LOCUS       V008276                 6565 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008276
VERSION     V008276
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6565)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T,
            Obuchowski M.
  TITLE     Vector system for spore sufrace display
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6565)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T,
            Obuchowski M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2013) Laboratory of Molecular Bacteriology,
            Medical University of Gdansk, Debinki 1, Gdansk 80-211, Poland
REFERENCE   3  (bases 1 to 6565)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6565)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vector NTI Advanced v. 10
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (24-OCT-2013) Laboratory of Molecular Bacteriology, Medical
            University of Gdansk, Debinki 1, Gdansk 80-211, Poland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6565
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             378..1694
                     /gene="lysA"
                     /label="Diaminopimelate decarboxylase"
                     /note="Diaminopimelate decarboxylase from Bacillus subtilis
                     (strain 168). Accession#: P23630"
     misc_recomb     complement(1812..2198)
                     /label="pyrD"
                     /note="pyrD"
     promoter        2720..2824
                     /label="AmpR promoter"
     CDS             2825..3682
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3856..4444
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_recomb     complement(4672..5139)
                     /label="pryD"
                     /note="pryD"
     gene            5639..6389
                     /gene="cotG"
                     /label="cotG"
     regulatory      5639..5682
                     /gene="cotG"
                     /regulatory_class="promoter"
     regulatory      5713..5718
                     /gene="cotG"
                     /regulatory_class="ribosome_binding_site"
     CDS             5727..6389
                     /codon_start=1
                     /gene="cotG"
                     /product="CotG"
                     /label="cotG"
                     /note="spore coat protein"
                     /protein_id="AHG54616.1"
                     /translation="MGHYSHSDIEEAVKSAKKEGLKDYLYQEPHGKKRSHKKSHRTHKK
                     SRSHKKSYCSHKKSRSHKKSFCSHKKSRSHKKSYCSHKKSRSHKKSYRSHKKSRSYKKS
                     YRSYKKSRSYKKSCRSYKKSRSYKKSYCSHKKKSRSYKKSCRTHKKSYRSHKKYYKKPH
                     HHCDDYKRHDDYDSKKEYWKDGNCWVVKKKYKGSYGGGGSGGGGSGGGGSSGRACAS"
ORIGIN
        1 cggtacccct ggatttcact gatgagaata tcgtcggaga taaatataat aattccacgg
       61 actatagact atactagtat actccgtcta ctgtacgata cacttccgct caggtccttg
      121 tcctttaacg aggattgtta cgtacgctaa tggcgtcaaa acaaagactc tagatgtggc
      181 aggttcttgt ccgcacagcg aaacctggcg caaaagtaag acccagcatc gttcacccca
      241 aaaatcgctt aaggcagcct accaattcat aatcgcattg aaactgactg aagagtatga
      301 taatgtatgt cttaattatg aagggacagt ccataaaact gtcctttcat ccataagcgg
      361 agaaagaggg aatgacattg ttcttacacg gcacaagcag acaaaatcaa catggtcatt
      421 tagaaatcgg aggtgtggat gctctctatt tagcggagaa atatggtaca cctctttacg
      481 tatatgatgt ggctttaata cgtgagcgtg ctaaaagctt taagcaggcg tttatttctg
      541 cagggctgaa agcacaggtg gcatatgcga gcaaagcatt ctcatcagtc gcaatgattc
      601 agctcgctga ggaagaggga ctttctttag atgtcgtatc cggaggagag ctatatacgg
      661 ctgttgcagc aggctttccg gcagaacgca tccactttca tggaaacaat aagagcaggg
      721 aagaactgcg gatggcgctt gagcaccgca tcggctgcat tgtggtggat aatttctatg
      781 aaatcgcgct tcttgaagac ctatgtaaag aaacgggtca ctccatcgat gttcttcttc
      841 ggatcacgcc cggagtagaa gcgcatacgc atgactacat tacaacgggc caggaagatt
      901 caaagtttgg tttcgatctt cataacggac aaactgaacg ggccattgaa caagtattac
      961 aatcggaaca cattcagctg ctgggtgtcc attgccatat cggctcgcaa atctttgata
     1021 cggccggttt tgtgttagca gcggaaaaaa tcttcaaaaa actagacgaa tggagagatt
     1081 catattcatt tgtatccaag gtgctgaatc ttggaggagg tttcggcatt cgttatacgg
     1141 aagatgatga accgcttcat gccactgaat acgttgaaaa aattatcgaa gctgtgaaag
     1201 aaaatgcttc ccgttacggt tttgacattc cggaaatttg gatcgaaccg ggccgttctc
     1261 tcgtgggaga cgcaggcaca actctttata cggttggctc tcaaaaagaa gtgccgggtg
     1321 tccgccaata tgtggctgta gacggaggca tgaacgacaa tattcgtcct gcgctttacc
     1381 aagctaaata tgaagctgcg gcagccaaca ggatcggaga agcgcatgac aaaacggtat
     1441 caattgccgg aaagtgctgt gaaagcggag atatgctgat ttgggatatt gacctgccgg
     1501 aagtaaaaga aggcgatctt cttgccgttt tttgtacagg cgcttatgga tacagcatgg
     1561 ccaacaatta taaccgtatt ccgagacccg ccgttgtatt tgtcgaaaac ggtgaggctc
     1621 atttagtcgt gaagcgagaa acatacgaag atattgtaaa acttgatctg ccatttaaaa
     1681 cgggtgtaaa gcaataaaag aaacgccgat ttttcggcgc ttttcttatt tgaatataga
     1741 tacaatcgca ttccagatag atacgaagaa atcgcatgca ccattccttg cggcggcggt
     1801 gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga
     1861 gcgtcgacat ggatgagcga tgatgatatc cgtttaggcg cgccaaaagc gattccgcct
     1921 gttttcacat tcgggcagga aatattcaat tcaagagcat gaacattagg cgctttgctg
     1981 atatgttctg cgacttcaac ataatcatcg acttgagaac ctgcgacatt ggcaatgatc
