LOCUS       V016507                 5842 bp    DNA     circular SYN 12-DEC-2019
DEFINITION  Exported.
ACCESSION   V016507
VERSION     V016507
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5842)
  AUTHORS   Hartley CJ, Williams CC, Scoble JA, Churches QI, North A, French NG,
            Nebl T, Coia G, Warden AC, Simpson G, Frazer AR, Nixon Jensen C,
            Turner NJ, Scott C.
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5842)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (10-MAY-2019) Biocatalysis "
FEATURES             Location/Qualifiers
     source          1..5842
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..39
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             71..93
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             113..130
                     /label="6xHis"
                     /note="6xHis affinity tag"
     protein_bind    140..164
                     /label="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             170..1297
                     /gene="ackA"
                     /label="Acetate kinase"
                     /note="Acetate kinase from Mycolicibacterium smegmatis
                     (strain ATCC 700084 / mc(2)155). Accession#: A0QQK1"
     protein_bind    complement(1308..1332)
                     /label="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      1399..1446
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     promoter        complement(1812..1840)
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     promoter        1953..2057
                     /label="AmpR promoter"
     CDS             2058..2915
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3089..3677
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(3863..4005)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(4110..4298)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
ORIGIN
        1 agatctcgat cccgcgaaat taatacgact cactataggg agaccacaac ggtttccctc
       61 tagaaataat tttgtttaac tttaagaagg agatatacat atgtcgtact accatcacca
      121 tcaccatcac ctcgaatcaa caagtttgta caaaaaagca ggcttccata tgaccgttct
      181 ggttgttaat agcggtagca gcagcctgaa atatgcagtt gttcgtccgg caagcggtga
      241 atttctggca gatggtatta ttgaagaaat tggtagcggt gcagttccgg atcatgatgc
      301 agcactgcgt gcagcatttg atgaactggc agcagcaggt ctgcatctgg aagatctgga
      361 tctgaaagca gttggtcatc gtatggttca tggtggtaaa accttctata aaccgagcgt
      421 tgttgatgat gagctgattg caaaagcacg tgaactgagt ccgctggcac cgctgcataa
      481 tccgcctgca attaaaggta ttgaagttgc acgtaaactg ctgccggatc tgccgcatat
      541 tgcagttttt gataccgcct tttttcatga tctgcctgca ccggcaagca cctatgcaat
      601 tgatcgtgaa ctggccgaaa cctggcatat taaacgttat ggttttcatg gcaccagcca
      661 tgaatatgtt agccagcagg cagcaatttt tctggatcgt ccgctggaaa gcctgaatca
      721 gattgttctg cacctgggta atggtgcaag cgcaagcgca gttgccggtg gtaaagccgt
      781 tgataccagc atgggtctga ccccgatgga aggtctggtt atgggcaccc gtagcggtga
      841 tattgatccg ggtgttatta tgtatctgtg gcgtaccgca ggtatgagcg ttgatgatat
      901 tgaaagcatg ctgaatcgtc gtagtggtgt tctgggtctg ggtggtgcga gcgattttcg
      961 caaactgcgc gaactgattg aaagcggtga tgaacatgca aaactggcct atgatgtgta
     1021 tattcatcgt ctgcgcaaat atatcggtgc atatatggca gtgctgggtc gtaccgatgt
     1081 tattagcttt accgcaggcg ttggtgaaaa tgttccgcct gttcgtcgtg atgccctggc
     1141 aggcctgggt ggtctgggta ttgaaattga tgatgcactg aatagcgcaa aaagtgatga
     1201 accgcgtctg attagcacac cggatagccg tgttacagtt ctggtggttc cgaccaatga
     1261 agaactggca attgcacgtg cctgtgttgg tgttgtttaa gaattcgacc cagctttctt
     1321 gtacaaagtg gttgattcga ggctgctaac aaagcccgaa aggaagctga gttggctgct
     1381 gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt
     1441 tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat
     1501 cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg
     1561 gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag
     1621 cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg
     1681 cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac
     1741 gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga
     1801 taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac
     1861 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     1921 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     1981 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     2041 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     2101 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     2161 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     2221 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     2281 gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact
     2341 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     2401 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     2461 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     2521 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     2581 agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     2641 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     2701 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     2761 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     2821 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     2881 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     2941 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     3001 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     3061 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     3121 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     3181 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     3241 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3301 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     3361 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     3421 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     3481 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     3541 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     3601 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     3661 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     3721 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     3781 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     3841 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     3901 tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     3961 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca
     4021 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     4081 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     4141 aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt
     4201 tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag
     4261 cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga
     4321 tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt
     4381 actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg
     4441 atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat
     4501 gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg
     4561 cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat
     4621 gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc
     4681 ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac
     4741 aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg
     4801 cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc
     4861 acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg
     4921 tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg
     4981 ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg
     5041 ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa
     5101 gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca
     5161 tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg
     5221 ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc
     5281 cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt
     5341 gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc
     5401 agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt
     5461 gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga
     5521 aggagctgac tgggttgaag gctctcaagg gcatcggtcg atcgacgctc tcccttatgc
     5581 gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca
     5641 aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc
     5701 atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg
     5761 gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg
     5821 atgcgtccgg cgtagaggat cg
//
