LOCUS       40924_14450       12084 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pDEST374-U1Ux, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12084)
  AUTHORS   MacPherson MI, Saka Y.
  TITLE     Short Synthetic Terminators for Assembly of Transcription Units in 
            vitro and Stable Chromosomal Integration in Yeast S. cerevisiae
  JOURNAL   ACS Synth Biol (2016) In press
  PUBMED    27529501
REFERENCE   2  (bases 1 to 12084)
  AUTHORS   Saka Y.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 12084)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12084)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol (2016) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-MAY-2016) School of Medicine, Medical Sciences "
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12084
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(71..466)
                     /label=HIS3 YIp-In
                     /note="HIS3 YIp-In"
     misc_feature    474..513
                     /label=U1 sequence
                     /note="U1 sequence"
     misc_feature    530..569
                     /label=Ux sequence
                     /note="Ux sequence"
     promoter        complement(894..1079)
                     /label=HIS3 promoter
     CDS             1138..1155
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      1222..1269
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(1646..2521)
                     /codon_start=1
                     /label=araC
                     /note="L-arabinose regulatory protein"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        2548..2832
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     CDS             2892..4037
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWLQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMDFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(4211..4867)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(4868..4970)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     promoter        5286..5501
                     /label=URA3 promoter
     CDS             5502..6302
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(6957..7669)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      7748..7967
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             8058..8810
                     /codon_start=1
                     /label=repE
                     /note="replication initiation protein for the bacterial F 
                     plasmid"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    8816..9066
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             9392..10564
                     /codon_start=1
                     /label=sopA
                     /note="partitioning protein for the bacterial F plasmid"
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             10567..11535
                     /codon_start=1
                     /label=sopB
                     /note="partitioning protein for the bacterial F plasmid"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    11611..12084
