LOCUS       62056_8385        8750 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8750)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8750
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    465..481
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             766..1845
                     /codon_start=1
                     /label=lacI
                     /note="lac repressor"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKTAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     misc_feature    2019..2159
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2345..2933)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3107..3964)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3965..4069)
                     /label=AmpR promoter
     CDS             4588..5589
                     /codon_start=1
                     /label=repB
                     /note="RepB replication protein"
                     /translation="MGFSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
                     FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
                     QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
                     GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
                     VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
                     YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
ORIGIN
        1 cgggaaaaac gggaagtagg ctcccatgat aaaaaagtaa aagaaaaaga ataaaccgaa
       61 catccaaaag tttgtgtttt ttaaatagta cataatggat ttccttacgc gaaatacggg
      121 cagacatggc ctgcccggtt attattattt ttgacaccag accaactggt aatggtagcg
      181 accggcgctc agctggaatt ctacacagcc cagtccagac tattcggcac tgaaattatg
      241 ggtgaagtgg tcaagacctc actaggcacc ttaaaaatag cgcaccctga agaagattta
      301 tttgaggtag cccttgccta cctagcttcc aagaaagata tcctaacagc acaagagcgg
      361 aaagatgttt tgttctacat ccagaacaac ctctgctaaa attcctgaaa aattttgcaa
      421 aaagttgttg actttatcta caaggtgtgg cataatgtgt ggaattgtga gcggataaca
      481 attaagctta aggaggtgat ctagagtcga cctgcaggca tgcaagctaa ttcggtggaa
      541 acgaggtcat catttccttc cgaaaaaacg gttgcattta aatcttacat atgtaatact
      601 ttcaaagact acatttgtaa gatttgatgt ttgagtcggc tgaaagatcg tacgtaccaa
      661 ttattgtttc gtgattgttc aagccataac actgtaggga tagtggaaag agtgcttcat
      721 ctggttacga tcaatcaaat attcaaacgg agggagacga ttttgatgaa accagtaacg
      781 ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac
      841 caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg
      901 aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc
      961 gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct
     1021 cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa
     1081 gcctgtaaaa cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac
     1141 tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg
     1201 ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac
     1261 ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta
     1321 gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc
     1381 actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt
     1441 tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc
     1501 aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt
     1561 gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg
     1621 ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg
     1681 caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa
     1741 agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     1801 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     1861 taatgtgagt taggatcctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     1921 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     1981 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
     2041 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag
