LOCUS       V016221                 8715 bp    DNA     circular SYN 09-MAR-2020
DEFINITION  Exported.
ACCESSION   V016221
VERSION     V016221
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8715)
  AUTHORS   Nemeth T, Papp C, Szenzenstein J, Gacser A.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-NOV-2019) Department of Microbiology, University of
            Szeged, Kozep fasor 52, Szeged, Csongrad 6726, Hungary
REFERENCE   2  (bases 1 to 8715)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. v11
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (27-NOV-2019) Department of Microbiology, University of Szeged,
            Kozep fasor 52, Szeged, Csongrad 6726, Hungary"
FEATURES             Location/Qualifiers
     source          1..8715
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /label="AmpR promoter"
     CDS             129..986
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1160..1748
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    2036..2057
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2072..2102
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2134..2150
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        2171..2189
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      2222..3071
                     /label="Candida albicans TDH3 promoter"
                     /note="Candida albicans TDH3 promoter"
                     /regulatory_class="promoter"
     protein_bind    3078..3202
                     /label="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        3227..3257
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             3311..3967
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     CDS             4312..4614
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(4658..4782)
                     /label="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     gene            5064..5840
                     /gene="CPAR2_110290"
                     /label="CPAR2_110290"
     CDS             5064..5840
                     /codon_start=1
                     /transl_table=11
                     /gene="CPAR2_110290"
                     /product="RPS1-like protein"
                     /label="CPAR2_110290"
                     /note="similar to Candida albicans RPS1; recombination
                     target"
                     /protein_id="QID92265.1"
                     /translation="MAVGKNKRLSKGKKGLKKKVVDPFTRKDWFDIKAPTTFENRNVGK
                     TLINRSTGLKNAADGLKGRVFEVCLADLQGSEDHSYRKIKLRVDEVQGKNLLTNFHGLD
                     FTSDKIRSRPLVRKWQSLVEANVTVKTADDYVLRVFAIAFTKRQPNQIKKTTYAQSSKL
                     REIRKKMTEIMQREVSNCTLAQLTSKLIPEVIGREIEKSTQTIFPLQNVHIRKVKLLKQ
                     PKFDLGSLLALHGEGSTEEKGKKVSGGFKDVVLESV"
     misc_feature    5400..5405
                     /gene="CPAR2_110290"
                     /label="StuI site for plasmid linearization"
                     /note="StuI site for plasmid linearization"
     CDS             complement(6068..7186)
                     /gene="LEU2"
                     /label="3-isopropylmalate dehydrogenase"
                     /note="3-isopropylmalate dehydrogenase from Candida
                     maltosa. Accession#: P07139"
     regulatory      complement(7187..8061)
                     /label="Candida maltosa LEU2 promoter"
                     /note="Candida maltosa LEU2 promoter"
                     /regulatory_class="promoter"
     promoter        complement(8076..8094)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8101..8117)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      complement(8258..8713)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cgtgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagctcga aattaaccct cactaaaggg aacaaaagct ggtaccgggc cccccctcga
     2221 gcacacacat acagacaatt gcgatatttt attttagggt caatttggaa cgtcaccata
