LOCUS       V008014                 8189 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V008014
VERSION     V008014
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8189)
  AUTHORS   Guo D, Hazbun TR, Xu XJ, Ng SL, Fields S, Kuo MH.
  TITLE     A tethered catalysis, two-hybrid system to identify protein-protein
            interactions requiring post-translational modifications
  JOURNAL   Nat. Biotechnol. 22 (7), 888-892 (2004)
   PUBMED   15208639
REFERENCE   2  (bases 1 to 8189)
  AUTHORS   Kuo M-H., Guo D.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2004) Biochem. Mol. Biol., Michigan State
            University, 309 BCH Building, East Lansing, MI 48824, USA
REFERENCE   3  (bases 1 to 8189)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8189)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat.
            Biotechnol."; date: "2004"; volume: "22"; issue: "7"; pages:
            "888-892"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (08-JUN-2004) Biochem. Mol. Biol., Michigan State University, 309
            BCH Building, East Lansing, MI 48824, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8189
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             434..874
                     /label="GAL4 DNA binding domain"
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
     CDS             944..1018
                     /note="Histone H4 from Medicago sativa. Accession#: P62789"
                     /label="Histone H4"
     CDS             1097..1108
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     CDS             1850..1939
                     /label="3xHA"
                     /note="three tandem HA epitope tags"
     terminator      2378..2565
                     /label="ADH1 terminator"
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     misc_feature    complement(3397..4559)
                     /label="CEN/ARS"
                     /note="S. cerevisiae CEN4 centromere fused to the
                     autonomously replicating sequence ARS1/ARS416"
     promoter        complement(5073..5174)
                     /label="TRP1 promoter"
     promoter        5280..5384
                     /label="AmpR promoter"
     CDS             5385..6242
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6416..7004
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gtggtacata acgaactaat actgtagccc tagacttgat agccatcatc atatcgaagt
       61 ttcactaccc tttttccatt tgccatctat tgaagtaata ataggcgcat gcaacttctt
      121 ttcttttttt ttcttttctc tctcccccgt tgttgtctca ccatatccgc aatgacaaaa
      181 aaatgatgga agacactaaa ggaaaaaatt aacgacaaag acagcaccaa cagatgtcgt
      241 tgttccagag ctgatgaggg gtatctcgaa gcacacgaaa ctttttcctt ccttcattga
      301 cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt
      361 ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa gcttgaagca
      421 agcctcctga aagatgaagc tactgtcttc tatcgaacaa gcatgcgata tttgccgact
      481 taaaaagctc aagtgctcca aagaaaaacc gaagtgcgcc aagtgtctga agaacaactg
      541 ggagtgtcgc tactctccca aaaccaaaag gtctccgctg actagggcac atctgacaga
      601 agtggaatca aggctagaaa gactggaaca gctatttcta ctgatttttc ctcgagaaga
      661 ccttgacatg attttgaaaa tggattcttt acaggatata aaagcattgt taacaggatt
      721 atttgtacaa gataatgtga ataaagatgc cgtcacagat agattggctt cagtggagac
      781 tgatatgcct ctaacattga gacagcatag aataagtgcg acatcatcat cggaagagag
      841 tagtaacaaa ggtcaaagac agttgactgt atcgaactat ctattcgatg atgaagatac
      901 cccaccaaac ccaaaaaaag agatcgaatt ccagctgacc accatgtccg gtagaggtaa
      961 aggtggtaaa ggtctaggaa aaggtggtgc caagcgtcac agaaagattc taagagataa
     1021 catccaaggt atttccgggt cccctatact aggttattgg aaaggtcgac gcgaccatcc
     1081 tccaaaatcg gatctgatcg aaggtcgtgg agatcccgaa gttaaacggg taaaattaga
     1141 aaacaacgtt gaagaaatac aacctgagca ggctgagacc aataaacaag agggcaccga
     1201 taaagagaat aaaggaaagt tcgagaaaga aactgagaga ataggaggat ctgaagtggt
     1261 tacagatgtg gaaaaaggaa ttgtcaaatt tgaatttgat ggtgttgaat acacattcaa
     1321 agagagaccc agtgtcgtag aggaaaatga aggtaaaatt gagtttaggg tggtgaataa
