LOCUS       40924_14215        3287 bp DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3287
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_signal     190..340
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     protein_bind    complement(525..558)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      complement(1021..1609)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1783..2640)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2641..2745)
                     /label=AmpR promoter
     repeat_region   3287
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
ORIGIN
        1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt
       61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg
      121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg
      181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt
      241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa
      301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg
      361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc
      421 gggtcaaagt tggcgtttta ttattatagt cagntctaga gtcgacgagc tcaagcttag
      481 atctgctagc aggcctgaat tcggatcctc gactcgaaga tccaataact tcgtatagca
      541 tacattatac gaagttataa gtgccgccct ataccttgtc tgcctccccg cgttgcgtcg
      601 cggtgcatgg agccgggcca cctcgacctg aatggaagcc ggcggcacct cgctaacgga
      661 ttcaccactc caagaattgg agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc
      721 aacccttggc agaacatatc catcgcgtcc gccatctcca gcagccgcac gcggcgcatc
      781 tcggctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
      841 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
      901 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
      961 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     1021 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     1081 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     1141 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     1201 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     1261 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     1321 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     1381 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     1441 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     1501 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1561 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1621 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     1681 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     1741 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     1801 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     1861 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     1921 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     1981 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     2041 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     2101 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     2161 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     2221 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     2281 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     2341 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg
     2401 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     2461 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     2521 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     2581 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     2641 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     2701 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     2761 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     2821 tatcactcta ggcaaaatag caccctcccg ctccagaaca acatacagcg cttcacagcg
     2881 gcagcctaac agtcagcctt accagtaaaa aagaaaacct attaaaaaaa caccactcga
     2941 cacggcacca gctcaatcag tcacagtgta aaaaagggcc aagtgcagag cgagtatata
     3001 taggactaaa aaatgacgta acggttaaag tccacaaaaa acacccagaa aaccgcacgc
     3061 gaacctacgc ccagaaacga aagccaaaaa acccacaact tcctcaaatc gtcacttccg
     3121 ttttcccacg ttacgtaact tcccatttta agaaaactac aattcccaac acatacaagt
     3181 tactccgccc taaaacctac gtcacccgcc ccgttcccac gccccgcgcc acgtcacaaa
     3241 ctccaccccc tcattatcat attggcttca atccaaaata aggtata
//
