LOCUS       V016182                 7318 bp    DNA     circular SYN 24-FEB-2021
DEFINITION  Exported.
ACCESSION   V016182
VERSION     V016182
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7318)
  AUTHORS   Grogan CL, Lampe DJ.
  TITLE     Novel Asaia sp. type II secretion signals for Plasmodium inhibition
            in Anopheles stephensi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7318)
  AUTHORS   Grogan CL, Lampe DJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2020) Biological Sciences, Duquesne University,
            600 Forbes Ave, Pittsburgh, PA 15219-3016, USA
REFERENCE   3  (bases 1 to 7318)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-OCT-2020) Biological Sciences, Duquesne University, 600 Forbes
            Ave, Pittsburgh, PA 15219-3016, USA"
FEATURES             Location/Qualifiers
     source          1..7318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             458..1249
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     regulatory      1552..1763
                     /label="PnptII"
                     /note="PnptII"
                     /regulatory_class="promoter"
     misc_feature    1767..1916
                     /label="dipeptidyl carboxypeptidase II secretion signal"
                     /note="dipeptidyl carboxypeptidase II secretion signal"
     CDS             1923..3272
                     /gene="phoA"
                     /label="Alkaline phosphatase"
                     /note="Alkaline phosphatase from Escherichia coli (strain
                     K12). Accession#: P00634"
     CDS             complement(4867..5526)
                     /label="pBBR1 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(5527..6296)
                     /direction=LEFT
                     /label="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1
                     Rep protein for replication"
ORIGIN
        1 accttcggga gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc
       61 aggccaaggc cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg
      121 aagtccagcg ccagaaacag gcccagcgcc agcaggaacg cgggcgcgca catttccccg
      181 aaaagtgcca cctgggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag
      241 cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt
      301 tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa
      361 gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc
      421 aagatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
      481 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
      541 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
      601 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
      661 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
      721 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
      781 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
      841 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
      901 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
      961 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     1021 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     1081 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     1141 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     1201 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     1261 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     1321 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     1381 atcctccagc gcggggatct catgctggag ttcttcgccc acccccatgg gcaaatatta
     1441 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     1501 tggcttccat gtcggcagaa tgcttaatga attacaacag tttttatgca taaccggaat
