LOCUS       Exported               12266 bp ds-DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12266)
  AUTHORS   caoheibi
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..12266
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        849..1553
                     /gene="S. cerevisiae ADH1"
                     /note="ADH1 promoter"
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             1581..2021
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /note="GAL4 DNA binding domain"
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     protein_bind    2037..2161
                     /gene="mutant version of attR"
                     /bound_moiety="LR Clonase(TM)"
                     /note="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2308..2410
                     /note="cat promoter"
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2411..3091
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     CDS             3411..3716
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /note="ccdB"
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(3757..3881)
                     /gene="mutant version of attR"
                     /bound_moiety="LR Clonase(TM)"
                     /note="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      4081..4268
                     /gene="S. cerevisiae ADH1"
                     /note="ADH1 terminator"
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     promoter        complement(4421..4439)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4446..4462)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      4603..5058
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5359..5766
                     /gene="S. cerevisiae LEU2"
                     /note="LEU2 promoter"
     CDS             5767..6861
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /note="LEU2"
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     misc_feature    7594..8097
                     /note="CEN/ARS"
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     CDS             complement(8452..8985)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /note="GmR"
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(9174..9202)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /note="Pc promoter"
                     /note="class 1 integron promoter"
     rep_origin      9859..10447
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    10735..10756
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10771..10801
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    10809..10825
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10833..10849
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        10870..10888
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      10918..11165
                     /gene="S. cerevisiae CYC1"
                     /note="CYC1 terminator"
                     /note="transcription terminator for CYC1"
ORIGIN
        1 tatctctatg taagctacgt ggcgtgactt ttgatgaagc cgcacaagag atacaggatt
       61 ggcaactgca aatagaatct ggggatcccc cctcgagatc cgggatcgaa gaaatgatgg
      121 taaatgaaat aggaaatcaa ggagcatgaa ggcaaaagac aaatataagg gtcgaacgaa
      181 aaataaagtg aaaagtgttg atatgatgta tttggctttg cggcgccgaa aaaacgagtt
      241 tacgcaattg cacaatcatg ctgactctgt ggcggacccg cgctcttgcc ggcccggcga
      301 taacgctggg cgtgaggctg tgcccggcgg agttttttgc gcctgcattt tccaaggttt
      361 accctgcgct aaggggcgag attggagaag caataagaat gccggttggg gttgcgatga
      421 tgacgaccac gacaactggt gtcattattt aagttgccga aagaacctga gtgcatttgc
      481 aacatgagta tactagaaga atgagccaag acttgcgaga cgcgagtttg ccggtggtgc
      541 gaacaataga gcgaccatga ccttgaaggt gagacgcgca taaccgctag agtactttga
      601 agaggaaaca gcaatagggt tgctaccagt ataaatagac aggtacatac aacactggaa
      661 atggttgtct gtttgagtac gctttcaatt catttgggtg tgcactttat tatgttacaa
      721 tatggaaggg aactttacac ttctcctatg cacatatatt aattaaagtc caatgctagt
      781 agagaagggg ggtaacaccc ctccgcgctc ttttccgatt tttttctaaa ccgtggaata
      841 tttcggatat ccttttgttg tttccgggtg tacaatatgg acttcctctt ttctggcaac
      901 caaacccata catcgggatt cctataatac cttcgttggt ctccctaaca tgtaggtggc
      961 ggaggggaga tatacaatag aacagatacc agacaagaca taatgggcta aacaagacta
     1021 caccaattac actgcctcat tgatggtggt acataacgaa ctaatactgt agccctagac
     1081 ttgatagcca tcatcatatc gaagtttcac tacccttttt ccatttgcca tctattgaag
     1141 taataatagg cgcatgcaac ttcttttctt tttttttctt ttctctctcc cccgttgttg
