LOCUS       V008277                 6340 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008277
VERSION     V008277
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6340)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T,
            Obuchowski M.
  TITLE     Vector system for spore sufrace display
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6340)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T,
            Obuchowski M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2013) Laboratory of Molecular Bacteriology,
            Medical University of Gdansk, Debinki 1, Gdansk 80-211, Poland
REFERENCE   3  (bases 1 to 6340)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6340)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vectro NTI Advanced v. 10
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (24-OCT-2013) Laboratory of Molecular Bacteriology, Medical
            University of Gdansk, Debinki 1, Gdansk 80-211, Poland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6340
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             406..1722
                     /gene="lysA"
                     /label="Diaminopimelate decarboxylase"
                     /note="Diaminopimelate decarboxylase from Bacillus subtilis
                     (strain 168). Accession#: P23630"
     misc_recomb     complement(1840..2226)
                     /label="pyrD"
                     /note="pyrD"
     promoter        2748..2852
                     /label="AmpR promoter"
     CDS             2853..3710
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3884..4472
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_recomb     complement(4700..5167)
                     /label="pyrD"
                     /note="pyrD"
     gene            5667..6340
                     /gene="cotG"
                     /label="cotG"
     regulatory      5667..5710
                     /gene="cotG"
                     /regulatory_class="promoter"
     regulatory      5741..5746
                     /gene="cotG"
                     /regulatory_class="ribosome_binding_site"
     CDS             5755..6340
                     /codon_start=1
                     /gene="cotG"
                     /product="CotG"
                     /label="cotG"
                     /note="spore coat protein"
                     /protein_id="AHG54612.1"
                     /translation="MGHYSHSDIEEAVKSAKKEGLKDYLYQEPHGKKRSHKKSHRTHKK
                     SRSHKKSYCSHKKSRSHKKSFCSHKKSRSHKKSYCSHKKSRSHKKSYRSHKKSRSYKKS
                     YRSYKKSRSYKKSCRSYKKSRSYKKSYCSHKKKSRSYKKSCRTHKKSYRSHKKYYKKPH
                     HHCDDYKRHDDYDSKKEYWKDGNCWVVKKKYK"
ORIGIN
        1 gatcgaagaa gcttatcgaa ttcgagctcg gtacccctgg atttcactga tgagaatatc
       61 gtcggagata aatataataa ttccacggac tatagactat actagtatac tccgtctact
      121 gtacgataca cttccgctca ggtccttgtc ctttaacgag gattgttacg tacgctaatg
      181 gcgtcaaaac aaagactcta gatgtggcag gttcttgtcc gcacagcgaa acctggcgca
      241 aaagtaagac ccagcatcgt tcaccccaaa aatcgcttaa ggcagcctac caattcataa
      301 tcgcattgaa actgactgaa gagtatgata atgtatgtct taattatgaa gggacagtcc
      361 ataaaactgt cctttcatcc ataagcggag aaagagggaa tgacattgtt cttacacggc
      421 acaagcagac aaaatcaaca tggtcattta gaaatcggag gtgtggatgc tctctattta
      481 gcggagaaat atggtacacc tctttacgta tatgatgtgg ctttaatacg tgagcgtgct
      541 aaaagcttta agcaggcgtt tatttctgca gggctgaaag cacaggtggc atatgcgagc
      601 aaagcattct catcagtcgc aatgattcag ctcgctgagg aagagggact ttctttagat
      661 gtcgtatccg gaggagagct atatacggct gttgcagcag gctttccggc agaacgcatc
      721 cactttcatg gaaacaataa gagcagggaa gaactgcgga tggcgcttga gcaccgcatc
      781 ggctgcattg tggtggataa tttctatgaa atcgcgcttc ttgaagacct atgtaaagaa
      841 acgggtcact ccatcgatgt tcttcttcgg atcacgcccg gagtagaagc gcatacgcat
      901 gactacatta caacgggcca ggaagattca aagtttggtt tcgatcttca taacggacaa
      961 actgaacggg ccattgaaca agtattacaa tcggaacaca ttcagctgct gggtgtccat
     1021 tgccatatcg gctcgcaaat ctttgatacg gccggttttg tgttagcagc ggaaaaaatc
     1081 ttcaaaaaac tagacgaatg gagagattca tattcatttg tatccaaggt gctgaatctt
     1141 ggaggaggtt tcggcattcg ttatacggaa gatgatgaac cgcttcatgc cactgaatac
     1201 gttgaaaaaa ttatcgaagc tgtgaaagaa aatgcttccc gttacggttt tgacattccg
     1261 gaaatttgga tcgaaccggg ccgttctctc gtgggagacg caggcacaac tctttatacg
     1321 gttggctctc aaaaagaagt gccgggtgtc cgccaatatg tggctgtaga cggaggcatg
     1381 aacgacaata ttcgtcctgc gctttaccaa gctaaatatg aagctgcggc agccaacagg
     1441 atcggagaag cgcatgacaa aacggtatca attgccggaa agtgctgtga aagcggagat
     1501 atgctgattt gggatattga cctgccggaa gtaaaagaag gcgatcttct tgccgttttt
     1561 tgtacaggcg cttatggata cagcatggcc aacaattata accgtattcc gagacccgcc
     1621 gttgtatttg tcgaaaacgg tgaggctcat ttagtcgtga agcgagaaac atacgaagat
     1681 attgtaaaac ttgatctgcc atttaaaacg ggtgtaaagc aataaaagaa acgccgattt
     1741 ttcggcgctt ttcttatttg aatatagata caatcgcatt ccagatagat acgaagaaat
     1801 cgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct
     1861 tcctaatgca ggagtcgcat aagggagagc gtcgacatgg atgagcgatg atgatatccg