     2281 cagtattcag tatgattaat ttgacttgca aaatacaaat cgcgtggttc aattccgaaa
     2341 aattgatagt gttccatgaa aaattacaag gagagccgtg aattataaca aatttactca
     2401 cattttggct ggcaatttct ttaaccccct taattttttt catgcagttt attattttac
     2461 gtagaagctt attctctaaa cactcggtta tccgaatata ttttgctttc agagcaattg
     2521 actgatataa taccatcaat tgaataaatc agtatcatca aatgctagtt tgagttcaat
     2581 aaaaggtata atcggtgtta actgaaaggt attgtctaat taattaggtt gcggtttttg
     2641 gatcagctgt ttcaaatcca ggctcgtggt gcacattttt tatttttgtt gtgatgattt
     2701 tttcttttct ttgcttaata gttagttagt aaaatggata atgacgcaaa attgcttctg
     2761 aatggatgat aatgccccgg gtatggcgtg tcccgtacaa atcatattat gcaaaaatag
     2821 ccaaaataga caaaaatagg tggaaattgt ttcaaccaac ccactcaacc actaaaattt
     2881 gtcttgtcaa agccaattcc aacgaaaatt ggattttttt tgacttaata tatattaacc
     2941 cttgaaattc ccttcaattg gaaaattttt ttaatttatt tatttcttct ttcttttttt
     3001 ctttttcttt ttctttcttt tattcatcac aattgaattc aaatcaatta acatcaacaa
     3061 actttacaat cgattcaaca agtttgtaca aaaaagctga acgagaaacg taaaatgata
     3121 taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa
     3181 cacaacatat ccagtcacta tggcggccgc attaggcacc ccaggcttta cactttatgc
     3241 ttccggctcg tataatgtgt ggattttgag ttaggatccg tcgagatttt caggagctaa
     3301 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
     3361 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
     3421 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
     3481 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat
     3541 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
     3601 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
     3661 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
     3721 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga
     3781 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta
     3841 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     3901 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg
     3961 cggggcgtaa agatctggat ccggcttact aaaagccaga taacagtatg cgtatttgcg
     4021 cgctgatttt tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag
     4081 aggtatgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc
     4141 aaggcatata tgatgtcaat atctccggtc tggtaagcac aaccatgcag aatgaagccc
     4201 gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc
     4261 ggtttattga aatgaacggc tcttttgctg acgagaacag gggctggtga aatgcagttt
     4321 aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat
     4381 attattgaca cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca
     4441 gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg
     4501 atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc
     4561 agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg
     4621 tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga tatgttgtgt
     4681 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat
     4741 atcattttac gtttctcgtt cagctttctt gtacaaagtg gttgatatca tgaataaata
     4801 gcattataag taaatgcatg tatactaaac tcacaaatta gagcttcaat ttaattatat
     4861 cagttattac ccgggaatct cggtcgtaat gatttctata atgacgaaaa aaaaaaattg
     4921 gaaagaaaaa gcttcatggc ctttataaaa aggaactatc caatacctcg ccagaaccaa
     4981 gtaacagtat tttacggggc acaaatcaag aacaataaga caggactgta aagatggacg
     5041 catagcagat cgaattcacg cgtatggcag taggtaaaaa taagagactt tctaaaggaa
     5101 agaaaggatt gaaaaagaag gtcgtcgacc cattcaccag aaaagattgg tttgacatta
     5161 aagccccaac cacctttgaa aacagaaatg ttggtaaaac tttaatcaac agatccactg
     5221 gtttgaagaa tgcagccgat ggattaaagg gaagagtttt cgaagtttgt ttggctgact
     5281 tgcaaggttc agaagaccac tcatacagaa agatcaaatt gagagttgat gaagttcaag
     5341 gtaaaaactt gttgaccaac ttccacggtt tggatttcac ctcagacaaa ataagatcaa
     5401 ggcctttggt tagaaaatgg caatcattgg ttgaagccaa tgttactgtc aagactgccg
     5461 atgactacgt tttgagagta tttgccattg ccttcaccaa gagacaacca aaccaaatca
