LOCUS       40924_14445       12217 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pDEST373-U1Ux, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12217)
  AUTHORS   MacPherson MI, Saka Y.
  TITLE     Short Synthetic Terminators for Assembly of Transcription Units in 
            vitro and Stable Chromosomal Integration in Yeast S. cerevisiae
  JOURNAL   ACS Synth Biol (2016) In press
  PUBMED    27529501
REFERENCE   2  (bases 1 to 12217)
  AUTHORS   Saka Y.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 12217)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12217)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol (2016) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-MAY-2016) School of Medicine, Medical Sciences "
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12217
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    67..851
                     /label=FCY1 YIp-Out
                     /note="FCY1 YIp-Out"
     misc_feature    858..897
                     /label=U1 sequence
                     /note="U1 sequence"
     misc_feature    914..953
                     /label=Ux sequence
                     /note="Ux sequence"
     misc_feature    966..1265
                     /label=FCY1 YIp-In
                     /note="FCY1 YIp-In"
     CDS             1271..1288
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      1355..1402
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(1779..2654)
                     /codon_start=1
                     /label=araC
                     /note="L-arabinose regulatory protein"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        2681..2965
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     CDS             3025..4170
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWLQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMDFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(4344..5000)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(5001..5103)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     promoter        5419..5634
                     /label=URA3 promoter
     CDS             5635..6435
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(7090..7802)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      7881..8100
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             8191..8943
                     /codon_start=1
                     /label=repE
                     /note="replication initiation protein for the bacterial F 
                     plasmid"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    8949..9199
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             9525..10697
                     /codon_start=1
                     /label=sopA
                     /note="partitioning protein for the bacterial F plasmid"
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             10700..11668
                     /codon_start=1
                     /label=sopB
                     /note="partitioning protein for the bacterial F plasmid"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    11744..12217
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
ORIGIN
        1 gatcagacta tcagcgtgag actacgattc catcaatgcc tgtcaagggc aagtattgac
       61 atgtgcgccg acccgggaga tcccatgcta tttgcgtctt cccaagtccc agcgttccag
      121 tattcatact gcgcgttaga gtggtagcca taagagccgg catattggta attttcagta
      181 ttaacgttag aacgtggtga atacgatgtg gtccagcctt gcctcgttgt gtcatatacg
      241 atctttttct ttgggtcaca aagaatatca tatgcttgag agatgacttt aaatctatgt
      301 agtttttcgc ttgatgttag cagcagcggt gatttactat cactgttggt aaccttttct
      361 gagctaaata tttgaatgtt atcggaatgg tcagggtggt acaattttac ataacgatga
