LOCUS       V006998                 6133 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V006998
VERSION     V006998
KEYWORDS    pDG02583
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6133)
  AUTHORS   Pleiner T,  Bates M,  Gorlich D
  TITLE     A toolbox of anti–mouse and anti–rabbit IgG secondary nanobodies
  JOURNAL   J Cell Biol Dec 2017, jcb.201709115
REFERENCE   2  (bases 1 to 6133)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6133)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Biol
            Dec 2017, jcb.201709115"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6133
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1004..1081)
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     misc_feature    1273..1413
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1599..2187)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        2320..2364
                     /label="T5 promoter"
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     CDS             2503..3600
                     /label="MBP"
                     /note="maltose binding protein from E. coli"
     CDS             3613..3897
                     /gene="SUMO1"
                     /label="Small ubiquitin-related modifier 1"
                     /note="Small ubiquitin-related modifier 1 from Oryza sativa
                     subsp. japonica. Accession#: P55857"
     terminator      4688..4782
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             4841..5653
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     terminator      5875..5961
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    complement(6020..6041)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(join(6057..6133,1..1003))
                     /label="lacI"
                     /note="lac repressor"
ORIGIN
        1 gcgtattggg ggccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc
       61 ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca
      121 ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat
      181 cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc
      241 gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct
      301 cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt
      361 ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac
      421 gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga
      481 ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg
      541 gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag
      601 caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga
      661 gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca
      721 ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg
      781 cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt
      841 gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc
      901 gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga
      961 caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt
     1021 gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcaccat tcgatggtgt
     1081 cggaatttcg ggcagcgttg ggtcctggcc acgggtgcgc atgatctaga gctgcctcgc
     1141 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggcat ccgcttacag
     1201 acaagctgtg accgtctccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     1261 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt
     1321 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     1381 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac
     1441 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     1501 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     1561 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     1621 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1681 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1741 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1801 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1861 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1921 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     1981 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     2041 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     2101 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     2161 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     2221 gctcagtgga acgaaaactc acgttaaggg attttggtca tgacattaac ctataaaaat
     2281 aggcgtatca cgaggccctt tcgtcttcac ctcgagaaat cataaaaaat ttatttgctt
     2341 tgtgagcgga taacaattat aatagattca attgtgagcg gataacaatt tcacacagaa
     2401 ttcattaaag aggagaaatt aaccatgagc aagcatcacc atcattcagg ccatcaccat
     2461 accggacacc accatcattc aggcagtcat caccataccg ggaaaactga agaaggtaaa
     2521 ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa
     2581 ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa
     2641 ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc
     2701 tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag
     2761 gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac
     2821 ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca
     2881 aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg
     2941 ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt
     3001 tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct
     3061 ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca
     3121 gacactgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc
     3181 aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta
     3241 ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt
     3301 aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact
     3361 gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct
     3421 tacgaggaag agttggcgaa agatccacgt attgccgcca ccatggaaaa cgcccagaaa
     3481 ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg
     3541 gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact
     3601 aattcgagct cagctgcggg tggcgaagaa gataagaaac cggcaggtgg cgaaggtggc
     3661 ggtgcccata tcaacctgaa agtgaaaggt caagacggca acgaagtctt tttccgcatc
     3721 aaacgttcta cccagctgaa aaagctgatg aacgcatact gtgaccgtca gtctgtagac
     3781 atgaccgcaa ttgctttcct gtttgatggt cgtcgcctgc gtgcggaaca gaccccggat
     3841 gaactggaga tggaagatgg cgacgaaatc gacgcaatgc ttcatcagac tggtggcggt
     3901 ggaatggatg aacgtgtcct gtcttatggt gatgtggtgc tccttcgttc cgatctggct
     3961 attctgcgtg gcccgcactt cctgaacgac cgtattatcg ccttctatct ggcacacctg
     4021 tccgcttcct tccacggtga tggcgatctg ctgctcctgc cgccgagcat tccgtacctg
     4081 ctgtccaacc tgccggaccc ggaatctgtc gcagaaccac tgtgtctggc atcccgccgt
     4141 ctggtcctgc tgccggttaa cgataacccg gacgcctcgg tggcaaatgg cggttctcac
     4201 tggactctgc tcgtcctgga cgctgcgact actgacccgc aggctccgcg tttcgtgcat
     4261 catgactccc tgcgcggttc cgcgaacgcg gctgctgcgc gtcgcctggc ccgtgctctg
     4321 accgctggtg gtgcgccgct gcgcttcgtt gaggctccta ccccgaccca gcgtaacggc
     4381 cacgactgcg gcgtctatgt cctggccgta gctcgtgcta tttgtggttg gtggcgttct
     4441 tctcgtcgcc gcgaaaacca gcagggcggt ggcggcgatt ggtttgcaac catgatggaa
     4501 gaagtggatg ctgaatccgt aggcgctatg cgcgctgaac tgctccagct cattcaccgt
     4561 ctgattcagg acaaggaaca agaagaagag aaaaagtcca aagctggcgt ggaggatacc
     4621 tgcggccaat aactcgtagg ctcgacacga agcatagcgg ccgcataagc ttaattagct
     4681 gagcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
     4741 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaagcta gccatgaaaa
     4801 taaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga
     4861 aacgtcttgc tctaggccgc gattaaattc caacatggat gctgatttat atgggtataa
     4921 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc
     4981 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga
     5041 tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt
     5101 tatccgtact cctgatgatg cttggttact cacgactgcg atccccggca aaacagcatt
     5161 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt
     5221 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt
     5281 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga
     5341 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcaca aacttttgcc
     5401 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga
     5461 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca
     5521 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct
     5581 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct
     5641 cgatgagttt ttctaagaat taattcatgg gcaaatatta tacgcaaggc gacaaggtgc
     5701 tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa
     5761 tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa tttttttaag
     5821 gcagttattg gtgcccttaa acgcctgggg taatgactct ctagcttgag gcatcaaata
     5881 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     5941 gctctcctga gtaggacaaa tccgccctct agattacgtg cagtcgatga taagctgtca
     6001 aacatgagaa ttgtgcctaa tgagtgagct aacttacatt aattgcgttg cgctcactgc
     6061 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     6121 ggagaggcgg ttt
//
