LOCUS       V008278                 5480 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008278
VERSION     V008278
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5480)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T,
            Obuchowski M.
  TITLE     Vector system for spore sufrace display
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5480)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T,
            Obuchowski M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2013) Laboratory of Molecular Bacteriology,
            Medical University of Gdansk, Debinki 1, Gdansk 80-211, Poland
REFERENCE   3  (bases 1 to 5480)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5480)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vector NTI Advanced v. 10
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (24-OCT-2013) Laboratory of Molecular Bacteriology, Medical
            University of Gdansk, Debinki 1, Gdansk 80-211, Poland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5480
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_recomb     complement(401..873)
                     /label="C-terminal part of lacA"
                     /note="C-terminal part of lacA"
     gene            986..1283
                     /gene="cotC"
                     /label="cotC"
     regulatory      986..1032
                     /gene="cotC"
                     /regulatory_class="promoter"
     regulatory      1040..1045
                     /gene="cotC"
                     /regulatory_class="ribosome_binding_site"
     CDS             1053..1283
                     /codon_start=1
                     /gene="cotC"
                     /product="CotC"
                     /label="cotC"
                     /note="spore coat protein"
                     /protein_id="AHG54610.1"
                     /translation="MGTRGSGGGGSGYYKKYKEEYYTVKKTYYKKYYEYDKKDYDCDYD
                     KKYDDYDKKYYDHDKKDYDYVVEYKKHKKHY"
     misc_feature    1056..1070
                     /gene="cotC"
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     regulatory      1348..1533
                     /gene="hisD"
                     /label="promoter region"
                     /note="promoter region"
                     /regulatory_class="promoter"
     CDS             1560..2840
                     /gene="hisD"
                     /label="Histidinol dehydrogenase"
                     /note="Histidinol dehydrogenase from Bacillus subtilis
                     (strain 168). Accession#: O34651"
     misc_recomb     complement(2862..3240)
                     /label="N terminal part of lacA"
                     /note="N terminal part of lacA"
     primer_bind     complement(3259..3275)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3283..3299)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3307..3337)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3352..3373)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3661..4249)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4423..5280)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5281..5385)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cctgatcctg cctcgcttgt
      421 acggagacgc cttttccgtg ccgaaccgga aaaactggag agagagacag gtctgtgatc
      481 agaccctcat agaaatcacg ctgaaattga tcctccaaac gcgcgccgat aaaatacgcc
      541 ttgccctgct gatactcatg gcttgtgacc gctggcgtgc gcgcataaaa atcttcttga
      601 tacaccgctt ccactgaagc tgtctttaca tcaatcacgg ttgcataatc cttcatttca
      661 tatatttggc tgcggtagct gacagcgttt cgatccttcg gatacagggt gtccgtttca
      721 agaggctcaa ctccaaatat agcttgaaga tccggatgcc atccgcctgt gtatgttaag
      781 tcatgctcat tcacaacccc gctgatatac gtcatgacta aggtgccgcc gtcagccgta
      841 aacgctttta aacgggaaac ggtgtcctcg ctggagctca ggataaatcg tttgggccga
      901 tgaaaaatcg gctctttatt ttgatttgtt tttgtgtcat ctgtcttttt ctatcatttg
      961 gacagccctt ttttccttct atgattttag tccaagccgc aaaatctact cgccgtataa
     1021 taaagcgtag taaaaataaa ggaggagtat atatgggtac ccggggatcc ggtggcggcg
     1081 gttctggtta ttacaaaaaa tacaaagaag agtattatac ggtcaaaaaa acgtattata
     1141 agaagtatta cgaatatgat aaaaaagatt atgactgtga ttacgacaaa aaatatgatg
     1201 actatgataa aaaatattat gatcacgata aaaaagacta tgattatgtt gtagagtata
     1261 aaaagcataa aaaacactac taaacgccat taacatctcc tcgtttttac tttcccccgg
     1321 ctattgccgg gtcttttttg tttgtgtcta gagaatttga gatattacgt acctttaata
     1381 cattattcat gattttttca taatgaggag aaggatattt gaactaaaaa tatccttact
     1441 gaagttaagc gttgattttc attctttcat ctgctaacat attatcatat tagcgaacta
