LOCUS       40924_14140       13621 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pDART, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13621)
  AUTHORS   Ryu J, Lee U, Park J, Yoo DH, Ahn JH.
  TITLE     A Vector System for ABC Transporter-Mediated Secretion and 
            Purification of Recombinant Proteins in Pseudomonas Species
  JOURNAL   Appl. Environ. Microbiol. 81 (5), 1744-1753 (2015)
  PUBMED    25548043
REFERENCE   2  (bases 1 to 13621)
  AUTHORS   Ryu J, Lee U, Park J, Yoo D-H., Ahn JH.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2014) Dept. Biology, Korea Science Academy of 
            KAIST, #899, Tanggam 3-Dong, Busanjin-Gu, Busan 614-822, Korea
REFERENCE   3  (bases 1 to 13621)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13621)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2015"; volume: "81"; issue: "5"; pages:
            "1744-1753"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-NOV-2014) Dept. Biology, Korea Science Academy of KAIST, #899, 
            Tanggam 3-Dong, Busanjin-Gu, Busan 614-822, Korea"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13621
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..17)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1002..1814
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(2631..3025)
                     /direction=LEFT
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            3367..3454
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             4693..5661
                     /label=RSF1010 RepB
                     /note="replication protein B of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     CDS             6175..7011
                     /label=RSF1010 RepA
                     /note="replication protein A of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     CDS             7001..7849
                     /label=RSF1010 RepC
                     /note="replication protein C of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     protein_bind    8516..8537
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8552..8582
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8590..8606
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8614..8630
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             8674..10410
                     /codon_start=1
                     /product="TliD"
                     /label=TliD
                     /note="ABC protein"
                     /protein_id="AJC64569.1"
                     /translation="MSFGSRKINHYGQAYCRGAFIQGVGEYKSILISVGCFTALINLLM
                     LVPSIYMLQVYDRVLSSQNETTLVMLTLMVVGFFAFIGTLEVIRSFIVIRIGSQLERRF
                     NLRVYKAAFERNLQRGQGHAGQALGDLTLLRQFITGPALFAFFDAPWFPLYLLVIFLFN
                     VWLGVLATAGAVLLIGLACLNEYLTKKPLGEAGAYSQQSSQLATSHLHNAETIQAMGML
                     GALRKRWFAVHSQFLGLQNTASDTGSVITSLSKTLRLCLQSLVLGLGALLVIKGDMTAG
                     MMIAGSILMGRVLSPIDQLIAVWKQWSSAKLAYQRLDDLLREFPPDSEPMKLPAPHGQV
                     SFEQVSAGPPGRRTPTLHQVSFTLGAGEVLGVLGASGSGKSTLARVLVGVWPTLGGTVR
                     LDGADIHRWDREDLGPHIGYLPQDIELFSGSIADNIARFRQADPALVVQAAQQAGVHEL
                     ILRLPHGYDTLLGDNGGGLSGGQKQRVALARALYGGPRLIVLDEPNSNLDTVGEAALAS