     1561 tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt
     1621 tcttgccgcc aaggatctga tggcgcaggg gatcaagatc tgatcaagag acaggatgtc
     1681 gactctagag gatccccggg taccgagctc gaattcggct tattccctaa ctaactaaag
     1741 attaacttta taaggaggaa aaacatatgc ccgttttcag acacactgcg gctctggcag
     1801 ctgttgccct cccccttctg tcttccacgg cgctggctgc ccctgccaac ccgctgtttc
     1861 aggcaagccc gctgccctat caggcacccc ccttcgacaa gatcaaggac agtgatttaa
     1921 ttaaaacctg caggatgcct gttctggaaa accgggctgc tcagggcgat attactgcac
     1981 ccggcggtgc tcgccgttta acgggtgatc agactgccgc tctgcgtgat tctcttagcg
     2041 ataaacctgc aaaaaatatt attttgctga ttggcgatgg gatgggggac tcggaaatta
     2101 ctgccgcacg taattatgcc gaaggtgcgg gcggcttttt taaaggtata gatgccttac
     2161 cgcttaccgg gcaatacact cactatgcgc tgaataaaaa aaccggcaaa ccggactacg
     2221 tcaccgactc ggctgcatca gcaaccgcct ggtcaaccgg tgtcaaaacc tataacggcg
     2281 cgctgggcgt cgatattcac gaaaaagatc acccaacgat tctggaaatg gcaaaagccg
     2341 caggtctggc gaccggtaac gtttctaccg cagagttgca ggatgccacg cccgctgcgc
     2401 tggtggcaca tgtgacctcg cgcaaatgct acggtccgag cgcgaccagt gaaaaatgtc
     2461 cgggtaacgc tctggaaaaa ggcggaaaag gatcgattac cgaacagctg cttaacgctc
     2521 gtgccgacgt tacgcttggc ggcggcgcaa aaacctttgc tgaaacggca accgctggtg
     2581 aatggcaggg aaaaacgctg cgtgaacagg cacaggcgcg tggttatcag ttggtgagcg
     2641 atgctgcctc actgaattcg gtgacggaag cgaatcagca aaaacccctg cttggcctgt
     2701 ttgctgacgg caatatgcca gtgcgctggc taggaccgaa agcaacgtac catggcaata
     2761 tcgataagcc cgcagtcacc tgtacgccaa atccgcaacg taatgacagt gtaccaaccc
     2821 tggcgcagat gaccgacaaa gccattgaat tgttgagtaa aaatgagaaa ggctttttcc
     2881 tgcaagttga aggtgcgtca atcgataaac aggatcatgc tgcgaatcct tgtgggcaaa
     2941 ttggcgagac ggtcgatctc gatgaagccg tacaacgggc gctggaattc gctaaaaagg
     3001 agggtaacac gctggtcata gtcaccgctg atcacgccca cgccagccag attgttgcgc
     3061 cggataccaa agctccgggc ctcacccagg cgctaaatac caaagatggc gcagtgatgg
     3121 tgatgagtta cgggaactcc gaagaggatt cacaagaaca taccggcagt cagttgcgta
     3181 ttgcggcgta tggcccgcat gccgccaatg ttgttggact gaccgaccag accgatctct
     3241 tctacaccat gaaagccgct ctggggctga aataaaaccg cgcccggcag tgaattttcg
     3301 ctgccgggtg gtttttttgc tgttagcaac cagacttaat ggcagatcac gggcgcatac
     3361 gctcatggtt aaaacatgaa gagggatggt gctatgaaaa taacattact ggttaccttg
     3421 cttttcggtc tggttttttt aaccaccgtc ggcgctgccg agagaacttt aaccccacaa
     3481 caacagcgta tgacctcctg taatcagcag gcgacggcgc aggcgttgaa aggggatgct
     3541 cgtaagacct acatgagtga ttgcctgaag aacagcaagt ctgcgcctgg cgaaaaaagt
     3601 ttgacgccac agcagcaaaa gatgcgcgaa tgcaataatc aagcaacaca acaatctctg
     3661 aaaggtgatg atcgtaataa gtttatgagt gcctgcctca agaaagccgc ctgatacctg
     3721 atagtgctaa cgggtgagct acgaaaatgg ctcacccgaa atatcatact tctgccttta
     3781 gctccgtctc tataatttgg gaaaattgtt tctgaatgtt cccaaaaata atgaatgatg
     3841 aaaacttttt caaaaaagcg gcggcgcacg gggaggaacc tcctttaact cctcaaaacg
     3901 aacatcagcg gtccgggctg cgcttcgccc gtcgcgtcag actaccccgt gcggttggcc
     3961 tggctggcat gttcttaccg attgcttcaa cgctggtttc acacccgccg ccgggctggt
     4021 ggtggctggt gttggtcggc tgggcgttcg tctggccgca tttagcctgg cagatagcga
     4081 gcagggccgt cgatccgctt agccgggaaa tttacaactt aaaaaccgat gcagtattag
     4141 cgggaatgtg ggtaggcgta atgggcgtaa acgtgctgcc ttccaccgcg atgttgatga
     4201 ttatgtgtct gaatttgatg ggggcaggcg gcccccgtct gtttgtcgcg ggtctggtgt
     4261 tgatggtggt ttcctgcctt gtcaccggcc ggccggatcc gaattcgcgt taatattttg
     4321 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgactgc
     4381 gatgagtggc agggcggggc gtaatttttt taaggcagtt attggtgccc ttaaacgcct
