LOCUS       62056_8250        3989 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_signal     190..340
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     promoter        462..991
                     /label=mCMV promoter
                     /note="mouse cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             1009..1080
                     /codon_start=1
                     /label=3xFLAG
                     /note="three tandem FLAG(R) epitope tags"
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     polyA_signal    1112..1246
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(1266..1299)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(1321..1337)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1345..1361)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1369..1399)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1414..1435)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1723..2311)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2485..3342)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3343..3447)
                     /label=AmpR promoter
     repeat_region   3989
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
ORIGIN
        1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt
       61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg
      121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg
      181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt
      241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa
      301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg
      361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc
      421 gggtcaaagt tggcgtttta ttattatagt cagctctaga gatatactga gtcattaggg
      481 actttccaat gggttttgcc cagtacataa ggtcaatagg ggtgaatcaa caggaaagtc
      541 ccattggagc caagtacact gagtcaatag ggactttcca ttgggttttg cccagtacaa
      601 aaggtcaata gggggtgagt caatgggttt ttcccattat tggcacgtac ataaggtcaa
      661 taggggtgag tcattgggtt tttccagcca atttaattaa aacgccatgt actttcccac
      721 cattgacgtc aatgggctat tgaaactaat gcaacgtgac ctttaaacgg tactttccca
      781 tagctgatta atgggaaagt accgttctcg agccaataca cgtcaatggg aagtgaaagg
      841 gcagccaaaa cgtaacaccg ccccggtttt cccctggaaa ttccatattg gcacgcattc
      901 tattggctga gctgcgttct acgtgggtat aagaggcgcg accagcgtcg gtaccgtcgc
      961 agtcttcggt ctgaccaccg tagaacgcag atcgaattcc ccgggatgga ttacaaggat
     1021 gacgacgata aggactataa ggacgatgat gacaaggact acaaagatga tgacgataaa
     1081 agatctaagc ttgagctcgt cgacttcgag caacttgttt attgcagctt ataatggtta
     1141 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     1201 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcgtct agcatcgaag
     1261 atccaataac ttcgtatagc atacattata cgaagttata agtagcttgg cgtaatcatg
     1321 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     1381 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     1441 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     1501 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     1561 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     1621 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     1681 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     1741 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     1801 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     1861 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg
     1921 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     1981 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2041 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     2101 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     2161 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     2221 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     2281 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     2341 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     2401 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     2461 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     2521 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     2581 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     2641 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     2701 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     2761 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     2821 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     2881 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     2941 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     3001 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     3061 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca
     3121 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     3181 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     3241 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     3301 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     3361 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     3421 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     3481 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcactc taggcaaaat
     3541 agcaccctcc cgctccagaa caacatacag cgcttcacag cggcagccta acagtcagcc
     3601 ttaccagtaa aaaagaaaac ctattaaaaa aacaccactc gacacggcac cagctcaatc
     3661 agtcacagtg taaaaaaggg ccaagtgcag agcgagtata tataggacta aaaaatgacg
     3721 taacggttaa agtccacaaa aaacacccag aaaaccgcac gcgaacctac gcccagaaac
     3781 gaaagccaaa aaacccacaa cttcctcaaa tcgtcacttc cgttttccca cgttacgtaa
     3841 cttcccattt taagaaaact acaattccca acacatacaa gttactccgc cctaaaacct
     3901 acgtcacccg ccccgttccc acgccccgcg ccacgtcaca aactccaccc cctcattatc
     3961 atattggctt caatccaaaa taaggtata
//