                     AIVQMKAQGSSVVLVTHRSSALAQADKLLVLNEGRLQRLARARRCCAHCPASRKHRRKG
                     PV"
     CDS             10451..11752
                     /codon_start=1
                     /product="TliE"
                     /label=TliE
                     /note="membrane fusion protein"
                     /protein_id="AJC64570.1"
                     /translation="MSSLTFEQRDARFFVRMGWLLTVVGAGGFFLWASLAPLDQGIPVQ
                     GTVVVSGKRKAVQTFSPGVVSRILVREGETVKQGQPLFRLDQTQNQADVQSLQAQYRLA
                     WASVARWQSERDNRSSIHFPAELSSNPDPALALVLEGQRQLFSSRREAFAREQAGIRAN
                     IDGATAQLGGMRRARSDLTAQAQSLRDQLSNLQPLADNGYIPRNRLMEYQRQLSQVQQD
                     LAQNTGESGRVEQGILESRLKLQQHSEEYQKEVRSQLADAQLRSLTLEQQLTSAGFDLQ
                     HSEINAPADGIAVNLGVHTEGAVVRAGETLLEIVPQGTRLEVEGHLPVHLVDKVGTHLP
                     VDILFTAFNQSRTPRVPGEVSLISADQMLDEKTGAPYYVLRTTLSDAAEQKLQGLVIKP
                     GMPAEMFVRTGERSLLNYLFKPLLDRAGSALTEE"
     CDS             11755..13200
                     /codon_start=1
                     /product="TliF"
                     /label=TliF
                     /note="outer membrane protein"
                     /protein_id="AJC64571.1"
                     /translation="MRSLLIAVLFSCASAHAAMGPFDVYEQALRNDPVFLGAIKERDAG
                     LENRTIGRAGLLPKLSYNYNKGRNNSQATLPDGRGGNYHDDRNYNSYGSTFSLQQPLFD
                     YEAYANYRKGVAQALFADESFRDKSQALLVRVLTYYTQALFAQDQIDIARAKKKAFEQQ
                     FQQNRHLFEQGEGTRTDILEAESRYELATAEEIEALDEQDAALRELGALIGVQSVNIDD
                     LAPLSPGFAAFSLSPANYDTWHELAISNNPTLASQRQALEVARYEVERNRAGHLPKVTA
                     YASSRQQESDSGNTYNQRYDTNTIGVEVSLPLYAGGGVSASTRQASRAMEQAEYELEGK
                     TRETLIELRRQFSACLSGVSKLRAYQKALTSAEALVVSTRQSILGGERVNLDALNAEQQ
                     LYSTRRDLAQARYDYLMAWTKLHYYAGNLRDTDLAKVDEAFGTKRAEPPAADKPPVANK
                     PPEASRPPVASGLAPPWAAQQPQ"
     regulatory      11803..11812
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      13234..13239
                     /regulatory_class="ribosome_binding_site"
     misc_feature    13250..13279
                     /label=multiple cloning site
                     /note="multiple cloning site"
     CDS             13280..13291
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
ORIGIN
        1 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
       61 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
      121 cccttcccaa cagttgcgca gcctgaatgg cgaatgtctg gtcggtgaag tgggctttgg
      181 cgccgccaat atgttctacg atccggctga cagagacgac ctttgtctcg atcctagacg
      241 cattgcacag atggcggacg cattctctcg tgcgctggac gtcgatccgc gtcgcctgct
      301 cgaccaggcg tacgcttatg ggtgcctttc cgcagcttgg aacgcggatg gagaagagga
      361 gcaacgcgat ctagctatcg cggccgcgat caagcaggtg cgacagacgt catactagat
      421 atcaagcgac ttctcctatc ccctgggaac acatcaatct caccggagaa tatcgctggc
      481 caaagcctta gcgtaggatt ccgccccttc ccgcaaacga ccccaaacag gaaacgcagc
      541 tgtaatccgg gcagcgcaac ggaacattca tcagtgtaaa aatggaatca ataaagccct
      601 gcgcagcgcg cagggtcagc ctgaatacgc gtttaatgac cagcacagtc gtgatggcaa
      661 ggtcagaata gcgctgaggt ctgcctcgtg aagaaggtgt tgctgactca taccaggcct
