LOCUS       V047609                 4778 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V047609
VERSION     V047609
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_signal     190..340
                     /note="packaging signal for adenovirus serotype 5"
                     /label="Ad5 Ψ"
     promoter        470..993
                     /label="mCMV promoter"
     CDS             1009..1080
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags"
                     /transl_table=1
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
                     /label="3xFLAG"
     CDS             1093..1884
                     /label="Cdca5(NM_026410)"
                     /note="Cdca5(NM_026410)"
                     /gene="Cdca5"
     polyA_signal    1901..2035
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     protein_bind    complement(2055..2088)
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
                     /label="loxP"
     primer_bind     complement(2110..2126)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    2134..2150
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(2158..2188)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    2203..2224
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(2512..3100)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(3271..4131)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(4132..4236)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt
       61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg
      121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg
      181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt
      241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa
      301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg
      361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc
      421 gggtcaaagt tggcgtttta ttattatagt cagctctaga gatatactga gtcattaggg
      481 actttccaat gggttttgcc cagtacataa ggtcaatagg ggtgaatcaa caggaaagtc
      541 ccattggagc caagtacact gagtcaatag ggactttcca ttgggttttg cccagtacaa
      601 aaggtcaata gggggtgagt caatgggttt ttcccattat tggcacgtac ataaggtcaa
      661 taggggtgag tcattgggtt tttccagcca atttaattaa aacgccatgt actttcccac
      721 cattgacgtc aatgggctat tgaaactaat gcaacgtgac ctttaaacgg tactttccca
      781 tagctgatta atgggaaagt accgttctcg agccaataca cgtcaatggg aagtgaaagg
      841 gcagccaaaa cgtaacaccg ccccggtttt cccctggaaa ttccatattg gcacgcattc
      901 tattggctga gctgcgttct acgtgggtat aagaggcgcg accagcgtcg gtaccgtcgc
      961 agtcttcggt ctgaccaccg tagaacgcag atcgaattcc ccgggatgga ttacaaggat
     1021 gacgacgata aggactataa ggacgatgat gacaaggact acaaagatga tgacgataaa
     1081 agatctaagc ttatggcgga gcggcgaaca cgatcaggag gggctgccca gcggtctggg
     1141 cccaggactt cacttactaa gccttcgaag tcgtccaagc ggaaatctgg ctcagatctc
     1201 ccgaactcgt tctctgaaat ctggccgagg acaactccag cggtcccagt cagaaaggcc
     1261 atcgtcttga agaagatcgt agctcatgca gtagaggtcc cagatgtcca cacggttcga
     1321 agaagtccta ggatctcttt tattttggaa aaagagaaca accctccgct caaggtaccc
     1381 acgaaagaag acctcttcaa gacttgtagt gtccctggta ctccctccag cacccccgtg
     1441 ctgtacactc agaatgttga gcctgactct ggagaagcag agctggacag cagagacctg
     1501 gaaatgtctc agaaagtcag gcggtcgtac agtcggctac agagccttgg ttgtgcctca
     1561 acctccaccc ctggccgccg atccttcttt ggctttgagg gacctgacga cttgcctgga
     1621 gtctcacctg tcgtgtgttc aaagttaatc gagaccccaa aggtacctgc aaaggacttg
     1681 gttccggcta gaacgaagga cttggttcca gatagtacga aggacttggt tccggctaga
     1741 acgctccctg gaatctctcc cccagttgtg aaagagaaac gaaagaagaa ggttcctgaa
     1801 atcctgaaat cagagctgga taagtgggct gtggccatga atgctgaatt tgaagctgcc
     1861 gagcagtttg agcttctgat tgaatgagtc gacttcgagc aacttgttta ttgcagctta
     1921 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     1981 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcgtcta
     2041 gcatcgaaga tccaataact tcgtatagca tacattatac gaagttataa gtagcttggc
     2101 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     2161 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     2221 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     2281 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     2341 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     2401 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     2461 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     2521 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     2581 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     2641 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     2701 ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     2761 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     2821 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     2881 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     2941 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     3001 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     3061 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     3121 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     3181 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     3241 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     3301 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     3361 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     3421 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     3481 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     3541 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     3601 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     3661 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     3721 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     3781 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     3841 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac
     3901 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     3961 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     4021 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     4081 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     4141 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     4201 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     4261 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcactct
     4321 aggcaaaata gcaccctccc gctccagaac aacatacagc gcttcacagc ggcagcctaa
     4381 cagtcagcct taccagtaaa aaagaaaacc tattaaaaaa acaccactcg acacggcacc
     4441 agctcaatca gtcacagtgt aaaaaagggc caagtgcaga gcgagtatat ataggactaa
     4501 aaaatgacgt aacggttaaa gtccacaaaa aacacccaga aaaccgcacg cgaacctacg
     4561 cccagaaacg aaagccaaaa aacccacaac ttcctcaaat cgtcacttcc gttttcccac
     4621 gttacgtaac ttcccatttt aagaaaacta caattcccaa cacatacaag ttactccgcc
     4681 ctaaaaccta cgtcacccgc cccgttccca cgccccgcgc cacgtcacaa actccacccc
     4741 ctcattatca tattggcttc aatccaaaat aaggtata
//
