LOCUS       V014182                10156 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014182
VERSION     V014182
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10156)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(699..3731)
                     /label="lacZ"
                     /note="beta-galactosidase"
     CDS             4140..4787
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             7298..8077
                     /gene="ant1"
                     /label="Spectinomycin 9-adenylyltransferase"
                     /note="Spectinomycin 9-adenylyltransferase from
                     Staphylococcus aureus (strain N315). Accession#: P0A0D1"
     rep_origin      complement(8327..8915)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9089..9946)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9947..10051)
                     /label="AmpR promoter"
ORIGIN
        1 aacaaaattc tccagtcttc acatcggttt gaaaggagga agcggaagaa tgaagtaaga
       61 gggatttttg actccgaagt aagtcttcaa aaaatcaaat aaggagtgtc aagaatgttt
      121 gcaaaacgat tcaaaacctc tttactgccg ttattcgctg gatttttatt gctgtttcat
      181 ttggttctgg caggaccggc ggctgcgagt gctgaaacgg cgaacaaatc gaatgagctt
      241 acagcaccgt cgatcaaaag cggaaccatt cttcatgcat ggaattggtc gttcaatacg
      301 ttaaaacaca atatgaagga tattcatgat gcaggatata cagccattca gacatctccg
      361 attaaccaag taaaggaagg gaatcaagga gataaaagca tgtcgaactg gtactggctg
      421 tatcagccga catcgtatca aattggcaac cgttacttag gtactgaaca agaatttaaa
      481 gaaatgtgtg cagccgctga agaatatggc ataaaggtca ttgttgacgc ggtcatcaat
      541 cataccacca gtgattatgc cgcgatttcc aatgaggtta agagtattcc aaactggaca
      601 catggaaaca cacaaattaa aaactggtct gatcgatagt acataatgga tttccttacg
      661 cgaaatacgg gcagacatgg cctgcccggt tattattatt tttgacacca gaccaactgg
      721 taatggtagc gaccggcgct cagctgaaat tccgccgata ctgacgggct ccaggagtcg
      781 tcgccaccaa tccccatatg gaaaccgtcg atattcagcc atgtgccttc ttccgcgtgc
      841 agcagatggc gatggctggt ttccatcagt tgctgttgac tgtagcggct gatgttgaac
      901 tggaagtcgc cgcgccactg gtgtgggcca taattcaatt cgcgcgtccc gcagcgcaga
      961 ccgttttcgc tcgggaagac gtacggggta tacatgtctg acaatggcag atcccagcgg
     1021 tcaaaacagg cggcagtaag gcggtcggga tagttttctt gcggccctaa tccgagccag
     1081 tttacccgct ctgctacctg cgccagctgg cagttcaggc caatccgcgc cggatgcggt
     1141 gtatcgctcg ccacttcaac atcaacggta atcgccattt gaccactacc atcaatccgg
     1201 taggttttcc ggctgataaa taaggttttc ccctgatgct gccacgcgtg agcggtcgta
     1261 atcagcaccg catcagcaag tgtatctgcc gtgcactgca acaacgctgc ttcggcctgg
     1321 taatggcccg ccgccttcca gcgttcgacc caggcgttag ggtcaatgcg ggtcgcttca
     1381 cttacgccaa tgtcgttatc cagcggtgca cgggtgaact gatcgcgcag cggcgtcagc
     1441 agttgttttt tatcgccaat ccacatctgt gaaagaaagc ctgactggcg gttaaattgc
     1501 caacgcttat tacccagctc gatgcaaaaa tccatttcgc tggtggtcag atgcgggatg
     1561 gcgtgggacg cggcggggag cgtcacactg aggttttccg ccagacgcca ctgctgccag
     1621 gcgctgatgt gcccggcttc tgaccatgcg gtcgcgttcg gttgcactac gcgtactgtg
     1681 agccagagtt gcccggcgct ctccggctgc ggtagttcag gcagttcaat caactgttta
     1741 ccttgtggag cgacatccag aggcacttca ccgcttgcca gcggcttacc atccagcgcc
     1801 accatccagt gcaggagctc gttatcgcta tgacggaaca ggtattcgct ggtcacttcg
     1861 atggtttgcc cggataaacg gaactggaaa aactgctgct ggtgttttgc ttccgtcagc