      721 gaatcgcccc atcatccagc cagaaagtga gggagccacg gttgatgaga gctttgttgt
      781 aggtggacca gttggtgatt ttgaactttt gctttgccac ggaacggtct gcgttgtcgg
      841 gaagatgcgt gatctgatcc ttcaactcag caaaagttcg atttattcaa caaagccacg
      901 ttgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa
      961 taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg
     1021 aaacgtcttg ctcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata
     1081 aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgattg tatgggaagc
     1141 ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag
     1201 atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt
     1261 ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccggg aaaacagcat
     1321 tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt
     1381 tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat
     1441 ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg
     1501 atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat aagcttttgc
     1561 cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg
     1621 acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc
     1681 aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc
     1741 tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc
     1801 tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag agcattacgc
     1861 tgacttgacg ggacggcggc tttgttgaat aaatcgaact tttgctgagt tgaaggatca
     1921 gatcacgcat cttcccgaca acgcagaccg ttccgtggca aagcaaaagt tcaaaatcac
     1981 caactggtcc acctacaaca aagctctcat caaccgtggc tccctcactt tctggctgga
     2041 tgatggggcg attcaggcct ggtatgagtc agcaacacct tcttcacgag gcagacctca
     2101 gcgctattct gaccttgcca tcacgactgt gctggtcatt aaacgcgtat tcaggctgac
     2161 cctgcgcgct gcgcagggct ttattgattc catttttaca ctgatgaatg ttccgttgcg
     2221 ctgcccggat tacagctgaa agcgaccagg tgctcggcgt ggcaagactc gcagcgaacc
     2281 cgtagaaagc catgctccag ccgcccgcat tggagaaatt cttcaaattc ccgttgcaca
     2341 tagcccggca attcctttcc ctgctctgcc ataagcgcag cgaatgccgg gtaatactcg
     2401 tcaacgatct gatagagaag ggtttgctcg ggtcggtggc tctggtaacg accagtatcc
     2461 cgatcccggc tggccgtcct ggccgccaca tgaggcatgt tccgcgtcct tgcaatactg
     2521 tgtttacata cagtctatcg cttagcggaa agttctttta ccctcagccg aaatgcctgc
     2581 cgttgctaga cattgccagc cagtgcccgt cactcccgta ctaactgtca cgaacccctg
     2641 caataactgt cacgcccccc tgcaataact gtcacgaacc cctgcaataa ctgtcacgcc
     2701 cccaaacctg caaacccagc aggggcgggg gctggcgggg tgttggaaaa atccatccat
     2761 gattatctaa gaataatcca ctaggcgcgg ttatcagcgc ccttgtgggg cgctgctgcc
     2821 cttgcccaat atgcccggcc agaggccgga tagctggtct attcgctgcg ctaggctaca
     2881 caccgcccca ccgctgcgcg gcagggggaa aggcgggcaa agcccgctaa accccacacc
     2941 aaaccccgca gaaatacgct ggagcgcttt tagccgcttt agcggccttt ccccctaccc
     3001 gaagggtggg ggcgcgtgtg cagccccgca gggcctgtct cggtcgatca ttcagcccgg
     3061 ctcatccttc tggcgtggcg gcagaccgaa caaggcgcgg tcgtggtcgc gttcaaggta
     3121 cgcatccatt gccgccatga gccgatcctc cggccactcg ctgctgttca ccttggccaa
     3181 aatcatggcc cccaccagca ccttgcgcct tgtttcgttc ttgcgctctt gctgctgttc
     3241 ccttgcccgc acccgctgaa tttcggcatt gattcgcgct cgttgttctt cgagcttggc
     3301 cagccgatcc gccgccttgt tgctcccctt aaccatcttg acaccccatt gttaatgtgc