     1381 tgataatact aaagaaaaca tgatggtcct aactggatta aaaaacattt ttcaaaagca
     1441 attaccaaaa atgcccaaag aatacattgc caggttagtc tatgatcgaa gtcatctttc
     1501 catggctgtc attaggaagc cattgactgt cgtaggtggc ataacatatc gacctttcga
     1561 taagagagaa ttcgcagaaa ttgttttctg tgccatcagt tcgacggaac aggtacgcgg
     1621 ttatggtgcg catctaatga atcacttaaa agactatgtt agaaatacct cgaacataaa
     1681 atattttttg acatatgcag ataattacgc tattggatac tttaaaaagc aaggctttac
     1741 taaagaaatc acgttggata aaagtatatg gatgggatat attaaagatt atgaaggtgg
     1801 tacgctgatg caatgttcca tggcaattcc cggtggcggc cgcatctttt acccatacga
     1861 tgttcctgac tatgcgggct atccctatga cgtcccggac tatgcaggat cctatccata
     1921 tgacgttcca gattacgctg ctcagtgcgg ccgctctagc tagaactagt ggatcccccg
     1981 ataccgtcga cctgcagaga tctatgaatc gtagatactg aaaaaccccg caagttcact
     2041 tcaactgtgc atcgtgcacc atctcaattt ctttcattta tacatcgttt tgccttcttt
     2101 tatgtaacta tactcctcta agtttcaatc ttggccatgt aacctctgat ctatagaatt
     2161 ttttaaatga ctagaattaa tgcccatctt ttttttggac ctaaattctt catgaaaata
     2221 tattacgagg gcttattcag aagctttgga cttcttcgcc agaggtttgg tcaagtctcc
     2281 aatcaaggtt gtcggcttgt ctaccttgcc agaaatttac gaaaagatgg aaaagggtca
     2341 aatcgttggt agatacgttg ttgacacttc taaataagcg aatttcttat gatttatgat
     2401 ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta
     2461 ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca ggttgctttc
     2521 tcaggtatag catgaggtcg ctcttattga ccacacctct accggcatgc cgagcaaatg
     2581 cctgcaaatc gctccccatt tcacccaatt gtagatatgc taactccagc aatgagttga
     2641 tgaatctcgg tgtgtatttt atgtcctcag aggacaacac ctgttgtaat cgttcttcca
     2701 cacggatcct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     2761 agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     2821 tcacaccgca tatatcgctg ggccattctc atgaagaata tcttgaattt attgtcatat
     2881 tactagttgg tgtggaagtc catatatcgg tgatcaatat agtggttgac atgctggcta
     2941 gtcaacattg agccttttga tcatgcaaat atattacggt attttacaat caaatatcaa
     3001 acttaactat tgactttata acttatttag gtggtaacat tcttataaaa aagaaaaaaa
     3061 ttactgcaaa acagtactag cttttaactt gtatcctagg ttatctatgc tgtctcacca
     3121 tagagaatat tacctatttc agaatgtatg tccatgattc gccgggtaaa tacatataat
     3181 acacaaatct ggcttaataa agtctataat atatctcata aagaagtgct aaattggcta
     3241 gtgctatata tttttaagaa aatttctttt gactaagtcc atatcgactt tgtaaaagtt
     3301 cactttagca tacatatatt acacgagcca gaaattgtaa cttttgccta aaatcacaaa
     3361 ttgcaaaatt taattgcttg caaaaggtca catgcttata atcaactttt ttaaaaattt
     3421 aaaatacttt tttatttttt atttttaaac ataaatgaaa taatttattt attgtttatg
     3481 attaccgaaa cataaaacct gctcaagaaa aagaaactgt tttgtccttg gaaaaaaagc
     3541 actacctagg agcggccaaa atgccgaggc tttcatagct taaactcttt acagaaaata
     3601 ggcattatag atcagttcga gttttcttat tcttccttcc ggttttatcg tcacagtttt
     3661 acagtaaata agtatcacct cttagagttc gatgataagc tgtcaaacat gagaattaat
     3721 tccacatgtt aaaatagtga aggagcatgt tcggcacaca gtggaccgaa cgtggggtaa
     3781 gtgcactagg gtccggttaa acggatctcg cattgatgag gcaacgctaa ttatcaacat
     3841 atagattgtt atctatctgc atgaacacga aatctttact tgacgacttg aggctgatgg
     3901 tgtttatgca aagaaaccac tgtgtttaat atgtgtcact gtttgatatt actgtcagcg
     3961 tagaagataa tagtaaaagc ggttaataag tgtatttgag ataagtgtga taaagttttt
     4021 acagcgaaaa gacgataaat acaagaaaat gattacgagg atacggagag aggtatgtac
     4081 atgtgtattt atatactaag ctgccggcgg ttgtttgcaa gaccgagaaa aggctagcaa
     4141 gaatcgggtc attgtagcgt atgcgcctgt gaacattctc ttcaacaagt ttgattccat
     4201 tgcggtgaaa tggtaaaagt caaccccctg cgatgtatat tttcctgtac aatcaatcaa
     4261 aaagccaaat gatttagcat tatctttaca tcttgttatt ttacagattt tatgtttaga
     4321 tcttttatgc ttgcttttca aaaggcttgc aggcaagtgc acaaacaata cttaaataaa
     4381 tactactcag taataaccta tttcttagca tttttgacga aatttgctat tttgttagag
     4441 tcttttacac catttgtctc cacacctccg cttacatcaa caccaataac gccatttaat
     4501 ctaagcgcat caccaacatt ttctggcgtc agtccaccag ctaacataaa atgtaagctc
     4561 tcggggctct cttgccttcc aacccagtca gaaatcgagt tccaatccaa aagttcacct
