LOCUS       40924_14360        6526 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Gateway(R) destination vector for expressing proteins in insect 
            cells using the Bac-to-Bac(R) baculovirus system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6526)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6526)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6526
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..115
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     protein_bind    160..284
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        431..533
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             534..1190
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             1535..1837
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1881..2005)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    2137..2271
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  complement(2300..2465)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2648..3103
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3130..3234
                     /label=AmpR promoter
     CDS             3235..4092
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4266..4854
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  complement(5157..5381)
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(5451..5981)
                     /codon_start=1
                     /label=GmR
                     /note="gentamycin acetyltransferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(6170..6198)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 cgtatactcc ggaatattaa tagatcatgg agataattaa aatgataacc atctcgcaaa
       61 taaataagta ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta taaatattcc
      121 ggattattca taccgtccca ccatcgggcg cggatcatca caagtttgta caaaaaagct
      181 gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa
      241 cagactacat aatactgtaa aacacaacat atccagtcac tatggcggcc gctaagttgg
      301 cagcatcacc cgacgcactt tgcgccgaat aaatacctgt gacggaagat cacttcgcag
      361 aataaataaa tcctggtgtc cctgttgata ccgggaagcc ctgggccaac ttttggcgaa
      421 aatgagacgt tgatcggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac
      481 taccgggcgt attttttgag ttatcgagat tttcaggagc taaggaagct aaaatggaga
      541 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
      601 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
      661 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
      721 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg
      781 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
      841 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
      901 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
      961 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
     1021 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
     1081 tgctgatgcc gctggcgatt caggttcatc atgccgtctg tgatggcttc catgtcggca
     1141 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taaacgcgtg
     1201 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
     1261 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtgtg ctatgaagca
     1321 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
     1381 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
     1441 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
     1501 ggctcttttg ctgacgagaa cagggactgg tgaaatgcag tttaaggttt acacctataa
     1561 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
     1621 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga
     1681 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
     1741 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
     1801 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
     1861 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag
     1921 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
     1981 gttcagcttt cttgtacaaa gtggtgatag cttgtcgaga agtactagag gatcataatc
     2041 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     2101 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     2161 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     2221 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat ctgatcactg
     2281 cttgagccta ggagatccga accagataag tgaaatctag ttccaaacta ttttgtcatt
     2341 tttaattttc gtattagctt acgacgctac acccagttcc catctatttt gtcactcttc
     2401 cctaaataat ccttaaaaac tccatttcca cccctcccag ttcccaacta ttttgtccgc
     2461 ccacagcggg gcatttttct tcctgttatg tttttaatca aacatcctgc caactccatg
     2521 tgacaaaccg tcatcttcgg ctactttttc tctgtcacag aatgaaaatt tttctgtcat
     2581 ctcttcgtta ttaatgtttg taattgactg aatatcaacg cttatttgca gcctgaatgg
     2641 cgaatggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     2701 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     2761 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     2821 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     2881 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     2941 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     3001 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     3061 aaatttaacg cgaattttaa caaaatatta acgtttacaa tttcaggtgg cacttttcgg
     3121 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     3181 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     3241 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     3301 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     3361 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     3421 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     3481 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     3541 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     3601 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     3661 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     3721 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     3781 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     3841 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     3901 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     3961 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     4021 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     4081 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     4141 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     4201 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     4261 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     4321 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     4381 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     4441 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     4501 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     4561 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     4621 acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc
     4681 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     4741 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     4801 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     4861 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     4921 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     4981 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     5041 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcag accagccgcg
     5101 taacctggca aaatcggtta cggttgagta ataaatggat gccctgcgta agcgggtgtg
     5161 ggcggacaat aaagtcttaa actgaacaaa atagatctaa actatgacaa taaagtctta
     5221 aactagacag aatagttgta aactgaaatc agtccagtta tgctgtgaaa aagcatactg
     5281 gacttttgtt atggctaaag caaactcttc attttctgaa gtgcaaattg cccgtcgtat
     5341 taaagagggg cgtggccaag ggcatggtaa agactatatt cgcggcgttg tgacaattta
     5401 ccgaacaact ccgcggccgg gaagccgatc tcggcttgaa cgaattgtta ggtggcggta
     5461 cttgggtcga tatcaaagtg catcacttct tcccgtatgc ccaactttgt atagagagcc
     5521 actgcgggat cgtcaccgta atctgcttgc acgtagatca cataagcacc aagcgcgttg
     5581 gcctcatgct tgaggagatt gatgagcgcg gtggcaatgc cctgcctccg gtgctcgccg
     5641 gagactgcga gatcatagat atagatctca ctacgcggct gctcaaacct gggcagaacg
     5701 taagccgcga gagcgccaac aaccgcttct tggtcgaagg cagcaagcgc gatgaatgtc
     5761 ttactacgga gcaagttccc gaggtaatcg gagtccggct gatgttggga gtaggtggct
     5821 acgtctccga actcacgacc gaaaagatca agagcagccc gcatggattt gacttggtca
     5881 gggccgagcc tacatgtgcg aatgatgccc atacttgagc cacctaactt tgttttaggg
     5941 cgactgccct gctgcgtaac atcgttgctg ctgcgtaaca tcgttgctgc tccataacat
     6001 caaacatcga cccacggcgt aacgcgcttg ctgcttggat gcccgaggca tagactgtac
     6061 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
     6121 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gtttacgaac
     6181 cgaacaggct tatgtcaact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc
     6241 ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc tggctggcga acgagcgcaa
     6301 ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg ctgttcttct acggcaaggt
     6361 gctgtgcacg gatctgccct ggcttcagga gatcggaaga cctcggccgt cgcggcgctt
     6421 gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc ggttttctgg aaggcgagca
     6481 tcgtttgttc gcccaggact ctagctatag ttctagtggt tggcta
//
