LOCUS       40924_14425       11519 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pDEST152, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11519)
  AUTHORS   Wu FL, Liu Y, Zhang HN, Jiang HW, Cheng L, Guo SJ, Deng JY, Bi LJ, 
            Zhang XE, Gao HF, Tao SC.
  TITLE     Global profiling of PknG interactions using a human proteome 
            microarray reveals novel connections with CypA
  JOURNAL   Proteomics, e1800265 (2018) In press
  PUBMED    30281201
REFERENCE   2  (bases 1 to 11519)
  AUTHORS   Wu FL, Tao SC.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2018) Shanghai Center for Systems Biomedicine, 
            Shanghai Jiao Tong University, Dongchuan Road, No. 800, Shanghai, 
            Shanghai 200210, China
REFERENCE   3  (bases 1 to 11519)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11519)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proteomics,
            e1800265 (2018) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-MAR-2018) Shanghai Center for Systems Biomedicine, Shanghai Jiao
            Tong University, Dongchuan Road, No. 800, Shanghai, Shanghai 200210,
            China"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11519
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      475..2264
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(2573..4495)
                     /codon_start=1
                     /label=EBNA1
                     /note="Epstein-Barr nuclear antigen 1, also known as
                     EBNA-1"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     promoter        5016..5120
                     /label=AmpR promoter
     CDS             5121..5978
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6152..6740
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        6968..7265
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             7343..7939
                     /codon_start=1
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     polyA_signal    8033..8167
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        8426..8805
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8806..9009
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             9178..9195
                     /codon_start=1
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
                     /translation="LVPRGS"
     protein_bind    9208..9332
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        9357..9387
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             9441..10097
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             10442..10744
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(10788..10912)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             10920..10937
                     /codon_start=1
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
                     /translation="LVPRGS"
     CDS             10938..10961
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /translation="DYKDDDDK"
     CDS             10968..10985
                     /codon_start=1
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
                     /translation="LVPRGS"
     CDS             10989..11012
                     /codon_start=1
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
                     /translation="WSHPQFEK"
     CDS             11049..11072
                     /codon_start=1
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
                     /translation="WSHPQFEK"
     CDS             11082..11105
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /translation="DYKDDDDK"
     polyA_signal    complement(11110..11244)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 gccccgccgc cggacgaact aaacctgact acggcatctc tgccccttct tcgctggtac
       61 gaggagcgct tttgttttgt attcggggca gtgcatgtaa tcccttcagt tggttggtac
      121 aacttgccaa ctgggccctg ttccacatgt gacacggggg gggaccaaac acaaaggggt
      181 tctctgactg tagttgacat ccttataaat ggatgtgcac atttgccaac actgagtggc
      241 tttcatcctg gagcagactt tgcatgctgt ggactgcaac acaacattgc ctttatgtgt
      301 aactcttggc tgaagctctt acaccaatgc tgggggacat gtacctccca ggggcccagg
      361 aagactacgg gaggctacac caacgtcaat cagaggggcc tgtgtagcta ccgataagcg
      421 gaccctcaag agggcattag caatagtgtt tataaggccc ccttgttaac cctaaacggg
      481 tagcatatgc ttcccgggta gtagtatata ctatccagac taaccctaat tcaatagcat
      541 atgttaccca acgggaagca tatgctatcg aattagggtt agtaaaaggg tcctaaggaa
      601 cagcgatatc tcccacccca tgagctgtca cggttttatt tacatggggt caggattcca
      661 cgagggtagt gaaccatttt agtcacaagg gcagtggctg aagatcaagg agcgggcagt
      721 gaactctcct gaatcttcgc ctgcttcttc attctccttc gtttagctaa tagaataact
      781 gctgagttgt gaacagtaag gtgtatgtga ggtgctcgaa aacaaggttt caggtgacgc
      841 ccccagaata aaatttggac ggggggttca gtggtggcat tgtgctatga caccaatata
      901 accctcacaa accccttggg caataaatac tagtgtagga atgaaacatt ctgaatatct
      961 ttaacaatag aaatccatgg ggtggggaca agccgtaaag actggatgtc catctcacac
     1021 gaatttatgg ctatgggcaa cacataatcc tagtgcaata tgatactggg gttattaaga
     1081 tgtgtcccag gcagggacca agacaggtga accatgttgt tacactctat ttgtaacaag
     1141 gggaaagaga gtggacgccg acagcagcgg actccactgg ttgtctctaa cacccccgaa
     1201 aattaaacgg ggctccacgc caatggggcc cataaacaaa gacaagtggc cactcttttt
     1261 tttgaaattg tggagtgggg gcacgcgtca gcccccacac gccgccctgc ggttttggac
     1321 tgtaaaataa gggtgtaata acttggctga ttgtaacccc gctaaccact gcggtcaaac
     1381 cacttgccca caaaaccact aatggcaccc cggggaatac ctgcataagt aggtgggcgg
     1441 gccaagatag gggcgcgatt gctgcgatct ggaggacaaa ttacacacac ttgcgcctga
     1501 gcgccaagca cagggttgtt ggtcctcata ttcacgaggt cgctgagagc acggtgggct
     1561 aatgttgcca tgggtagcat atactaccca aatatctgga tagcatatgc tatcctaatc
