LOCUS       V008069                 3905 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008069
VERSION     V008069
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Hernday A.
  TITLE     Structure of the Transcriptional Network Controlling White-Opaque
            Switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Hernday A.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-NOV-2012) Microbiology and Immunology, UCSF, 600 16th
            St, San Francisco, CA 94107, USA
REFERENCE   3  (bases 1 to 3905)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3905)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-NOV-2012) Microbiology and Immunology, UCSF, 600 16th St, San
            Francisco, CA 94107, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3905
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    16..32
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             234..263
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     protein_bind    complement(441..460)
                     /label="lac operator (symmetric)"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The
                     symmetric lac operator was optimized for tight binding of
                     lac repressor."
     protein_bind    474..490
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             509..520
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     CDS             527..1180
                     /label="GST"
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     CDS             1211..1906
                     /gene="cpxR"
                     /label="Transcriptional regulatory protein CpxR"
                     /note="Transcriptional regulatory protein CpxR from
                     Escherichia coli (strain K12). Accession#: P0AE88"
     terminator      1923..2009
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(2174..2762)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     terminator      complement(2850..2944)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(2978..3769)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 gacgtctgtg tggaattgtg agcggataac aatttcacac agggccctcg gacaccgagg
       61 agaatgtcaa gaggcgaaca cacaacgtct tggagcgcca gaggaggaac gagctaaaac
      121 ggagcttttt tgccctgcgt gaccagatcc cggagttgga aaacaatgaa aaggccccca
      181 aggtagttat ccttaaaaaa gccacagcat acatcctgtc cgtccaagca gaggagcaaa
      241 agctcatttc tgaagaggac ttgttgcgga aacgacgaga acagttgaaa cacaaacttg
      301 aacagctacg gaactcttgt gcgtaaggaa aagtaaggaa aacgattcct tctaacagaa
      361 atgtcctgag caatcaccta tgaactgtcg actcgagcat agcattttta tccataagat
      421 tagcggatcc taagctttac aattgtgagc gctcacaatt atgatagatt caattgtgag
      481 cggataacaa tttcacacag aattcattaa agaggagaaa ggtaccatgt cccctatact
      541 aggttattgg aaaattaagg gccttgtgca acccactcga cttcttttgg aatatcttga
      601 agaaaaatat gaagagcatt tgtatgagcg cgatgaaggt gataaatggc gaaacaaaaa
      661 gtttgaattg ggtttggagt ttcccaatct tccttattat attgatggtg atgttaaatt
      721 aacacagtct atggccatca tacgttatat agctgacaag cacaacatgt tgggtggttg
      781 tccaaaagag cgtgcagaga tttcaatgct tgaaggagcg gttttggata ttagatacgg
      841 tgtttcgaga attgcatata gtaaagactt tgaaactctc aaagttgatt ttcttagcaa
      901 gctacctgaa atgctgaaaa tgttcgaaga tcgtttatgt cataaaacat atttaaatgg
      961 tgatcatgta acccatcctg acttcatgtt gtatgacgct cttgatgttg ttttatacat
     1021 ggacccaatg tgcctggatg cgttcccaaa attagtttgt tttaaaaaac gtattgaagc
     1081 tatcccacaa attgataagt acttgaaatc cagcaagtat atagcatggc ctttgcaggg
     1141 ctggcaagcc acgtttggtg gtggcgacca tcctccaaaa tcggatctgg ttccgcgtgg
     1201 ccgcggtacc atgaataaaa tcctgttagt tgatgatgac cgagagctga cttccctatt
     1261 aaaggagctg ctcgagatgg aaggcttcaa cgtgattgtt gcccacgatg gggaacaggc
     1321 gcttgatctt ctggacgaca gcattgattt acttttgctt gacgtaatga tgccgaagaa
     1381 aaatggtatc gacacattaa aagcacttcg ccagacacac cagacgcctg tcattatgtt
     1441 gacggcgcgc ggcagtgaac ttgatcgcgt tctcggcctt gagctgggcg cagatgacta
     1501 tctcccgaaa ccgtttaatg atcgtgagct ggtggcacgt attcgcgcga tcctgcgccg
     1561 ttcgcactgg agcgagcaac agcaaaacaa cgacaacggt tcaccgacac tggaagttga
     1621 tgccttagtg ctgaatccag gccgtcagga agccagcttc gacgggcaaa cgctggagtt
     1681 aaccggtact gagtttaccc tgctctattt gctggcacag catctgggtc aggtggtttc
     1741 ccgtgaacat ttaagccagg aagtgttggg caaacgcctg acgcctttcg accgcgctat
     1801 tgatatgcac atttccaacc tgcgtcgtaa actgccggat cgtaaagatg gtcacccgtg
     1861 gtttaaaacc ttgcgtggtc gcggctatct gatggtttct gcttcatgat atctagaggc
     1921 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     1981 cggtgaacgc tctcctgagt aggacaaatc cgccgcccta gacctagggc gttcggctgc
     2041 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     2101 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     2161 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     2221 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     2281 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     2341 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     2401 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     2461 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     2521 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     2581 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     2641 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     2701 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     2761 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     2821 aagggatttt ggtcatgact agtgcttgga ttctcaccaa taaaaaacgc ccggcggcaa
     2881 ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg
     2941 agtccaagcg agctctcgaa ccccagagtc ccgctcagaa gaactcgtca agaaggcgat
     3001 agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag
     3061 cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc
     3121 ggtccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca
     3181 tgatattcgg caagcaggca tcgccatggg tcacgacgag atcctcgccg tcgggcatgc
     3241 gcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat
     3301 catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg
     3361 cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag
     3421 ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca
     3481 cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc
     3541 aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgc agttcattca
     3601 gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga
     3661 acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct
     3721 ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc
     3781 ctcatcctgt ctcttgatca gatcttgatc ccctgcgcca tcagatcctt ggcggcaaga
     3841 aagccatcca gtttactttg cagggcttcc caaccttacc agagggcgcc ccagctggca
     3901 attcc
//
