LOCUS       pDEST17.        6354 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Gateway(R) destination vector for inducible high-level expression of
            N-terminally 6xHis-tagged proteins in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    pDEST17
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6354)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6354)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6354
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..39
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             71..93
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             101..103
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             113..130
                     /label=6xHis
                     /note="6xHis affinity tag"
     protein_bind    140..264
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        289..319
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             373..1029
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             1374..1676
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(1720..1844)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      1911..1958
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(2324..2352)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     promoter        2465..2569
                     /label=AmpR promoter
     CDS             2570..3427
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3601..4189
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(4375..4517)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(4622..4810)
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
ORIGIN
        1 agatctcgat cccgcgaaat taatacgact cactataggg agaccacaac ggtttccctc
       61 tagaaataat tttgtttaac tttaagaagg agatatacat atgtcgtact accatcacca
      121 tcaccatcac ctcgaatcaa caagtttgta caaaaaagct gaacgagaaa cgtaaaatga
      181 tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa
      241 aacacaacat atccagtcac tatggcggcc gcattaggca ccccaggctt tacactttat
      301 gcttccggct cgtataatgt gtggattttg agttaggatc cgtcgagatt ttcaggagct
      361 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
      421 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
      481 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
      541 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca
      601 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
      661 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
      721 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
      781 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
      841 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
      901 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtctgt
      961 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
     1021 ggcggggcgt aaagatctgg atccggctta ctaaaagcca gataacagta tgcgtatttg
     1081 cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa
     1141 agaggtgtgc tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc
     1201 tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc
     1261 ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc
     1321 ccggtttatt gaaatgaacg gctcttttgc tgacgagaac agggactggt gaaatgcagt
     1381 ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg
     1441 atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt
     1501 cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca
     1561 tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc
     1621 tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa
     1681 tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg gatatgttgt
     1741 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt
     1801 atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggttgattc gaggctgcta
     1861 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
     1921 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg
     1981 gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc
     2041 gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg
     2101 catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg
     2161 tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct
     2221 acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac
     2281 ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg
     2341 ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt
     2401 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     2461 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     2521 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     2581 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     2641 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     2701 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     2761 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc
     2821 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     2881 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     2941 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     3001 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     3061 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat
     3121 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     3181 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     3241 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     3301 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     3361 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     3421 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     3481 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     3541 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3601 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3661 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3721 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     3781 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3841 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     3901 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     3961 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     4021 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     4081 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     4141 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     4201 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     4261 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     4321 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
     4381 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc
     4441 agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg
     4501 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     4561 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     4621 agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt
     4681 ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct
     4741 ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct
     4801 gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga
     4861 tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg
     4921 aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc
     4981 agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc
     5041 atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac
     5101 tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc
     5161 agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc
     5221 aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc
     5281 aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg
     5341 atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc
     5401 caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt
     5461 ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc
     5521 tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat
     5581 cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc
     5641 ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc
     5701 gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag
     5761 caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa
     5821 acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac
     5881 cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac
     5941 ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc
     6001 ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa
     6061 gggcatcggt cgatcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta
     6121 gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc
     6181 ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga
     6241 gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa
     6301 ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcg
//
