LOCUS       40924_14410        5374 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Diatom vector pDEST-N-HA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5374)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5374)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5374)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5374
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(6..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      865..1100
                     /label=FcpB promoter
                     /note="FcpB promoter"
                     /regulatory_class="promoter"
     CDS             1114..1140
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             1141..1167
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             1183..1209
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     protein_bind    1225..1349
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1374..1404
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1458..2114
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             2459..2761
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(2805..2929)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      3097..3170
                     /label=FcpA terminator
                     /note="FcpA terminator"
                     /regulatory_class="terminator"
     promoter        complement(3189..3207)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3228..3244)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3252..3268)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3276..3306)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3321..3342)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3630..4218)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4392..5249)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5250..5354)
                     /label=AmpR promoter
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggaatc tcgcccattc atggtgtata caagttcaac atccaaagct ggaacttttg
      721 gaaacagaaa gaatgtccga atagggcacg gcgtgccgta ttgttggagt ggactagcag
      781 aaagtgagga aggcacagga tgagtttcct cgagacacat agcttcagcg tcgtgtaggc
      841 taggcagagg tgagttttct cgagacatac cttcagcgtc gtcttcactg tcacagtcaa
      901 ctgacagtaa tcgttgatcc ggagagattc aaaattcaat ctgtttggac ctggataaga
      961 cacaagagcg acatcctgac atgaacgccg taaacagcaa atcctggttg aacacgtatc
     1021 cttttggggg cctccagcta cgacgctcgc cccagctggg gcttccttac tatacacagc
     1081 gcatatttca cggttgccag cggccgcatg cgatacccct acgacgtgcc cgactacgcc
     1141 tacccctacg acgtgcccga ctacgccgat cgatccggac cgtaccccta cgacgtgccc
     1201 gactacgccg ctggatcccc catcacaagt ttgtacaaaa aagctgaacg agaaacgtaa
     1261 aatgatataa atatcaatat attaaattag attttgcata aaaaacagac tacataatac
     1321 tgtaaaacac aacatatcca gtcactatgg cggccgcatt aggcacccca ggctttacac
     1381 tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg agattttcag
     1441 gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc
     1501 aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc
     1561 agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt
     1621 tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta
     1681 tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt
     1741 tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc
     1801 agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc
     1861 ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca
     1921 gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca
     1981 aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg
     2041 tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt
     2101 ggcagggcgg ggcgtaaacg cgtggatccg gcttactaaa agccagataa cagtatgcgt
     2161 atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc cgaagtatgt
     2221 caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg acagctatca
     2281 gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac catgcagaat
     2341 gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg gatggctgag
     2401 gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg ctggtgaaat
     2461 gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg tggatgtaca
     2521 gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca gtgcacgtct
     2581 gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg atgaaagctg
     2641 gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg aagaagtggc
     2701 tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat
     2761 ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt gactggatat
     2821 gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga
     2881 tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtg atgggctgca
     2941 ggaattccga gctacctcga ctttggctgg gacactttca gtgaggacaa gaagcttcag
     3001 aagcgtgcta tcgaactcaa ccagggacgt gcggcacaaa tgggcatcct tgctctcatg
     3061 gtgcacgaac agttgggagt ctctatcctt ccttaaaaat ttaattttca ttagttgcag
     3121 tcactccgct ttggtttcac agtcaggaat aacactagct cgtcttcacc ggtacccagc
     3181 ttttgttccc tttagtgagg gttaatttcg agcttggcgt aatcatggtc atagctgttt
     3241 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     3301 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     3361 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     3421 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     3481 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     3541 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     3601 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     3661 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     3721 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     3781 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     3841 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     3901 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     3961 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     4021 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     4081 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     4141 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     4201 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     4261 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     4321 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     4381 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     4441 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     4501 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     4561 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     4621 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     4681 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     4741 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     4801 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     4861 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     4921 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     4981 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     5041 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     5101 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     5161 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     5221 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     5281 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     5341 ataggggttc cgcgcacatt tccccgaaaa gtgc
//