     2101 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     2161 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     2221 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     2281 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     2341 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     2401 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     2461 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     2521 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     2581 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     2641 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     2701 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     2761 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     2821 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     2881 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     2941 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     3001 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     3061 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     3121 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     3181 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     3241 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     3301 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     3361 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg
     3421 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     3481 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     3541 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     3601 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     3661 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     3721 cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     3781 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     3841 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     3901 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     3961 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     4021 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     4081 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     4141 ggcgtatcac gaggcccttt cgtcttcaag aattccttaa ggaacgtaca gacggcttaa
     4201 aagcctttaa aaacgttttt aaggggtttg tagacaaggt aaaggataaa acagcacaat
     4261 tccaagaaaa acacgattta gaacctaaaa agaacgaatt tgaactaact cataaccgag
     4321 aggtaaaaaa agaacgaagt cgagatcagg gaatgagttt ataaaataaa aaaagcacct
     4381 gaaaaggtgt ctttttttga tggttttgaa cttgttcttt cttatcttga tacatataga
     4441 aataacgtca tttttatttt agttgctgaa aggtgcgttg aagtgttggt atgtatgtgt
     4501 tttaaagtat tgaaaaccct taaaattggt tgcacagaaa aaccccatct gttaaagtta
     4561 taagtgacta aacaaataac taaatagatg gggttttctt ttaatattat gtgtcctaat
     4621 agtagcattt attcagatga aaaatcaagg gttttagtgg acaagacaaa aagtggaaaa
     4681 gtgagaccat ggagagaaaa gaaaatcgct aatgttgatt actttgaact tctgcatatt
     4741 cttgaattta aaaaggctga aagagtaaaa gattgtgctg aaatattaga gtataaacaa
     4801 aatcgtgaaa caggcgaaag aaagttgtat cgagtgtggt tttgtaaatc caggctttgt
     4861 ccaatgtgca actggaggag agcaatgaaa catggcattc agtcacaaaa ggttgttgct
     4921 gaagttatta aacaaaagcc aacagttcgt tggttgtttc tcacattaac agttaaaaat
     4981 gtttatgatg gcgaagaatt aaataagagt ttgtcagata tggctcaagg atttcgccga
     5041 atgatgcaat ataaaaaaat taataaaaat cttgttggtt ttatgcgtgc aacggaagtg
     5101 acaataaata ataaagataa ttcttataat cagcacatgc atgtattggt atgtgtggaa
     5161 ccaacttatt ttaagaatac agaaaactac gtgaatcaaa aacaatggat tcaattttgg
     5221 aaaaaggcaa tgaaattaga ctatgatcca aatgtaaaag ttcaaatgat tcgaccgaaa
     5281 aataaatata aatcggatat acaatcggca attgacgaaa ctgcaaaata tcctgtaaag
     5341 gatacggatt ttatgaccga tgatgaagaa aagaatttga aacgtttgtc tgatttggag
     5401 gaaggtttac accgtaaaag gttaatctcc tatggtggtt tgttaaaaga aatacataaa
     5461 aaattaaacc ttgatgacac agaagaaggc gatttgattc atacagatga tgacgaaaaa
     5521 gccgatgaag atggattttc tattattgca atgtggaatt gggaacggaa aaattatttt
     5581 attaaagagt agttcaacaa acgggccagt ttgttgaaga ttagatgcta taattgttat
