LOCUS       V017283                 9646 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V017283
VERSION     V017283
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9646)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9646
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(552..1409)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1410..1514)
                     /label="AmpR promoter"
     primer_bind     1988..2004
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        2019..2245
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2246..2426
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2473..2598
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    3091..3324
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             3509..3553
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             3702..3743
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    3820..3936
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        4007..4310
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             5217..5792
                     /label="Rac1 (Q61L)"
                     /note="constitutively active mutant of human Rac family
                     small GTPase 1"
     promoter        5880..6091
                     /label="EF-1-alpha core promoter"
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
     LTR             6104..6372
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     CDS             6406..7080
                     /label="mRFP1"
                     /note="monomeric derivative of DsRed (Campbell et al.,
                     2002)"
     CDS             7138..7734
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    7738..8326
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             8400..8633
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8705..8839
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      8866..9001
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     primer_bind     complement(9034..9050)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(9058..9074)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9082..9112)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(9127..9148)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(join(9436..9646,1..378))
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
       61 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
      121 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
      181 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
      241 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
      301 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
      361 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
      421 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
      481 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
      541 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
      601 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
      661 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
      721 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
      781 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
      841 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
      901 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
      961 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     1021 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     1081 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     1141 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     1201 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     1261 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     1321 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     1381 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     1441 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     1501 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     1561 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
     1621 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     1681 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     1741 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     1801 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc
     1861 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     1921 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     1981 cgacgttgta aaacgacggc cagtgccaag ctgacgcgtg tagtcttatg caatactctt
     2041 gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca
     2101 ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca
     2161 gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt
     2221 taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga
     2281 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     2341 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     2401 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     2461 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     2521 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     2581 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     2641 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     2701 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     2761 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     2821 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     2881 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     2941 actgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     3001 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     3061 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     3121 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     3181 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     3241 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     3301 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     3361 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     3421 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     3481 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     3541 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     3601 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     3661 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     3721 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
     3781 cattcgatta gtgaacggat ctcgacggta tcggttaact tttaaaagaa aaggggggat
     3841 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     3901 agaattacaa aaacaaatta caaaattcaa aattttatcg atagactagt tattaatagt
     3961 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     4021 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     4081 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     4141 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta
     4201 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg
     4261 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     4321 tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     4381 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     4441 gtcgtaatac ccccgccccg ttgacgcaaa tgggcaagct tgccgggtcg aggtaggcgt
     4501 gtacggtggg aggcctatat aagcaaccgg tataatcaaa cagaccagat tgtctgtttg
     4561 ttaccggtgt ttagtgaacc gggcgcgcct catatcgcct ggagacgcca tccacgctgt
     4621 tttgacctcc atagaagaca ccgggaccga tccagcctcc gcggtcactc tcttccgcat
     4681 cgctgtctgc gagggccagc tgttgggctc gcggttgagg acaaactctt cgcggtcttt
     4741 ccagtactct tggatcggaa acccgtcggc ctccgaacgg tactccgcca ccgagggacc
     4801 tgagcgagtc cgcatcgacc ggatcggaaa acctctcgag aaaggcgtct aaccagtcac
     4861 agtcgcaagg taggctgagc accgtggcgg gcggcagcgg gtggcggtcg gggttgtttc
     4921 tggcggaggt gctgctgatg atgtaattaa agtaggcggt cttgagacgg cggatggtcg
     4981 aggtgaggtg tggcaggctt gagatccagc tgttggggtg agtactccct ctcaaaagcg
     5041 ggcattactt ctgcgctaag attgtcagtt tccaaaaacg aggaggattt gatattcacc
     5101 tggcccgatc tggccataca cttgagtgac aatgacatcc actttgcctt tctctccaca
     5161 ggtgtccact cccaggtcca agtttggtct agtcgactgg atccggtacc gaattcatgc
     5221 aggccatcaa gtgtgtggtg gtgggagacg gagctgtagg taaaacttgc ctactgatca
     5281 gttacacaac caatgcattt cctggagaat atatccctac tgtctttgac aattattctg
     5341 ccaatgttat ggtagatgga aaaccggtga atctgggctt atgggataca gctggactag
     5401 aagattatga cagattacgc cccctatcct atccgcaaac agatgtgttc ttaatttgct
     5461 tttcccttgt gagtcctgca tcatttgaaa atgtccgtgc aaagtggtat cctgaggtgc
     5521 ggcaccactg tcccaacact cccatcatcc tagtgggaac taaacttgat cttagggatg
     5581 ataaagacac gatcgagaaa ctgaaggaga agaagctgac tcccatcacc tatccgcagg
     5641 gtctagccat ggctaaggag attggtgctg taaaatacct ggagtgctcg gcgctcacac
     5701 agcgaggcct caagacagtg tttgacgaag cgatccgagc agtcctctgc ccgcctcccg
     5761 tgaagaagag gaagagaaaa tgcctgctgt tgtaactcga gatatctaga gctagcgaat
     5821 tcgaatttaa atcggatccg cggccgcaag gatctgcgat cgctccggtg cccgtcagtg
     5881 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
     5941 gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     6001 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     6061 ttttcgcaac gggtttgccg ccagaacaca gctgaagctt cgaggggctc gcatctctcc
     6121 ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc
     6181 gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag
     6241 gtcgagaccg ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc
     6301 cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg
     6361 ccgttacaga tccaagctgt gaccggcgcc tacgatatcg ccaccatggc cagctccgag
     6421 gatgtcatca aagagtttat gagatttaag gtcaagatgg agggaagcgt caacggacac
     6481 gagttcgaga ttgagggaga aggagaaggc cggccttacg agggcacaca aaccgctaag
     6541 ctcaaggtca caaaaggagg acccctcccc ttctcctggg atattctgag ccctcagttc
     6601 cagtacggaa gcaaagccta tgttaaacac cctgccgaca tccctgacta tctgaagctc
     6661 tccttccctg aaggcttcaa gtgggagaga ttcatgaact tcgaggacgg aggcgtggtg
     6721 acagtcacac aagatagcac cctccaggac ggagagttta tttataaggt gaaactcaga
     6781 ggaaccaact tcccctccga tggccctgtc atgcaaaaaa aaacaatggg atgggaagcc
     6841 tccaccgaga gaatgtatcc tgaggatggc gctctgaaag gcgaaattaa aatgagactg
     6901 aaactcaaag acggaggaca ctacgatgcc gaggtcaaaa caacctacaa ggccaagaaa
     6961 caagtgcagc tgcctggcgc ctacatgact gatattaaac tcgacattat cagccataat
     7021 ggggactaca ccatcgtgga acaatatgag agagctgagg gcagacatag cacaggcgct
     7081 ggagagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctatg
     7141 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     7201 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     7261 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     7321 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     7381 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     7441 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     7501 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     7561 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     7621 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     7681 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat
     7741 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     7801 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     7861 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     7921 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     7981 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     8041 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     8101 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     8161 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     8221 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtctccgc
     8281 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg tacctttaag
     8341 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     8401 ggaagggcta attcactccc aacgaagata agatctgctt tttgcttgta ctgggtctct
     8461 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     8521 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     8581 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     8641 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     8701 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     8761 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     8821 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
     8881 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     8941 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     9001 ctagactttt gcagagacca aattcgtaat catgtcatag ctgtttcctg tgtgaaattg
     9061 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     9121 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     9181 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     9241 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     9301 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     9361 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     9421 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     9481 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     9541 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     9601 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtagg
//
