LOCUS       62056_8265        4673 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4673
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_signal     190..340
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     promoter        462..991
                     /label=mCMV promoter
                     /note="mouse cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             1000..1674
                     /codon_start=1
                     /label=DsRed2
                     /note="improved tetrameric variant of DsRed fluorescent
                     protein"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     CDS             1708..1731
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /translation="DYKDDDDK"
     CDS             1732..1755
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /translation="DYKDDDDK"
     CDS             1756..1779
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /translation="DYKDDDDK"
     polyA_signal    1796..1930
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(1950..1983)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(2005..2021)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2029..2045)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2053..2083)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2098..2119)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2407..2995)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3169..4026)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4027..4131)
                     /label=AmpR promoter
     repeat_region   4673
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
ORIGIN
        1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt
       61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg
      121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg
      181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt
      241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa
      301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg
      361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc
      421 gggtcaaagt tggcgtttta ttattatagt cagctctaga gatatactga gtcattaggg
      481 actttccaat gggttttgcc cagtacataa ggtcaatagg ggtgaatcaa caggaaagtc
      541 ccattggagc caagtacact gagtcaatag ggactttcca ttgggttttg cccagtacaa
      601 aaggtcaata gggggtgagt caatgggttt ttcccattat tggcacgtac ataaggtcaa
      661 taggggtgag tcattgggtt tttccagcca atttaattaa aacgccatgt actttcccac
      721 cattgacgtc aatgggctat tgaaactaat gcaacgtgac ctttaaacgg tactttccca
      781 tagctgatta atgggaaagt accgttctcg agccaataca cgtcaatggg aagtgaaagg
      841 gcagccaaaa cgtaacaccg ccccggtttt cccctggaaa ttccatattg gcacgcattc
      901 tattggctga gctgcgttct acgtgggtat aagaggcgcg accagcgtcg gtaccgtcgc
      961 agtcttcggt ctgaccaccg tagaacgcag atcgaattca tggcctcctc cgagaacgtc
     1021 atcaccgagt tcatgcgctt caaggtgcgc atggagggca ccgtgaacgg ccacgagttc
     1081 gagatcgagg gcgagggcga gggccgcccc tacgagggcc acaacaccgt gaagctgaag
     1141 gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttccagtac
     1201 ggctccaagg tgtacgtgaa gcaccccgcc gacatccccg actacaagaa gctgtccttc
     1261 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggcgaccgtg
     1321 acccaggact cctccctgca ggacggctgc ttcatctaca aggtgaagtt catcggcgtg
     1381 aacttcccct ccgacggccc cgtgatgcag aagaagacca tgggctggga ggcctccacc
     1441 gagcgcctgt acccccgcga cggcgtgctg aagggcgaga cccacaaggc cctgaagctg
     1501 aaggacggcg gccactacct ggtggagttc aagtccatct acatggccaa gaagcccgtg
     1561 cagctgcccg gctactacta cgtggacgcc aagctggaca tcacctccca caacgaggac
     1621 tacaccatcg tggagcagta cgagcgcacc gagggccgcc accacctgtt cctgggagga
     1681 gggggaagca agcttgagct cggatccgat tacaaggatg acgacgataa ggactataag
     1741 gacgatgatg acaaggacta caaagatgat gacgataaat gagtcgactt cgagcaactt
     1801 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     1861 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     1921 tgtctggatc gtctagcatc gaagatccaa taacttcgta tagcatacat tatacgaagt
     1981 tataagtagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     2041 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     2101 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     2161 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     2221 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     2281 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     2341 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     2401 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     2461 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     2521 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     2581 gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     2641 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     2701 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     2761 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     2821 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     2881 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     2941 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     3001 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     3061 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     3121 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     3181 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     3241 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     3301 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     3361 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     3421 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     3481 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     3541 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     3601 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     3661 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     3721 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     3781 aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     3841 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     3901 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     3961 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     4021 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     4081 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     4141 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     4201 taggcgtatc actctaggca aaatagcacc ctcccgctcc agaacaacat acagcgcttc
     4261 acagcggcag cctaacagtc agccttacca gtaaaaaaga aaacctatta aaaaaacacc
     4321 actcgacacg gcaccagctc aatcagtcac agtgtaaaaa agggccaagt gcagagcgag
     4381 tatatatagg actaaaaaat gacgtaacgg ttaaagtcca caaaaaacac ccagaaaacc
     4441 gcacgcgaac ctacgcccag aaacgaaagc caaaaaaccc acaacttcct caaatcgtca
     4501 cttccgtttt cccacgttac gtaacttccc attttaagaa aactacaatt cccaacacat
     4561 acaagttact ccgccctaaa acctacgtca cccgccccgt tcccacgccc cgcgccacgt
     4621 cacaaactcc accccctcat tatcatattg gcttcaatcc aaaataaggt ata
//
