LOCUS       pDEST10.        6708 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Gateway(R) destination vector for expressing N-terminally 
            6xHis-tagged proteins in insect cells using the Bac-to-Bac(R) 
            baculovirus system.
ACCESSION   .
VERSION     .
KEYWORDS    pDEST10
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6708)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6708)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        116..207
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             262..264
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             274..291
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             313..333
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     protein_bind    337..461
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        608..710
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             711..1367
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             1712..2014
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(2058..2182)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    2410..2544
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  complement(2573..2738)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2922..3377
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3404..3508
                     /label=AmpR promoter
     CDS             3509..4366
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4540..5128
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  complement(5431..5655)
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(5725..6255)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(6444..6472)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ccccggatga agtggttcgc atcctcggtt ttctggaagg cgagcatcgt ttgttcgccc
       61 aggactctag ctatagttct agtggttggc tacgtatact ccggaatatt aatagatcat
      121 ggagataatt aaaatgataa ccatctcgca aataaataag tattttactg ttttcgtaac
      181 agttttgtaa taaaaaaacc tataaatatt ccggattatt cataccgtcc caccatcggg
      241 cgcggatctc ggtccgaaac catgtcgtac taccatcacc atcaccatca cgattacgat
      301 atcccaacga ccgaaaacct gtattttcag ggcatcacaa gtttgtacaa aaaagctgaa
      361 cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag
      421 actacataat actgtaaaac acaacatatc cagtcactat ggcggccgct aagttggcag
      481 catcacccga cgcactttgc gccgaataaa tacctgtgac ggaagatcac ttcgcagaat
      541 aaataaatcc tggtgtccct gttgataccg ggaagccctg ggccaacttt tggcgaaaat
      601 gagacgttga tcggcacgta agaggttcca actttcacca taatgaaata agatcactac
      661 cgggcgtatt ttttgagtta tcgagatttt caggagctaa ggaagctaaa atggagaaaa
      721 aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg
      781 catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct
      841 ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg
      901 cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga
      961 tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat
     1021 cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg
     1081 tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt
     1141 tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg
     1201 acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc
     1261 tgatgccgct ggcgattcag gttcatcatg ccgtctgtga tggcttccat gtcggcagaa
     1321 tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa acgcgtggat
     1381 ccggcttact aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa
     1441 gaatatatac tgatatgtat acccgaagta tgtcaaaaag aggtgtgcta tgaagcagcg
     1501 tattacagtg acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat
     1561 atctccggtc tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc
     1621 tggaaagcgg aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc
     1681 tcttttgctg acgagaacag ggactggtga aatgcagttt aaggtttaca cctataaaag
     1741 agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcccgggcg
     1801 acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact
     1861 ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag
     1921 tgtgccggtc tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat
     1981 caaaaacgcc attaacctga tgttctgggg aatataaatg tcaggctccc ttatacacag
     2041 ccagtctgca ggtcgaccat agtgactgga tatgttgtgt tttacagtat tatgtagtct
     2101 gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt
     2161 cagctttctt gtacaaagtg gtgatgccat ggatccggaa ttcaaaggcc tacgtcgacg
     2221 agctcaacta gtgcggccgc tttcgaatct agagcctgca gtctcgaggc atgcggtacc
     2281 aagcttgtcg agaagtacta gaggatcata atcagccata ccacatttgt agaggtttta
     2341 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     2401 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     2461 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     2521 aatgtatctt atcatgtctg gatctgatca ctgcttgagc ctaggagatc cgaaccagat
     2581 aagtgaaatc tagttccaaa ctattttgtc atttttaatt ttcgtattag cttacgacgc
     2641 tacacccagt tcccatctat tttgtcactc ttccctaaat aatccttaaa aactccattt
     2701 ccacccctcc cagttcccaa ctattttgtc cgcccacagc ggggcatttt tcttcctgtt
     2761 atgtttttaa tcaaacatcc tgccaactcc atgtgacaaa ccgtcatctt cggctacttt
     2821 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
     2881 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     2941 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     3001 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     3061 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     3121 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     3181 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     3241 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     3301 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     3361 attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     3421 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     3481 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     3541 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     3601 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     3661 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     3721 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     3781 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     3841 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     3901 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     3961 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     4021 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     4081 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     4141 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     4201 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     4261 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     4321 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     4381 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     4441 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     4501 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     4561 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     4621 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     4681 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     4741 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     4801 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     4861 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     4921 acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     4981 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     5041 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     5101 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     5161 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     5221 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     5281 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     5341 tctgtgcggt atttcacacc gcagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     5401 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     5461 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     5521 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     5581 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     5641 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     5701 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     5761 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     5821 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag
     5881 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     5941 ctcactacgc ggctgctcaa acctgggcag aacgtaagcc gcgagagcgc caacaaccgc
     6001 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     6061 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     6121 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     6181 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     6241 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     6301 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     6361 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     6421 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     6481 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     6541 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     6601 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg ccctggcttc
     6661 aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt ggtgctga
//