      421 tatttttttt ttaacgactt cttgtccagt ttaggatttc cagatccggc ctttggaatg
      481 ccaaaaatat catagggagt tggatctgcc aactcaggcc attgttcatc ccttatcgta
      541 agttttctat tgccattttt atcgttcgct gtagcatact tagctataaa agtgatttgt
      601 gggggacact tttctacaca tgataagtgc cacttgaata aaaatgggta tacgaactta
      661 tggtgtagca taacaaatat attgcaagta gtgacctatg gtgtgtagat atacgtacag
      721 ttagttacga gcctaaagac acaacgtgtt tgttaattat actgtcgctg taatatcttc
      781 tcttccatta tcaccggtca ttccttgcag gggcggtagt acccggagac cctgaacgaa
      841 ttaattctgg cggccgctaa gactttatca acgctcacaa tcgtagagga gtaacttcct
      901 aggtcaacgt acgataagtg atttcaactg agtgctttac gactgctaca acagctaggc
      961 cgcactcgag caattccagc atgtatggta ttccacgctg tgttgtcggt gagaacgtta
     1021 atttcaaaag taagggcgag aaatatttac aaactagagg tcacgaggtt gttgttgttg
     1081 acgatgagag gtgtaaaaag atcatgaaac aatttatcgc tcctcggcgc gccgaggagc
     1141 gatgaaagac ctcaggattg gtttgaagat attggtgagt agagcacgca gcacgctgta
     1201 tttacgtatt taattttata tatttgtgca tacactacta gggaagactt gaaaaaaacc
     1261 tagtctcgag caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga
     1321 agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa
     1381 acgggtcttg aggggttttt tgctgaaagg aggaactata tccggattgg cgaatgggac
     1441 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     1501 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     1561 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     1621 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg gccgccgcaa
     1681 tgcttgcata atgtggcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac
     1741 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca
     1801 ttcacttttt cttcacaacc ggcacgaaac tcgctcgggc tggccccggt gcatttttta
     1861 aatactcgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata
     1921 ggcatccggg tagtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag
     1981 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag
     2041 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg
     2101 tactgacaag cctcgcgtac ccgattatcc atcggcggat ggagcgactc gttaatcgct
     2161 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc
     2221 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc
     2281 gcttcatccg ggcgaaagaa acccgtattg gcaaatattg acggccagtt aagccattca
     2341 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga
     2401 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcag
     2461 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata
     2521 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc
     2581 ggcgttaaac ccgccaccag atgggcgtta aacgagtatc ccggcagcag gggatcattt
     2641 tgcgcttcag ccatactttt catactccca ccattcagag aagaaaccaa ttgtccatat
     2701 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac ccaaccggta
     2761 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt
     2821 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca
     2881 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta
     2941 tcgcaactct ctactgtttc tccatacccg tttttttgga tggagtgaaa ggaggaattc
     3001 ggggcaatcc cgcaaggagg gtgaatgaat cggacgtttg accggaaggc atacaggcaa
     3061 gaactgatcg acgcggggtt ttccgccgag gatgccgaaa ccatcgcaag ccgcaccgtc
     3121 atgcgtgcgc cccgcgaaac cttccagtcc gtcggctcga tggtccagca agctacggcc
     3181 aagatcgagc gcgacagcgt gcaactggct ccccctgccc tgcccgcgcc atcggccgcc
     3241 gtggagcgtt cgcgtcgtct cgaacaggag gcggcaggtt tggcgaagtc gatgaccatc
     3301 gacacgcgag gaactatgac gaccaagaag cgaaaaaccg ccggcgagga cctggcaaaa
     3361 caggtcagcg aggccaagca ggccgcgttg ctgaaacaca cgaagcagca gatcaaggaa