     3361 tgtctcgtag gctatcatgg aggcacagcg gcggcaatcc cgaccctact ttgtagggga
     3421 gggcgcactt accggtttct cttcgagaaa ctggcctaac ggccaccctt cgggcggtgc
     3481 gctctccgag ggccattgca tggagccgaa aagcaaaagc aacagcgagg cagcatggcg
     3541 atttatcacc ttacggcgaa aaccggcagc aggtcgggcg gccaatcggc cagggccaag
     3601 gccgactaca tccagcgcga aggcaagtat gcccgcgaca tggatgaagt cttgcacgcc
     3661 gaatccgggc acatgccgga gttcgtcgag cggcccgccg actactggga tgctgccgac
     3721 ctgtatgaac gcgccaatgg gcggctgttc aaggaggtcg aatttgccct gccggtcgag
     3781 ctgaccctcg accagcagaa ggcgctggcg tccgagttcg cccagcacct gaccggtgcc
     3841 gagcgcctgc cgtatacgct ggccatccat gccggtggcg gcgagaaccc gcactgccac
     3901 ctgatgatct ccgagcggat caatgacggc atcgagcggc ccgccgctca gtggttcaag
     3961 cggtacaacg gcaagacccc ggagaagggc ggggcacaga agaccgaagc gctcaagccc
     4021 aaggcatggc ttgagcagac ccgcgaggca tgggccgacc atgccaaccg ggcattagag
     4081 cgggctggcc acgacgcccg cattgaccac agaacacttg aggcgcaggg catcgagcgc
     4141 ctgcccggtg ttcacctggg gccgaacgtg gtggagatgg aaggccgggg catccgcacc
     4201 gaccgggcag acgtggccct gaacatcgac accgccaacg cccagatcat cgacttacag
     4261 gaataccggg aggcaataga ccatgaacgc aatcgacaga gtgaagaaat ccagaggcat
     4321 caacgagtta gcggagcaga tcgaaccgct ggcccagagc atggcgacac tggccgacga
     4381 agcccggcag gtcatgagcc agacccagca ggccagcgag gcgcaggcgg cggagtggct
     4441 gaaagcccag cgccagacag gggcggcatg ggtggagctg gccaaagagt tgcgggaggt
     4501 agccgccgag gtgagcagcg ccgcgcagag cgcccggagc gcgtcgcggg ggtggcactg
     4561 gaagctatgg ctaaccgtga tgctggcttc catgatgcct acggtggtgc tgctgatcgc
     4621 atcgttgctc ttgctcgacc tgacgccact gacaaccgag gacggctcga tctggctgcg
     4681 cttggtggcc cgatgaagaa cgacaggact ttgcaggcca taggccgaca gctcaaggcc
     4741 atgggctgtg agcgcttcga tatcggcgtc agggacgcca ccaccggcca gatgatgaac
     4801 cgggaatggt cagccgccga agtgctccag aacacgccat ggctcaagcg gatgaatgcc
     4861 cagggcaatg acgtgtatat caggcccgcc gagcaggagc ggcatggtct ggtgctggtg
     4921 gacgacctca gcgagtttga cctggatgac atgaaagccg agggccggga gcctgccctg
     4981 gtagtggaaa ccagcccgaa gaactatcag gcatgggtca aggtggccga cgccgcaggc
     5041 ggtgaacttc gggggcagat tgcccggacg ctggccagcg agtacgacgc cgacccggcc
     5101 agcgccgaca gccgccacta tggccgcttg gcgggcttca ccaaccgcaa ggacaagcac
     5161 accacccgcg ccggttatca gccgtgggtg ctgctgcgtg aatccaaggg caagaccgcc
     5221 accgctggcc cggcgctggt gcagcaggct ggccagcaga tcgagcaggc ccagcggcag
     5281 caggagaagg cccgcaggct ggccagcctc gaactgcccg agcggcagct tagccgccac
     5341 cggcgcacgg cgctggacga gtaccgcagc gagatggccg ggctggtcaa gcgcttcggt
     5401 gatgacctca gcaagtgcga ctttatcgcc gcgcagaagc tggccagccg gggccgcagt
     5461 gccgaggaaa tcggcaaggc catggccgag gccagcccag cgctggcaga gcgcaagccc
     5521 ggccacgaag cggattacat cgagcgcacc gtcagcaagg tcatgggtct gcccagcgtc
     5581 cagcttgcgc gggccgagct ggcacgggca ccggcacccc gccagcgagg catggacagg
     5641 ggcgggccag atttcagcat gtagtgcttg cgttggtact cacgcctgtt atactatgag
     5701 tactcacgca cagaaggggg ttttatggaa tacgaaaaaa gcgcttcagg gtcggtctac
     5761 ctgatcaaaa gtgacaaggg ctattggttg cccggtggct ttggttatac gtcaaacaag
     5821 gccgaggctg gccgcttttc agtcgctgat atggccagcc ttaaccttga cggctgcacc
