LOCUS       40924_14520        8285 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast two-hybrid vector pDG1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8285)
  AUTHORS   Guo D, Hazbun TR, Xu XJ, Ng SL, Fields S, Kuo MH.
  TITLE     A tethered catalysis, two-hybrid system to identify protein-protein 
            interactions requiring post-translational modifications
  JOURNAL   Nat. Biotechnol. 22 (7), 888-892 (2004)
  PUBMED    15208639
REFERENCE   2  (bases 1 to 8285)
  AUTHORS   Kuo M-H., Guo D.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2004) Biochem. Mol. Biol., Michigan State 
            University, 309 BCH Building, East Lansing, MI 48824, USA
REFERENCE   3  (bases 1 to 8285)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8285)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Biotechnol."; date: "2004"; volume: "22"; issue: "7"; pages: 
            "888-892"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-JUN-2004) Biochem. Mol. Biol., Michigan State University, 309 
            BCH Building, East Lansing, MI 48824, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8285
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             434..874
                     /codon_start=1
                     /label=GAL4 DNA binding domain
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     misc_feature    953..1129
                     /label=histone H3
                     /note="histone H3"
     CDS             1193..1204
                     /codon_start=1
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
     misc_feature    1208..1912
                     /label=Gcn5 catalytic domain
                     /note="Gcn5 catalytic domain"
     CDS             1946..2035
                     /codon_start=1
                     /label=3xHA
                     /note="three tandem HA epitope tags"
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      2474..2661
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     misc_feature    complement(3493..4655)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN4 centromere fused to the
                     autonomously replicating sequence ARS1/ARS416"
     promoter        complement(5169..5270)
                     /label=TRP1 promoter
     promoter        5376..5480
                     /label=AmpR promoter
     CDS             5481..6338
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6512..7100
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gtggtacata acgaactaat actgtagccc tagacttgat agccatcatc atatcgaagt
       61 ttcactaccc tttttccatt tgccatctat tgaagtaata ataggcgcat gcaacttctt
      121 ttcttttttt ttcttttctc tctcccccgt tgttgtctca ccatatccgc aatgacaaaa
      181 aaatgatgga agacactaaa ggaaaaaatt aacgacaaag acagcaccaa cagatgtcgt
      241 tgttccagag ctgatgaggg gtatctcgaa gcacacgaaa ctttttcctt ccttcattga
      301 cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt
      361 ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa gcttgaagca
      421 agcctcctga aagatgaagc tactgtcttc tatcgaacaa gcatgcgata tttgccgact
      481 taaaaagctc aagtgctcca aagaaaaacc gaagtgcgcc aagtgtctga agaacaactg
      541 ggagtgtcgc tactctccca aaaccaaaag gtctccgctg actagggcac atctgacaga
      601 agtggaatca aggctagaaa gactggaaca gctatttcta ctgatttttc ctcgagaaga
      661 ccttgacatg attttgaaaa tggattcttt acaggatata aaagcattgt taacaggatt
      721 atttgtacaa gataatgtga ataaagatgc cgtcacagat agattggctt cagtggagac
      781 tgatatgcct ctaacattga gacagcatag aataagtgcg acatcatcat cggaagagag
      841 tagtaacaaa ggtcaaagac agttgactgt atcgaactat ctattcgatg atgaagatac
      901 cccaccaaac ccaaaaaaag agatcgaatt ccagctgacc accatgttta tggccagaac
      961 aaagcaaaca gcaagaaagt ccactggtgg taaggcccca agaaagcaat tagcttctaa
     1021 ggctgccaga aaatccgccc catctaccgg tggtgttaag aagcctcaca gatataagcc
     1081 aggtactgtt gctttgagag aaatcagaag attccaaaaa tctactgaac ccgggtcccc
     1141 tatactaggt tattggaaag gtcgacgcga ccatcctcca aaatcggatc tgatcgaagg
     1201 tcgtggagat cccgaagtta aacgggtaaa attagaaaac aacgttgaag aaatacaacc
     1261 tgagcaggct gagaccaata aacaagaggg caccgataaa gagaataaag gaaagttcga
