LOCUS       Exported                6277 bp DNA     circular SYN 13-OCT-2025
DEFINITION  Exported.
ACCESSION   V013018
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6277)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6277)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6277)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6277
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             226..873
                     /gene="cat"
                     /label=Chloramphenicol acetyltransferase
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             1256..2272
                     /codon_start=1
                     /label=amyE-R
                     /label=alpha-amylase
                     /translation="MSDDDIRLGWAVIASRSGSTPLFFSRPEGGGNGVRFPGKSQIGDR
                     GSALFEDQAITAVNRFHNVMAGQPEELSNPNGNNQIFMNQRGSHGVVLANAGSSSVSIN
                     TATKLPDGRYDNKAGAGSFQVNDGKLTGTINARSVAVLYPDDIAKAPHVFLENYKTGVT
                     HSFNDQLTITLRADANTTKAVYQINNGPETAFKDGDQFTIGKGDPFGKTYTIMLKGTNS
                     DGVTRTEKYSFVKRDPASAKTIGYQNPNHWSQVNAYIYKHDGSRVIELTGSWPGKPMTK
                     NADGIYTLTLPADTDTTNAKVIFNNGSAQVPGQNQPGFDYVLNGLYNDSGLSGSLPH"
     rep_origin      complement(3577..4165)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4339..5196)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5197..5301)
                     /label=AmpR promoter
     CDS             5521..6063
                     /codon_start=1
                     /label=amyE-F
                     /translation="MFAKRFKTSLLPLFAGFLLLFHLVLAGPAAASAETANKSNELTAP
                     SIKSGTILHAWNWSFNTLKHNMKDIHDAGYTAIQTSPINQVKEGNQGDKSMSNWYWLYQ
                     PTSYQIGNRYLGTEQEFKEMCAAAEEYGIKVIVDAVINHTTSDYAAISNEVKSIPNWTH
                     GNTQIKNWSDRWDVTQN"
ORIGIN
        1 ctttaaaaaa acacaaaaga ccacattttt taatgtggtc ttttattctt caactaaagc
       61 acccattagt tcaacaaacg aaaattggat aaagtgggat atttttaaaa tatatattta
      121 tgttacagta atattgactt ttaaaaaagg attgattcta atgaagaaag cagacaagta
      181 agcctcctaa attcacttta gataaaaatt taggaggcat atcaaatgaa ctttaataaa
      241 attgatttag acaattggaa gagaaaagag atatttaatc attatttgaa ccaacaaacg
      301 acttttagta taaccacaga aattgatatt agtgttttat accgaaacat aaaacaagaa
      361 ggatataaat tttaccctgc atttattttc ttagtgacaa gggtgataaa ctcaaataca
      421 gcttttagaa ctggttacaa tagcgacgga gagttaggtt attgggataa gttagagcca
      481 ctttatacaa tttttgatgg tgtatctaaa acattctctg gtatttggac tcctgtaaag
      541 aatgacttca aagagtttta tgatttatac ctttctgatg tagagaaata taatggttcg
      601 gggaaattgt ttcccaaaac acctatacct gaaaatgctt tttctctttc tattattcca
      661 tggacttcat ttactgggtt taacttaaat atcaataata atagtaatta ccttctaccc
      721 attattacag caggaaaatt cattaataaa ggtaattcaa tatatttacc gctatcttta
      781 caggtacatc attctgtttg tgatggttat catgcaggat tgtttatgaa ctctattcag
      841 gaattgtcag ataggcctaa tgactggctt ttataatatg agataatgcc gactgtactt
      901 tttacagtcg gttttctaat gtcactaacc tgccccgtta gttgaagaag gtttttatat
      961 tacagctcca gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg
     1021 cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg
     1081 ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt
     1141 tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc
     1201 tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga catggatgag
     1261 cgatgatgat atccgtttag gctgggcggt gatagcttct cgttcaggca gtacgcctct
     1321 tttcttttcc agacctgagg gaggcggaaa tggtgtgagg ttcccgggga aaagccaaat
     1381 aggcgatcgc gggagtgctt tatttgaaga tcaggctatc actgcggtca atagatttca
     1441 caatgtgatg gctggacagc ctgaggaact ctcgaacccg aatggaaaca accagatatt
     1501 tatgaatcag cgcggctcac atggcgttgt gctggcaaat gcaggttcat cctctgtctc
     1561 tatcaatacg gcaacaaaat tgcctgatgg caggtatgac aataaagctg gagcgggttc
     1621 atttcaagtg aacgatggta aactgacagg cacgatcaat gccaggtctg tagctgtgct
     1681 ttatcctgat gatattgcaa aagcgcctca tgttttcctt gagaattaca aaacaggtgt
     1741 aacacattct ttcaatgatc aactgacgat taccttgcgt gcagatgcga atacaacaaa
     1801 agccgtttat caaatcaata atggaccaga gacggcgttt aaggatggag atcaattcac
     1861 aatcggaaaa ggagatccat ttggcaaaac atacaccatc atgttaaaag gaacgaacag
     1921 tgatggtgta acgaggaccg agaaatacag ttttgttaaa agagatccag cgtcggccaa
     1981 aaccatcggc tatcaaaatc cgaatcattg gagccaggta aatgcttata tctataaaca
     2041 tgatgggagc cgagtaattg aattgaccgg atcttggcct ggaaaaccaa tgactaaaaa
     2101 tgcagacgga atttacacgc tgacgctgcc tgcggacacg gatacaacca acgcaaaagt
     2161 gatttttaat aatggcagcg cccaagtgcc cggtcagaat cagcctggct ttgattacgt
     2221 gctaaatggt ttatataatg actcgggctt aagcggttct cttccccatt gagggcaagg