     2041 ggtgtatcaa actgctcaag ccacggcagc tcatgatgca acacactatc cagccccgga
     2101 ttttggagac cgatcgcatt gagcattcca gcaccagtct cagctacccg cggcgtcgga
     2161 ttcccaaagc gcggctcctt tgtcgtagcc ttaatcatga tagctccaag acaagacaaa
     2221 tcataaaaac gtgaaaattc ttttccaaaa ccgaagcagc ctgatgcagg aatgattggg
     2281 tttttcaaat caagtcccgg caatttcacc tctagcctgg cgtaatagcg aagaggcccg
     2341 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta
     2401 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
     2461 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     2521 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     2581 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     2641 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     2701 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     2761 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     2821 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     2881 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     2941 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     3001 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     3061 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     3121 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     3181 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     3241 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     3301 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     3361 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     3421 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     3481 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     3541 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     3601 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     3661 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     3721 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     3781 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     3841 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     3901 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     3961 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     4021 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     4081 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     4141 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     4201 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     4261 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     4321 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     4381 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     4441 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     4501 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     4561 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     4621 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     4681 agcgcgatga tgggcaggtt gtttttcatg attccagctc cttccgatgc attcttcaat
     4741 tgattgatag ccgtattgga gcaaaacaga tgggagctgt tcaataatct ctggacatgc
     4801 aaaaggattc acaaagtttg ctgttccgac agcgactgcg cttgctcccg cgagaagaaa
     4861 ttccagggca tcttcagccg tttgcacgcc tcccattccg ataatcggga tgttgaccat
     4921 ctggctgact tcatacacca tgcgaatggc aaccggcttc acagcagggc ccgaaagtcc
     4981 ccctgtttta ttcgctaata tcggtttgcc ggtttttaaa tcgagtctca tgccgattag
     5041 tgtgttgatc atcgtaagac cgtccgctcc cgcttcctcg atcgctaatg caatttctgt
     5101 gatattagcc acgttcgggg cgcgccaaac tggacacatg gaaacacaca aattaaaaac
     5161 tggtctgatc gaaatagtac ataatggatt tccttacgcg aaatacgggc agacatggcc
     5221 tgcccggtta ttattatttt tgacaccaga ccaactggta atggtagcga ccggcgctca
     5281 ggatcccgag aaaaaatcca gagacaattt gtttctcatc aaggaagggt ctttatactc
     5341 cgcatttaag tgaatctctc gcgcgccgcg gaatgttttc ggctgataaa aggaaatatg
     5401 gtatgacttc tttttgaagt ctctgatatg tgatccccga taagcgatat caatatccag
     5461 ccttttttga tttaccttca tcacagctgg caccggatca tcgtcccata tatccttttt
     5521 taattcacgc aagtcttttg gatgaacaaa cagctgataa agcggtaaat tggattgatt
     5581 cttcatccat aatcctcctt acaaatttta ggcttttatt tttataagat ctcagcggaa
     5641 cacttataca ctttttaaaa ccgcgcgtac tatgagggta gtaaggatct tcatccttaa
     5701 catattttta aaaggaggat ttcaaattgg gccactattc ccattctgac atcgaagaag
     5761 cggtgaaatc cgcaaaaaaa gaaggtttaa aggattattt ataccaagag cctcatggaa
     5821 aaaaacgcag tcataaaaag tcgcaccgca ctcacaaaaa atctcgcagc cataaaaaat
     5881 catactgctc tcacaaaaaa tctcgcagtc acaaaaaatc attctgttct cacaaaaaat
     5941 ctcgcagcca caaaaaatca tactgctctc acaagaaatc tcgcagccac aaaaaatcgt
     6001 accgttctca caaaaaatct cgcagctata aaaaatctta ccgttcttac aaaaaatctc
     6061 gtagctataa aaaatcttgc cgttcttaca aaaaatctcg cagctacaaa aagtcttact
     6121 gttctcacaa gaaaaaatct cgcagctata agaagtcatg ccgcacacac aaaaaatctt
     6181 atcgttccca taagaaatac tacaaaaaac cgcaccacca ctgcgacgac tacaaaagac
     6241 acgatgatta tgacagcaaa aaagaatact ggaaagacgg caattgctgg gtagtcaaaa
     6301 agaaatacaa aggatcctac ggtggcggtg gctctggtgg cggtggctct ggtggcggtg
     6361 gctctagcgg ccgcgcatgc gctagctaat aaggaattct catgtttgac agcttatcat
     6421 cggcaatagt tacccttatt atcaagataa gaaagaaaag gatttttcgc tacgctcaaa
     6481 tcctttaaaa aaacacaaaa gaccacattt tttaatgtgg tctttattct tcaactaaag
     6541 cacccattag ttcaacaaac gagct
//