     1201 tctcaccata tccgcaatga caaaaaaatg atggaagaca ctaaaggaaa aaattaacga
     1261 caaagacagc accaacagat gtcgttgttc cagagctgat gaggggtatc tcgaagcaca
     1321 cgaaactttt tccttccttc attcacgcac actactctct aatgagcaac ggtatacggc
     1381 cttccttcca gttacttgaa tttgaaataa aaaaaagttt gctgtcttgc tatcaagtat
     1441 aaatagacct gcaattatta atcttttgtt tcctcgtcat tgttctcgtt ccctttcttc
     1501 cttgtttctt tttctgcaca atatttcaag ctataccaag catacaatca actccaagct
     1561 tgaagcaagc ctcctgaaag atgaagctac tgtcttctat cgaacaagca tgcgatattt
     1621 gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa gtgcgccaag tgtctgaaga
     1681 acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc tccgctgact agggcacatc
     1741 tgacagaagt ggaatcaagg ctagaaagac tggaacagct atttctactg atttttcctc
     1801 gagaagacct tgacatgatt ttgaaaatgg attctttaca ggatataaaa gcattgttaa
     1861 caggattatt tgtacaagat aatgtgaata aagatgccgt cacagataga ttggcttcag
     1921 tggagactga tatgcctcta acattgagac agcatagaat aagtgcgaca tcatcatcgg
     1981 aagagagtag taacaaaggt caaagacagt tgactgtatc gtcgaggtcg aatcaaacaa
     2041 gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt
     2101 agattttgca taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat
     2161 ggcggccgcg ggtgagtcag catcacccga cgcactttgc gccgaataaa tacctgtgac
     2221 ggaagatcac ttcgcagaat aaataaatcc tggtgtccct gttgataccg ggaagccctg
     2281 ggccaacttt tggcgaaaat gagacgttga tcggcacgta agaggttcca actttcacca
     2341 taatgaaata agatcactac cgggcgtatt ttttgagtca tcgagatttt caggagctaa
     2401 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
     2461 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
     2521 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
     2581 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat
     2641 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
     2701 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
     2761 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
     2821 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga
     2881 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta
     2941 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     3001 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggc
     3061 ggggcgtaat ctagaggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc
     3121 gctgattttt gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga
     3181 ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca
     3241 aggcatatat gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg
     3301 tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg
     3361 gtttattgaa atgaacggct cttttgctga cgagaacagg ggctggtgaa atgcagttta
     3421 aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata
     3481 ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag
     3541 ataaagtccc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga
     3601 tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca
     3661 gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt
     3721 caggctccct tatacacagc cagtctgcag gtcgaccata gtgactggat atgttgtgtt
     3781 ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata
     3841 tcattttacg tttctcgttc agctttcttg tacaaagtgg tttgatggcc gctaagtaag
     3901 taagacgtcg agctctaagt aagtaacggc cgccaccgcg gtggagcttt ggacttcttc
     3961 gccagaggtt tggtcaagtc tccaatcaag gttgtcggct tgtctacctt gccagaaatt
     4021 tacgaaaaga tggaaaaggg tcaaatcgtt ggtagatacg ttgttgacac ttctaaataa
     4081 gcgaatttct tatgatttat gatttttatt attaaataag ttataaaaaa aataagtgta
     4141 tacaaatttt aaagtgactc ttaggtttta aaacgaaaat tcttattctt gagtaactct
     4201 ttcctgtagg tcaggttgct ttctcaggta tagcatgagg tcgctcttat tgaccacacc
     4261 tctaccggca tgccgagcaa atgcctgcaa atcgctcccc atttcaccca attgtagata
     4321 tgctaactcc agcaatgagt tgatgaatct cggtgtgtat tttatgtcct cagaggacaa
     4381 cacctgttgt aatcgttctt ccacacggat cccaattcgc cctatagtga gtcgtattac
     4441 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     4501 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     4561 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg
     4621 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     4681 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     4741 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     4801 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     4861 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     4921 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     4981 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     5041 tattaacgct tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     5101 acaccgcata tcgaccggtc gaggagaact tctagtatat ctacatacct aatattattg
