LOCUS       40924_14380        6038 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian vector pDEST-Etag, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6038)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6038)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6038)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6038)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      complement(1..17)
                     /label=T7 promoter
                     /note="T7 promoter"
                     /regulatory_class="promoter"
     polyA_signal    complement(22..156)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(269..287)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(308..324)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(332..348)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(356..386)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(401..422)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(710..1298)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1472..2329)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2330..2434)
                     /label=AmpR promoter
     rep_origin      complement(2460..2915)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2989..3973
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus
                     major immediate early transcription unit IE94"
     promoter        4008..4026
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    4073..4111
                     /label=E-tag
                     /note="E-tag"
     protein_bind    4142..4266
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        4440..4542
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             4543..5199
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             5544..5846
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(5890..6014)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tatagtgagt cgtattacgt agatccagac atgataagat acattgatga gtttggacaa
       61 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
      121 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
      181 atgtttcagg ttcaggggga ggtgtgggag gttttttaat tcgcggccgc ggcgccaatg
      241 cattgggccc ggtacccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt
      301 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
      361 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
      421 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
      481 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
      541 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
      601 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
      661 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
      721 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
      781 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
      841 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
      901 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
      961 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     1021 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     1081 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     1141 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     1201 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     1261 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     1321 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     1381 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     1441 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     1501 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     1561 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     1621 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     1681 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     1741 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     1801 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     1861 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     1921 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     1981 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     2041 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     2101 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     2161 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     2221 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     2281 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     2341 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     2401 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta
     2461 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     2521 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     2581 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     2641 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     2701 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     2761 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     2821 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     2881 acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa
     2941 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagtcga ccatagccaa
     3001 ttcaatatgg cgtatatgga ctcatgccaa ttcaatatgg tggatctgga cctgtgccaa
     3061 ttcaatatgg cgtatatgga ctcgtgccaa ttcaatatgg tggatctgga ccccagccaa
     3121 ttcaatatgg cggacttggc accatgccaa ttcaatatgg cggacttggc actgtgccaa
     3181 ctggggaggg gtctacttgg cacggtgcca agtttgagga ggggtcttgg ccctgtgcca
     3241 agtccgccat attgaattgg catggtgcca ataatggcgg ccatattggc tatatgccag
     3301 gatcaatata taggcaatat ccaatatggc cctatgccaa tatggctatt ggccaggttc
     3361 aatactatgt attggcccta tgccatatag tattccatat atgggttttc ctattgacgt
     3421 agatagcccc tcccaatggg cggtcccata taccatatat ggggcttcct aataccgccc
     3481 atagccactc ccccattgac gtcaatggtc tctatatatg gtctttccta ttgacgtcat
     3541 atgggcggtc ctattgacgt atatggcgcc tcccccattg acgtcaatta cggtaaatgg
     3601 cccgcctggc tcaatgccca ttgacgtcaa taggaccacc caccattgac gtcaatggga
     3661 tggctcattg cccattcata tccgttctca cgccccctat tgacgtcaat gacggtaaat
     3721 ggcccacttg gcagtacatc aatatctatt aatagtaact tggcaagtac attactattg
     3781 gaaggacgcc agggtacatt ggcagtactc ccattgacgt caatggcggt aaatggcccg
     3841 cgatggctgc caagtacatc cccattgacg tcaatgggga ggggcaatga cgcaaatggg
     3901 cgttccattg acgtaaatgg gcggtaggcg tgcctaatgg gaggtctata taagcaatgc
     3961 tcgtttaggg aaccgccatt ctgcctgggg acgtcggagc aagcttgatt taggtgacac
     4021 tatagaatac aagctacttg ttctttttgc aggatcccat cgatccccca tgggtgcgcc
     4081 ggtgccgtat ccggacccgc tggaaccgcg tgcggcccat cgattcgaat tcaaggatca
     4141 aacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt
     4201 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc
     4261 actatggcgg ccgcgggtga tgctgccaac ttagcggccg ctaagttggc agcatcaccc
     4321 gacgcacttt gcgccgaata aatacctgtg acggaagatc acttcgcaga ataaataaat
     4381 cctggtgtcc ctgttgatac cgggaagccc tgggccaact tttggcgaaa atgagacgtt
     4441 gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact accgggcgta
     4501 ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
     4561 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
     4621 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
     4681 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
     4741 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
     4801 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
     4861 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
     4921 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
     4981 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
     5041 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
     5101 ctggcgattc aggttcatca tgccgtctgt gatggcttcc atgtcggcag aatgcttaat
     5161 gaattacaac agtactgcga tgagtggcag ggcggggcgt aatctagagg atccggctta
     5221 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
     5281 actgatatgt atacccgaag tatgtcaaaa agaggtgtgc tatgaagcag cgtattacag
     5341 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg
     5401 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc
     5461 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc
     5521 tgacgagaac agggactggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
     5581 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg
     5641 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg
     5701 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg
     5761 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
     5821 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
     5881 caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     5941 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc
     6001 ttgtacaaag tggttcgatc ctctcgagcc tctagaac
//
