LOCUS       62056_8280        4681 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4681)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4681
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_signal     190..340
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     promoter        462..990
                     /label=mCMV promoter
                     /note="mouse cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             1005..1712
                     /codon_start=1
                     /label=mNeonGreen
                     /note="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     CDS             1713..1766
                     /codon_start=1
                     /label=T2A
                     /note="2A peptide from Thosea asigna virus capsid protein"
                     /translation="EGRGSLLTCGDVEENPGP"
     polyA_signal    1804..1938
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(1958..1991)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(2013..2029)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2037..2053)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2061..2091)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2106..2127)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2415..3003)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3177..4034)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4035..4139)
                     /label=AmpR promoter
     repeat_region   4681
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
ORIGIN
        1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt
       61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg
      121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg
      181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt
      241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa
      301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg
      361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc
      421 gggtcaaagt tggcgtttta ttattatagt cagntctaga gatatactga gtcattaggg
      481 actttccaat gggttttgcc cagtacataa ggtcaatagg ggtgaatcaa caggaaagtc
      541 ccattggagc caagtacact gagtcaatag ggactttcca ttgggttttg cccagtacaa
      601 aaggtcaata gggggtgagt caatgggttt ttcccattat tggcacgtac ataaggtcaa
      661 taggggtgag tcattgggtt tttccagcca tttaattaaa acgccatgta ctttcccacc
      721 attgacgtca atgggctatt gaaactaatg caacgtgacc tttaaacggt actttcccat
      781 agctgattaa tgggaaagta ccgttctcga gccaatacac gtcaatggga agtgaaaggg
      841 cagccaaaac gtaacaccgc cccggttttc ccctggaaat tccatattgg cactcattct
      901 attggctgag ctgcgttcta cgtgggtata agaggcgcga ccagcgtcgg taccgtcgca
      961 gtcttcggtc tgaccaccgt agaacgcaga tcgaattcgc caccatggtg agcaagggcg
     1021 aggaggataa catggcctct ctcccagcga cacatgagtt acacatcttt ggctccatca
     1081 acggtgtgga ctttgacatg gtgggtcagg gcaccggcaa tccaaatgat ggttatgagg
     1141 agttaaacct gaagtccacc aagggtgacc tccagttctc cccctggatt ctggtccctc
     1201 atatcgggta tggcttccat cagtacctgc cctaccctga cgggatgtcg cctttccagg
     1261 ccgccatggt agatggctcc ggataccaag tccatcgcac aatgcagttt gaagatggtg
     1321 cctcccttac tgttaactac cgctacacct acgagggaag ccacatcaaa ggagaggccc
     1381 aggtgaaggg gactggtttc cctgctgacg gtcctgtgat gaccaactcg ctgaccgctg
     1441 cggactggtg caggtcgaag aagacttacc ccaacgacaa aaccatcatc agtaccttta
     1501 agtggagtta caccactgga aatggcaagc gctaccggag cactgcgcgg accacctaca
     1561 cctttgccaa gccaatggcg gctaactatc tgaagaacca gccgatgtac gtgttccgta
     1621 agacggagct caagcactcc aagaccgagc tcaacttcaa ggagtggcaa aaggccttta
     1681 ccgatgtgat gggcatggac gagctgtaca aggagggcag aggaagtctt ctaacatgcg
     1741 gtgacgtgga ggagaatccc ggccctcccg ggagatctaa gcttgagctc gtcgacttcg
     1801 agcaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     1861 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     1921 tcttatcatg tctggatcgt ctagcatcga agatccaata acttcgtata gcatacatta
     1981 tacgaagtta taagtagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     2041 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     2101 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     2161 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     2221 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     2281 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     2341 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     2401 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     2461 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     2521 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2581 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg
     2641 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     2701 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     2761 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     2821 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     2881 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     2941 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     3001 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     3061 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     3121 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     3181 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     3241 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     3301 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     3361 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     3421 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     3481 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     3541 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     3601 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     3661 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     3721 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     3781 ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     3841 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     3901 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     3961 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     4021 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     4081 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     4141 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     4201 tataaaaata ggcgtatcac tctaggcaaa atagcaccct cccgctccag aacaacatac
     4261 agcgcttcac agcggcagcc taacagtcag ccttaccagt aaaaaagaaa acctattaaa
     4321 aaaacaccac tcgacacggc accagctcaa tcagtcacag tgtaaaaaag ggccaagtgc
     4381 agagcgagta tatataggac taaaaaatga cgtaacggtt aaagtccaca aaaaacaccc
     4441 agaaaaccgc acgcgaacct acgcccagaa acgaaagcca aaaaacccac aacttcctca
     4501 aatcgtcact tccgttttcc cacgttacgt aacttcccat tttaagaaaa ctacaattcc
     4561 caacacatac aagttactcc gccctaaaac ctacgtcacc cgccccgttc ccacgccccg
     4621 cgccacgtca caaactccac cccctcatta tcatattggc ttcaatccaa aataaggtat
     4681 a
//
