LOCUS       40924_13180        3932 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Controller vector pCpxP-Bxb1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Courbet A, Endy D, Renard E, Molina F, Bonnet J.
  TITLE     Detection of pathological biomarkers in human clinical samples via 
            amplifying genetic switches and logic gates
  JOURNAL   Sci Transl Med 7 (289), 289RA83 (2015)
  PUBMED    26019219
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   Courbet A, Molina F, Endy D, Bonnet J.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2014) SysDiag CNRS UMR3145, CNRS, 1682 Rue de la 
            Valsiere, Montpellier, Herault 34184, France
REFERENCE   3  (bases 1 to 3932)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3932)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Transl 
            Med"; date: "2015"; volume: "7"; issue: "289"; pages: "289RA83"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-JUL-2014) SysDiag CNRS UMR3145, CNRS, 1682 Rue de la Valsiere, 
            Montpellier, Herault 34184, France"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..22
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
     regulatory      23..142
                     /label=pCpxP
                     /note="pCpxP"
                     /regulatory_class="promoter"
     misc_RNA        156..206
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     CDS             281..1822
                     /codon_start=1
                     /product="Bxb1 integrase"
                     /label=Bxb1 integrase
                     /protein_id="AKC96463.1"
                     /translation="MRALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDLD
                     VSGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKLV
                     VSATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWGY
                     LPTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQG
                     REPQGREWSATALKRSMISEAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRAE
                     LVKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGTV
                     AMAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRAG
                     SPQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRSM
                     NVRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMSTGAANDENYAANH"
     misc_feature    1826..1846
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      1847..1906
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     rep_origin      complement(2157..2745)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(2833..2927)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2951..3607)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(3608..3710)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     terminator      complement(3887..3930)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gaattcgcgg ccgcttctag agaggcagaa attacgtcat cagacgtcgc taatccatga
       61 ctttacgttg ttttacaccc cctgacgcat gtttgcagcc tgaatcgtaa actctctatc
      121 gttgaatcgc gacagaaaga tttgcatgca tgcaagctgt caccggatgt gctttccggt
      181 ctgatgagtc cgtgaggacg aaacagcctc tacaaataat tttgtttaac ccaagcttgg
      241 gtactagaga agcttgcggc tttcacacct aagagctagc atgagagccc tggtagtcat
      301 ccgcctgtcc cgcgtcaccg atgctacgac ttcaccggag cgtcagctgg agtcttgcca
      361 gcagctctgc gcccagcgcg gctgggacgt cgtcggggta gcggaggatc tggacgtctc
      421 cggggcggtc gatccgttcg accggaagcg cagaccgaac ctggcccggt ggctagcgtt
      481 cgaggagcaa ccgtttgacg tgatcgtggc gtaccgggta gatcggttga cccgatcgat
      541 ccggcatctt cagcagctgg tccactgggc cgaggaccac aagaagctgg tcgtctccgc
      601 gaccgaagcg cacttcgata cgacgacgcc gtttgcggcg gtcgtcatcg cgcttatggg
      661 aacggtggcg cagatggaat tagaagcgat caaagagcgg aaccgttcgg ctgcgcattt
      721 caatatccgc gccgggaaat accgaggatc cctgccgccg tggggatacc tgcctacgcg
      781 cgtggacggg gagtggcggc tggtgccgga ccctgtgcag cgagagcgca tcctcgaggt
      841 gtatcaccgc gtcgtcgaca accacgagcc gctgcatctg gtggcccacg acctgaaccg
      901 gcgtggtgtc ctgtcgccga aggactactt cgcgcagctg caaggccgcg agccgcaggg
      961 ccgggagtgg tcggctaccg cgctgaagcg atcgatgatc tccgaggcga tgctcgggta
     1021 cgcgactctg aacggtaaga ccgtccgaga cgacgacgga gccccgctgg tgcgggctga
     1081 gccgatcctg acccgtgagc agctggaggc gctgcgcgcc gagctcgtga agacctcccg
     1141 ggcgaagccc gcggtgtcta ccccgtcgct gctgctgcgg gtgttgttct gcgcggtgtg
     1201 cggggagccc gcgtacaagt tcgccggggg aggacgtaag cacccgcgct accgctgccg
     1261 ctcgatgggg ttcccgaagc actgcgggaa cggcacggtg gcgatggccg agtgggacgc
     1321 gttctgcgag gagcaggtac tggatctgct cggggacgcg gagcgtctgg agaaagtctg
     1381 ggtagcgggc tcggactccg cggtcgaact cgcggaggtg aacgcggagc tggtggacct
     1441 gacgtcgctg atcggctccc cggcctaccg ggcgggctct ccgcagcgag aagcactgga
     1501 tgcccgtatt gcggcgctgg ccgcgcggca agaggagctg gagggcctgg aggctcgccc
     1561 gtctggctgg gagtggcgcg agaccgggca gcggttcggg gactggtggc gggagcagga
     1621 caccgcggca aagaacacct ggcttcggtc gatgaacgtt cggctgacgt tcgacgtccg
     1681 cggcgggctg actcgcacga tcgacttcgg ggatcttcag gagtacgagc agcatctcag
     1741 gctcggcagc gtggtcgaac ggctacacac cgggatgtcg accggcgctg caaacgacga
     1801 aaactacgct gctaatcatt aataatacta gtagcggccg ctgcagtccg gcaaaaaaac
     1861 gggcaaggtg tcaccaccct gccctttttc tttaaaaccg aaaagattac ttcgcgttat
     1921 gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     1981 agctcactca aaggcggtaa tcctagctag ggcgttcggc tgcggcgagc ggtatcagct
     2041 cactcaaagg cggtaatacg gttatccaca gaatcagggg nnnacgcagg aaagaacatg
     2101 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     2161 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     2221 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     2281 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     2341 gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     2401 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     2461 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     2521 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     2581 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     2641 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     2701 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     2761 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     2821 acttgtgctt ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa
     2881 atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga
     2941 tatcaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc
     3001 tgccgacatg gaagccatca cagacggcat gatgaacctg aatcgccagc ggcatcagca
     3061 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
     3121 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa
     3181 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     3241 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     3301 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca
     3361 ccagctcacc gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa
     3421 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     3481 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     3541 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt
     3601 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta
     3661 gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgccgatca acgtctcatt
     3721 ttcgccagat atcgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     3781 gcgtatcacg aggccctttc gtcttcacct cgagtgccac ctgacgtcta agaaaccatt
     3841 attatcatga cattaaccta taaaaatagg cgtatcacga ggcagaattt cagataaaaa
     3901 aaatccttag ctttcgctaa ggatgatttc tg
//