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
ORIGIN
        1 gatcagacta tcagcgtgag actacgattc catcaatgcc tgtcaagggc aagtattgac
       61 atgtgcggcc gcagatcctc ctcttaagtg aggaggaaca taaccattct cgttgttgtc
      121 gttgatgctt aaattttgca cttgttcgct cagttcagcc ataatatgaa atgcttttct
      181 tgttgttctt acggaatacc acttgccacc tatcaccaca actaactttt tcccgttcct
      241 ccatctcttt tatatttttt ttctcgactc ctcggcgcgc cgaggagtcg agttcaagag
      301 aaaaaaaaag aaaaagcaaa aagaaaaaag gaaagcgcgc ctcgttcaga atgacacgta
      361 tagaatgatg cattaccttg tcatcttcag tatcatactg ttcgtataca tactactgac
      421 attcataggt atacatatat acacatgtat atatatcgta tgcctgcggc cgctaagact
      481 ttatcaacgc tcacaatcgt agaggagtaa cttcctaggt caacgtacga taagtgattt
      541 caactgagtg ctttacgact gctacaacag ctaggccgca ctcgagcagg gaccgagagc
      601 aatcccgcag tcttcagtgg tgtgatggtc gtctatgtgt aagtcaccaa tgcactcaac
      661 gattagcgac cagccggaat gcttggccag agcatgtatc atatggtcca gaaaccctat
      721 acctgtgtgg acgttaatca cttgcgattg tgtggcctgt tctgctactg cttctgcctc
      781 ttttctggga agatcgagtg ctctatcgct aggggaccac cctttaaaga gatcgcaatc
      841 tgaatcttgg tttcatttgt aatacgcttt actagggctt tctgctctgt catctttgcc
      901 ttcgtttatc ttgcctgctc attttttagt atattcttcg aagaaatcac attactttat
      961 ataatgtata attcattatg tgataatgcc aatcgctaag aaaaaaaaag agtcatccgc
     1021 taggtggaaa aaaaaatgaa aatcattacc gaggcataaa aaaatataga gtgtactaga
     1081 ggaggccaag agtaatagaa aaagaaaatt gcgggaaagg actgctcgac tctcgagcac
     1141 caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct
     1201 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg
     1261 ggttttttgc tgaaaggagg aactatatcc ggattggcga atgggacgcg ccctgtagcg
     1321 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     1381 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     1441 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     1501 tcgaccccaa aaaacttgat tagggtgatg gttcacggcc gccgcaatgc ttgcataatg
     1561 tggcctgtca aatggacgaa gcagggattc tgcaaaccct atgctactcc gtcaagccgt
     1621 caattgtctg attcgttacc aattatgaca acttgacggc tacatcattc actttttctt
     1681 cacaaccggc acgaaactcg ctcgggctgg ccccggtgca ttttttaaat actcgcgaga
     1741 aatagagttg atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc atccgggtag
     1801 tgctcaaaag cagcttcgcc tggctgatac gttggtcctc gcgccagctt aagacgctaa
     1861 tccctaactg ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg
     1921 cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct
     1981 cgcgtacccg attatccatc ggcggatgga gcgactcgtt aatcgcttcc atgcgccgca
     2041 gtaacaattg ctcaagcaga tttatcgcca gcagctccga atagcgccct tccccttgcc
     2101 cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc
     2161 gaaagaaacc cgtattggca aatattgacg gccagttaag ccattcatgc cagtaggcgc
     2221 gcggacgaaa gtaaacccac tggtgatacc attcgcgagc ctccggatga cgaccgtagt
     2281 gatgaatctc tcctggcggg aacagcaaaa tatcacccgg tcggcagaca aattctcgtc
     2341 cctgattttt caccaccccc tgaccgcgaa tggtgagatt gagaatataa cctttcattc
