LOCUS       V008015                 9855 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V008015
VERSION     V008015
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9855)
  AUTHORS   De Groote P, Grootjans S, Lippens S, Eichperger C, Leurs K, Kahr I,
            Tanghe G, Bruggeman I, De Schamphelaire W, Urwyler C, Vandenabeele
            P, Haustraete J, Declercq W.
  TITLE     Generation of a new Gateway-compatible inducible lentiviral vector
            platform allowing easy derivation of co-transduced cells
  JOURNAL   BioTechniques 60 (5), 252-259 (2016)
   PUBMED   27177818
REFERENCE   2  (bases 1 to 9855)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2015) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 9855)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9855)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "BioTechniques"; date: "2016"; volume: "60"; issue: "5"; pages:
            "252-259"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (22-DEC-2015) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9855
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    75..90
                     /label="HIV-1 cPPT"
                     /note="HIV-1 cPPT"
     LTR             92..325
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    397..531
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      558..693
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(714..732)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(742..758)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      900..1355
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1381..1485
                     /label="AmpR promoter"
     CDS             1486..2343
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      2517..3105
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    3393..3414
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3429..3459
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    3467..3483
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3491..3507
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        3528..3546
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3574..3800
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3801..3981
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4028..4153
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    4646..4879
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             5064..5108
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             5257..5298
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     protein_bind    5396..5414
                     /label="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5431..5449
                     /gene="tetO"
                     /label="tet operator"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5467..5485
                     /gene="tetO"
                     /label="tet operator"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5503..5521
                     /gene="tetO"
                     /label="tet operator"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5538..5556
                     /gene="tetO"
                     /label="tet operator"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5574..5592
                     /gene="tetO"
                     /label="tet operator"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        5604..5642
                     /label="minimal CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     protein_bind    5707..5831
                     /label="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        5856..5886
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             5940..6596
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     CDS             6941..7243
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(7287..7411)
                     /label="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             7455..7487
                     /label="VSV-G tag"
                     /note="epitope tag from vesicular stomatitis virus G
                     protein"
     promoter        7532..7862
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             7914..8618
                     /label="rtTA3"
                     /note="reverse tetracycline transactivator 3"
     protein_bind    complement(8622..8655)
                     /label="loxP511"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     misc_feature    8678..9260
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             9259..9852
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
ORIGIN
        1 gctaagcaca attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat
       61 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
      121 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
      181 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
      241 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
      301 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
      361 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
      421 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
      481 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
      541 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
      601 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
      661 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag
      721 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
      781 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
      841 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
      901 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
      961 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     1021 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     1081 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     1141 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     1201 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     1261 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     1321 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     1381 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     1441 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     1501 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     1561 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     1621 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     1681 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     1741 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     1801 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     1861 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     1921 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     1981 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     2041 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     2101 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     2161 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     2221 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     2281 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     2341 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     2401 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     2461 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     2521 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     2581 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     2641 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     2701 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     2761 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     2821 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     2881 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     2941 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     3001 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     3061 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     3121 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     3181 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     3241 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     3301 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     3361 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     3421 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     3481 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
     3541 taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt
     3601 cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg
     3661 catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg
     3721 gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt
     3781 gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct
     3841 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     3901 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     3961 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     4021 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     4081 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     4141 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     4201 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     4261 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     4321 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     4381 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     4441 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     4501 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     4561 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     4621 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     4681 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     4741 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     4801 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     4861 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     4921 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     4981 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     5041 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     5101 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     5161 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     5221 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     5281 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
     5341 gattagtgaa cggatctcga cggtatcgat ggtccgaggt tctagacgag tttactccct
     5401 atcagtgata gagaacgatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga
     5461 gtttactccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag
     5521 aacgtatgtc gagtttatcc ctatcagtga tagagaacgt atgtcgagtt tactccctat
     5581 cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg ggaggcctat ataagcagag
     5641 ctcgtttagt gaaccgtcag atcgcaccgg tcactagtcc agtgtggtgg aattctgcag
     5701 atatcaacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat
     5761 atattaaatt agattttgca taaaaaacag actacataat actgtaaaac acaacatatc
     5821 cagtcactat ggcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt
     5881 ataatgtgtg gattttgagt taggatccgg cgagattttc aggagctaag gaagctaaaa
     5941 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
     6001 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
     6061 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
     6121 acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg
     6181 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
     6241 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
     6301 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
     6361 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
     6421 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
     6481 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtctgtgat ggcttccatg
     6541 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa
     6601 gatctggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt
     6661 gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtgtgctat
     6721 gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca aggcatatat
     6781 gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt
     6841 gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa
     6901 atgaacggct cttttgctga cgagaacagg gactggtgaa atgcagttta aggtttacac
     6961 ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac
     7021 gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc
     7081 ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga
     7141 tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga
     7201 aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccgt
     7261 tatacacagc cagtctgcag gtcgaccata gtgactggat atgttgtgtt ttacagtatt
     7321 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
     7381 tttctcgttc agctttcttg tacaaagtgg ttgatatcca gcacagtggc ggccgctcga
     7441 gtctagaggg cccgtacacc gacatcgaga tgaacagact gggcaagacg cgtaccggtt
     7501 agtaatgagt ttggaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     7561 aggctcccca ggcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     7621 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     7681 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     7741 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     7801 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     7861 aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg accatgtcta
     7921 ggctggacaa gagcaaagtc ataaacggag ctctggaatt actcaatggt gtcggtatcg
     7981 aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct accctgtact
     8041 ggcacgtgaa gaacaagcgg gccctgctcg atgccctgcc aatcgagatg ctggacaggc
     8101 atcataccca cttctgcccc ctggaaggcg agtcatggca agactttctg cggaacaacg
     8161 ccaagtcata ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg catctcggca
     8221 cccgcccaac agagaaacag tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc
     8281 aaggcttctc cctggagaac gcactgtacg ctctgtccgc cgtgggccac tttacactgg
     8341 gctgcgtatt ggaggaacag gagcatcaag tagcaaaaga ggaaagagag acacctacca
     8401 ccgattctat gcccccactt ctgagacaag caattgagct gttcgaccgg cagggagccg
     8461 aacctgcctt ccttttcggc ctggaactaa tcatatgtgg cctggagaaa cagctaaagt
     8521 gcgaaagcgg cgggccgacc gacgcccttg acgattttga cttagacatg ctcccagccg
     8581 atgcccttga cgattttgac cttgacatgc tccccgggta aataacttcg tatagtatac
     8641 attatacgaa gttatggatc cgcggccgca aattccgccc ctctccctcc ccccccccta
     8701 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt
     8761 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga
     8821 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg
     8881 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt
     8941 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat
     9001 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg
     9061 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg
     9121 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt
     9181 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa
     9241 cacgataata ccatggccac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     9301 gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     9361 accgtcgacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     9421 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     9481 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg ctcgcgcatg
     9541 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     9601 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     9661 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgctggg
     9721 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     9781 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     9841 aagcccggtg cctga
//
