LOCUS       V016792                 6859 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V016792
VERSION     V016792
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6859)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6859
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        105..484
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        485..688
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        733..751
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             809..1861
                     /gene="MCU"
                     /label="Calcium uniporter protein, mitochondrial"
                     /note="Calcium uniporter protein, mitochondrial from Homo
                     sapiens. Accession#: Q8NE86"
     protein_bind    complement(1866..1886)
                     /label="attB2"
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     CDS             1901..2617
                     /label="EmGFP"
                     /note="Emerald GFP"
     polyA_signal    2711..2759
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      2961..3389
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3403..3732
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        3780..3827
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             3846..4241
                     /label="BSD"
                     /note="blasticidin S deaminase"
     polyA_signal    4402..4535
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4572..4588)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4596..4612)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4620..4650)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4665..4686)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4974..5562)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5736..6593)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6594..6698)
                     /label="AmpR promoter"
ORIGIN
        1 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
       61 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac
      121 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
      181 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      241 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      301 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      361 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      421 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      481 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      541 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      601 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      661 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      721 gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt taagctgagc
      781 atcaacaagt ttgtacaaaa aagttggcat ggcggccgcc gcaggtagat cgctcctgct
      841 gctcctctcc tctcggggcg gcggcggcgg gggcgccggc ggctgcgggg cgctgactgc
      901 cggctgcttc cctgggctgg gcgtcagccg ccaccggcag cagcagcacc accggacggt
      961 acaccagagg atcgcttcct ggcagaattt gggagctgtt tattgcagca ctgttgtgcc
     1021 ctctgatgat gttacagtgg tttatcaaaa tgggttacct gtgatatctg tgaggctacc
     1081 atcccggcgt gaacgctgtc agttcacact caagcctatc tctgactctg ttggtgtatt
     1141 tttacgacaa ctgcaagaag aggatcgggg aattgacaga gttgctatct attcaccaga
     1201 tggtgttcgc gttgctgctt caacaggaat agacctcctc ctccttgatg actttaagct
     1261 ggtcattaat gacttaacat accacgtacg accaccaaaa agagacctct taagtcatga
     1321 aaatgcagca acgctgaatg atgtaaagac attggtccag caactataca ccacactgtg
     1381 cattgagcag caccagttaa acaaggaaag ggagcttatt gaaagactag aggatctcaa
     1441 agagcagctg gctcccctgg aaaaggtacg aattgagatt agcagaaaag ctgagaagag
     1501 gaccactttg gtgctatggg gtggccttgc ctacatggcc acacagtttg gcattttggc
     1561 ccggcttacc tggtgggaat attcctggga catcatggag ccagtaacat acttcatcac
     1621 ttatggaagt gccatggcaa tgtatgcata ttttgtaatg acacgccagg aatatgttta
     1681 tccagaagcc agagacagac aatacttact atttttccat aaaggagcca aaaagtcacg
     1741 ttttgaccta gagaaataca atcaactcaa ggatgcaatt gctcaggcag aaatggacct
     1801 taagagactg agagacccat tacaagtaca tctgcctctc cgacaaattg gtgaaaaaga
     1861 ttgcccaact ttcttgtaca aagtggttga tgctgttaac atggtgagca agggcgagga
     1921 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     1981 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     2041 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcaccta
     2101 cggcgtgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     2161 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     2221 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     2281 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     2341 cagccacaag gtctatatca ccgccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     2401 gacccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     2461 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     2521 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     2581 cgccgggatc actctcggca tggacgagct gtacaagtaa tgataagttt aaacgggccc
     2641 gcggttcgct gatgggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc
     2701 cgcgctatga cggcaataaa aagacagaat aaaacgcacg ggtgttgggt cgtttgttca
     2761 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg
     2821 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg
     2881 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcagatc tgcgcagctg
     2941 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     3001 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     3061 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     3121 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     3181 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     3241 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     3301 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     3361 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
     3421 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     3481 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     3541 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     3601 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     3661 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     3721 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg
     3781 ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact
     3841 aaaccatggc caagcctttg tctcaagaag aatccaccct cattgaaaga gcaacggcta
     3901 caatcaacag catccccatc tctgaagact acagcgtcgc cagcgcagct ctctctagcg
     3961 acggccgcat cttcactggt gtcaatgtat atcattttac tgggggacct tgtgcagaac
     4021 tcgtggtgct gggcactgct gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga
     4081 tcggaaatga gaacaggggc atcttgagcc cctgcggacg gtgccgacag gtgcttctcg
     4141 atctgcatcc tgggatcaaa gccatagtga aggacagtga tggacagccg acggcagttg
     4201 ggattcgtga attgctgccc tctggttatg tgtgggaggg ctaagcactt cgtggccgag
     4261 gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg
     4321 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     4381 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     4441 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     4501 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     4561 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     4621 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     4681 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     4741 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     4801 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     4861 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     4921 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     4981 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     5041 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     5101 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     5161 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     5221 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     5281 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     5341 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     5401 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     5461 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     5521 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     5581 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     5641 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     5701 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     5761 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     5821 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     5881 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     5941 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     6001 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     6061 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     6121 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     6181 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     6241 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     6301 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     6361 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     6421 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     6481 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     6541 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     6601 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     6661 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     6721 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct
     6781 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
     6841 agtagtgcgc gagcaaaat
//
