacat caaatcttac aaatgtagtc
     2341 tttgaaagta ttacatatgt aagatttaaa tgcaaccgtt ttttcggaag gaaatgatga
     2401 cctcgtttcc accggaatta gcttgcatgc ctaatcgcca tcttccagca ggcgcaccat
     2461 tgcccctgtt tcactatcca ggttacggat atagttcatg acaatattta cattggtcca
     2521 gccaccagct tgcatgatct ccggtattga aactccagcg cgggccatat ctcgcgcggc
     2581 tccgacacgg gcactgtgtc cagaccaggc caggtatctc tgaccagagt catccttagc
     2641 gccgtaaatc aatcgatgag ttgcttcaaa aatcccttcc agggcgcgag ttgatagctg
     2701 gctggtggca gatggcgcgg caacaccatt ttttctgacc cggcaaaaca ggtagttatt
     2761 cggatcatca gctacaccag agacggaaat ccatcgctcg accagtttag ttacccccag
     2821 gctaagtgcc ttctctacac ctgcggtgct aaccagcgtt ttcgttctgc caatatggat
     2881 taacattctc ccaccgtcag tacgtgagat atctttaacc ctgatcctgg caatttcggc
     2941 tatacgtaac agggtgttat aagcaatccc cagaaatgcc agattacgta tatcctggca
     3001 gcgatcgcta ttttccatga gtgaacgaac ctggtcgaaa tcagtgcgtt cgaacgctag
     3061 agcctgtttt gcacgttcac cggcatcaac gttttctttt cggatccgcc gcataaccag
     3121 tgaaacagca ttgctgtcac ttggtcgtgg cagcccggac cgacgatgaa gcatgtttag
     3181 ctggcccaaa tgttgctgga tagtttttac tgccagaccg cgcgcctgaa gatatagaag
     3241 ataatcgcga acatcttcag gttctgcggg aaaccatttc cggttattca acttgcacca
     3301 tgccgcccac gaccggcaaa cggacagaag cattttccag gtatgctcag aaaacgcctg
     3361 gcgatccctg aacatgtcca tcaggttctt gcgaacctca tcactcgttg catcgaccgg
     3421 taatgcaggc aaattttggt gtacggtcag taaattggac atacctgctt cctccttaag
     3481 cttaattgtt atccgctcac aattccacac attatgccac accttgtaga taaagtcaac
     3541 aactttttgc aaaatttttc aggaatttta gcagaggttg ttctggatgt agaacaaaac
     3601 atctttccgc tcttgtgctg ttaggatatc tttcttggaa gctaggtagg caagggctac
     3661 ctcaaataaa tcttcttcag ggtgcgctat ttttaaggtg cctagtgagg tcttgaccac
     3721 ttcacccata atttcagtgc cgaatagtct ggactgggct gtgtagaatt cctgcagccc
     3781 tggcgaatgg cgattttcgt tcgtgaatac atgttataat aactataact aataacgtaa
     3841 cgtgactggc aagagatatt tttaaaacaa tgaataggtt tacacttact ttagttttat
     3901 ggaaatgaaa gatcatatca tatataatct agaataaaat taactaaaat aattattatc
     3961 tagataaaaa atttagaagc caatgaaatc tataaataaa ctaaattaag tttatttaat
     4021 taacaactat ggatataaaa taggtactaa tcaaaatagt gaggaggata tatttgaata
     4081 catacgaaca aattaataaa gtgaaaaaaa tacttcggaa acatttaaaa aataacctta
     4141 ttggtactta catgtttgga tcaggagttg agagtggact aaaaccaaat agtgatcttg
     4201 actttttagt cgtcgtatct gaaccattga cagatcaaag taaagaaata cttatacaaa
     4261 aaattagacc tatttcaaaa aaaataggag ataaaagcaa cttacgatat attgaattaa
     4321 caattattat tcagcaagaa atggtaccgt ggaatcatcc tcccaaacaa gaatttattt
     4381 atggagaatg gttacaagag ctttatgaac aaggatacat tcctcagaag gaattaaatt
     4441 cagatttaac cataatgctt taccaagcaa aacgaaaaaa taaaagaata tacggaaatt
     4501 atgacttaga ggaattacta cctgatattc cattttctga tgtgagaaga gccattatgg
     4561 attcgtcaga ggaattaata gataattatc aggatgatga aaccaactct atattaactt
     4621 tatgccgtat gattttaact atggacacgg gtaaaatcat accaaaagat attgcgggaa
     4681 atgcagtggc tgaatcttct ccattagaac atagggagag aattttgtta gcagttcgta
     4741 gttatcttgg agagaatatt gaatggacta atgaaaatgt aaatttaact ataaactatt
     4801 taaataacag attaaaaaaa ttataaaaaa attgaaaaaa tggtggaaac acttttttca
     4861 atttttttgt tttattattt aatatttggg aaatattcat tctaattggt aatcagattt
     4921 tagaaaacaa taaacccttg cataggggga tctcgacatg gatgagcgat gatgatatcc
     4981 gtttaggctg ggcggtgata gcttctcgtt caggcagtac gcctcttttc ttttccagac