     1321 gaaagaaact gagagaatag gaggatctga agtggttaca gatgtggaaa aaggaattgt
     1381 caaatttgaa tttgatggtg ttgaatacac attcaaagag agacccagtg tcgtagagga
     1441 aaatgaaggt aaaattgagt ttagggtggt gaataatgat aatactaaag aaaacatgat
     1501 ggtcctaact ggattaaaaa acatttttca aaagcaatta ccaaaaatgc ccaaagaata
     1561 cattgccagg ttagtctatg atcgaagtca tctttccatg gctgtcatta ggaagccatt
     1621 gactgtcgta ggtggcataa catatcgacc tttcgataag agagaattcg cagaaattgt
     1681 tttctgtgcc atcagttcga cggaacaggt acgcggttat ggtgcgcatc taatgaatca
     1741 cttaaaagac tatgttagaa atacctcgaa cataaaatat tttttgacat atgcagataa
     1801 ttacgctatt ggatacttta aaaagcaagg ctttactaaa gaaatcacgt tggataaaag
     1861 tatatggatg ggatatatta aagattatga aggtggtacg ctgatgcaat gtaacatggc
     1921 aattcccggt ggcggccgca tcttttaccc atacgatgtt cctgactatg cgggctatcc
     1981 ctatgacgtc ccggactatg caggatccta tccatatgac gttccagatt acgctgctca
     2041 gtgcggccgc tctagctaga actagtggat cccccgatac cgtcgacctg cagagatcta
     2101 tgaatcgtag atactgaaaa accccgcaag ttcacttcaa ctgtgcatcg tgcaccatct
     2161 caatttcttt catttataca tcgttttgcc ttcttttatg taactatact cctctaagtt
     2221 tcaatcttgg ccatgtaacc tctgatctat agaatttttt aaatgactag aattaatgcc
     2281 catctttttt ttggacctaa attcttcatg aaaatatatt acgagggctt attcagaagc
     2341 tttggacttc ttcgccagag gtttggtcaa gtctccaatc aaggttgtcg gcttgtctac
     2401 cttgccagaa atttacgaaa agatggaaaa gggtcaaatc gttggtagat acgttgttga
     2461 cacttctaaa taagcgaatt tcttatgatt tatgattttt attattaaat aagttataaa
     2521 aaaaataagt gtatacaaat tttaaagtga ctcttaggtt ttaaaacgaa aattcttatt
     2581 cttgagtaac tctttcctgt aggtcaggtt gctttctcag gtatagcatg aggtcgctct
     2641 tattgaccac acctctaccg gcatgccgag caaatgcctg caaatcgctc cccatttcac
     2701 ccaattgtag atatgctaac tccagcaatg agttgatgaa tctcggtgtg tattttatgt
     2761 cctcagagga caacacctgt tgtaatcgtt cttccacacg gatcctggcg taatagcgaa
     2821 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg
     2881 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tcgctgggcc
     2941 attctcatga agaatatctt gaatttattg tcatattact agttggtgtg gaagtccata
     3001 tatcggtgat caatatagtg gttgacatgc tggctagtca acattgagcc ttttgatcat
     3061 gcaaatatat tacggtattt tacaatcaaa tatcaaactt aactattgac tttataactt
     3121 atttaggtgg taacattctt ataaaaaaga aaaaaattac tgcaaaacag tactagcttt
     3181 taacttgtat cctaggttat ctatgctgtc tcaccataga gaatattacc tatttcagaa
     3241 tgtatgtcca tgattcgccg ggtaaataca tataatacac aaatctggct taataaagtc
     3301 tataatatat ctcataaaga agtgctaaat tggctagtgc tatatatttt taagaaaatt
     3361 tcttttgact aagtccatat cgactttgta aaagttcact ttagcataca tatattacac
     3421 gagccagaaa ttgtaacttt tgcctaaaat cacaaattgc aaaatttaat tgcttgcaaa
     3481 aggtcacatg cttataatca acttttttaa aaatttaaaa tactttttta ttttttattt
     3541 ttaaacataa atgaaataat ttatttattg tttatgatta ccgaaacata aaacctgctc
     3601 aagaaaaaga aactgttttg tccttggaaa aaaagcacta cctaggagcg gccaaaatgc
     3661 cgaggctttc atagcttaaa ctctttacag aaaataggca ttatagatca gttcgagttt
     3721 tcttattctt ccttccggtt ttatcgtcac agttttacag taaataagta tcacctctta
     3781 gagttcgatg ataagctgtc aaacatgaga attaattcca catgttaaaa tagtgaagga
     3841 gcatgttcgg cacacagtgg accgaacgtg gggtaagtgc actagggtcc ggttaaacgg
     3901 atctcgcatt gatgaggcaa cgctaattat caacatatag attgttatct atctgcatga
     3961 acacgaaatc tttacttgac gacttgaggc tgatggtgtt tatgcaaaga aaccactgtg
     4021 tttaatatgt gtcactgttt gatattactg tcagcgtaga agataatagt aaaagcggtt
     4081 aataagtgta tttgagataa gtgtgataaa gtttttacag cgaaaagacg ataaatacaa
     4141 gaaaatgatt acgaggatac ggagagaggt atgtacatgt gtatttatat actaagctgc
     4201 cggcggttgt ttgcaagacc gagaaaaggc tagcaagaat cgggtcattg tagcgtatgc
     4261 gcctgtgaac attctcttca acaagtttga ttccattgcg gtgaaatggt aaaagtcaac
     4321 cccctgcgat gtatattttc ctgtacaatc aatcaaaaag ccaaatgatt tagcattatc
     4381 tttacatctt gttattttac agattttatg tttagatctt ttatgcttgc ttttcaaaag
     4441 gcttgcaggc aagtgcacaa acaatactta aataaatact actcagtaat aacctatttc
     4501 ttagcatttt tgacgaaatt tgctattttg ttagagtctt ttacaccatt tgtctccaca
     4561 cctccgctta catcaacacc aataacgcca tttaatctaa gcgcatcacc aacattttct
