LOCUS       40924_14285        3040 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector encoding the 12 kDa destabilization domain (DD), a mutant of 
            FKBP12.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3040
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..324
                     /codon_start=1
                     /label=DD
                     /note="destabilization domain that can be stabilized by
                     Shield1 in the ProteoTuner(TM) system"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKPE"
     primer_bind     complement(389..405)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(413..429)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(437..467)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(482..503)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(791..1379)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1553..2410)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2411..2515)
                     /label=AmpR promoter
     primer_bind     2989..3005
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 atgggagtgc aggtggaaac catctcccca ggagacgggc gcaccttccc caagcgcggc
       61 cagacctgtg tggtgcacta caccgggatg cttgaagatg gaaagaaagt cgattcctcc
      121 cgggacagaa acaagccctt taagtttatg ctaggcaagc aggaggtgat ccgaggctgg
      181 gaagaagggg ttgcccagat gagtgtgggt cagagagcca aactgactat atctccagat
      241 tatgcctatg gtgccactgg gcacccaggc atcatcccac cacatgccac tctcgtcttc
      301 gatgtggagc ttctaaaacc ggaattgata ccactgcttg ggatcctcta gagtcgatct
      361 gcaggcatgc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
      421 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
      481 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
      541 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
      601 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
      661 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
      721 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
      781 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
      841 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
      901 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
      961 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     1021 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1081 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     1141 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     1201 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     1261 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     1321 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     1381 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     1441 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     1501 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     1561 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     1621 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     1681 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     1741 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     1801 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     1861 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     1921 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     1981 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     2041 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     2101 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     2161 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     2221 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     2281 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     2341 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     2401 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     2461 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     2521 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     2581 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
     2641 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     2701 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
     2761 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     2821 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg
     2881 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     2941 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     3001 ccagtgaatt cgagctcggt acccgcgggg tacgatgaga
//
