LOCUS       40924_14405        5984 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Diatom vector pDEST-N-EYFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5984)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5984)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5984)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5984
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(6..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      865..1100
                     /label=FcpB promoter
                     /note="FcpB promoter"
                     /regulatory_class="promoter"
     CDS             1108..1824
                     /label=EYFP
                     /note="enhanced YFP"
     protein_bind    1837..1961
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1986..2016
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2070..2726
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             3071..3373
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(3417..3541)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      3709..3780
                     /label=FcpA terminator
                     /note="FcpA terminator"
                     /regulatory_class="terminator"
     promoter        complement(3799..3817)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3838..3854)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3862..3878)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3886..3916)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3931..3952)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4240..4828)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5002..5859)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5860..5964)
                     /label=AmpR promoter
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggaatc tcgcccattc atggtgtata caagttcaac atccaaagct ggaacttttg
      721 gaaacagaaa gaatgtccga atagggcacg gcgtgccgta ttgttggagt ggactagcag
      781 aaagtgagga aggcacagga tgagtttcct cgagacacat agcttcagcg tcgtgtaggc
      841 taggcagagg tgagttttct cgagacatac cttcagcgtc gtcttcactg tcacagtcaa
      901 ctgacagtaa tcgttgatcc ggagagattc aaaattcaat ctgtttggac ctggataaga
      961 cacaagagcg acatcctgac atgaacgccg taaacagcaa atcctggttg aacacgtatc
     1021 cttttggggg cctccagcta cgacgctcgc cccagctggg gcttccttac tatacacagc
     1081 gcatatttca cggttgccag cggccgcatg gtgagcaagg gcgaggagct gttcaccggg
     1141 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     1201 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     1261 ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgcagtgc
     1321 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     1381 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     1441 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     1501 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     1561 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     1621 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     1681 ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac
     1741 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     1801 ctcggcatgg acgagctgta caagggatcc cccatcacaa gtttgtacaa aaaagctgaa
     1861 cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag
     1921 actacataat actgtaaaac acaacatatc cagtcactat ggcggccgca ttaggcaccc
     1981 caggctttac actttatgct tccggctcgt ataatgtgtg gattttgagt taggatccgt
     2041 cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg
     2101 ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat
     2161 gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa
     2221 ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc
     2281 cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt
     2341 gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg
     2401 acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc
     2461 tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg
     2521 tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt
     2581 tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg
     2641 ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt
     2701 actgcgatga gtggcagggc ggggcgtaaa cgcgtggatc cggcttacta aaagccagat
     2761 aacagtatgc gtatttgcgc gctgattttt gcggtataag aatatatact gatatgtata
     2821 cccgaagtat gtcaaaaaga ggtatgctat gaagcagcgt attacagtga cagttgacag
     2881 cgacagctat cagttgctca aggcatatat gatgtcaata tctccggtct ggtaagcaca
     2941 accatgcaga atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa
     3001 gggatggctg aggtcgcccg gtttattgaa atgaacggct cttttgctga cgagaacagg
     3061 ggctggtgaa atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt
     3121 tgtggatgta cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc
     3181 cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg
     3241 ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg
     3301 ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat
     3361 gttctgggga atataaatgt caggctccct tatacacagc cagtctgcag gtcgaccata
     3421 gtgactggat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat
     3481 ttaatatatt gatatttata tcattttacg tttctcgttc agctttcttg tacaaagtgg
     3541 tgatgggctg caggaattcc gagctacctc gactttggct gggacacttt cagtgaggac
     3601 aagaagcttc agaagcgtgc tatcgaactc aaccagggac gtgcggcaca aatgggcatc
     3661 cttgctctca tggtgcacga acagttggga gtctctatcc ttccttaaaa atttaatttt
     3721 cattagttgc agtcactccg ctttggtttc acagtcagga ataacactag ctcgtcttca
     3781 ggtacccagc ttttgttccc tttagtgagg gttaatttcg agcttggcgt aatcatggtc
     3841 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     3901 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     3961 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     4021 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     4081 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     4141 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     4201 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     4261 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     4321 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     4381 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     4441 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     4501 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     4561 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     4621 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     4681 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     4741 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     4801 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     4861 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     4921 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     4981 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     5041 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     5101 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     5161 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     5221 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     5281 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     5341 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     5401 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     5461 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     5521 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     5581 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     5641 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     5701 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     5761 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     5821 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     5881 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     5941 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgc
//