     5161 ccttattaaa aatggaatcc caacaattac atcaaaatcc acattctctt caaaatcaat
     5221 tgtcctgtac ttccttgttc atgtgtgttc aaaaacgtta tatttatagg ataattatac
     5281 tctatttctc aacaagtaat tggttgtttg gccgagcggt ctaaggcgcc tgattcaaga
     5341 aatatcttga ccgcagttaa ctgtgggaat actcaggtat cgtaagatgc aagagttcga
     5401 atctcttagc aaccattatt tttttcctca acataacgag aacacacagg ggcgctatcg
     5461 cacagaatca aattcgatga ctggaaattt tttgttaatt tcagaggtcg cctgacgcat
     5521 ataccttttt caactgaaaa attgggagaa aaaggaaagg tgagagcgcc ggaaccggct
     5581 tttcatatag aatagagaag cgttcatgac taaatgcttg catcacaata cttgaagttg
     5641 acaatattat ttaaggacct attgtttttt ccaataggtg gttagcaatc gtcttacttt
     5701 ctaacttttc ttacctttta catttcagca atatatatat atatatttca aggatatacc
     5761 attctaatgt ctgcccctaa gaagatcgtc gttttgccag gtgaccacgt tggtcaagaa
     5821 atcacagccg aagccattaa ggttcttaaa gctatttctg atgttcgttc caatgtcaag
     5881 ttcgatttcg aaaatcattt aattggtggt gctgctatcg atgctacagg tgttccactt
     5941 ccagatgagg cgctggaagc ctccaagaag gctgatgccg ttttgttagg tgctgtgggt
     6001 ggtcctaaat ggggtaccgg tagtgttaga cctgaacaag gtttactaaa aatccgtaaa
     6061 gaacttcaat tgtacgccaa cttaagacca tgtaactttg catccgactc tcttttagac
     6121 ttatctccaa tcaagccaca atttgctaaa ggtactgact tcgttgttgt cagagaatta
     6181 gtgggaggta tttactttgg taagagaaag gaagacgatg gtgatggtgt cgcttgggat
     6241 agtgaacaat acaccgttcc agaagtgcaa agaatcacaa gaatggccgc tttcatggcc
     6301 ctacaacatg agccaccatt gcctatttgg tccttggata aagctaatgt tttggcctct
     6361 tcaagattat ggagaaaaac tgtggaggaa accatcaaga acgaattccc tacattgaag
     6421 gttcaacatc aattgattga ttctgccgcc atgatcctag ttaagaaccc aacccaccta
     6481 aatggtatta taatcaccag caacatgttt ggtgatatca tctccgatga agcctccgtt
     6541 atcccaggtt ccttgggttt gttgccatct gcgtccttgg cctctttgcc agacaagaac
     6601 accgcatttg gtttgtacga accatgccac ggttctgctc cagatttgcc aaagaataag
     6661 gtcaacccta tcgccactat cttgtctgct gcaatgatgt tgaaattgtc attgaacttg
     6721 cctgaagaag gtaaggccat tgaagatgca gttaaaaagg ttttggatgc aggtatcaga
     6781 actggtgatt taggtggttc caacagtacc accgaagtcg gtgatgctgt cgccgaagaa
     6841 gttaagaaaa tccttgctta aaaagattct ctttttttat gatatttgta cataaacttt
     6901 ataaatgaaa ttcataatag aaacgacacg aaattacaaa atggaatatg ttcatagggt
     6961 agacgaaact atatacgcaa tctacataca tttatcaaga aggagaaaaa ggaggatgta
     7021 aaggaataca ggtaagcaaa ttgatactaa tggctcaacg tgataaggaa aaagaattgc
     7081 actttaacat taatattgac aaggaggagg gcaccacaca aaaagttagg tgtaacagaa
     7141 aatcatgaaa ctatgattcc taatttatat attggaggat tttctctaaa aaaaaaaaaa
     7201 tacaacaaat aaaaaacact caatgacctg accatttgat ggagtttaag tcaatacctt
     7261 cttgaaccat ttcccataat ggtgaaagtt ccctcaagaa ttttactctg tcagaaacgg
     7321 ccttaacgac gtagtcgata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     7381 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     7441 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     7501 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     7561 ttaatgtcat gataataatg gtttcttagg acggatcgct tgcctgtaac ttacacgcgc
     7621 ctcgtatctt ttaatgatgg aataatttgg gaatttactc tgtgtttatt tatttttatg
     7681 ttttgtattt ggattttaga aagtaaataa agaaggtaga agagttacgg aatgaagaaa
     7741 aaaaaataaa caaaggttta aaaaatttca acaaaaagcg tactttacat atatatttat
     7801 tagacaagaa aagcagatta aatagatata cattcgatta acgataagta aaatgtaaaa
     7861 tcacaggatt ttcgtgtgtg gtcttctaca cagacaagat gaaacaattc ggcattaata
     7921 cctgagagca ggaagagcaa gataaaaggt agtatttgtt ggcgatcccc ctagagtctt
     7981 ttacatcttc ggaaaacaaa aactattttt tctttaattt ctttttttac tttctatttt
     8041 taatttatat atttatatta aaaaatttaa attataatta tttttatagc acgtgatgaa
     8101 aaggacccag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     8161 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     8221 tctgcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
     8281 atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag
     8341 ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata
     8401 tgggtataaa tgggctcggg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc
     8461 ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag
     8521 agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc
     8581 gttggcctca tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc
     8641 gccggagact gcgagatcat agatatagat ctcactacgc ggctgctcaa acttgggcag
     8701 aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa
     8761 tgtcttacta cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt
     8821 ggctacgtct ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg
     8881 gtcagggccg agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt
     8941 agggcgactg ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata
     9001 acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact
     9061 gtacaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc
     9121 gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac
     9181 gaaccgaaca ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc
     9241 acccggcaac cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc
     9301 gcaaggtttc ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca
     9361 aggtgctgtg cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc
     9421 gcttgccggt ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg
     9481 agcatcgttt gttcgcccag gactctagct atagttctag tggttggcta ctaataggtt
     9541 gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga
     9601 actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg
     9661 ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag
     9721 aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa
     9781 gctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     9841 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     9901 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     9961 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
    10021 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
    10081 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
    10141 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
    10201 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
    10261 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
    10321 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
    10381 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
    10441 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
    10501 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
    10561 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
    10621 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
    10681 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
    10741 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
    10801 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
    10861 caagctcgaa attaaccctc actaaaggga acaaaagctg gtaccgatcc cgagcttgca
    10921 aattaaagcc ttcgagcgtc ccaaaacctt ctcaagcaag gttttcagta taatgttaca
    10981 tgcgtacacg cgtctgtaca gaaaaaaaag aaaaatttga aatataaata acgttcttaa
    11041 tactaacata actataaaaa aataaatagg gacctagact tcaggttgtc taactccttc
    11101 cttttcggtt agagcggatg tggggggagg gcgtgaatgt aagcgtgaca taactaatta
    11161 catgatatcg acaaaggaaa aggggcctgt ttactcacag gcttttttca agtaggtaat
    11221 taagtcgttt ctgtcttttt ccttcttcaa cccaccaaag gccatcttgg tacttttttt
    11281 tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
    11341 tttttttttt ttttgataga aataatacag aagtagatgt tgaattagat taaactgaag
    11401 atatataatt tattggaaaa tacatagagt ttttgttgat gcgcttaagc gatcaattca
    11461 acaacaccac cagcagctct gattttttct tcagccaact tggagacgaa tctagctttg
    11521 acgataactg gaacatttgg aattctaccc ttacccaaga tcttaccgta accggctgcc
    11581 aaagtgtcaa taactggagc agtttcctta gaagcagatt tcaagtattg gtctctcttg
    11641 tcttctggga tcaatgtcca caatttgtcc aagttcaaga ctggcttcca gaaatgagct
    11701 tgttgcttgt ggaagtatct cataccaacc ttaccgaaat aacctggatg gtatttatcc
    11761 atgttaattc tgtggtgatg ttgaccaccg gccatacctc taccaccggg gtgctttctg
    11821 tgcttaccga tacgaccttt accggctgag acgtgacctc tgtgctttct agtcttagtg
    11881 aatctggaag gcattcttga ttagttggat gattgttctg ggatttaatg caaaaaaatc
    11941 actaagaagg aaaaaaatca acggagaaag caaacgccat cttaaatata cgggatacag
    12001 atgaaaggtt tgaacctatc tgggaaaata cgcattaaac aagcgaaaaa ctgcgaggaa
    12061 aattgtttgc gtctctgcgg gctattcacg cgccagagga aaaataggaa aaataacagg
    12121 cattagaaaa ataattttga ttttggtaat gtgtgggtcc ctggtgtaca gatgttacat
    12181 tggttacagt actcttgttt ttgctgtgtt tttcgatgaa tctccaaaat ggttgttagc
    12241 acatggaaga gtcaccgatg ctaagt
//