     1921 gctggatgcg gcgtgcggtc ggcaaagacc agaccgttca tacagaactg gcgatcgttc
     1981 ggcgtatcgc caaaatcacc gccgtaagcc gaccacgggt tgccgttttc atcatattta
     2041 atcagcgact gatccaccca gtcccagacg aagccgccct gtaaacgggg atactgacga
     2101 aacgcctgcc agtatttagc gaaaccgcca agactgttac ccatcgcgtg ggcgtattcg
     2161 caaaggatca gcgggcgcgt ctctccaggt agcgaaagcc attttttgat ggaccatttc
     2221 ggcacagccg ggaagggctg gtcttcatcc acgcgcgcgt acatcgggca aataatatcg
     2281 gtggccgtgg tgtcggctcc gccgccttca tactgcaccg ggcgggaagg atcgacagat
     2341 ttgatccagc gatacagcgc gtcgtgatta gcgccgtggc ctgattcatt ccccagcgac
     2401 cagatgatca cactcgggtg attacgatcg cgctgcacca ttcgcgttac gcgttcgctc
     2461 atcgccggta gccagcgcgg atcatcggtc agacgattca ttggcaccat gccgtgggtt
     2521 tcaatattgg cttcatccac cacatacagg ccgtagcggt cgcacagcgt gtaccacagc
     2581 ggatggttcg gataatgcga acagcgcacg gcgttaaagt tgttctgctt catcagcagg
     2641 atatcctgca ccatcgtctg ctcatccatg acctgaccat gcagaggatg atgctcgtga
     2701 cggttaacgc ctcgaatcag caacggcttg ccgttcagca gcagcagacc attttcaatc
     2761 cgcacctcgc ggaaaccgac atcgcaggct tctgcttcaa tcagcgtgcc gtcggcggtg
     2821 tgcagttcaa ccaccgcacg atagagattc gggatttcgg cgctccacag tttcgggttt
     2881 tcgacgttca gacgtagtgt gacgcgatcg gcataaccac cacgctcatc gataatttca
     2941 ccgccgaaag gcgcggtgcc gctggcgacc tgcgtttcac cctgccataa agaaactgtt
     3001 acccgtaggt agtcacgcaa ctcgccgcac atctgaactt cagcctccag tacagcgcgg
     3061 ctgaaatcat cattaaagcg agtggcaaca tggaaatcgc tgatttgtgt agtcggttta
     3121 tgcagcaacg agacgtcacg gaaaatgccg ctcatccgcc acatatcctg atcttccaga
     3181 taactgccgt cactccaacg cagcaccatc accgcgaggc ggttttctcc ggcgcgtaaa
     3241 aatgcgctca ggtcaaattc agacggcaaa cgactgtcct ggccgtaacc gacccagcgc
     3301 ccgttgcacc acagatgaaa cgccgagtta acgccatcaa aaataattcg cgtctggcct
     3361 tcctgtagcc agctttcatc aacattaaat gtgagcgagt aacaacccgt cggattctcc
     3421 gtgggaacaa acggcggatt gaccgtaatg ggataggtta cgttggtgta gatgggcgca
     3481 tcgtaaccgt gcatctgcca gtttgagggg acgacgacag tatcggcctc aggaagatcg
     3541 cactccagcc agctttccgg caccgcttct ggtgccggaa accaggcaaa gcgccattcg
     3601 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     3661 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     3721 cggtcgaccc gtaatcttac gtcagtaact tccacagtag ttcaccacct tttccctata
     3781 taaaagcatt agtgtatcaa caagctgggg atcctagaag cttatcgaat tctcatgttt
     3841 gacagcttat catcggcaat agttaccctt attatcaaga taagaaagaa aaggattttt
     3901 cgctacgctc aaatccttta aaaaaacaca aaagaccaca ttttttaatg tggtctttat
     3961 tcttcaacta aagcacccat tagttcaaca aacgaaaatt ggataaagtg ggatattttt
     4021 aaaatatata tttatgttac agtaatattg acttttaaaa aaggattgat tctaatgaag
     4081 aaagcagaca agtaagcctc ctaaattcac tttagataaa aatttaggag gcatatcaaa
     4141 tgaactttaa taaaattgat ttagacaatt ggaagagaaa agagatattt aatcattatt
     4201 tgaaccaaca aacgactttt agtataacca cagaaattga tattagtgtt ttataccgaa
     4261 acataaaaca agaaggatat aaattttacc ctgcatttat tttcttagtg acaagggtga
     4321 taaactcaaa tacagctttt agaactggtt acaatagcga cggagagtta ggttattggg
     4381 ataagttaga gccactttat acaatttttg atggtgtatc taaaacattc tctggtattt
     4441 ggactcctgt aaagaatgac ttcaaagagt tttatgattt atacctttct gatgtagaga
     4501 aatataatgg ttcggggaaa ttgtttccca aaacacctat acctgaaaat gctttttctc