     5521 aaaagaccac ttatgctcaa tcatccaaat tgagagaaat cagaaagaag atgactgaaa
     5581 tcatgcaaag agaagtttcc aactgtactt tggctcaatt aacctccaag ttgattccag
     5641 aagttattgg tcgtgaaatt gaaaaatcta ctcaaactat cttcccattg caaaacgttc
     5701 acatcagaaa agtcaaattg ttgaaacaac caaaattcga cttgggttca ttgttggctt
     5761 tgcacggtga aggttcaact gaagaaaagg gtaagaaggt ttcaggtggt ttcaaggatg
     5821 ttgttttgga atcagtttaa actagtcctt acctacccat tctagaaaga gccaatcaga
     5881 atcaagtgag gctaccatta cttcaaatag atttatttcc gaagatccaa gtaaaccggt
     5941 gagagaaatt tccgatattg aattatcgca atttttagaa gaatcggtac atttttaaaa
     6001 aaaagtattt aggtggtgtt cattttgtat taataatatt cgttaaattt tgtgtatact
     6061 ttacttaagc tttttctttt aagattttag taacagcttc gacaatcgcg tcaccaactt
     6121 cagtagctga actggtacct cttaaatctg cagttctgat acctttatcc aagacttgtt
     6181 tcacggcttc ttctaaagct tcagcttctt tcacacaatc caaagataat ctcaacatag
     6241 aagcagcaga taaaatagta gcaattgggt taactttatt ggctggtaaa tcaggagcag
     6301 aaccgtgaca tggttcataa agaccaaaag cggtgttggt atctggcaat gaagccaaag
     6361 atgcggatgg taataaaccc aaggaacctg gaataactga agcttcatcg gaaatgatat
     6421 caccaaacat gttagaagtg atgattatac cattcaattt ggttgggttt tgaattaaaa
     6481 tcatggcggc ggagtcaatc aattgatgtt gaacagacaa agttgggaac tcttcagaaa
     6541 tcactttatc gacagttctt ctccataatc tagaagaagc taaaacatta gccttatcca
     6601 atgaccagat tggtaatggt gggttatgtt gtaaagccat gaaggcagcc atacgggtga
     6661 ttctggtgac ttcatcgacg gtgtattttt cagtatccca tgcggtttgt ttatcttcac
     6721 tttcttcttg ttcttcacga tcaccgaaat aaataccacc aactaattca cgaacaatga
     6781 tcaagtttgt acctttaacg acttctggtc tcaatgggga taattccaat aaggagtcac
     6841 tggcaaagtt acatggtctg atgttagcat aaaggttcaa ttctttacga atctttaata
     6901 aaccttgttc tggacgaacg gcgccagtcc cccattttgg tccaccaaca gcacccaata
     6961 agacggcatc agattttttg gcactttcaa gtgcgtcatc tggcaatggg acaccagtgg
     7021 catcaatggc agcaccaccg attaaatgat gtttgaaatc gaaatggatt ttttggtaag
     7081 gagtggcagc ttcaattgct tctaaaactt tgatagcttc attgacaatt tcggtaccga
     7141 cgtgatcacc tggtaaaatt gtaatagttt tagttttaac tgacattgtg ggtttgggta
     7201 gtgaataaag gaattaagga tgaaggaaaa aaaattcttg agtgaaaaat ttcatagcct
     7261 gagtccctcg gatttttttc gtttattata tatttttata gtgaacaaga aaaaaaaaat
     7321 tgaacccggc tgcggaaccc acgcacgccg ttacaggagt tagtcattct tcaacaatac
     7381 gaacgtatta ttcatctggt tcagatacat gaacgaaagt acaatttccg aaattactct
     7441 atacacgata attacacata acaacacaaa ttacaaaaaa acaaaaaacc ttaatgtcca
     7501 ctccattcga tagagttcaa gtcaatacct tcttgaacca tttcccataa tggagacatc
     7561 tttctcaaga aatcaactct ttcattaatt gcttgaataa catagtcaac ttctgcttca
     7621 gtggtgaatc taccaatacc gaatctaatg gaagaatgag ccaaggcatc atcggcaccc
     7681 aaagcatgta aaacatacga tggttccaaa gatgcagagg tacatgcaga accagaactc
     7741 aatgcaatat ctttcaatgc cattaacaaa gattcccctt caatgtatgc aaaggaaaca
     7801 ttcacacaac ctgggtattg atatgttggg ttattacaac cgttaaactg agtagatgga
     7861 atggataaca aaccgttctt caatttagta gataacctct caatatgttt cttgtcaaat
     7921 gcagattctt gtttcattaa tcttgcagct tcaccaaacc cagctactaa tggtggtgcc
     7981 aaagtacctg atctcaaacc tctttcttga cccccaccag tgatgattgg atccaagggc
     8041 gaattccagc acactggcgg cgagctccaa ttcgccctat agtgagtcgt attacaattc
     8101 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     8161 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     8221 cccttcccaa cagttgcgca gcctgaatgg cgaatggaaa ttgtaagcgt taatattttg
     8281 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     8341 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     8401 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     8461 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     8521 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     8581 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     8641 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     8701 ctacagggcg cgtca
//