     4621 ggcgtcagtc caccagctaa cataaaatgt aagctctcgg ggctctcttg ccttccaacc
     4681 cagtcagaaa tcgagttcca atccaaaagt tcacctgtcc cacctgcttc tgaatcaaac
     4741 aagggaataa acgaatgagg tttctgtgaa gctgcactga gtagtatgtt gcagtctttt
     4801 ggaaatacga gtcttttaat aactggcaaa ccgaggaact cttggtattc ttgccacgac
     4861 tcatctccat gcagttggac gatatcaatg ccgtaatcat tgaccagagc caaaacatcc
     4921 tccttaggtt gattacgaaa cacgccaacc aagtatttcg gagtgcctga actattttta
     4981 tatgctttta caagacttga aattttcctt gcaataaccg ggtcaattgt tctctttcta
     5041 ttgggcacac atataatacc cagcaagtca gcatcggaat ctagtgcaca ttctgcggcc
     5101 tctgtgctct gcaagccgca aactttcacc aatggaccag aactacctgt gaaattaata
     5161 acagacatac tccaagctgc ctttgtgtgc ttaatcacgt atactcacgt gctcaatagt
     5221 caccaatgcc ctccctcttg gccctctcct tttctttttt cgaccgaatt aattcttgaa
     5281 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     5341 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     5401 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     5461 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     5521 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     5581 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     5641 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     5701 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     5761 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     5821 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     5881 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     5941 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     6001 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     6061 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     6121 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     6181 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     6241 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     6301 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     6361 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     6421 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     6481 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     6541 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     6601 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     6661 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     6721 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     6781 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     6841 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     6901 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     6961 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     7021 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     7081 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     7141 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     7201 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     7261 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     7321 cgattcatta atgcaggatc cgggatcgaa gaaatgatgg taaatgaaat aggaaatcaa
     7381 ggagcatgaa ggcaaaagac aaatataagg gtcgaacgaa aaataaagtg aaaagtgttg
     7441 atatgatgta tttggctttg cggcgccgaa aaaacgagtt tacgcaattg cacaatcatg
     7501 ctgactctgt ggcggacccg cgctcttgcc ggcccggcga taacgctggg cgtgaggctg
     7561 tgcccggcgg agttttttgc gcctgcattt tccaaggttt accctgcgct aaggggcgag
     7621 attggagaag caataagaat gccggttggg gttgcgatga tgacgaccac gacaactggt
     7681 gtcattattt aagttgccga aagaacctga gtgcatttgc aacatgagta tactagaaga
     7741 atgagccaag acttgcgaga cgcgagtttg ccggtggtgc gaacaataga gcgaccatga
     7801 ccttgaaggt gagacgcgca taaccgctag agtactttga agaggaaaca gcaatagggt
     7861 tgctaccagt ataaatagac aggtacatac aacactggaa atggttgtct gtttgagtac
     7921 gctttcaatt catttgggtg tgcactttat tatgttacaa tatggaaggg aactttacac
     7981 ttctcctatg cacatatatt aattaaagtc caatgctagt agagaagggg ggtaacaccc
     8041 ctccgcgctc ttttccgatt tttttctaaa ccgtggaata tttcggatat ccttttgttg
     8101 tttccgggtg tacaatatgg acttcctctt ttctggcaac caaacccata catcgggatt
     8161 cctataatac cttcgttggt ctccctaaca tgtaggtggc ggaggggaga tatacaatag
     8221 aacagatacc agacaagaca taatgggcta aacaagacta caccaattac actgcctcat
     8281 tgatg
//
