LOCUS       V008061                 6458 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008061
VERSION     V008061
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6458)
  AUTHORS   Crivat G, Tokumasu F, Sa JM, Hwang J, Wellems TE.
  TITLE     Tetracysteine-Based Fluorescent Tags to Study Protein Localization
            and Trafficking in Plasmodium falciparum-Infected Erythrocytes
  JOURNAL   PLoS ONE 6 (8), E22975 (2011)
   PUBMED   21860664
REFERENCE   2  (bases 1 to 6458)
  AUTHORS   Crivat G, Tokumasu F, Sa JM, Hwang J, Wellems TE.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-FEB-2011) LMVR, NIAID, 12735 Twinbrook Pkwy, 3E10C,
            Rockville, MD 20852, USA
REFERENCE   3  (bases 1 to 6458)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6458)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2011"; volume: "6"; issue: "8"; pages: "E22975"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (23-FEB-2011) LMVR, NIAID, 12735 Twinbrook Pkwy, 3E10C, Rockville,
            MD 20852, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6458
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(878..895)
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             complement(896..925)
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             complement(941..958)
                     /label="tetracysteine tag"
                     /note="tetracysteine peptide that binds biarsenical
                     labeling reagents"
     CDS             complement(995..1021)
                     /label="9xHis"
                     /note="9xHis affinity tag"
     CDS             complement(1082..1099)
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             complement(1121..1138)
                     /label="HQ tag"
                     /note="HQHQHQ metal affinity tag"
     CDS             complement(1136..1156)
                     /label="7xHis"
                     /note="6xHis affinity tag"
     CDS             2592..3152
                     /gene="DHFR"
                     /label="Dihydrofolate reductase"
                     /note="Dihydrofolate reductase from Homo sapiens.
                     Accession#: P00374"
     promoter        complement(3776..3793)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3800..3816)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        4290..4394
                     /label="AmpR promoter"
     CDS             4395..5252
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5426..6014
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    6302..6323
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6338..6368
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    6376..6392
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6400..6416
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        6434..6452
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatcctatt tgatgaatta actacactta
       61 aaataataca attattatta aatttttttt tgatttattt attaattttt aaacttaatc
      121 atttgtattt gggaggaatt atatatatct ttataattat tttatttttt tttatttttt
      181 tattttttta ttattattat ttttttttat tttttttttt tactgtatca aagaaaaacc
      241 tttaaaaaaa aaattataat ttccccatct tactatattt ttaatacata cgttttaagg
      301 aattaaatta gacaaaagct atattatgct ttacatataa ttagaattta taaacgtttg
      361 gttattagat atttcatgtc tcagtaaagt ctttcaatac atatgtaaaa aaatatatat
      421 gaatacacat aagttgttaa tatattttat atgcataaat gtataaatat atatatatat
      481 atatatatat gtatgtatgt atatgtgtgt atatgaaatt atttcaatgt ttaatttttt
      541 aaattttaat tttttttttt tttttttttt ttattatgta tattgatctt tattatttaa
      601 atattacttt tttcgttttt tcttcttttt attatttttt ttttttttta tattttatac
      661 aaatggtaat tcaaataaaa ggtataaatt tatatttaat tttcttttat ggataaataa
      721 aagaaaaata taaatatata aaaatataaa aatatatata tgtatattgg ggtgatgata
      781 aaatgaaaga taatatatat atatatatat ctttattttt ttttttttgt agaccccatt
      841 gtgagtacat aaatatatta tataactcga gtcattaatg gtgatggtga tggtgcaagt
      901 cttcttcaga aataagtttt tgttccccgg ggccgccacc gcaacagcca ggacaacagc
      961 caccagcacc ggtacggata tcaggttgta attgatgatg gtggtgatga tggtgatggt
     1021 gtgcttgttt ttgaccatgt acttgttggt gaacctgtgg tgcttggtga tttacttttc
     1081 catgatgatg atggtggtgt gcttggtgtg gagcttggtg ttggtgttga tgttgatggt
     1141 gatggtggtg atggtgttca tgttgctttt gtgctaaagt tctcttattt ctgaaatcga
     1201 aggagtcacc ggatccgttt ccattattgc agttattaga gcacttcaaa acccatacta
     1261 aaaaagagac taaaagaatt ttagtaaaaa cagggaaagc cttctttctc ctcaaagtat
     1321 ttttgttctt aaaacttttc atactagtct cgagggatcc tgatatattt ctattaggta
     1381 tttattatta taaaatataa atcttgaatg ataataaata aaatattagt tattcctttt
     1441 ctagtttaaa atatacatat tataaatata tatatatata tatatatttt tattgtgaca
     1501 agaatatata attataaatt atattattta tttttgtatt tttttttttt tttttttttt
     1561 tttctttttt tgttttattt ttcttttttt ttataaatat tatttttttc ttttatcatg
     1621 cacattggaa taatacatta atatatatat atatattata ttatacatat attgaataat
     1681 gtttataaaa aatgcataac ttatatgaat ataatttttt ttaaatatga caaaaagaaa
     1741 aaaaaaaaaa accaaaaaaa attaaaattg aaatgaaata tataaatata ttatttatat
     1801 atattataca ttgtttaata ctactacatg tatatatata tattatatat atatatatat