     3421 atgcagcttt ccttgttcga tattgcgccg tggccggaca cgatgcgagc gatgccaaac
     3481 gacacggccc gctctgccct gttcaccacg cgcaacaaga aaatcccgcg cgaggcgctg
     3541 caaaacaagg tcattttcca cgtcaacaag gacgtgaaga tcacctacac cggcgtcgag
     3601 ctgcgggccg acgatgacga actggtgtgg ctgcaggtgt tggagtacgc gaagcgcacc
     3661 cctatcggcg agccgatcac cttcacgttc tacgagcttt gccaggacct gggctggtcg
     3721 atcaatggcc ggtattacac gaaggccgag gaatgcctgt cgcgcctaca ggcgacggcg
     3781 atggacttca cgtccgaccg cgttgggcac ctggaatcgg tgtcgctgct gcaccgcttc
     3841 cgcgtcctgg accgtggcaa gaaaacgtcc cgttgccagg tcctgatcga cgaggaaatc
     3901 gtcgtgctgt ttgctggcga ccactacacg aaattcatat gggagaagta ccgcaagctg
     3961 tcgccgacgg cccgacggat gttcgactat ttcagctcgc accgggagcc gtacccgctc
     4021 aagctggaaa ccttccgcct catgtgcgga tcggattcca cccgcgtgaa gaagtggcgc
     4081 gagcaggtcg gcgaagcctg cgaagagttg cgaggcagcg gcctggtgga acacgcctgg
     4141 gtcaatgatg acctggtgca ttgcaaacgc tagggccttg tggggtcagt tccggctggg
     4201 ggttcagcag ccaccgggcc gtcgaccaat tctcatgttt gacagcttat catcgaattt
     4261 ctgccattca tccgcttatt atcacttatt caggcgtagc aaccaggcgt ttaagggcac
     4321 caataactgc cttaaaaaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat
     4381 tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc
     4441 agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg
     4501 aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg
     4561 gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg
     4621 taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca
     4681 ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca
     4741 ctatcccata tcaccagctc accgtctttc attgccatac ggaattccgg atgagcattc
     4801 atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg
     4861 gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact
     4921 gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat
     4981 ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa
     5041 aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgccga
     5101 tcaacgtctc attttcgcca aaagttggcc cagggcttcc cggtatcaac agggacacca
     5161 ggatttattt attctgcgaa gtgatcttcc gtcacaggta tttattcgcg ataagctcat
     5221 ggagcggcgt aaccgtcgca caggaaggac agagaaagcg cggatctggg aagtgacgga
     5281 cagaacggtc aggacctgga ttggggaggc ggttgccgcc gctgctgctg acggtgtgac
     5341 gttctctgtt ccggtcacac cacatacgtt ccgccattcc tatgcgatgc acatgctgta
     5401 tgccggtaac cacagctttt caattcatca tttttttttt attctttttt ttgatttcgg
     5461 tttccttgaa atttttttga ttcggtaatc tccgaacaga aggaagaacg aaggaaggag
     5521 cacagactta gattggtata tatacgcata tgtagtgttg aagaaacatg aaattgccca
     5581 gtattcttaa cccaactgca cagaacaaaa acctgcagga aacgaagata aatcatgtcg
     5641 aaagctacat ataaggaacg tgctgctact catcctagtc ctgttgctgc caagctattt
     5701 aatatcatgc acgaaaagca aacaaacttg tgtgcttcat tggatgttcg taccaccaag
     5761 gaattactgg agttagttga agcattaggt cccaaaattt gtttactaaa aacacatgtg
     5821 gatatcttga ctgatttttc catggagggc acagttaagc cgctaaaggc attatccgcc
     5881 aagtacaatt ttttactctt cgaagacaga aaatttgctg acattggtaa tacagtcaaa
     5941 ttgcagtact ctgcgggtgt atacagaata gcagaatggg cagacattac gaatgcacac
     6001 ggtgtggtgg gcccaggtat tgttagcggt ttgaagcagg cggcagaaga agtaacaaag
     6061 gaacctagag gccttttgat gttagcagaa ttgtcatgca agggctccct atctactgga
     6121 gaatatacta agggtactgt tgacattgcg aagagcgaca aagattttgt tatcggcttt
     6181 attgctcaaa gagacatggg tggaagagat gaaggttacg attggttgat tatgacaccc
     6241 ggtgtgggtt tagatgacaa gggagacgca ttgggtcaac agtatagaac cgtggatgat
     6301 gtggtctcta caggatctga cattattatt gttggaagag gactatttgc aaagggaagg
     6361 gatgctaagg tagagggtga acgttacaga aaagcaggct gggaagcata tttgagaaga