     5881 ttgtccttgt tccgcgaaga caagcctttc ggccccggca agtttctcgg tgactgatat
     5941 gaaagaccaa aaggacaagc agaccggcga cctgctggcc agccctgacg ctgtacgcca
     6001 agcgcgatat gccgagcgca tgaaggccaa agggatgcgt cagcgcaagt tctggctgac
     6061 cgacgacgaa tacgaggcgc tgcgcgagtg cctggaagaa ctcagagcgg cgcagggcgg
     6121 gggtagtgac cccgccagcg cctaaccacc aactgcctgc aaaggaggca atcaatggct
     6181 acccataagc ctatcaatat tctggaggcg ttcgcagcag cgccgccacc gctggactac
     6241 gttttgccca acatggtggc cggtacggtc ggggcgctgg tgtcgcccgg tggtgccggt
     6301 aaatccatgc tggccctgca actggccgca cagattgcag gcgggccgga tctgctggag
     6361 gtgggcgaac tgcccaccgg cccggtgatc tacctgcccg ccgaagaccc gcccaccgcc
     6421 attcatcacc gcctgcacgc ccttggggcg cacctcagcg ccgaggaacg gcaagccgtg
     6481 gctgacggcc tgctgatcca gccgctgatc ggcagcctgc ccaacatcat ggccccggag
     6541 tggttcgacg gcctcaagcg cgccgccgag ggccgccgcc tgatggtgct ggacacgctg
     6601 cgccggttcc acatcgagga agaaaacgcc agcggcccca tggcccaggt catcggtcgc
     6661 atggaggcca tcgccgccga taccgggtgc tctatcgtgt tcctgcacca tgccagcaag
     6721 ggcgcggcca tgatgggcgc aggcgaccag cagcaggcca gccggggcag ctcggtactg
     6781 gtcgataaca tccgctggca gtcctacctg tcgagcatga ccagcgccga ggccgaggaa
     6841 tggggtgtgg acgacgacca gcgccggttc ttcgtccgct tcggtgtgag caaggccaac
     6901 tatggcgcac cgttcgctga tcggtggttc aggcggcatg acggcggggt gctcaagccc
     6961 gccgtgctgg agaggcagcg caagagcaag ggggtgcccc gtggtgaagc ctaagaacaa
     7021 gcacagcctc agccacgtcc ggcacgaccc ggcgcactgt ctggcccccg gcctgttccg
     7081 tgccctcaag cggggcgagc gcaagcgcag caagctggac gtgacgtatg actacggcga
     7141 cggcaagcgg atcgagttca gcggcccgga gccgctgggc gctgatgatc tgcgcatcct
     7201 gcaagggctg gtggccatgg ctgggcctaa tggcctagtg cttggcccgg aacccaagac
     7261 cgaaggcgga cggcagctcc ggctgttcct ggaacccaag tgggaggccg tcaccgctga
     7321 tgccatggtg gtcaaaggta gctatcgggc gctggcaaag gaaatcgggg cagaggtcga
     7381 tagtggtggg gcgctcaagc acatacagga ctgcatcgag cgcctttgga aggtatccat
     7441 catcgcccag aatggccgca agcggcaggg gtttcggctg ctgtcggagt acgccagcga
     7501 cgaggcggac gggcgcctgt acgtggccct gaaccccttg atcgcgcagg ccgtcatggg
     7561 tggcggccag catgtgcgca tcagcatgga cgaggtgcgg gcgctggaca gcgaaaccgc
     7621 ccgcctgctg caccagcggc tgtgtggctg gatcgacccc ggcaaaaccg gcaaggcttc
     7681 catagatacc ttgtgcggct atgtctggcc gtcagaggcc agtggttcga ccatgcgcaa
     7741 gcgccgccag cgggtgcgcg aggcgttgcc ggagctggtc gcgctgggct ggacggtaac
     7801 cgagttcgcg gcgggcaagt acgacatcac ccggcccaag gcggcaggct gacccccccc
     7861 actctattgt aaacaagaca tttttatctt ttatattcaa tggcttattt tcctgctaat
     7921 tggtaatacc atgaaaaata ccatgctcag aaaaggctta acaatatttt gaaaaattgc
     7981 ctactgagcg ctgccgcaca gctccatagg ccgctttcct ggctttgctt ccagatgtat
     8041 gctcttcttt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     8101 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     8161 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     8221 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     8281 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     8341 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     8401 tcgggaagcg tggcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     8461 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     8521 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     8581 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     8641 ccaagcttgc atgcctgcag gtcgactcta ggggtgtcgt ttggttcaag gaagataaac