     1861 atcaattttt tcaaaaataa attaatataa aaagagggga aaaaaaaaaa aaaaaaaaaa
     1921 aaaagataat taagtaagca tttaaaaata tataaattga taatatataa aattaatcac
     1981 atataagctt gcatgcttag ctaattcgct tgtaagaggt actctcgttt atgcaaaact
     2041 atttgatata gcattttaac aagtacacat atatatatgt aatatatata ctatatatat
     2101 ctattgcatg tgtactaagc atgtgcatgg catccccttt ttctcgtgtt taaaacagtt
     2161 tgtatgataa aatataaagg atttgaaaaa gagaaaaaaa tatatgatct catcctatat
     2221 agcgccataa tttttatttg ggttgaataa aattttctac taaatttagg tgtaagtaaa
     2281 ataatggaat atatataagt acaataaaaa agtgcataaa ttaaaaaatt tttataataa
     2341 atattttttt taaaaaagtc aataataata ttaaatatat ataacacagg attatatatg
     2401 ttcactacaa ttttttatat tataatataa attcttttca attttcattt tattttacat
     2461 acactttcct tttttgtcac tatattttaa tattcacata tttagtttaa atactggcta
     2521 tttctttcta catttgctag taacaattgt gtagtgctta tatatataca cacacctaaa
     2581 acttacaaac catggttggt tcgctaaact gcatcgtcgc tgtgtcccag aacatgggca
     2641 tcggcaagaa cggggacctg ccctggccac cgctcaggaa cgaatttaga tatttccaga
     2701 gaatgaccac aacctcttca gtagaaggta agcagaatct ggtgattatg ggtaagaaga
     2761 cctggttctc cattcctgag aagaatcgac ctttaaaggg tagaattaat ttagttctca
     2821 gcagagaact caaggaacct ccacaaggag ctcattttct ttccagaagt ctagatgatg
     2881 ccttaaaact tactgaacaa ccagaattag caaataaagt agacatggtc tggatagttg
     2941 gtggcagttc tgtttataag gaagccatga atcacccagg ccatcttaaa ctatttgtga
     3001 caaggatcat gcaagacttt gaaagtgaca cgttttttcc agaaattgat ttggagaaat
     3061 ataaacttct gccagaatac ccaggtgttc tctctgatgt ccaggaggag aaaggcatta
     3121 agtacaaatt tgaagtatat gagaagaatg attaaccgcg gggtacccca ttaaatttat
     3181 ttaataatag attaaaaata ttataaaaat aaaaacataa acacagaaat tacaaaaaaa
     3241 atacatatga attttttttt tgtaatcttc cttataaata tagaataatg aatcatataa
     3301 aacatatcat ttttcattta tttacattta aaattattgt ttcagtatct ttaatttatt
     3361 atgtatatat aaaaataact tacaatttta ttaataaaca atatatgttt attaattcat
     3421 gttttgtaat ttatgggata gcgatttttt ttactgtctg tatttttctt ttttaattat
     3481 gttttaattg tattttattt ttattattgt tctttttata gtattatttt aaaacaaaat
     3541 gttttttcta agaacttata ataataataa atataaattt taataaaaat tatatttatc
     3601 ttttacaata tgaacataaa gtacaacatt aatatatagc ttttaatatt tttattccta
     3661 atcatgtaaa tcttaaattt ttctttttaa acatatgtta aatatttatt tctcattata
     3721 tataaggaca tatttattaa accgcagaga aatctagagg taccgagctc gaattctata
     3781 gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     3841 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     3901 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc
     3961 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
     4021 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     4081 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     4141 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     4201 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     4261 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     4321 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     4381 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     4441 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     4501 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     4561 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     4621 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     4681 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     4741 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     4801 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     4861 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     4921 gtgacaccac gatgcctgta gcaatgccaa caacgttgcg caaactatta actggcgaac
     4981 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     5041 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     5101 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     5161 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     5221 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     5281 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     5341 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     5401 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     5461 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     5521 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     5581 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     5641 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     5701 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     5761 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     5821 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     5881 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     5941 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     6001 ggagcctatc gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     6061 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     6121 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     6181 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     6241 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     6301 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     6361 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     6421 attacgccaa gctatttagg tgacactata gaatactc
//