     2281 ctagacggga cttaccgaaa gaaaccatca atgatggttt cttttttgtt cataaatcag
     2341 acaaaacttt tctcttgcaa aagtttgtga agtgttgcac aatataaatg tgaaatactt
     2401 cacaaacaaa aagacatcaa agagaaacat accctgcaag gatgctgata ttgtctgcat
     2461 ttgcgccgga gcaaaccaaa aacctggtga gacacgcctt gaattagtag aaaagaactt
     2521 gaagattttc aaaggcatcg ttagtgaagt catggcgagc ggatttgacg gcattttctt
     2581 agtcgcgacg cgaggctgga tggccttccc cattatgatt cttctcgctt ccggcggcat
     2641 cgggatgccc gcgttgcagg ccatgctgtc caggcaggta gatgacgacc atcagggaca
     2701 gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg atcattggac cgctgatcgt
     2761 cacggcgatt tatgccgcct cggcgagcac atggaacggg ttggcatgga ttgtaggcgc
     2821 cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt gcatggagcc gggccaccta
     2881 ctgaagtgga tttctttaag agctccttta acttcctcac cagtagttgt atcggtacca
     2941 taagtagaag cagcaaccca agtagcttta ccagcatccg gttcaaccag catagtaaga
     3001 atcttactgg acatcggcag ttcttcgaac agtgcgccaa ctaccagctc tttctgcagt
     3061 tcattcaggg caccggagaa cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc
     3121 ctcatcctgt ctcttgatcc atggattacg cgttaacccg ggcccgcgga tgcatatgat
     3181 cagatcttaa ggcctaggtc tagagtcttt gttttgacgc cattagcgta cgtaacaatc
     3241 ctcgttaaag gacaaggacc tgagcggaag tgtatcgtac agtagacgga gtatactagt
     3301 atagtctata gtccgtggaa ttattatatt tatctccgac gatattctca tcagtgaaat
     3361 ccagctggag ttctttagca aattttttta ttagctgaac ttagtattag tggccatact
     3421 cctccaatcc aaagctattt agaaagatta ctatatcctc aaacaggcgg taaccggcct
     3481 cttcatcggg aatgcgcgcg accttcagca tcgccggcat gtccccctgg cggacgggaa
     3541 gtatccagct cgaggtcggg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     3601 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     3661 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     3721 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     3781 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     3841 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     3901 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     3961 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     4021 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     4081 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     4141 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     4201 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     4261 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     4321 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     4381 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     4441 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     4501 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     4561 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     4621 taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt
     4681 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     4741 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     4801 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     4861 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     4921 gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag
     4981 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     5041 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     5101 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     5161 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     5221 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     5281 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     5341 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca
     5401 agaattaaca aaattctcca gtcttcacat cggtttgaaa ggaggaagcg gaagaatgaa
     5461 gtaagaggga tttttgactc cgaagtaagt cttcaaaaaa tcaaataagg agtgtcaaga
     5521 atgtttgcaa aacgattcaa aacctcttta ctgccgttat tcgctggatt tttattgctg
     5581 tttcatttgg ttctggcagg accggcggct gcgagtgctg aaacggcgaa caaatcgaat
     5641 gagcttacag caccgtcgat caaaagcgga accattcttc atgcatggaa ttggtcgttc
     5701 aatacgttaa aacacaatat gaaggatatt catgatgcag gatatacagc cattcagaca
     5761 tctccgatta accaagtaaa ggaagggaat caaggagata aaagcatgtc gaactggtac
     5821 tggctgtatc agccgacatc gtatcaaatt ggcaaccgtt acttaggtac tgaacaagaa
     5881 tttaaagaaa tgtgtgcagc cgctgaagaa tatggcataa aggtcattgt tgacgcggtc
     5941 atcaatcata ccaccagtga ttatgccgcg atttccaatg aggttaagag tattccaaac
     6001 tggacacatg gaaacacaca aattaaaaac tggtctgatc gatgggatgt cacgcagaat
     6061 taaatagtac ataatggatt tccttacgcg aaatacgggc agacatggcc tgcccggtta
     6121 ttattatttt tgacaccaga ccaactggta atggtagcga ccggcgctca ggatcctaga
     6181 agcttatcga attctcatgt ttgacagctt atcatcggca atagttaccc ttattatcaa
     6241 gataagaaag aaaaggattt ttcgctacgc tcaaatc
//