     4561 tttctattat tccatggact tcatttactg ggtttaactt aaatatcaat aataatagta
     4621 attaccttct acccattatt acagcaggaa aattcattaa taaaggtaat tcaatatatt
     4681 taccgctatc tttacaggta catcattctg tttgtgatgg ttatcatgca ggattgttta
     4741 tgaactctat tcaggaattg tcagataggc ctaatgactg gcttttataa tatgagataa
     4801 tgccgactgt actttttaca gtcggttttc taatgtcact aacctgcccc gttagttgaa
     4861 gaaggttttt atattacagc tccagatcct ctacgccgga cgcatcgtgg ccggcatcac
     4921 cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg
     4981 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt
     5041 ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct
     5101 caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg
     5161 tcgacatgga tgagcgatga tgatatccgt ttaggctggg cggtgatagc ttctcgttca
     5221 ggcagtacgc ctcttttctt ttccagacct gagggaggcg gaaatggtgt gaggttcccg
     5281 gggaaaagcc aaataggcga tcgcgggagt gctttatttg aagatcaggc tatcactgcg
     5341 gtcaatagat ttcacaatgt gatggctgga cagcctgagg aactctcgaa cccgaatgga
     5401 aacaaccaga tatttatgaa tcagcgcggc tcacatggcg ttgtgctggc aaatgcaggt
     5461 tcatcctctg tctctatcaa tacggcaaca aaattgcctg atggcaggta tgacaataaa
     5521 gctggagcgg gttcatttca agtgaacgat ggtaaactga caggcacgat caatgccagg
     5581 tctgtagctg tgctttatcc tgatgatatt gcaaaagcgc ctcatgtttt ccttgagaat
     5641 tacaaaacag gtgtaacaca ttctttcaat gatcaactga cgattacctt gcgtgcagat
     5701 gcgaatacaa caaaagccgt ttatcaaatc aataatggac cagacgacag gcgtttaagg
     5761 atggagatca attcacaatc ggaaaaggag atccaatttg gcaaaacata caccatcatg
     5821 ttaaaaggaa cgaacagtga tggtgtaacg aggaccgaga aatacagttt tgttaaaaga
     5881 gatccagcgt cggccaaaac catcggctat caaaatccga atcattggag ccaggtaaat
     5941 gcttatatct ataaacatga tgggagccga gtaattgaat tgaccggatc ttggcctgga
     6001 aaaccaatga ctaaaaatgc agacggaatt tacacgctga cgctgcctgc ggacacggat
     6061 acaaccaacg caaaagtgat ttttaataat ggcagcgccc aagtgcccgg tcagaatcag
     6121 cctggctttg attacgtgct aaatggttta tataatgact cgggcttaag cggttctctt
     6181 ccccattgag ggcaaggcta gacgggactt accgaaagaa accatcaatg atggtttctt
     6241 ttttgttcat aaatcagaca aaacttttct cttgcaaaag tttgtgaagt gttgcacaat
     6301 ataaatgtga aatacttcac aaacaaaaag acatcaaaga gaaacatacc ctgcaaggat
     6361 gctgatattg tctgcatttg cgccggagca aaccaaaaac ctggtgagac acgccttgaa
     6421 ttagtagaaa agaacttgaa gattttcaaa ggcatcgtta gtgaagtcat ggcgagcgga
     6481 tttgacggca ttttcttagt cgcgacgcga ggctggatgg ccttccccat tatgattctt
     6541 ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat
     6601 gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc
     6661 actggaccgc tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg
     6721 gcatggattg taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca
     6781 tggagccggg ccacctactg aagtggattt ctttaagagc tcctttaact tcctcaccag
     6841 tagttgtatc ggtaccataa gtagaagcag caacccaagt agctttacca gcatccggtt
     6901 caaccagcat agtaagaatc ttactggaca tcggcagttc ttcgaacagt gcgccaacta
     6961 ccagctcttt ctgcagttca ttcagggcac cggagaacct gcgtgcaatc catcttgttc
     7021 aatcatgcga aacgatcctc atcctgtctc ttgatccatg gattacgcgt taacccgggc
     7081 ccgcggatgc atatgatcag atcttaaggc ctaggtctag aggatcgatc tgtataataa
     7141 agaataatta ttaatctgta gacaaattgt gaaaggatgt acttaaacgc taacggtcag
     7201 ctttattgaa cagtaattta agtatatgtc caatctaggg taagtaaatt gagtatcaat