     1921 tttaggcgcg ccaaaagcga ttccgcctgt tttcacattc gggcaggaaa tattcaattc
     1981 aagagcatga acattaggcg ctttgctgat atgttctgcg acttcaacat aatcatcgac
     2041 ttgagaacct gcgacattgg caatgatcgg tgtatcaaac tgctcaagcc acggcagctc
     2101 atgatgcaac acactatcca gccccggatt ttggagaccg atcgcattga gcattccagc
     2161 accagtctca gctacccgcg gcgtcggatt cccaaagcgc ggctcctttg tcgtagcctt
     2221 aatcatgata gctccaagac aagacaaatc ataaaaacgt gaaaattctt ttccaaaacc
     2281 gaagcagcct gatgcaggaa tgattgggtt tttcaaatca agtcccggca atttcacctc
     2341 tagcctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     2401 tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     2461 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     2521 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     2581 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     2641 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     2701 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     2761 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     2821 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     2881 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     2941 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     3001 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     3061 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     3121 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     3181 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     3241 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     3301 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     3361 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     3421 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     3481 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     3541 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     3601 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     3661 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     3721 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     3781 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     3841 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     3901 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     3961 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     4021 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     4081 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     4141 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     4201 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     4261 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     4321 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     4381 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     4441 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     4501 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     4561 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     4621 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     4681 ccgcgcgttg gccgattcat taatgcagag cgcgatgatg ggcaggttgt ttttcatgat
     4741 tccagctcct tccgatgcat tcttcaattg attgatagcc gtattggagc aaaacagatg
     4801 ggagctgttc aataatctct ggacatgcaa aaggattcac aaagtttgct gttccgacag
     4861 cgactgcgct tgctcccgcg agaagaaatt ccagggcatc ttcagccgtt tgcacgcctc
     4921 ccattccgat aatcgggatg ttgaccatct ggctgacttc atacaccatg cgaatggcaa
     4981 ccggcttcac agcagggccc gaaagtcccc ctgttttatt cgctaatatc ggtttgccgg
     5041 tttttaaatc gagtctcatg ccgattagtg tgttgatcat cgtaagaccg tccgctcccg
     5101 cttcctcgat cgctaatgca atttctgtga tattagccac gttcggggcg cgccaaactg
     5161 gacacatgga aacacacaaa ttaaaaactg gtctgatcga aatagtacat aatggatttc
     5221 cttacgcgaa atacgggcag acatggcctg cccggttatt attatttttg acaccagacc
     5281 aactggtaat ggtagcgacc ggcgctcagg atcccgagaa aaaatccaga gacaatttgt
     5341 ttctcatcaa ggaagggtct ttatactccg catttaagtg aatctctcgc gcgccgcgga
     5401 atgttttcgg ctgataaaag gaaatatggt atgacttctt tttgaagtct ctgatatgtg
     5461 atccccgata agcgatatca atatccagcc ttttttgatt taccttcatc acagctggca
     5521 ccggatcatc gtcccatata tcctttttta attcacgcaa gtcttttgga tgaacaaaca
     5581 gctgataaag cggtaaattg gattgattct tcatccataa tcctccttac aaattttagg
     5641 cttttatttt tataagatct cagcggaaca cttatacact ttttaaaacc gcgcgtacta
     5701 tgagggtagt aaggatcttc atccttaaca tatttttaaa aggaggattt caaattgggc
     5761 cactattccc attctgacat cgaagaagcg gtgaaatccg caaaaaaaga aggtttaaag
     5821 gattatttat accaagagcc tcatggaaaa aaacgcagtc ataaaaagtc gcaccgcact
     5881 cacaaaaaat ctcgcagcca taaaaaatca tactgctctc acaaaaaatc tcgcagtcac
     5941 aaaaaatcat tctgttctca caaaaaatct cgcagccaca aaaaatcata ctgctctcac
     6001 aagaaatctc gcagccacaa aaaatcgtac cgttctcaca aaaaatctcg cagctataaa
     6061 aaatcttacc gttcttacaa aaaatctcgt agctataaaa aatcttgccg ttcttacaaa
     6121 aaatctcgca gctacaaaaa gtcttactgt tctcacaaga aaaaatctcg cagctataag
     6181 aagtcatgcc gcacacacaa aaaatcttat cgttcccata agaaatacta caaaaaaccg
     6241 caccaccact gcgacgacta caaaagacac gatgattatg acagcaaaaa agaatactgg
     6301 aaagacggca attgctgggt agtcaaaaag aaatacaaag
//