     2401 ccagcggtcg gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc gttaaacccg
     2461 ccaccagatg ggcgttaaac gagtatcccg gcagcagggg atcattttgc gcttcagcca
     2521 tacttttcat actcccacca ttcagagaag aaaccaattg tccatattgc atcagacatt
     2581 gccgtcactg cgtcttttac tggctcttct cgctaaccca accggtaacc ccgcttatta
     2641 aaagcattct gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct
     2701 ataatcacgg cagaaaagtc cacattgatt atttgcacgg cgtcacactt tgctatgcca
     2761 tagcattttt atccataaga ttagcggatc ctacctgacg ctttttatcg caactctcta
     2821 ctgtttctcc atacccgttt ttttggatgg agtgaaagga ggaattcggg gcaatcccgc
     2881 aaggagggtg aatgaatcgg acgtttgacc ggaaggcata caggcaagaa ctgatcgacg
     2941 cggggttttc cgccgaggat gccgaaacca tcgcaagccg caccgtcatg cgtgcgcccc
     3001 gcgaaacctt ccagtccgtc ggctcgatgg tccagcaagc tacggccaag atcgagcgcg
     3061 acagcgtgca actggctccc cctgccctgc ccgcgccatc ggccgccgtg gagcgttcgc
     3121 gtcgtctcga acaggaggcg gcaggtttgg cgaagtcgat gaccatcgac acgcgaggaa
     3181 ctatgacgac caagaagcga aaaaccgccg gcgaggacct ggcaaaacag gtcagcgagg
     3241 ccaagcaggc cgcgttgctg aaacacacga agcagcagat caaggaaatg cagctttcct
     3301 tgttcgatat tgcgccgtgg ccggacacga tgcgagcgat gccaaacgac acggcccgct
     3361 ctgccctgtt caccacgcgc aacaagaaaa tcccgcgcga ggcgctgcaa aacaaggtca
     3421 ttttccacgt caacaaggac gtgaagatca cctacaccgg cgtcgagctg cgggccgacg
     3481 atgacgaact ggtgtggctg caggtgttgg agtacgcgaa gcgcacccct atcggcgagc
     3541 cgatcacctt cacgttctac gagctttgcc aggacctggg ctggtcgatc aatggccggt
     3601 attacacgaa ggccgaggaa tgcctgtcgc gcctacaggc gacggcgatg gacttcacgt
     3661 ccgaccgcgt tgggcacctg gaatcggtgt cgctgctgca ccgcttccgc gtcctggacc
     3721 gtggcaagaa aacgtcccgt tgccaggtcc tgatcgacga ggaaatcgtc gtgctgtttg
     3781 ctggcgacca ctacacgaaa ttcatatggg agaagtaccg caagctgtcg ccgacggccc
     3841 gacggatgtt cgactatttc agctcgcacc gggagccgta cccgctcaag ctggaaacct
     3901 tccgcctcat gtgcggatcg gattccaccc gcgtgaagaa gtggcgcgag caggtcggcg
     3961 aagcctgcga agagttgcga ggcagcggcc tggtggaaca cgcctgggtc aatgatgacc
     4021 tggtgcattg caaacgctag ggccttgtgg ggtcagttcc ggctgggggt tcagcagcca
     4081 ccgggccgtc gaccaattct catgtttgac agcttatcat cgaatttctg ccattcatcc
     4141 gcttattatc acttattcag gcgtagcaac caggcgttta agggcaccaa taactgcctt
     4201 aaaaaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc
     4261 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
     4321 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
     4381 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa
     4441 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     4501 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     4561 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca
     4621 ccagctcacc gtctttcatt gccatacgga attccggatg agcattcatc aggcgggcaa
     4681 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     4741 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     4801 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt
     4861 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta
     4921 gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgccgatca acgtctcatt
     4981 ttcgccaaaa gttggcccag ggcttcccgg tatcaacagg gacaccagga tttatttatt
     5041 ctgcgaagtg atcttccgtc acaggtattt attcgcgata agctcatgga gcggcgtaac
     5101 cgtcgcacag gaaggacaga gaaagcgcgg atctgggaag tgacggacag aacggtcagg
     5161 acctggattg gggaggcggt tgccgccgct gctgctgacg gtgtgacgtt ctctgttccg
     5221 gtcacaccac atacgttccg ccattcctat gcgatgcaca tgctgtatgc cggtaaccac
     5281 agcttttcaa ttcatcattt tttttttatt cttttttttg atttcggttt ccttgaaatt
     5341 tttttgattc ggtaatctcc gaacagaagg aagaacgaag gaaggagcac agacttagat
     5401 tggtatatat acgcatatgt agtgttgaag aaacatgaaa ttgcccagta ttcttaaccc
     5461 aactgcacag aacaaaaacc tgcaggaaac gaagataaat catgtcgaaa gctacatata
     5521 aggaacgtgc tgctactcat cctagtcctg ttgctgccaa gctatttaat atcatgcacg
     5581 aaaagcaaac aaacttgtgt gcttcattgg atgttcgtac caccaaggaa ttactggagt
     5641 tagttgaagc attaggtccc aaaatttgtt tactaaaaac acatgtggat atcttgactg
     5701 atttttccat ggagggcaca gttaagccgc taaaggcatt atccgccaag tacaattttt
     5761 tactcttcga agacagaaaa tttgctgaca ttggtaatac agtcaaattg cagtactctg
     5821 cgggtgtata cagaatagca gaatgggcag acattacgaa tgcacacggt gtggtgggcc
     5881 caggtattgt tagcggtttg aagcaggcgg cagaagaagt aacaaaggaa cctagaggcc
     5941 ttttgatgtt agcagaattg tcatgcaagg gctccctatc tactggagaa tatactaagg
     6001 gtactgttga cattgcgaag agcgacaaag attttgttat cggctttatt gctcaaagag
     6061 acatgggtgg aagagatgaa ggttacgatt ggttgattat gacacccggt gtgggtttag
     6121 atgacaaggg agacgcattg ggtcaacagt atagaaccgt ggatgatgtg gtctctacag
     6181 gatctgacat tattattgtt ggaagaggac tatttgcaaa gggaagggat gctaaggtag
     6241 agggtgaacg ttacagaaaa gcaggctggg aagcatattt gagaagatgc ggccagcaaa
     6301 actaaaaaac tgtattataa gtaaatgcat gtatactaaa ctcacaaatt agagcttcaa
     6361 tttaattata tcagttatta ccctatgcgg tgtgaaatta ccgctgaaag ttctgcaaag
     6421 cctgatggga cataagtcca tcagttcaac ggaagtctac acgaaggttt ttgcgctgga
     6481 tgtggctgcc cggcaccggg tgcagtttgc gatgccggag tctgatgcgg ttgcgatgct
     6541 gaaacaatta tcctgagaat aaatgccttg gcctttatat ggaaatgtgg aactgagtgg
     6601 atatgctgtt tttgtctgtt aaacagagaa gctggctgtt atccactgag aagcgaacga
     6661 aacagtcggg aaaatctccc attatcgtag agatccgcat tattaatctc aggagcctgt
     6721 gtagcgttta taggaagtag tgttctgtca tgatgcctgc aagcggtaac gaaaacgatt
     6781 tgaatatgcc ttcaggaaca atagaaatct tcgtgcggtg ttacgttgaa gtggagcgga
     6841 ttatgtcagc aatggacaga acaacctaat gaacacagaa ccatgatgtg gtctgtcctt
     6901 ttacagccag tagtgctcgc cgcagtcgag cgacagggcg aagccctcga gatcccctgc
     6961 catttttggg gtgaggccgt tcgcggccga ggggcgcagc ccctgggggg atgggaggcc
     7021 cgcgttagcg gccgggaggg ttcgagaagg gggggcaccc cccttcggcg tgcgcggtca
     7081 cgcgcacagg gcgcagccct ggttaaaaac aaggtttata aatattggtt taaaagcagg
     7141 ttaaaagaca ggttagcggt ggccgaaaaa cgggcggaaa cccttgcaaa tgctggattt
     7201 tctgcctgtg gacagcccct caaatgtcaa taggtgcgcc cctcatctgt cagcactctg
