LOCUS       40924_14370        5391 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Diatom vector pDEST-C-HA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5391)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5391)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5391)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(6..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      865..1100
                     /label=FcpB promoter
                     /note="FcpB promoter"
                     /regulatory_class="promoter"
     primer_bind     1108..1124
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1141..1265
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1290..1320
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1374..2030
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             2375..2677
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(2721..2845)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2857..2883
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             2884..2910
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             2926..2952
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     regulatory      3114..3187
                     /label=FcpA terminator
                     /note="FcpA terminator"
                     /regulatory_class="terminator"
     promoter        complement(3206..3224)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3245..3261)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3269..3285)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3293..3323)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3338..3359)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3647..4235)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4409..5266)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5267..5371)
                     /label=AmpR promoter
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
      301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
      361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggaatc tcgcccattc atggtgtata caagttcaac atccaaagct ggaacttttg
      721 gaaacagaaa gaatgtccga atagggcacg gcgtgccgta ttgttggagt ggactagcag
      781 aaagtgagga aggcacagga tgagtttcct cgagacacat agcttcagcg tcgtgtaggc
      841 taggcagagg tgagttttct cgagacatac cttcagcgtc gtcttcactg tcacagtcaa
      901 ctgacagtaa tcgttgatcc ggagagattc aaaattcaat ctgtttggac ctggataaga
      961 cacaagagcg acatcctgac atgaacgccg taaacagcaa atcctggttg aacacgtatc
     1021 cttttggggg cctccagcta cgacgctcgc cccagctggg gcttccttac tatacacagc
     1081 gcatatttca cggttgccag cggccgctct agaactagtg gatcccccgg gggagatatc
     1141 acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta
     1201 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca
     1261 ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg
     1321 tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga
     1381 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
     1441 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
     1501 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
     1561 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg
     1621 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
     1681 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
     1741 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
     1801 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
     1861 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
     1921 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
     1981 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taaacgcgtg
     2041 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
     2101 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca
     2161 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
     2221 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
     2281 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
     2341 ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa
     2401 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
     2461 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga
     2521 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
     2581 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
     2641 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
     2701 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag
     2761 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
     2821 gttcagcttt cttgtacaaa gtggtgatat ccccgatacc cctacgacgt gcccgactac
     2881 gcctacccct acgacgtgcc cgactacgcc gatcgatccg gaccgtaccc ctacgacgtg
     2941 cccgactacg ccgcttaaga attccgagct acctcgactt tggctgggac actttcagtg
     3001 aggacaagaa gcttcagaag cgtgctatcg aactcaacca gggacgtgcg gcacaaatgg
     3061 gcatccttgc tctcatggtg cacgaacagt tgggagtctc tatccttcct taaaaattta
     3121 attttcatta gttgcagtca ctccgctttg gtttcacagt caggaataac actagctcgt
     3181 cttcaccggt acccagcttt tgttcccttt agtgagggtt aatttcgagc ttggcgtaat
     3241 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     3301 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     3361 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     3421 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     3481 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     3541 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     3601 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     3661 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     3721 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     3781 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     3841 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     3901 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     3961 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     4021 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     4081 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     4141 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     4201 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     4261 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     4321 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     4381 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     4441 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     4501 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     4561 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     4621 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     4681 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     4741 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     4801 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     4861 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     4921 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     4981 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     5041 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     5101 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     5161 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     5221 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     5281 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     5341 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg c
//