     1621 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     1681 tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc tatcctaatc
     1741 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatc
     1801 tgtatccggg tagcatatgc tatcctaata gagattaggg tagtatatgc tatcctaatt
     1861 tatatctggg tagcatatac tacccaaata tctggatagc atatgctatc ctaatctata
     1921 tctgggtagc atatgctatc ctaatctata tctgggtagc ataggctatc ctaatctata
     1981 tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc ctaatttata
     2041 tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata
     2101 tctgggtagt atatgctatc ctaatctgta tccgggtagc atatgctatc ctcatgcata
     2161 tacagtcagc atatgatacc cagtagtaga gtgggagtgc tatcctttgc atatgccgcc
     2221 acctcccaag ggggcgtgaa ttttcgctgc ttgtcctttt cctgcatgct ggttgctccc
     2281 attcttaggt gaatttaagg aggccaggct aaagccgtcg catgtctgat tgctcaccag
     2341 gtaaatgtcg ctaatgtttt ccaacgcgag aaggtgttga gcgcggagct gagtgacgtg
     2401 acaacatggg tatgcccaat tgccccatgt tgggaggacg aaaatggtga caagacagat
     2461 ggccagaaat acaccaacag cacgcatgat gtctactggg gatttattct ttagtgcggg
     2521 ggaatacacg gcttttaata cgattgaggg cgtctcctaa caagttacat cactcctgcc
     2581 cttcctcacc ctcatctcca tcacctcctt catctccgtc atctccgtca tcaccctccg
     2641 cggcagcccc ttccaccata ggtggaaacc agggaggcaa atctactcca tcgtcaaagc
     2701 tgcacacagt caccctgata ttgcaggtag gagcgggctt tgtcataaca aggtccttaa
     2761 tcgcatcctt caaaacctca gcaaatatat gagtttgtaa aaagaccatg aaataacaga
     2821 caatggactc ccttagcggg ccaggttgtg ggccgggtcc aggggccatt ccaaagggga
     2881 gacgactcaa tggtgtaaga cgacattgtg gaatagcaag ggcagttcct cgccttaggt
     2941 tgtaaaggga ggtcttacta cctccatata cgaacacacc ggcgacccaa gttccttcgt
     3001 cggtagtcct ttctacgtga ctcctagcca ggagagctct taaaccttct gcaatgttct
     3061 caaatttcgg gttggaacct ccttgaccac gatgctttcc aaaccaccct ccttttttgc
     3121 gcctgcctcc atcaccctga ccccggggtc cagtgcttgg gccttctcct gggtcatctg
     3181 cggggccctg ctctatcgct cccgggggca cgtcaggctc accatctggg ccaccttctt
     3241 ggtggtattc aaaataatcg gcttccccta cagggtggaa aaatggcctt ctacctggag
     3301 ggggcctgcg cggtggagac ccggatgatg atgactgact actgggactc ctgggcctct
     3361 tttctccacg tccacgacct ctccccctgg ctctttcacg acttcccccc ctggctcttt
     3421 cacgtcctct accccggcgg cctccactac ctcctcgacc ccggcctcca ctacctcctc
     3481 gaccccggcc tccactgcct cctcgacccc ggcctccacc tcctgctcct gcccctcctg
     3541 ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tgctcctgcc cctcctgccc
     3601 ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc tcctgcccct cctcctgctc
     3661 ctgcccctcc tgcccctcct cctgctcctg cccctcctgc ccctcctgct cctgcccctc
     3721 ctgcccctcc tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct gctcctgccc
     3781 ctcctgctcc tgcccctcct gctcctgccc ctcctgcccc tcctgcccct cctcctgctc
     3841 ctgcccctcc tgctcctgcc cctcctgccc ctcctgcccc tcctgctcct gcccctcctc
     3901 ctgctcctgc ccctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctgcccctc
     3961 ctcctgctcc tgcccctcct cctgctcctg cccctcctgc ccctcctgcc cctcctcctg
     4021 ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tcctgctcct gcccctcctg
     4081 cccctcctgc ccctcctcct gctcctgccc ctcctcctgc tcctgcccct cctgcccctc
     4141 ctgcccctcc tgcccctcct cctgctcctg cccctcctcc tgctcctgcc cctcctgctc
     4201 ctgcccctcc cgctcctgct cctgctcctg ttccaccgtg ggtccctttg cagccaatgc
     4261 aacttggacg tttttggggt ctccggacac catctctatg tcttggccct gatcctgagc
     4321 cgcccggggc tcctggtctt ccgcctcctc gtcctcgtcc tcttccccgt cctcgtccat
     4381 ggttatcacc ccctcttctt tgaggtccac tgccgccgga gccttctggt ccagatgtgt
     4441 ctcccttctc tcctaggcca tttccaggtc ctgtacctgg cccctcgtca gacatgattc
     4501 acactaaaag agatcaatag acatctttat tagacgacgc tcagtgaata cagggagtgc
     4561 agactcctgc cccctccaac agccccccca ccctcatccc cttcatggtc gctgtcagac
     4621 agatccaggt ctgaaaattc cccatcctcc gaaccatcct cgtcctcatc accaattact
     4681 cgcagcccgg aaaactcccg ctgaacatcc tcaagatttg cgtcctgagc ctcaagccag
     4741 gcctcaaatt cctcgtcccc ctttttgctg gacggtaggg atggggattc tcgggacccc
     4801 tcctcttcct cttcaaggtc accagacaga gatgctactg gggcaacgga agaaaagctg
     4861 ggtgcggcct gtgaggatca gcttatcgat gataagctgt caaacatgag aattcttgaa
     4921 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     4981 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     5041 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     5101 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     5161 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     5221 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     5281 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     5341 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
     5401 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     5461 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     5521 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     5581 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     5641 acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg
     5701 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     5761 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     5821 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     5881 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     5941 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     6001 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     6061 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     6121 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     6181 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     6241 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     6301 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     6361 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     6421 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     6481 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     6541 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     6601 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     6661 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     6721 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     6781 gctgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt
     6841 ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa
     6901 tccgttagcg aggccatcca gcctcgcgtc gaactagatg atccgctgtg gaatgtgtgt
     6961 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     7021 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     7081 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     7141 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     7201 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     7261 tttggagggt gacacgccac gaccggtgcc gccaccatcc cctgacccac gcccctgacc
     7321 cctcacaagg agacgacctt ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     7381 cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     7441 gcgccacacc gtcgaccccg accgccacat cgaacgcgtc accgagctgc aagaactctt
     7501 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     7561 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     7621 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     7681 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     7741 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     7801 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     7861 gctcggcttc accgtcaccg ccgacgtcga gtgcccgaag gaccgcgcga cctggtgcat
     7921 gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag
     7981 cgcacgaccc ggtccgacgg cggcccacgg gtcccagggg ggtcgacctc gaaacttgtt
     8041 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     8101 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     8161 ctggatcaga tccgaacccc ttcctcgacc aattctcatg tttgacagct tatcatcgca
     8221 gatccgggca acgttgttgc attgctgcag gcgcagaact ggtaggtatg gaagatctat
     8281 acattgaatc aatattggca attagccata ttagtcattg gttatatagc ataaatcaat
     8341 attggctatt ggccattgca tacgttgtat ctatatcata atatgtacat ttatattggc
     8401 tcatgtccaa tatgaccgcc atgttgacat tgattattga ctagttatta atagtaatca
     8461 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     8521 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     8581 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     8641 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtccgcc ccctattgac
     8701 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt acgggacttt
     8761 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     8821 cagtacacca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     8881 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     8941 aataaccccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     9001 agcagagctc gtttagtgaa ccgtcagatc tctagaagct gggtacctta aggcgccagc
     9061 tgatcaagct tctgcctgcc gcctgcctgc ctgccactga gggttcccag caccatgagg
     9121 gcctggatct tctttctcct ttgcctggcc gggagggctc tggcagcccc gctagcactg
     9181 gttccgcgtg gatccggtgg tggtatcaca agtttgtaca aaaaagctga acgagaaacg
     9241 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
     9301 tactgtaaaa cacaacatat ccagtcacta tggcggccgc attaggcacc ccaggcttta
     9361 cactttatgc ttccggctcg tataatgtgt ggattttgag ttaggatccg ttgagatttt
     9421 caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat
     9481 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata
     9541 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca
     9601 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc
     9661 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg
     9721 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc
     9781 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt
     9841 tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca
     9901 ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg
     9961 gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg
    10021 ccgtttgtga tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg
    10081 agtggcaggg cggggcgtaa acgcgtggat ccggcttact aaaagccaga taacagtatg
    10141 cgtatttgcg cgctgatttt tgcggtataa gaatatatac tgatatgtat acccgaagta
    10201 tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta
    10261 tcagttgctc aaggcatata tgatgtcaat atctccggtc tggtaagcac aaccatgcag
    10321 aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct
    10381 gaggtcgccc ggtttattga aatgaacggc tcttttgctg acgagaacag gggctggtga
    10441 aatgcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt
    10501 acagagtgat attattgaca cgcccgggcg acggatggtg atccccctgg ccagtgcacg
    10561 tctgctgtca gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag
    10621 ctggcgcatg atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt
    10681 ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg
    10741 aatataaatg tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga
    10801 tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat
    10861 tgatatttat atcattttac gtttctcgtt cagctttctt gtacaaagtg gtgaaagctc
    10921 tggttccgcg tggatccgac tacaaggacg acgatgacaa gagatccctg gttcctcgcg
    10981 gctctgcttg gagccaccct cagttcgaga aaggtggagg ttccggaggt ggatctggag
    11041 gtggttcatg gagccaccca cagttcgaaa aataaagatc cgactacaag gacgacgatg
    11101 acaagtaatg atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
    11161 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
    11221 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
    11281 cagggggagg tggggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
    11341 attatgatcc ggctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
    11401 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
    11461 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgaccggtc gactctaga
//