     6421 tgcggccagc aaaactaaaa aactgtatta taagtaaatg catgtatact aaactcacaa
     6481 attagagctt caatttaatt atatcagtta ttaccctatg cggtgtgaaa ttaccgctga
     6541 aagttctgca aagcctgatg ggacataagt ccatcagttc aacggaagtc tacacgaagg
     6601 tttttgcgct ggatgtggct gcccggcacc gggtgcagtt tgcgatgccg gagtctgatg
     6661 cggttgcgat gctgaaacaa ttatcctgag aataaatgcc ttggccttta tatggaaatg
     6721 tggaactgag tggatatgct gtttttgtct gttaaacaga gaagctggct gttatccact
     6781 gagaagcgaa cgaaacagtc gggaaaatct cccattatcg tagagatccg cattattaat
     6841 ctcaggagcc tgtgtagcgt ttataggaag tagtgttctg tcatgatgcc tgcaagcggt
     6901 aacgaaaacg atttgaatat gccttcagga acaatagaaa tcttcgtgcg gtgttacgtt
     6961 gaagtggagc ggattatgtc agcaatggac agaacaacct aatgaacaca gaaccatgat
     7021 gtggtctgtc cttttacagc cagtagtgct cgccgcagtc gagcgacagg gcgaagccct
     7081 cgagatcccc tgccattttt ggggtgaggc cgttcgcggc cgaggggcgc agcccctggg
     7141 gggatgggag gcccgcgtta gcggccggga gggttcgaga agggggggca ccccccttcg
     7201 gcgtgcgcgg tcacgcgcac agggcgcagc cctggttaaa aacaaggttt ataaatattg
     7261 gtttaaaagc aggttaaaag acaggttagc ggtggccgaa aaacgggcgg aaacccttgc
     7321 aaatgctgga ttttctgcct gtggacagcc cctcaaatgt caataggtgc gcccctcatc
     7381 tgtcagcact ctgcccctca agtgtcaagg atcgcgcccc tcatctgtca gtagtcgcgc
     7441 ccctcaagtg tcaataccgc agggcactta tccccaggct tgtccacatc atctgtggga
     7501 aactcgcgta aaatcaggcg ttttcgccga tttgcgaggc tggccagctc cacgtcgccg
     7561 gccgaaatcg agcctgcccc tcatctgtca acgccgcgcc gggtgagtcg gcccctcaag
     7621 tgtcaacgtc cgcccctcat ctgtcagtga gggccaagtt ttccgcgagg tatccacaac
     7681 gccggcggcc ggccgcggtg tctcgcacac ggcttcgacg gcgtttctgg cgcgtttgca
     7741 gggccataga cggccgccag cccagcggcg agggcaacca gcccggtgag cgtcggaaag
     7801 gggaatttcg agtgagcgag gaagcaccag ggaacagcac ttatatattc tgcttacaca
     7861 cgatgcctga aaaaacttcc cttggggtta tccacttatc cacggggata tttttataat
     7921 tatttttttt atagttttta gatcttcttt tttagagcgc cttgtaggcc tttatccatg
     7981 ctggttctag agaaggtgtt gtgacaaatt gccctttcag tgtgacaaat caccctcaaa
     8041 tgacagtcct gtctgtgaca aattgccctt aaccctgtga caaattgccc tcagaagaag
     8101 ctgttttttc acaaagttat ccctgcttat tgactctttt ttatttagtg tgacaatcta
     8161 aaaacttgtc acacttcaca tggatctgtc atggcggaaa cagcggttat caatcacaag
     8221 aaacgtaaaa atagcccgcg aatcgtccag tcaaacgacc tcactgaggc ggcatatagt
     8281 ctctcccggg atcaaaaacg tatgctgtat ctgttcgttg accagatcag aaaatctgat
     8341 ggcaccctac aggaacatga cggtatctgc gagatccatg ttgctaaata tgctgaaata
     8401 ttcggattga cctctgcgga agccagtaag gatatacggc aggcattgaa gagtttcgcg
     8461 gggaaggaag tggtttttta tcgccctgaa gaggatgccg gcgatgaaaa aggctatgaa
     8521 tcttttcctt ggtttatcaa acgtgcgcac agtccatcca gagggcttta cagtgtacat
     8581 atcaacccat atctcattcc cttctttatc gggttacaga accggtttac gcagtttcgg
     8641 cttagtgaaa caaaagaaat caccaatccg tatgccatgc gtttatacga atccctgtgt
     8701 cagtatcgta agccggatgg ctcaggcatc gtctctctga aaatcgactg gatcatagag
     8761 cgttaccagc tgcctcaaag ttaccagcgt atgcctgact tccgccgccg cttcctgcag
     8821 gtctgtgtta atgagatcaa cagcagaact ccaatgcgcc tctcatacat tgagaaaaag
     8881 aaaggccgcc agacgactca tatcgtattt tccttccgcg atatcacttc catgacgaca
     8941 ggatagtctg agggttatct gtcacagatt tgagggtggt tcgtcacatt tgttctgacc
     9001 tactgagggt aatttgtcac agttttgctg tttccttcag cctgcatgga ttttctcata
     9061 ctttttgaac tgtaattttt aaggaagcca aatttgaggg cagtttgtca cagttgattt
     9121 ccttctcttt cccttcgtca tgtgacctga tatcgggggt tagttcgtca tcattgatga
     9181 gggttgatta tcacagttta ttactctgaa ttggctatcc gcgtgtgtac ctctacctgg
     9241 agtttttccc acggtggata tttcttcttg cgctgagcgt aagagctatc tgacagaaca