     4441 ggtgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt
     4501 cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt
     4561 taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca
     4621 gtttgctcag gctctccccg tggaggtaat aattgacgat atgatcattt attctgcctc
     4681 ccagagcctg ataaaaacgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc
     4741 gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg
     4801 gcgaggcggc tacagccgat agtctggaac agcgcactta cgggttgctg cgcaacccaa
     4861 gtgctaccgg cgcggcagcg tgacccgtgt cggcggctcc aacggctcgc catcgtccag
     4921 aaaacacggc tcatcgggca tcggcaggcg ctgctgcccg cgccgttccc attcctccgt
     4981 ttcggtcaag gctggcaggt ctggttccat gcccggaatg ccgggctggc tgggcggctc
     5041 ctcgccgggg ccggtcggta gttgctgctc gcccggatac agggtcggga tgcggcgcag
     5101 gtcgccatgc cccaacagcg attcgtcctg gtcgtcgtga tcaaccacca cggcggcact
     5161 gaacaccgac aggcgcaact ggtcgcgggg ctggccccac gccacgcggt cattgaccac
     5221 gtaggccgac acggtgccgg ggccgttgag cttcacgacg gagatccagc gctcggccac
     5281 caagtccttg actgcgtatt ggaccgtccg caaagaacgt ccgatgagct tggaaagtgt
     5341 cttctggctg accaccacgg cgttctggtg gcccatctgc gccacgaggt gatgcagcag
     5401 cattgccgcc gtgggtttcc tcgcaataag cccggcccac gcctcatgcg ctttgcgttc
     5461 cgtttgcacc cagtgaccgg gcttgttctt ggcttgaatg ccgatttctc tggactgcgt
     5521 ggccatgctt atctccatgc ggtagggtgc cgcacggttg cggcaccatg cgcaatcagc
     5581 tgcaactttt cggcagcgcg acaacaatta tgcgttgcgt aaaagtggca gtcaattaca
     5641 gattttcttt aacctacgca atgagctatt gcggggggtg ccgcaatgag ctgttgcgta
     5701 cccccctttt ttaagttgtt gatttttaag tctttcgcat ttcgccctat atctagttct
     5761 ttggtgccca aagaagggca cccctgcggg gttcccccac gccttcggcg cggctccccc
     5821 tccggcaaaa agtggcccct ccggggcttg ttgatcgact gcgcggcctt cggccttgcc
     5881 caaggtggcg ctgccccctt ggaacccccg cactcgccgc cgtgaggctc ggggggcagg
     5941 cgggcgggct tcgccttcga ctgcccccac tcgcataggc ttgggtcgtt ccaggcgcgt
     6001 caaggccaag ccgctgcgcg gtcgctgcgc gagccttgac ccgccttcca cttggtgtcc
     6061 aaccggcaag cgaagcgcgc aggccgcagg ccggaggctt ttccccagag aaaattaaaa
     6121 aaattgatgg ggcaaggccg caggccgcgc agttggagcc ggtgggtatg tggtcgaagg
     6181 ctgggtagcc ggtgggcaat ccctgtggtc aagctcgtgg gcaggcgcag cctgtccatc
     6241 agcttgtcca gcagggttgt ccacgggccg agcgaagcga gccagccggt ggccgctcgc
     6301 ggccatcgtc cacatatcca cgggctggca agggagcgca gcgaccgcgc agggcgaagc
     6361 ccggagagca agcccgtagg gcgccgcagc cgccgtaggc ggtcacgact ttgcgaagca
     6421 aagtctagtg agtatactca agcattgagt ggcccgccgg aggcaccgcc ttgcgctgcc
     6481 cccgtcgagc cggttggaca ccaaaaggga ggggcaggca tggcggcata cgcgatcatg
     6541 cgatgcaaga agctggcgaa aatgggcaac gtggcggcca gtctcaagca cgcctaccgc
     6601 gagcgcgaga cgcccaacgc tgacgccagc aggacgccag agaacgagca ctgggcggcc
     6661 agcagcaccg atgaagcgat gggccgactg cgcgagttgc tgccagagaa gcggcgcaag
     6721 gacgctgtgt tggcggtcga gtacgtcatg acggccagcc cggaatggtg gaagtcggcc
     6781 agccaagaac agcaggcggc gttcttcgag aaggcgcaca agtggctggc ggacaagtac
     6841 ggggcggatc gcatcgtgac ggccagcatc caccgtgacg aaaccagccc gcacatgacc
     6901 gcgttcgtgg tgccgctgac gcaggacggc aggctgtcgg ccaaggagtt catcggcaac
     6961 aaagcgcaga tgacccgcga ccagaccacg tttgcggccg ctgtggccga tctagggctg
     7021 caacggggca tcgagggcag caaggcacgt cacacgcgca ttcaggcgtt ctacgaggcc
     7081 ctggagcggc caccagtggg ccacgtcacc atcagcccgc aagcggtcga gccacgcgcc
     7141 tatgcaccgc agggattggc cgaaaagctg ggaatctcaa agcgcgttga gacgccggaa
     7201 gccgtggccg accggctgac aaaagcggtt cggcaggggt atgagcctgc cctacaggcc
     7261 gccgcaggag cgcgtgagat gcgcaagaag gccgatcaag cccaagagac ggcccgag
//