     7261 cccctcaagt gtcaaggatc gcgcccctca tctgtcagta gtcgcgcccc tcaagtgtca
     7321 ataccgcagg gcacttatcc ccaggcttgt ccacatcatc tgtgggaaac tcgcgtaaaa
     7381 tcaggcgttt tcgccgattt gcgaggctgg ccagctccac gtcgccggcc gaaatcgagc
     7441 ctgcccctca tctgtcaacg ccgcgccggg tgagtcggcc cctcaagtgt caacgtccgc
     7501 ccctcatctg tcagtgaggg ccaagttttc cgcgaggtat ccacaacgcc ggcggccggc
     7561 cgcggtgtct cgcacacggc ttcgacggcg tttctggcgc gtttgcaggg ccatagacgg
     7621 ccgccagccc agcggcgagg gcaaccagcc cggtgagcgt cggaaagggg aatttcgagt
     7681 gagcgaggaa gcaccaggga acagcactta tatattctgc ttacacacga tgcctgaaaa
     7741 aacttccctt ggggttatcc acttatccac ggggatattt ttataattat tttttttata
     7801 gtttttagat cttctttttt agagcgcctt gtaggccttt atccatgctg gttctagaga
     7861 aggtgttgtg acaaattgcc ctttcagtgt gacaaatcac cctcaaatga cagtcctgtc
     7921 tgtgacaaat tgcccttaac cctgtgacaa attgccctca gaagaagctg ttttttcaca
     7981 aagttatccc tgcttattga ctctttttta tttagtgtga caatctaaaa acttgtcaca
     8041 cttcacatgg atctgtcatg gcggaaacag cggttatcaa tcacaagaaa cgtaaaaata
     8101 gcccgcgaat cgtccagtca aacgacctca ctgaggcggc atatagtctc tcccgggatc
     8161 aaaaacgtat gctgtatctg ttcgttgacc agatcagaaa atctgatggc accctacagg
     8221 aacatgacgg tatctgcgag atccatgttg ctaaatatgc tgaaatattc ggattgacct
     8281 ctgcggaagc cagtaaggat atacggcagg cattgaagag tttcgcgggg aaggaagtgg
     8341 ttttttatcg ccctgaagag gatgccggcg atgaaaaagg ctatgaatct tttccttggt
     8401 ttatcaaacg tgcgcacagt ccatccagag ggctttacag tgtacatatc aacccatatc
     8461 tcattccctt ctttatcggg ttacagaacc ggtttacgca gtttcggctt agtgaaacaa
     8521 aagaaatcac caatccgtat gccatgcgtt tatacgaatc cctgtgtcag tatcgtaagc
     8581 cggatggctc aggcatcgtc tctctgaaaa tcgactggat catagagcgt taccagctgc
     8641 ctcaaagtta ccagcgtatg cctgacttcc gccgccgctt cctgcaggtc tgtgttaatg
     8701 agatcaacag cagaactcca atgcgcctct catacattga gaaaaagaaa ggccgccaga
     8761 cgactcatat cgtattttcc ttccgcgata tcacttccat gacgacagga tagtctgagg
     8821 gttatctgtc acagatttga gggtggttcg tcacatttgt tctgacctac tgagggtaat
     8881 ttgtcacagt tttgctgttt ccttcagcct gcatggattt tctcatactt tttgaactgt
     8941 aatttttaag gaagccaaat ttgagggcag tttgtcacag ttgatttcct tctctttccc
     9001 ttcgtcatgt gacctgatat cgggggttag ttcgtcatca ttgatgaggg ttgattatca
     9061 cagtttatta ctctgaattg gctatccgcg tgtgtacctc tacctggagt ttttcccacg
     9121 gtggatattt cttcttgcgc tgagcgtaag agctatctga cagaacagtt cttctttgct
     9181 tcctcgccag ttcgctcgct atgctcggtt acacggctgc ggcgagcgct agtgataata
     9241 agtgactgag gtatgtgctc ttcttatctc cttttgtagt gttgctctta ttttaaacaa
     9301 ctttgcggtt ttttgatgac tttgcgattt tgttgttgct ttgcagtaaa ttgcaagatt
     9361 taataaaaaa acgcaaagca atgattaaag gatgttcaga atgaaactca tggaaacact
     9421 taaccagtgc ataaacgctg gtcatgaaat gacgaaggct atcgccattg cacagtttaa
     9481 tgatgacagc ccggaagcga ggaaaataac ccggcgctgg agaataggtg aagcagcgga
     9541 tttagttggg gtttcttctc aggctatcag agatgccgag aaagcagggc gactaccgca
     9601 cccggatatg gaaattcgag gacgggttga gcaacgtgtt ggttatacaa ttgaacaaat