     9301 gttcttcttt gcttcctcgc cagttcgctc gctatgctcg gttacacggc tgcggcgagc
     9361 gctagtgata ataagtgact gaggtatgtg ctcttcttat ctccttttgt agtgttgctc
     9421 ttattttaaa caactttgcg gttttttgat gactttgcga ttttgttgtt gctttgcagt
     9481 aaattgcaag atttaataaa aaaacgcaaa gcaatgatta aaggatgttc agaatgaaac
     9541 tcatggaaac acttaaccag tgcataaacg ctggtcatga aatgacgaag gctatcgcca
     9601 ttgcacagtt taatgatgac agcccggaag cgaggaaaat aacccggcgc tggagaatag
     9661 gtgaagcagc ggatttagtt ggggtttctt ctcaggctat cagagatgcc gagaaagcag
     9721 ggcgactacc gcacccggat atggaaattc gaggacgggt tgagcaacgt gttggttata
     9781 caattgaaca aattaatcat atgcgtgatg tgtttggtac gcgattgcga cgtgctgaag
     9841 acgtatttcc accggtgatc ggggttgctg cccataaagg tggcgtttac aaaacctcag
     9901 tttctgttca tcttgctcag gatctggctc tgaaggggct acgtgttttg ctcgtggaag
     9961 gtaacgaccc ccagggaaca gcctcaatgt atcacggatg ggtaccagat cttcatattc
    10021 atgcagaaga cactctcctg cctttctatc ttggggaaaa ggacgatgtc acttatgcaa
    10081 taaagcccac ttgctggccg gggcttgaca ttattccttc ctgtctggct ctgcaccgta
    10141 ttgaaactga gttaatgggc aaatttgatg aaggtaaact gcccaccgat ccacacctga
    10201 tgctccgact ggccattgaa actgttgctc atgactatga tgtcatagtt attgacagcg
    10261 cgcctaacct gggtatcggc acgattaatg tcgtatgtgc tgctgatgtg ctgattgttc
    10321 ccacgcctgc tgagttgttt gactacacct ccgcactgca gtttttcgat atgcttcgtg
    10381 atctgctcaa gaacgttgat cttaaagggt tcgagcctga tgtacgtatt ttgcttacca
    10441 aatacagcaa tagtaatggc tctcagtccc cgtggatgga ggagcaaatt cgggatgcct
    10501 ggggaagcat ggttctaaaa aatgttgtac gtgaaacgga tgaagttggt aaaggtcaga
    10561 tccggatgag aactgttttt gaacaggcca ttgatcaacg ctcttcaact ggtgcctgga
    10621 gaaatgctct ttctatttgg gaacctgtct gcaatgaaat tttcgatcgt ctgattaaac
    10681 cacgctggga gattagataa tgaagcgtgc gcctgttatt ccaaaacata cgctcaatac
    10741 tcaaccggtt gaagatactt cgttatcgac accagctgcc ccgatggtgg attcgttaat
    10801 tgcgcgcgta ggagtaatgg ctcgcggtaa tgccattact ttgcctgtat gtggtcggga
    10861 tgtgaagttt actcttgaag tgctccgggg tgatagtgtt gagaagacct ctcgggtatg
    10921 gtcaggtaat gaacgtgacc aggagctgct tactgaggac gcactggatg atctcatccc
    10981 ttcttttcta ctgactggtc aacagacacc ggcgttcggt cgaagagtat ctggtgtcat
    11041 agaaattgcc gatgggagtc gccgtcgtaa agctgctgca cttaccgaaa gtgattatcg
    11101 tgttctggtt ggcgagctgg atgatgagca gatggctgca ttatccagat tgggtaacga
    11161 ttatcgccca acaagtgctt atgaacgtgg tcagcgttat gcaagccgat tgcagaatga
    11221 atttgctgga aatatttctg cgctggctga tgcggaaaat atttcacgta agattattac
    11281 ccgctgtatc aacaccgcca aattgcctaa atcagttgtt gctctttttt ctcaccccgg
    11341 tgaactatct gcccggtcag gtgatgcact tcaaaaagcc tttacagata aagaggaatt
    11401 acttaagcag caggcatcta accttcatga gcagaaaaaa gctggggtga tatttgaagc
    11461 tgaagaagtt atcactcttt taacttctgt gcttaaaacg tcatctgcat caagaactag
    11521 tttaagctca cgacatcagt ttgctcctgg agcgacagta ttgtataagg gcgataaaat
    11581 ggtgcttaac ctggacaggt ctcgtgttcc aactgagtgt atagagaaaa ttgaggccat
    11641 tcttaaggaa cttgaaaagc cagcaccctg atgcgaccac gttttagtct acgtttatct
    11701 gtctttactt aatgtccttt gttacaggcc agaaagcata actggcctga atattctctc
    11761 tgggcccact gttccacttg tatcgtcggt ctgataatca gactgggacc acggtcccac
    11821 tcgtatcgtc ggtctgatta ttagtctggg accacggtcc cactcgtatc gtcggtctga
    11881 ttattagtct gggaccacgg tcccactcgt atcgtcggtc tgataatcag actgggacca
    11941 cggtcccact cgtatcgtcg gtctgattat tagtctggga ccatggtccc actcgtatcg
    12001 tcggtctgat tattagtctg ggaccacggt cccactcgta tcgtcggtct gattattagt
    12061 ctggaaccac ggtcccactc gtatcgtcgg tctgattatt agtctgggac cacggtccca
    12121 ctcgtatcgt cggtctgatt attagtctgg gaccacgatc ccactcgtgt tgtcggtctg
    12181 attatcggtc tgggaccacg gtcccacttg tattgtc
//
