LOCUS       V013613                 6740 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013613
VERSION     V013613
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6740)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6740
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_signal     190..340
                     /label="Ad5 Psi"
                     /note="packaging signal for adenovirus serotype 5"
     promoter        461..990
                     /label="mCMV promoter"
                     /note="mouse cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             999..1715
                     /label="EGFP"
                     /note="enhanced GFP"
     CDS             1734..2582
                     /gene="MTAP"
                     /label="S-methyl-5'-thioadenosine phosphorylase"
                     /note="S-methyl-5'-thioadenosine phosphorylase from Homo
                     sapiens. Accession#: Q13126"
     polyA_signal    2723..2844
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     enhancer        2852..3231
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3232..3435
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        3480..3498
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3625..3849
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    3863..3997
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(4017..4050)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(4072..4088)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4096..4112)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4120..4150)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4165..4186)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4474..5062)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5236..6093)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6094..6198)
                     /label="AmpR promoter"
     repeat_region   6740
                     /label="ITR"
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
ORIGIN
        1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt
       61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg
      121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg
      181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt
      241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa
      301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg
      361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc
      421 gggtcaaagt tggcgtttta ttattatagt cagtctagag atatactgag tcattaggga
      481 ctttccaatg ggttttgccc agtacataag gtcaataggg gtgaatcaac aggaaagtcc
      541 cattggagcc aagtacactg agtcaatagg gactttccat tgggttttgc ccagtacaaa
      601 aggtcaatag ggggtgagtc aatgggtttt tcccattatt ggcacgtaca taaggtcaat
      661 aggggtgagt cattgggttt ttccagccaa tttaattaaa acgccatgta ctttcccacc
      721 attgacgtca atgggctatt gaaactaatg caacgtgacc tttaaacggt actttcccat
      781 agctgattaa tgggaaagta ccgttctcga gccaatacac gtcaatggga agtgaaaggg
      841 cagccaaaac gtaacaccgc cccggttttc ccctggaaat tccatattgg cacgcattct
      901 attggctgag ctgcgttcta cgtgggtata agaggcgcga ccagcgtcgg taccgtcgca
      961 gtcttcggtc tgaccaccgt agaacgcaga tcgaattcat ggtgagcaag ggcgaggagc
     1021 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     1081 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     1141 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     1201 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     1261 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     1321 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     1381 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     1441 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     1501 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     1561 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     1621 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     1681 ccgggatcac tctcggcatg gacgagctgt acaagtccgg aggcggaggg agtatggcct
     1741 ctggcaccac caccaccgcc gtgaagattg gaataattgg tggaacaggc ctggatgatc
     1801 cagaaatttt agaaggaaga actgaaaaat atgtggatac tccatttggc aagccatctg
     1861 atgccttaat tttggggaag ataaaaaatg ttgattgcgt cctccttgca aggcatggaa
     1921 ggcagcacac catcatgcct tcaaaggtca actaccaggc gaacatctgg gctttgaagg
     1981 aagagggctg tacacatgtc atagtgacca cagcttgtgg ctccttgagg gaggagattc
     2041 agcccggcga tattgtcatt attgatcagt tcattgacag gaccactatg agacctcagt
     2101 ccttctatga tggaagtcat tcttgtgcca gaggagtgtg ccatattcca atggctgagc
     2161 cgttttgccc caaaacgaga gaggttctta tagagactgc taagaagcta ggactccggt
     2221 gccactcaaa ggggacaatg gtcacaatcg agggacctcg ttttagctcc cgggcagaaa
     2281 gcttcatgtt ccgcacctgg ggggcggatg ttatcaacat gaccacagtt ccagaggtgg
     2341 ttcttgctaa ggaggctgga atttgttacg caagtatcgc catggcgaca gattatgact
     2401 gctggaagga gcacgaggaa gcagtttcgg tggaccgggt cttaaagacc ctgaaagaaa
     2461 acgctaataa agccaaaagc ttactgctca ctaccatacc tcagataggg tccacagaat
     2521 ggtcagaaac cctccataac ctgaagaata tggcccagtt ttctgtttta ttaccaagac
     2581 attaaagatc tcgagatcca ccggatctag ataactgatc ataatcagcc ataccacatt
     2641 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     2701 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     2761 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     2821 gtccaaactc atcaatgtat cttaacgcgt tgacattgat tattgactag ttattaatag
     2881 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     2941 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     3001 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     3061 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
     3121 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
     3181 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
     3241 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
     3301 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
     3361 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
     3421 tatataagca gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt
     3481 aatacgactc actataggga gacccaagct ggctagcgtt taaacgggcc ctctagactc
     3541 gagcggccgc cactgtgctg gatatctgca gaattgatcc gagctcggta ccaagcttaa
     3601 gtttaaaccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     3661 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     3721 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     3781 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     3841 gctctatggg tcgacttcga gcaacttgtt tattgcagct tataatggtt acaaataaag
     3901 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     3961 gtccaaactc atcaatgtat cttatcatgt ctggatcgtc tagcatcgaa gatccaataa
     4021 cttcgtatag catacattat acgaagttat aagtagcttg gcgtaatcat ggtcatagct
     4081 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     4141 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     4201 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     4261 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     4321 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     4381 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     4441 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     4501 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     4561 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     4621 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg
     4681 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     4741 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     4801 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     4861 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     4921 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     4981 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     5041 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     5101 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     5161 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     5221 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     5281 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     5341 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     5401 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     5461 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     5521 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     5581 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     5641 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     5701 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     5761 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     5821 gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac
     5881 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     5941 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     6001 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     6061 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     6121 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     6181 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     6241 tattatcatg acattaacct ataaaaatag gcgtatcact ctaggcaaaa tagcaccctc
     6301 ccgctccaga acaacataca gcgcttcaca gcggcagcct aacagtcagc cttaccagta
     6361 aaaaagaaaa cctattaaaa aaacaccact cgacacggca ccagctcaat cagtcacagt
     6421 gtaaaaaagg gccaagtgca gagcgagtat atataggact aaaaaatgac gtaacggtta
     6481 aagtccacaa aaaacaccca gaaaaccgca cgcgaaccta cgcccagaaa cgaaagccaa
     6541 aaaacccaca acttcctcaa atcgtcactt ccgttttccc acgttacgta acttcccatt
     6601 ttaagaaaac tacaattccc aacacataca agttactccg ccctaaaacc tacgtcaccc
     6661 gccccgttcc cacgccccgc gccacgtcac aaactccacc ccctcattat catattggct
     6721 tcaatccaaa ataaggtata
//