     9661 taatcatatg cgtgatgtgt ttggtacgcg attgcgacgt gctgaagacg tatttccacc
     9721 ggtgatcggg gttgctgccc ataaaggtgg cgtttacaaa acctcagttt ctgttcatct
     9781 tgctcaggat ctggctctga aggggctacg tgttttgctc gtggaaggta acgaccccca
     9841 gggaacagcc tcaatgtatc acggatgggt accagatctt catattcatg cagaagacac
     9901 tctcctgcct ttctatcttg gggaaaagga cgatgtcact tatgcaataa agcccacttg
     9961 ctggccgggg cttgacatta ttccttcctg tctggctctg caccgtattg aaactgagtt
    10021 aatgggcaaa tttgatgaag gtaaactgcc caccgatcca cacctgatgc tccgactggc
    10081 cattgaaact gttgctcatg actatgatgt catagttatt gacagcgcgc ctaacctggg
    10141 tatcggcacg attaatgtcg tatgtgctgc tgatgtgctg attgttccca cgcctgctga
    10201 gttgtttgac tacacctccg cactgcagtt tttcgatatg cttcgtgatc tgctcaagaa
    10261 cgttgatctt aaagggttcg agcctgatgt acgtattttg cttaccaaat acagcaatag
    10321 taatggctct cagtccccgt ggatggagga gcaaattcgg gatgcctggg gaagcatggt
    10381 tctaaaaaat gttgtacgtg aaacggatga agttggtaaa ggtcagatcc ggatgagaac
    10441 tgtttttgaa caggccattg atcaacgctc ttcaactggt gcctggagaa atgctctttc
    10501 tatttgggaa cctgtctgca atgaaatttt cgatcgtctg attaaaccac gctgggagat
    10561 tagataatga agcgtgcgcc tgttattcca aaacatacgc tcaatactca accggttgaa
    10621 gatacttcgt tatcgacacc agctgccccg atggtggatt cgttaattgc gcgcgtagga
    10681 gtaatggctc gcggtaatgc cattactttg cctgtatgtg gtcgggatgt gaagtttact
    10741 cttgaagtgc tccggggtga tagtgttgag aagacctctc gggtatggtc aggtaatgaa
    10801 cgtgaccagg agctgcttac tgaggacgca ctggatgatc tcatcccttc ttttctactg
    10861 actggtcaac agacaccggc gttcggtcga agagtatctg gtgtcataga aattgccgat
    10921 gggagtcgcc gtcgtaaagc tgctgcactt accgaaagtg attatcgtgt tctggttggc
    10981 gagctggatg atgagcagat ggctgcatta tccagattgg gtaacgatta tcgcccaaca
    11041 agtgcttatg aacgtggtca gcgttatgca agccgattgc agaatgaatt tgctggaaat
    11101 atttctgcgc tggctgatgc ggaaaatatt tcacgtaaga ttattacccg ctgtatcaac
    11161 accgccaaat tgcctaaatc agttgttgct cttttttctc accccggtga actatctgcc
    11221 cggtcaggtg atgcacttca aaaagccttt acagataaag aggaattact taagcagcag
    11281 gcatctaacc ttcatgagca gaaaaaagct ggggtgatat ttgaagctga agaagttatc
    11341 actcttttaa cttctgtgct taaaacgtca tctgcatcaa gaactagttt aagctcacga
    11401 catcagtttg ctcctggagc gacagtattg tataagggcg ataaaatggt gcttaacctg
    11461 gacaggtctc gtgttccaac tgagtgtata gagaaaattg aggccattct taaggaactt
    11521 gaaaagccag caccctgatg cgaccacgtt ttagtctacg tttatctgtc tttacttaat
    11581 gtcctttgtt acaggccaga aagcataact ggcctgaata ttctctctgg gcccactgtt
    11641 ccacttgtat cgtcggtctg ataatcagac tgggaccacg gtcccactcg tatcgtcggt
    11701 ctgattatta gtctgggacc acggtcccac tcgtatcgtc ggtctgatta ttagtctggg
    11761 accacggtcc cactcgtatc gtcggtctga taatcagact gggaccacgg tcccactcgt
    11821 atcgtcggtc tgattattag tctgggacca tggtcccact cgtatcgtcg gtctgattat
    11881 tagtctggga ccacggtccc actcgtatcg tcggtctgat tattagtctg gaaccacggt
    11941 cccactcgta tcgtcggtct gattattagt ctgggaccac ggtcccactc gtatcgtcgg
    12001 tctgattatt agtctgggac cacgatccca ctcgtgttgt cggtctgatt atcggtctgg
    12061 gaccacggtc ccacttgtat tgtc
//