     5641 taaaaggatt gaaggatgct taggaagacg agttattaat agctgaataa gaacggtgct
     5701 ctccaaatat tcttatttag aaaagcaaat ctaaaattat ctgaaaaggg aatgagaata
     5761 gtgaatggac caataataat gactagagaa gaaagaatga agattgttca tgaaattaag
     5821 gaacgaatat tggataaata tggggatgat gttaaggcta ttggtgttta tggctctctt
     5881 ggtcgtcaga ctgatgggcc ctattcggat attgagatga tgtgtgtcat gtcaacagag
     5941 gaagcagagt tcagccatga atggacaacc ggtgagtgga aggtggaagt gaattttgat
     6001 agcgaagaga ttctactaga ttatgcatct caggtggaat cagattggcc gcttacacat
     6061 ggtcaatttt tctctatttt gccgatttat gattcaggtg gatacttaga gaaagtgtat
     6121 caaactgcta aatcggtaga agcccaaacg ttccacgatg cgatttgtgc ccttatcgta
     6181 gaagagctgt ttgaatatgc aggcaaatgg cgtaatattc gtgtgcaagg accgacaaca
     6241 tttctaccat ccttgactgt acaggtagca atggcaggtg ccatgttgat tggtctgcat
     6301 catcgcatct gttatacgac gagcgcttcg gtcttaactg aagcagttaa gcaatcagat
     6361 cttccttcag gttatgacca tctgtgccag ttcgtaatgt ctggtcaact ttccgactct
     6421 gagaaacttc tggaatcgct agagaatttc tggaatggga ttcaggagtg gacagaacga
     6481 cacggatata tagtggatgt gtcaaaacgc ataccatttt gaacgatgac ctctaataat
     6541 tgttaatcat gttggttacg tatttattaa cttctcctag tattagtaat tatcatggct
     6601 gtcatggcgc attaacggaa taaagggtgt gcttaaatcg ggccattttg cgtaataaga
     6661 aaaaggatta attatgagcg aattgaatta ataataaggt aatagattta cattagaaaa
     6721 tgaaagggga ttttatgcgt gagaatgtta cagtctatcc cggcattgcc agtcggggat
     6781 attaaaaaga gtataggttt ttattgcgat aaactaggtt tcactttggt tcaccatgaa
     6841 gatggattcg cagttctaat gtgtaatgag gttcggattc atctatggga ggcaagtgat
     6901 gaaggctggc gctctcgtag taatgattca ccggtttgta caggtgcgga gtcgtttatt
     6961 gctggtactg ctagttgccg cattgaagta gagggaattg atgaattata tcaacatatt
     7021 aagcctttgg gcattttgca ccccaataca tcattaaaag atcagtggtg ggatgaacga
     7081 gactttgcag taattgatcc cgacaacaat ttgattagct tttttcaaca aataaaaagc
     7141 taaaatctat tattaatctg ttcagcaatc gggcgcgatt gctgaataaa agatacgaga
     7201 gacctctctt gtatcttttt tattttgagt ggttttgtcc gttacactag aaaaccgaaa
     7261 gacaataaaa attttattct tgctgagtct ggctttcggt aagctagaca aaacggacaa
     7321 aataaaaatt ggcaagggtt taaaggtgga gattttttga gtgatcttct caaaaaatac
     7381 tacctgtccc ttgctgattt ttaaacgagc acgagagcaa aacccccctt tgctgaggtg
     7441 gcgaacgatg agccaataat acgtacagac ataatagtgc cagccagcag cagggcgttt
     7501 ttcccaccga tgcgattaat gatcagtggc gcaaagaaca taatcgaggc gttaagtaat
     7561 tcgcccattg tcgttacgta gccaaatacc cgcgtaccct gttcaccggt agcaaagaac
     7621 gaagtaaaga aattagcaaa ctgttggtca aaaacatcgt aggtgcagga aacgccaata
     7681 acatacagtg acaaaaacca cagttttggc tgtctgaaca gttccagtgc cagcttaagg
     7741 ctaaatgccg aatggttggc acctaccgca ttggcaaccg tggcagaaga gggcgcatcc
     7801 gttttggcga aaaagagtaa aacggcgagg atgagtgcac agccagagcc cagccagaaa
     7861 acaaactgat tattgatggt gaacatgatg ccgacaatcg aggcacacag cgcccagcca
     7921 acacagccaa acatccgcgc gcgaccaaat tcgaaattac tgcgacggct gactttctca
     7981 ataaatgcct ctactgctgg cgcaccggcg ttaaaacaaa agcctagata aataccacca
     8041 acaatcgatc ctactaaaat gttgtattgt aacagtggcc cgaagataaa aataaagaac
     8101 ggcgcaaaca tcactaacat gccggtaata atccacagca ggtatttgcg cagcccgagt
     8161 ttgtcagaaa gcagaccaaa cagcggttgg aataatagcg agaacagaga aatagcggca
     8221 aaaataatac ccgtatcact tttgctgata tggttgatgt catgtagcca aatcgggaaa
     8281 aacgggaagt aggctcccat gataaaaaag taaaagaaaa agaataaacc gaacatccaa
     8341 aagtttgtgt tttttaaata gtacataatg gatttcctta cgcgaaatac gggcagacat
     8401 ggcctgcccg gttattatta tttttgacac cagaccaact ggtaatggta gcgaccggcg
     8461 ctcagctgga attctacaca gcccagtcca gactattcgg cactgaaatt atgggtgaag
     8521 tggtcaagac ctcactaggc accttaaaaa tagcgcaccc tgaagaagat ttatttgagg
     8581 tagcccttgc ctacctagct tccaagaaag atatcctaac agcacaagag cggaaagatg
     8641 ttttgttcta catccagaac aacctctgct aaaattcctg aaaaattttg caaaaagttg
     8701 ttgactttat ctacaaggtg tggcataatg tgtggaattg tgagcggata
//
