LOCUS       40924_13185        3082 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Measurement vector pCpxP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Courbet A, Endy D, Renard E, Molina F, Bonnet J.
  TITLE     Detection of pathological biomarkers in human clinical samples via 
            amplifying genetic switches and logic gates
  JOURNAL   Sci Transl Med 7 (289), 289RA83 (2015)
  PUBMED    26019219
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Courbet A, Molina F, Endy D, Bonnet J.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2014) SysDiag CNRS UMR3145, CNRS, 1682 Rue de la 
            Valsiere, Montpellier, Herault 34184, France
REFERENCE   3  (bases 1 to 3082)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3082)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Transl 
            Med"; date: "2015"; volume: "7"; issue: "289"; pages: "289RA83"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-JUL-2014) SysDiag CNRS UMR3145, CNRS, 1682 Rue de la Valsiere, 
            Montpellier, Herault 34184, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..22
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
     regulatory      23..142
                     /label=pCpxP
                     /note="pCpxP"
                     /regulatory_class="promoter"
     misc_RNA        156..206
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     regulatory      228..255
                     /label=JBEI RBS
                     /note="JBEI RBS"
                     /regulatory_class="ribosome_binding_site"
     CDS             256..969
                     /label=superfolder GFP
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Pedelacq et al., 2006)"
     misc_feature    976..996
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      997..1056
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     rep_origin      complement(1307..1895)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(1983..2077)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2101..2757)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(2758..2860)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     terminator      complement(3037..3080)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gaattcgcgg ccgcttctag agaggcagaa attacgtcat cagacgtcgc taatccatga
       61 ctttacgttg ttttacaccc cctgacgcat gtttgcagcc tgaatcgtaa actctctatc
      121 gttgaatcgc gacagaaaga tttgcatgca tgcaagctgt caccggatgt gctttccggt
      181 ctgatgagtc cgtgaggacg aaacagcctc tacaaataat tttgtttaaa gatcttttaa
      241 gaaggagata tacatatgcg taaaggcgaa gagctgttca ctggtgtcgt ccctattctg
      301 gtggaactgg atggtgatgt caacggtcat aagttttccg tgcgtggcga gggtgaaggt
      361 gacgcaacta atggtaaact gacgctgaag ttcatctgta ctactggtaa actgccggta
      421 ccttggccga ctctggtaac gacgctgact tatggtgttc agtgctttgc tcgttatccg
      481 gaccatatga agcagcatga cttcttcaag tccgccatgc cggaaggcta tgtgcaggaa
      541 cgcacgattt cctttaagga tgacggcacg tacaaaacgc gtgcggaagt gaaatttgaa
      601 ggcgataccc tggtaaaccg cattgagctg aaaggcattg actttaaaga agacggcaat
      661 atcctgggcc ataagctgga atacaatttt aacagccaca atgtttacat caccgccgat
      721 aaacaaaaaa atggcattaa agcgaatttt aaaattcgcc acaacgtgga ggatggcagc
      781 gtgcagctgg ctgatcacta ccagcaaaac actccaatcg gtgatggtcc tgttctgctg
      841 ccagacaatc actatctgag cacgcaaagc gttctgtcta aagatccgaa cgagaaacgc
      901 gatcatatgg ttctgctgga gttcgtaacc gcagcgggca tcacgcatgg tatggatgaa
      961 ctgtacaaat gatgatacta gtagcggccg ctgcagtccg gcaaaaaaac gggcaaggtg
     1021 tcaccaccct gccctttttc tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc
     1081 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     1141 aaggcggtaa tcctagctag ggcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     1201 cggtaatacg gttatccaca gaatcagggg nnnacgcagg aaagaacatg tgagcaaaag
     1261 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     1321 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     1381 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     1441 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     1501 aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     1561 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     1621 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     1681 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     1741 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     1801 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     1861 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     1921 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg acttgtgctt
     1981 ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg
     2041 agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta
     2101 cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg
     2161 gaagccatca cagacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc
     2221 ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac
     2281 gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc
     2341 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata
     2401 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc
     2461 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc
     2521 gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat
     2581 aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc
     2641 cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc
     2701 tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt
     2761 agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat
     2821 ttcattatgg tgaaagttgg aacctcttac gtgccgatca acgtctcatt ttcgccagat
     2881 atcgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     2941 aggccctttc gtcttcacct cgagtgccac ctgacgtcta agaaaccatt attatcatga
     3001 cattaaccta taaaaatagg cgtatcacga ggcagaattt cagataaaaa aaatccttag
     3061 ctttcgctaa ggatgatttc tg
//
