LOCUS       V008024                 9809 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V008024
VERSION     V008024
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9809)
  AUTHORS   De Groote P, Grootjans S, Lippens S, Eichperger C, Leurs K, Kahr I,
            Tanghe G, Bruggeman I, De Schamphelaire W, Urwyler C, Vandenabeele
            P, Haustraete J, Declercq W.
  TITLE     Generation of a new Gateway-compatible inducible lentiviral vector
            platform allowing easy derivation of co-transduced cells
  JOURNAL   BioTechniques 60 (5), 252-259 (2016)
   PUBMED   27177818
REFERENCE   2  (bases 1 to 9809)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2015) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 9809)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9809)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "BioTechniques"; date: "2016"; volume: "60"; issue: "5"; pages:
            "252-259"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (22-DEC-2015) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9809
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    35..159
                     /label="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        184..214
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             268..924
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     CDS             1269..1571
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(1615..1739)
                     /label="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1771..3198
                     /label="tdTomato"
                     /note="tandem dimeric (pseudo-monomeric) derivative of
                     DsRed (Shaner et al., 2004)"
     CDS             3217..3258
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label="V5 tag"
                     /translation="GKPIPNPLLGLDST"
     promoter        3300..3630
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        3678..3725
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             3744..4139
                     /label="BSD"
                     /note="blasticidin S deaminase"
     misc_feature    4212..4227
                     /label="HIV-1 cPPT"
                     /note="HIV-1 cPPT"
     LTR             4229..4462
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4534..4668
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      4695..4830
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(4851..4869)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4879..4895)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      5037..5492
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5518..5622
                     /label="AmpR promoter"
     CDS             5623..6480
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6654..7242
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    7530..7551
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7566..7596
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    7604..7620
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7628..7644
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        7665..7683
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7711..7937
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7938..8118
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8165..8290
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    8783..9016
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             9201..9245
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             9394..9435
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     protein_bind    9533..9551
                     /label="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9568..9586
                     /gene="tetO"
                     /label="tet operator"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9604..9622
                     /gene="tetO"
                     /label="tet operator"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9640..9658
                     /gene="tetO"
                     /label="tet operator"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9675..9693
                     /gene="tetO"
                     /label="tet operator"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9711..9729
                     /gene="tetO"
                     /label="tet operator"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        9741..9779
                     /label="minimal CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
ORIGIN
        1 actagtccag tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagctgaacg
       61 agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata aaaaacagac
      121 tacataatac tgtaaaacac aacatatcca gtcactatgg cggccgcatt aggcacccca
      181 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccggcg
      241 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
      301 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
      361 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
      421 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
      481 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
      541 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
      601 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
      661 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
      721 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
      781 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
      841 catcatgccg tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
      901 tgcgatgagt ggcagggcgg ggcgtaaaga tctggatccg gcttactaaa agccagataa
      961 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc
     1021 cgaagtatgt caaaaagagg tgtgctatga agcagcgtat tacagtgaca gttgacagcg
     1081 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac
     1141 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg
     1201 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacaggga
     1261 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
     1321 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca
     1381 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg
     1441 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg
     1501 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
     1561 tctggggaat ataaatgtca ggctccgtta tacacagcca gtctgcaggt cgaccatagt
     1621 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
     1681 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt
     1741 gatatccagc acagtggcgg ccgctcgagt atggtgagca agggcgagga ggtcatcaaa
     1801 gagttcatgc gcttcaaggt gcgcatggag ggctccatga acggccacga gttcgagatc
     1861 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     1921 aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcat gtacggctcc
     1981 aaggcgtacg tgaagcaccc cgccgacatc cccgattaca agaagctgtc cttccccgag
     2041 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gtctggtgac cgtgacccag
     2101 gactcctccc tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg caccaacttc
     2161 ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc caccgagcgc
     2221 ctgtaccccc gcgacggcgt gctgaagggc gagatccacc aggccctgaa gctgaaggac
     2281 ggcggccact acctggtgga gttcaagacc atctacatgg ccaagaagcc cgtgcaactg
     2341 cccggctact actacgtgga caccaagctg gacatcacct cccacaacga ggactacacc
     2401 atcgtggaac agtacgagcg ctccgagggc cgccaccacc tgttcctggg gcatggcacc
     2461 ggcagcaccg gcagcggcag ctccggcacc gcctcctccg aggacaacaa catggccgtc
     2521 atcaaagagt tcatgcgctt caaggtgcgc atggagggct ccatgaacgg ccacgagttc
     2581 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     2641 gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttcatgtac
     2701 ggctccaagg cgtacgtgaa gcaccccgcc gacatccccg attacaagaa gctgtccttc
     2761 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggtct ggtgaccgtg
     2821 acccaggact cctccctgca ggacggcacg ctgatctaca aggtgaagat gcgcggcacc
     2881 aacttccccc ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctccacc
     2941 gagcgcctgt acccccgcga cggcgtgctg aagggcgaga tccaccaggc cctgaagctg
     3001 aaggacggcg gccactacct ggtggagttc aagaccatct acatggccaa gaagcccgtg
     3061 caactgcccg gctactacta cgtggacacc aagctggaca tcacctccca caacgaggac
     3121 tacaccatcg tggaacagta cgagcgctcc gagggccgcc accacctgtt cctgtacggc
     3181 atggacgagc tgtacaagga gggcccgcgg ttcgaaggta agcctatccc taaccctctc
     3241 ctcggtctcg attctacgcg taccggttag taatgagttt ggaattaatt ctgtggaatg
     3301 tgtgtcagtt agggtgtgga aagtccccag gctccccagg caggcagaag tatgcaaagc
     3361 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     3421 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     3481 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     3541 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     3601 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     3661 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat
     3721 acgacaaggt gaggaactaa accatggcca agcctttgtc tcaagaagaa tccaccctca
     3781 ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca
     3841 gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg
     3901 ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc
     3961 tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt
     4021 gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg
     4081 gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct
     4141 aagcacaatt cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     4201 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     4261 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     4321 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     4381 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     4441 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     4501 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     4561 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     4621 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     4681 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     4741 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     4801 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
     4861 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     4921 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     4981 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     5041 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     5101 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     5161 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     5221 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     5281 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     5341 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     5401 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     5461 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     5521 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     5581 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     5641 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     5701 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     5761 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     5821 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     5881 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     5941 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     6001 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     6061 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     6121 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     6181 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     6241 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     6301 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     6361 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     6421 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     6481 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     6541 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     6601 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     6661 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     6721 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     6781 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     6841 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     6901 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     6961 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     7021 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     7081 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     7141 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     7201 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     7261 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     7321 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     7381 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     7441 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     7501 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     7561 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     7621 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
     7681 agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt
     7741 gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat
     7801 gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc
     7861 tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc
     7921 tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     7981 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     8041 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     8101 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
     8161 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
     8221 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
     8281 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
     8341 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
     8401 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
     8461 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
     8521 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
     8581 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
     8641 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
     8701 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
     8761 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
     8821 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
     8881 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
     8941 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
     9001 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
     9061 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
     9121 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
     9181 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
     9241 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
     9301 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
     9361 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg
     9421 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat
     9481 tagtgaacgg atctcgacgg tatcgatggt ccgaggttct agacgagttt actccctatc
     9541 agtgatagag aacgatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt
     9601 tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac
     9661 gtatgtcgag tttatcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag
     9721 tgatagagaa cgtatgtcga ggtaggcgtg tacggtggga ggcctatata agcagagctc
     9781 gtttagtgaa ccgtcagatc gcaccggtc
//