     7261 ataaacttta tatgaacata atcaacgagg tgaaatcatg agcaatttga ttaacggaaa
     7321 aataccaaat caagcgattc aaacattaaa aatcgtaaaa gatttatttg gaagttcaat
     7381 agttggagta tatctatttg gttcagcagt aaatggtggt ttacgcatta acagcgatgt
     7441 agatgttcta gtcgtcgtga atcatagttt acctcaatta actcgaaaaa aactaacaga
     7501 aagactaatg actatatcag gaaagattgg aaatacggat tctgttagac cacttgaagt
     7561 tacggttata aataggagtg aagttgtccc ttggcaatat cctccaaaaa gagaatttat
     7621 atacggtgag tggctcaggg gtgaatttga gaatggacaa attcaggaac caagctatga
     7681 tcctgatttg gctattgttt tagcacaagc aagaaagaat agtatttctc tatttggtcc
     7741 tgattcttca agtatacttg tctccgtacc tttgacagat attcgaagag caattaagga
     7801 ttctttgcca gaactaattg aggggataaa aggtgatgag cgtaatgtaa ttttaaccct
     7861 agctcgaatg tggcaaacag tgactactgg tgaaattacc tcgaaagatg tcgctgcaga
     7921 atgggctata cctcttttac ctaaagagca tgtaacttta ctggatatag ctagaaaagg
     7981 ctatcgggga gagtgtgatg ataagtggga aggactatat tcaaaggtga aagcactcgt
     8041 taagtatatg aaaaattcta tagaaacttc tctcaattag gctaatttta ttgcaataac
     8101 aggtgcttac ttttctggag ttctttagca aattttttta ttagctgaac ttagtattag
     8161 tggccatact cctccaatcc aaagctattt agaaagatta ctatatcctc aaacaggcgg
     8221 taaccggcct cttcatcggg aatgcgcgcg accttcagca tcgccggcat gtccccctgg
     8281 cggacgggaa gtatccagct cgaggtcggg ccgcgttgct ggcgtttttc cataggctcc
     8341 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     8401 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     8461 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     8521 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     8581 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     8641 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     8701 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     8761 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     8821 ttgatagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     8881 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     8941 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     9001 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     9061 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     9121 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     9181 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     9241 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     9301 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     9361 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac
     9421 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     9481 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     9541 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     9601 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     9661 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc
     9721 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     9781 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     9841 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     9901 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     9961 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    10021 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
    10081 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
    10141 ttcgtcttca agaatt
//