     8701 cactatggcc aagcctattg ccgtggcgcc tttattcaag gcgtgggtga atacaagagc
     8761 atcctgatca gtgtcggctg ttttaccgcg ctgattaacc tgttgatgct ggtgccgtcg
     8821 atttacatgc tgcaagtgta tgaccgcgtg ctgtcctcgc agaatgaaac caccctggtc
     8881 atgttgacgc tgatggtcgt ggggttcttt gcgtttattg gcacactgga agttatccgc
     8941 agttttatcg tgatccgtat tggcagccag ttggaacgcc gtttcaactt gcgcgtgtac
     9001 aaggccgcct ttgaacgcaa cctgcaacgc ggccaggggc atgccggcca ggcgctgggc
     9061 gacttgaccc tgttgcgcca gttcatcacc ggcccggcgc tgttcgcgtt tttcgatgcg
     9121 ccgtggtttc ccctctacct gctggtgatt ttcctcttca acgtgtggct cggggtcctg
     9181 gccacggcgg gtgcggtgct gctgatcggc ctggcgtgcc tcaacgaata cctgactaaa
     9241 aagcccttgg gcgaagccgg cgcctattcc cagcaatcga gccaactggc caccagccat
     9301 ttgcacaacg ccgagaccat ccaggccatg ggcatgctcg gtgccctgcg caaacgctgg
     9361 tttgccgtgc attcgcaatt tctggggttg cagaacaccg ccagcgacac cggttcggtg
     9421 atcacgtcct tgagcaaaac cctgcgcctg tgcctgcagt cactggtgtt gggcctgggc
     9481 gcattgctgg tgatcaaggg cgatatgact gccgggatga tgatcgcagg ctccatcctg
     9541 atgggccgcg tattgagccc catcgaccag ttgatcgcgg tgtggaaaca gtggagttcg
     9601 gccaagttgg cctaccagcg cctggacgat ctgctgcgtg aattccctcc cgacagcgag
     9661 ccgatgaaac tcccggcgcc ccacggccag gtgagcttcg agcaggtcag cgcggggccg
     9721 cccgggcggc gcacgccgac cctgcatcag gtcagcttca ccctgggcgc cggcgaagtc
     9781 ctcggcgtgc tcggtgcctc cggttccggc aaatccaccc tggcccgcgt gctggtgggg
     9841 gtgtggccaa ccctgggcgg caccgtgcgc ctggatggcg ccgacatcca tcgctgggac
     9901 cgcgaagacc tcggcccgca cattggctat ctgccccagg acatcgaatt gttcagcggc
     9961 agcatcgccg acaacatcgc gcgttttcgc caggccgatc cggccttggt cgtgcaggct
    10021 gcgcaacagg ccggcgtaca tgagctgatt ctgcggctgc cgcacggcta cgacacgctg
    10081 ctcggcgaca acggcggcgg cctctctggt gggcagaagc agcgggtggc cctggcccgc
    10141 gccctgtatg gcgggccgcg cctgatcgtg ctggatgagc ccaactccaa cctcgacacc
    10201 gtcggcgaag cggccctggc cagcgccatt gtgcagatga aagcccaagg cagcagcgtg
    10261 gtactggtca cccatcgctc ttcggcattg gcccaggccg acaaattgct ggtgctcaac
    10321 gaaggccgcc tgcagcgttt ggcccgagcc aggaggtgtt gcgcgcattg tccggccagc
    10381 cggaagcacc gaaggaaagg cccggtgtga gtttcagtcg tcagtatcaa gcgggaagga
    10441 acccaggcgc atgagcagtc tcacttttga acaacgtgac gcgcggttct tcgtgcgcat
    10501 gggctggttg ctgaccgtgg tcggcgccgg tggatttttc ctctgggcca gcctggcgcc
    10561 gctggaccag ggcattccgg tgcaaggcac cgtcgtggtc tcgggcaagc gcaaagcggt
    10621 gcaaaccttc agcccgggcg tggtcagccg gattctggtg cgcgagggcg aaacggttaa
    10681 acaaggccag ccgctgtttc gcctcgacca gacccagaac caggctgatg tgcagtcgct
    10741 gcaagcccag taccgcctgg cctgggccag cgtggcgcgt tggcagagcg agcgcgacaa
    10801 ccgttccagc atccactttc ccgccgagct gagcagcaac cccgatccgg ccctggccct
    10861 ggtgctggaa ggccagcgcc aactgttcag cagccgccgc gaagcctttg cccgtgagca
    10921 ggcggggatc cgcgcgaaca tcgacggcgc tactgcgcaa ctcggcggca tgcgccgggc
    10981 ccgcagcgac ctgaccgccc aggcccaatc cctgcgtgat caactgagca acctgcagcc
    11041 gctggccgac aatggctaca tcccgcgcaa ccgcctgatg gagtaccagc gccagctgtc