     5041 ctgagggagg cggaaatggt gtgaggttcc cggggaaaag ccaaataggc gatcgcggga
     5101 gtgctttatt tgaagatcag gctatcactg cggtcaatag atttcacaat gtgatggctg
     5161 gacagcctga ggaactctcg aacccgaatg gaaacaacca gatatttatg aatcagcgcg
     5221 gctcacatgg cgttgtgctg gcaaatgcag gttcatcctc tgtctctatc aatacggcaa
     5281 caaaattgcc tgatggcagg tatgacaata aagctggagc gggttcattt caagtgaacg
     5341 atggtaaact gacaggcacg atcaatgcca ggtctgtagc tgtgctttat cctgatgata
     5401 ttgcaaaagc gcctcatgtt ttccttgaga attacaaaac aggtgtaaca cattctttca
     5461 atgatcaact gacgattacc ttgcgtgcag atgcgaatac aacaaaagcc gtttatcaaa
     5521 tcaataatgg accagacgac aggcgtttaa ggatggagat caattcacaa tcggaaaagg
     5581 agatccaatt tggcaaaaca tacaccatca tgttaaaagg aacgaacagt gatggtgtaa
     5641 cgaggaccga gaaatacagt tttgttaaaa gagatccagc gtcggccaaa accatcggct
     5701 atcaaaatcc gaatcattgg agccaggtaa atgcttatat ctataaacat gatgggagcc
     5761 gagtaattga attgaccgga tcttggcctg gaaaaccaat gactaaaaat gcagacggaa
     5821 tttacacgct gacgctgcct gcggacacgg atacaaccaa cgcaaaagtg atttttaata
     5881 atggcagcgc ccaagtgccc ggtcagaatc agcctggctt tgattacgtg ctaaatggtt
     5941 tatataatga ctcgggctta agcggttctc ttccccattg agggcaaggc tagacgggac
     6001 ttaccgaaag aaaccatcaa tgatggtttc ttttttgttc ataaatcaga caaaactttt
     6061 ctcttgcaaa agtttgtgaa gtgttgcaca atataaatgt gaaatacttc acaaacaaaa
     6121 agacatcaaa gagaaacata ccctgcaagg atgctgatat tgtctgcatt tgcgccggag
     6181 caaaccaaaa acctggtgag acacgccttg aattagtaga aaagaacttg aagattttca
     6241 aaggcatcgt tagtgaagtc atggcgagcg gatttgacgg cattttctta gtcgcgacgc
     6301 gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg
     6361 cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat
     6421 cgctcgcggc tcttaccagc ctaacttcga tcactggacc gctgatcgtc acggcgattt
     6481 atgccgcctc ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc gccctatacc
     6541 ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg ggccacctac tgaagtggat
     6601 ttctttaaga gctcctttaa cttcctcacc agtagttgta tcggtaccat aagtagaagc
     6661 agcaacccaa gtagctttac cagcatccgg ttcaaccagc atagtaagaa tcttactgga
     6721 catcggcagt tcttcgaaca gtgcgccaac taccagctct ttctgcagtt cattcagggc
     6781 accggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc
     6841 tcttgatcca tggattacgc gttaacccgg gcccgcggat gcatatgatc agatccttta
     6901 actctggcaa ccctcaaaat tgaatgagac atgctacacc tccggataat aaatatatat
     6961 aaacgtatat agatttcata aagtctaaca cactagactt atttacttcg taattaagtc
     7021 gttaaaccgt gtgctctacg accaaaacta taaaaccttt aagaactttc tttttttaca
     7081 agaaaaaaga aattagataa atctctcata tcttttattc aataatcgca tccgattgca
     7141 gtataaattt aacgatcact catcatgttc atatttatca gagctcgtgc tataattata
     7201 ctaattttat aaggaggaaa aaatatgggc atttttagta tttttgtaat cagcacagtt
     7261 cattatcaac caaacaaaaa ataagtggtt ataatgaatc gttaataagc aaaattcata
     7321 taaccaaatt aaagagggtt ataatgaacg agaaaaatat aaaacacagt caaaacttta
     7381 ttacttcaaa acataatata gataaaataa tgacaaatat aagattaaat gaacatgata
     7441 atatctttga aatcggctca ggaaaaggcc attttaccct tgaattagta aagaggtgta
     7501 atttcgtaac tgccattgaa atagaccata aattatgcaa aactacagaa aataaacttg
     7561 ttgatcacga taatttccaa gttttaaaca aggatatatt gcagtttaaa tttcctaaaa
     7621 accaatccta taaaatatat ggtaatatac cttataacat aagtacggat ataatacgca
     7681 aaattgtttt tgatagtata gctaatgaga tttatttaat cgtggaatac gggtttgcta