     1501 aaggattcgg aattttcctt cataagataa gtcgacgttg tggaaggaga gacggcaaaa
     1561 tgaagatcaa aaccatcagc ggagcggaac ggctttctct caagcgttcc atcgatgccg
     1621 gaacggaaga gcaaagaaag acggtccggt ccattattga agatgttaaa gcaaacggtg
     1681 atcaggcggt tcgcagctac acggcgaaat ttgactgcat cgaaatagac agcccgctgg
     1741 tcacaaaaga agaatttgag gaggcataca cctcactgga ttcacgtctt cttcaggtga
     1801 ttaggcaggc aatcgaaaat attagggaat atcacgaaag acagctgcaa tcctcctggt
     1861 tttatcacag aaaggacggc acaatgctgg ggcagaaagt gacggcgctg gattccgccg
     1921 gtgtatatgt gccgggggga acggccgcat atccttcatc tgttttgatg aatgtgattc
     1981 cggcgctcgt agcgggcgtt gaaagaatcg ttctcgtcac gcctccggga aaagacggtc
     2041 tgctgtctcc tggagttttg gttgctgcag ccgagctggg cataaaagac atttacaaaa
     2101 tgggcggcgc tcaggcgatt gcggcgttag catacggaac agaaaccatt gagccggtgg
     2161 ataaaatcac gggtccgggc aacatttatg tcgcgcttgc caaacgagaa gtgttcggcg
     2221 atgtcgatat cgatatgatt gccggaccga gtgaaatcgt cgtgctggct gatgaaacag
     2281 cgattccgag cgaaattgca gccgatctgc tgtcacaggc ggaacacgat aagcttagct
     2341 catgtgtgtt tgtcacagat tccatggcgc tggccgagac ggtttcagcc gaggtgaata
     2401 agcagctgga aaccttgccg cgaagagaaa tagcggaagc ttcagtgaga gattacggtt
     2461 gtatatatgt ggcggaaaca atggtagaag cgattgagac tgtcaataca ctcgcgccag
     2521 agcatttaga aatcataaca cagtctccgg aagctttgct tggcagtatc aaacatgcgg
     2581 gtgcaatttt cttaggcaga tacagccctg agcctgtcgg tgattatttt gccggaccga
     2641 accatgtgct tccgacaaac ggaacagcca gattttcaag tccattaaac gtaactgatt
     2701 ttcaaaagaa atcgagcatt atttcttaca gtcaatcagc ctttgaagaa catgccgaaa
     2761 gcatcgcggc ttttgccaga ctggaagggt tagaagccca tgccagatca attgaagcga
     2821 gagaacggag gatttctaaa tgagaaaagc ggaagcatgc tggcataaat cacagtggca
     2881 atctcccccg tattcgtttg aaatgtgcca cattaacagc gccgggtgat gtccgtatcg
     2941 ttctgctaat aagcggttga tgtgccgtgt tttttctcgg tagactttag atgtgaggca
     3001 gtggttgtgc cttccgccgt gcagctgttt gacgcgggag gcattgacgc gcaaaacttc
     3061 cggataggtt tgcgacagcc aggccggacg ggctccgctc ggcgttgcta atatgacccg
     3121 gccgcctata ctgtgaatcc gctcaaaaat atcatccagc cattcaaatt gatatacgcc
     3181 ctcctccggc tcaagtgcgc tccatgcaaa aatgccgaca gaaaacgtat tcgtatgaga
     3241 aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     3301 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     3361 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     3421 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     3481 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     3541 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     3601 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     3661 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     3721 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     3781 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     3841 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     3901 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     3961 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     4021 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     4081 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     4141 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     4201 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     4261 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     4321 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     4381 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     4441 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     4501 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     4561 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     4621 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     4681 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     4741 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     4801 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     4861 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     4921 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     4981 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     5041 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     5101 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     5161 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     5221 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     5281 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     5341 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     5401 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     5461 tatcacgagg ccctttcgtc
//