    11101 ccaggtgcag caggacctgg cgcagaacac cggtgaaagc ggccgcgtcg agcagggcat
    11161 tctcgaatcg cgcctgaaac tgcagcagca cagcgaggaa tatcaaaagg aagtgcgcag
    11221 ccaattggcc gacgctcaac tgcgcagcct cacgctggaa cagcaactca cctcggccgg
    11281 gttcgacttg caacacagtg aaatcaacgc gccggccgat ggtattgccg tcaacctcgg
    11341 cgtgcacacc gaaggcgccg tggtgcgcgc cggtgaaacc ctgttggaaa tcgtgcccca
    11401 gggcacgcgc ctggaagtcg aggggcactt gccggtgcac ctggtggaca aggtcggcac
    11461 gcacctgccg gtcgacatcc tgttcaccgc cttcaaccag agccgcacac cacgcgtgcc
    11521 gggggaggtc agcctgatct ccgccgacca gatgctcgac gaaaaaaccg gtgcgccgta
    11581 ttacgtgctg cgcaccacgc tcagcgacgc ggccgagcaa aaactgcagg gcctggtgat
    11641 caagccgggc atgccggccg agatgttcgt gcgcaccggc gagcgctcgc tgctcaatta
    11701 cctgttcaag ccgctgctgg accgcgccgg ctccgcgctg accgaggaat gagcatgaga
    11761 tcgctgctta ttgccgtgtt attcagctgc gccagcgctc atgccgccat gggcccgttc
    11821 gacgtctacg agcaggccct gcgcaacgat ccggtgtttc tcggcgccat caaggagcgc
    11881 gatgccggcc tggagaaccg caccatcggc cgcgccgggc tgctgcccaa gctgtcctac
    11941 aactacaaca agggccgcaa caattcccag gccaccttgc ctgacgggcg cggcggcaat
    12001 tatcacgacg accgcaacta caacagttac ggctcgacct tcagcctgca gcagccgctg
    12061 ttcgactacg aggcctacgc caactaccgc aaaggcgtgg cccaggcgtt gttcgctgat
    12121 gaaagctttc gcgacaagag ccaggcgctg ctggtgcgag tgctgaccta ttacacccag
    12181 gcgctgtttg cccaggacca gatcgacatc gcccgcgcca agaagaaggc cttcgaacag
    12241 cagttccagc agaaccggca cctgttcgag cagggcgagg gcacccgcac cgatattctc
    12301 gaagccgagt cgcgttatga gctggccacg gccgaggaga tcgaggcgct ggacgagcag
    12361 gacgccgccc tgcgcgagct gggggcgttg atcggagtac agagcgtcaa catcgacgac
    12421 ctggcgccgc tgagtcctgg cttcgccgcc ttcagcttga gcccggccaa ctacgacacc
    12481 tggcacgagc tggcgatcag caataacccc acgctggcat cccagcgcca ggccctggaa
    12541 gtggcgcgct atgaagtgga gcgcaaccgc gccgggcacc tgccgaaagt caccgcctat
    12601 gcgagttcgc gccagcagga gtcggacagc ggcaacacct acaaccagcg ctatgacacc
    12661 aacaccatcg gcgtcgaagt cagcctgccg ttgtatgccg gtggcggcgt ctcggcgtcc
    12721 actcgccagg ccagccgcgc catggagcag gccgagtacg agctggaagg caagacgcgc
    12781 gagaccttga tcgaactgcg tcgccaattc agcgcgtgct tgtccggggt gagcaagttg
    12841 cgcgcgtacc agaaggcgct gacgtcggcc gaagcgttgg tggtgtctac ccggcaaagc
    12901 atccttggcg gtgagcgggt caatcttgat gcgttgaatg ccgagcagca gctgtacagc
    12961 acacgccgag acctggccca ggcgcggtat gactacttga tggcctggac caagctgcat
    13021 tactacgccg gcaatttgcg cgacaccgac ctggccaagg tagacgaagc cttcgggacc
    13081 aagagggccg agcctcccgc tgcagacaag ccccctgttg caaacaaacc gcctgaggca
    13141 agccgacccc ctgtggcgag cgggcttgcc ccgccttggg ctgcgcaaca accccaataa
    13201 aaccacacaa tctccggcca attccaacaa caagagagac aagacaatgt ctagacatat
    13261 gggtaccgct agcgagctca ttgaaggacg aggcagcgac ggcaatgacc tgatccaggg
    13321 cggcaagggc gcggacttca tcgaaggcgg caagggcaat gacacgatcc gcgacaacag
    13381 cgggcacaac acctttttgt tcagcgggca ttttggccag gatcggatta tcggctacca
    13441 gccgaccgac aggctggtgt tccagggcgc cgacggcagc accgacctgc gcgaccacgc
    13501 gaaggccgtg ggggccgata cggtgctgag ttttggcgcc gactcggtga ccctggtcgg
    13561 ggtcgggctg ggcggcctgt ggagcgaggg tgtgctgatc agttgaccat agctcgaatt
    13621 c
//
