LOCUS       V008274                 6340 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008274
VERSION     V008274
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6340)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T,
            Obuchowski M.
  TITLE     Vector system for spore sufrace display
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6340)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T,
            Obuchowski M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2013) Laboratory of Molecular Bacteriology,
            Medical University of Gdansk, Debinki 1, Gdansk 80-211, Poland
REFERENCE   3  (bases 1 to 6340)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6340)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vector NTI Advanced v. 10
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (24-OCT-2013) Laboratory of Molecular Bacteriology, Medical
            University of Gdansk, Debinki 1, Gdansk 80-211, Poland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6340
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             372..1688
                     /gene="lysA"
                     /label="Diaminopimelate decarboxylase"
                     /note="Diaminopimelate decarboxylase from Bacillus subtilis
                     (strain 168). Accession#: P23630"
     misc_recomb     complement(1806..2192)
                     /label="pyrD"
                     /note="pyrD"
     promoter        2714..2818
                     /label="AmpR promoter"
     CDS             2819..3676
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3850..4438
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_recomb     complement(4666..5133)
                     /label="pyrD"
                     /note="pyrD"
     gene            5633..6335
                     /gene="cotG"
                     /label="cotG"
     regulatory      5633..5675
                     /gene="cotG"
                     /regulatory_class="promoter"
     regulatory      5708..5713
                     /gene="cotG"
                     /regulatory_class="ribosome_binding_site"
     CDS             5721..6335
                     /codon_start=1
                     /gene="cotG"
                     /product="CotG"
                     /label="cotG"
                     /note="spore coat protein"
                     /protein_id="AHG54622.1"
                     /translation="MGILELGGGGSHYSHSDIEEAVKSAKKEGLKDYLYQEPHGKKRSH
                     KKSHRTHKKSRSHKKSYCSHKKSRSHKKSFCSHKKSRSHKKSYCSHKKSRSHKKSYRSH
                     KKSRSYKKSYRSYKKSRSYKKSCRSYKKSRSYKKSYCSHKKKSRSYKKSCRTHKKSYRS
                     HKKYYKKPHHHCDDYKRHDDYDSKKEYWKDGNCWVVKKKYK"
     misc_feature    5725..5738
                     /gene="cotG"
                     /label="multiple cloning site"
                     /note="multiple cloning site"
ORIGIN
        1 ccctggattt cactgatgag aatatcgtcg gagataaata taataattcc acggactata
       61 gactatacta gtatactccg tctactgtac gatacacttc cgctcaggtc cttgtccttt
      121 aacgaggatt gttacgtacg ctaatggcgt caaaacaaag actctagatg tggcaggttc
      181 ttgtccgcac agcgaaacct ggcgcaaaag taagacccag catcgttcac cccaaaaatc
      241 gcttaaggca gcctaccaat tcataatcgc attgaaactg actgaagagt atgataatgt
      301 atgtcttaat tatgaaggga cagtccataa aactgtcctt tcatccataa gcggagaaag
      361 agggaatgac attgttctta cacggcacaa gcagacaaaa tcaacatggt catttagaaa
      421 tcggaggtgt ggatgctctc tatttagcgg agaaatatgg tacacctctt tacgtatatg
      481 atgtggcttt aatacgtgag cgtgctaaaa gctttaagca ggcgtttatt tctgcagggc
      541 tgaaagcaca ggtggcatat gcgagcaaag cattctcatc agtcgcaatg attcagctcg
      601 ctgaggaaga gggactttct ttagatgtcg tatccggagg agagctatat acggctgttg
      661 cagcaggctt tccggcagaa cgcatccact ttcatggaaa caataagagc agggaagaac
      721 tgcggatggc gcttgagcac cgcatcggct gcattgtggt ggataatttc tatgaaatcg
      781 cgcttcttga agacctatgt aaagaaacgg gtcactccat cgatgttctt cttcggatca
      841 cgcccggagt agaagcgcat acgcatgact acattacaac gggccaggaa gattcaaagt
      901 ttggtttcga tcttcataac ggacaaactg aacgggccat tgaacaagta ttacaatcgg
      961 aacacattca gctgctgggt gtccattgcc atatcggctc gcaaatcttt gatacggccg
     1021 gttttgtgtt agcagcggaa aaaatcttca aaaaactaga cgaatggaga gattcatatt
     1081 catttgtatc caaggtgctg aatcttggag gaggtttcgg cattcgttat acggaagatg
     1141 atgaaccgct tcatgccact gaatacgttg aaaaaattat cgaagctgtg aaagaaaatg
     1201 cttcccgtta cggttttgac attccggaaa tttggatcga accgggccgt tctctcgtgg
     1261 gagacgcagg cacaactctt tatacggttg gctctcaaaa agaagtgccg ggtgtccgcc
     1321 aatatgtggc tgtagacgga ggcatgaacg acaatattcg tcctgcgctt taccaagcta
     1381 aatatgaagc tgcggcagcc aacaggatcg gagaagcgca tgacaaaacg gtatcaattg
     1441 ccggaaagtg ctgtgaaagc ggagatatgc tgatttggga tattgacctg ccggaagtaa
     1501 aagaaggcga tcttcttgcc gttttttgta caggcgctta tggatacagc atggccaaca
     1561 attataaccg tattccgaga cccgccgttg tatttgtcga aaacggtgag gctcatttag
     1621 tcgtgaagcg agaaacatac gaagatattg taaaacttga tctgccattt aaaacgggtg
     1681 taaagcaata aaagaaacgc cgatttttcg gcgcttttct tatttgaata tagatacaat
     1741 cgcattccag atagatacga agaaatcgca tgcaccattc cttgcggcgg cggtgctcaa
     1801 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg
     1861 acatggatga gcgatgatga tatccgttta ggcgcgccaa aagcgattcc gcctgttttc
     1921 acattcgggc aggaaatatt caattcaaga gcatgaacat taggcgcttt gctgatatgt
     1981 tctgcgactt caacataatc atcgacttga gaacctgcga cattggcaat gatcggtgta
     2041 tcaaactgct caagccacgg cagctcatga tgcaacacac tatccagccc cggattttgg
     2101 agaccgatcg cattgagcat tccagcacca gtctcagcta cccgcggcgt cggattccca
     2161 aagcgcggct cctttgtcgt agccttaatc atgatagctc caagacaaga caaatcataa
     2221 aaacgtgaaa attcttttcc aaaaccgaag cagcctgatg caggaatgat tgggtttttc
     2281 aaatcaagtc ccggcaattt cacctctagc ctggcgtaat agcgaagagg cccgcaccga
     2341 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct
     2401 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
     2461 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     2521 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     2581 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     2641 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     2701 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     2761 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     2821 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     2881 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     2941 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     3001 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     3061 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     3121 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     3181 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     3241 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     3301 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     3361 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     3421 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     3481 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     3541 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     3601 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     3661 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     3721 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     3781 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3841 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3901 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3961 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     4021 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     4081 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     4141 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     4201 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     4261 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     4321 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4381 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4441 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     4501 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     4561 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     4621 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagagcgcg
     4681 atgatgggca ggttgttttt catgattcca gctccttccg atgcattctt caattgattg
     4741 atagccgtat tggagcaaaa cagatgggag ctgttcaata atctctggac atgcaaaagg
     4801 attcacaaag tttgctgttc cgacagcgac tgcgcttgct cccgcgagaa gaaattccag
     4861 ggcatcttca gccgtttgca cgcctcccat tccgataatc gggatgttga ccatctggct
     4921 gacttcatac accatgcgaa tggcaaccgg cttcacagca gggcccgaaa gtccccctgt
     4981 tttattcgct aatatcggtt tgccggtttt taaatcgagt ctcatgccga ttagtgtgtt
     5041 gatcatcgta agaccgtccg ctcccgcttc ctcgatcgct aatgcaattt ctgtgatatt
     5101 agccacgttc ggggcgcgcc aaactggaca catggaaaca cacaaattaa aaactggtct
     5161 gatcgaaata gtacataatg gatttcctta cgcgaaatac gggcagacat ggcctgcccg
     5221 gttattatta tttttgacac cagaccaact ggtaatggta gcgaccggcg ctcaggatcc
     5281 ccgagaaaaa atccagagac aatttgtttc tcatcaagga agggtcttta tactccgcat
     5341 ttaagtgaat ctctcgcgcg ccgcggaatg ttttcggctg ataaaaggaa atatggtatg
     5401 acttcttttt gaagtctctg atatgtgatc cccgataagc gatatcaata tccagccttt
     5461 tttgatttac cttcatcaca gctggcaccg gatcatcgtc ccatatatcc ttttttaatt
     5521 cacgcaagtc ttttggatga acaaacagct gataaagcgg taaattggat tgattcttca
     5581 tccataatcc tccttacaaa ttttaggctt ttatttttat aagatctcag cggaacactt
     5641 atacactttt taaaaccgcg cgtactatga gggtagtaag gatcttcatc cttaacatat
     5701 ttttaaaagg aggatttcaa attggaattc tcgagctcgg tggcggcggt tctcactatt
     5761 cccattctga catcgaagaa gcggtgaaat ccgcaaaaaa agaaggttta aaggattatt
     5821 tataccaaga gcctcatgga aaaaaacgca gtcataaaaa gtcgcaccgc actcacaaaa
     5881 aatctcgcag ccataaaaaa tcatactgct ctcacaaaaa atctcgcagt cacaaaaaat
     5941 cattctgttc tcacaaaaaa tctcgcagcc acaaaaaatc atactgctct cacaagaaat
     6001 ctcgcagcca caaaaaatcg taccgttctc acaaaaaatc tcgcagctat aaaaaatctt
     6061 accgttctta caaaaaatct cgtagctata aaaaatcttg ccgttcttac aaaaaatctc
     6121 gcagctacaa aaagtcttac tgttctcaca agaaaaaatc tcgcagctat aagaagtcat
     6181 gccgcacaca caaaaaatct tatcgttccc ataagaaata ctacaaaaaa ccgcaccacc
     6241 actgcgacga ctacaaaaga cacgatgatt atgacagcaa aaaagaatac tggaaagacg
     6301 gcaattgctg ggtagtcaaa aagaaataca aatagggtac
//