     7741 aaagattatt aaatacaaaa cgctcattgg cattactttt aatggcagaa gttgatattt
     7801 ctatattaag tatggttcca agagaatatt ttcatcctaa acctaaagtg aatagctcac
     7861 ttatcagatt aagtagaaaa aaatcaagaa tatcacacaa agataaacaa aagtataatt
     7921 atttcgttat gaaatgggtt aacaaagaat acaagaaaat atttacaaaa aatcaattta
     7981 acaattcctt aaaacatgca ggaattgacg atttaaacaa tattagcttt gaacaattct
     8041 tatctctttt caatagctat aaattattta ataagtaagt taagggatgc ataaactgca
     8101 tcccttaact tgtttttcgt gtgcctattt tttgtgaatc gattatgtct tttgcgcagt
     8161 cggcttaaac cagttttcgc tggtgcgaaa aaagagtgtc ttgtgacacc taaattcaaa
     8221 atctatcggt cagatttata ccgatttgat tttatatatt cttgaataac atacgccgag
     8281 ttatcacata aaagcgggaa ccaatcatca aatttaaact tcattgcata atccattaaa
     8341 ctcttaaatt ctacgattcc ttgttcatca ataaactcaa tcatttcttt aattaattta
     8401 tatctatctg ttgttgtttt ctttaataat tcatcaacat ctacaccgcc ataaactatc
     8461 atatcttctt tttgatattt aaatttatta ggatcttaag gcctaggtct agagtctttg
     8521 ttttgacgcc attagcgtac gtaacaatcc tcgttaaagg acaaggacct gagcggaagt
     8581 gtatcgtaca gtagacggag tatactagta tagtctatag tccgtggaat tattatattt
     8641 atctccgacg atattctcat cagtgaaatc cagctggagt tctttagcaa atttttttat
     8701 tagctgaact tagtattagt ggccatactc ctccaatcca aagctattta gaaagattac
     8761 tatatcctca aacaggcggt aaccggcctc ttcatcggga atgcgcgcga ccttcagcat
     8821 cgccggcatg tccccctggc ggacgggaag tatccagctc gaggtcgggc cgcgttgctg
     8881 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     8941 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     9001 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     9061 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     9121 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     9181 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     9241 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     9301 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     9361 gttaccttcg gaaaaagagt tgatagctct tgatccggca aacaaaccac cgctggtagc
     9421 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     9481 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     9541 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     9601 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     9661 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     9721 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     9781 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     9841 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     9901 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     9961 gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    10021 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    10081 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    10141 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    10201 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    10261 acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    10321 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    10381 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    10441 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    10501 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    10561 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    10621 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
    10681 aggcgtatca cgaggccctt tcgtcttcaa gaatt
//