     4621 gtcccacctg cttctgaatc aaacaaggga ataaacgaat gaggtttctg tgaagctgca
     4681 ctgagtagta tgttgcagtc ttttggaaat acgagtcttt taataactgg caaaccgagg
     4741 aactcttggt attcttgcca cgactcatct ccatgcagtt ggacgatatc aatgccgtaa
     4801 tcattgacca gagccaaaac atcctcctta ggttgattac gaaacacgcc aaccaagtat
     4861 ttcggagtgc ctgaactatt tttatatgct tttacaagac ttgaaatttt ccttgcaata
     4921 accgggtcaa ttgttctctt tctattgggc acacatataa tacccagcaa gtcagcatcg
     4981 gaatctagtg cacattctgc ggcctctgtg ctctgcaagc cgcaaacttt caccaatgga
     5041 ccagaactac ctgtgaaatt aataacagac atactccaag ctgcctttgt gtgcttaatc
     5101 acgtatactc acgtgctcaa tagtcaccaa tgccctccct cttggccctc tccttttctt
     5161 ttttcgaccg aattaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg
     5221 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     5281 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     5341 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     5401 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     5461 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     5521 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     5581 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     5641 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     5701 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     5761 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     5821 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     5881 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     5941 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     6001 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     6061 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     6121 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     6181 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     6241 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     6301 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     6361 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     6421 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     6481 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     6541 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     6601 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     6661 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     6721 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     6781 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     6841 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6901 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6961 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     7021 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     7081 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     7141 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     7201 aaccgcctct ccccgcgcgt tggccgattc attaatgcag gatccgggat cgaagaaatg
     7261 atggtaaatg aaataggaaa tcaaggagca tgaaggcaaa agacaaatat aagggtcgaa
     7321 cgaaaaataa agtgaaaagt gttgatatga tgtatttggc tttgcggcgc cgaaaaaacg
     7381 agtttacgca attgcacaat catgctgact ctgtggcgga cccgcgctct tgccggcccg
     7441 gcgataacgc tgggcgtgag gctgtgcccg gcggagtttt ttgcgcctgc attttccaag
     7501 gtttaccctg cgctaagggg cgagattgga gaagcaataa gaatgccggt tggggttgcg
     7561 atgatgacga ccacgacaac tggtgtcatt atttaagttg ccgaaagaac ctgagtgcat
     7621 ttgcaacatg agtatactag aagaatgagc caagacttgc gagacgcgag tttgccggtg
     7681 gtgcgaacaa tagagcgacc atgaccttga aggtgagacg cgcataaccg ctagagtact
     7741 ttgaagagga aacagcaata gggttgctac cagtataaat agacaggtac atacaacact
     7801 ggaaatggtt gtctgtttga gtacgctttc aattcatttg ggtgtgcact ttattatgtt
     7861 acaatatgga agggaacttt acacttctcc tatgcacata tattaattaa agtccaatgc
     7921 tagtagagaa ggggggtaac acccctccgc gctcttttcc gatttttttc taaaccgtgg
     7981 aatatttcgg atatcctttt gttgtttccg ggtgtacaat atggacttcc tcttttctgg
     8041 caaccaaacc catacatcgg gattcctata ataccttcgt tggtctccct aacatgtagg
     8101 tggcggaggg gagatataca atagaacaga taccagacaa gacataatgg gctaaacaag
     8161 actacaccaa ttacactgcc tcattgatg
//
