LOCUS       V008280                 5414 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008280
VERSION     V008280
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T,
            Obuchowski M.
  TITLE     Vector system for spore sufrace display
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5414)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T,
            Obuchowski M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2013) Laboratory of Molecular Bacteriology,
            Medical University of Gdansk, Debinki 1, Gdansk 80-211, Poland
REFERENCE   3  (bases 1 to 5414)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5414)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vector NTI Advanced v. 10
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (24-OCT-2013) Laboratory of Molecular Bacteriology, Medical
            University of Gdansk, Debinki 1, Gdansk 80-211, Poland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5414
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_recomb     complement(401..873)
                     /label="C-terminal part of lacA"
                     /note="C-terminal part of lacA"
     gene            896..1286
                     /gene="cotC"
                     /label="cotC"
     regulatory      896..1032
                     /gene="cotC"
                     /regulatory_class="promoter"
     regulatory      1040..1045
                     /gene="cotC"
                     /regulatory_class="ribosome_binding_site"
     misc_feature    1053..1250
                     /gene="cotC"
                     /note="cotC; spore coat protein"
     misc_feature    1266..1286
                     /gene="cotC"
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     CDS             1494..2774
                     /gene="hisD"
                     /label="Histidinol dehydrogenase"
                     /note="Histidinol dehydrogenase from Bacillus subtilis
                     (strain 168). Accession#: O34651"
     misc_recomb     complement(2796..3174)
                     /label="N-terminal part of lacA"
                     /note="N-terminal part of lacA"
     primer_bind     complement(3193..3209)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3217..3233)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3241..3271)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3286..3307)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3595..4183)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4357..5214)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5215..5319)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cctgatcctg cctcgcttgt
      421 acggagacgc cttttccgtg ccgaaccgga aaaactggag agagagacag gtctgtgatc
      481 agaccctcat agaaatcacg ctgaaattga tcctccaaac gcgcgccgat aaaatacgcc
      541 ttgccctgct gatactcatg gcttgtgacc gctggcgtgc gcgcataaaa atcttcttga
      601 tacaccgctt ccactgaagc tgtctttaca tcaatcacgg ttgcataatc cttcatttca
      661 tatatttggc tgcggtagct gacagcgttt cgatccttcg gatacagggt gtccgtttca
      721 agaggctcaa ctccaaatat agcttgaaga tccggatgcc atccgcctgt gtatgttaag
      781 tcatgctcat tcacaacccc gctgatatac gtcatgacta aggtgccgcc gtcagccgta
      841 aacgctttta aacgggaaac ggtgtcctcg ctggagctca ggataaatcg tttgggccga
      901 tgaaaaatcg gctctttatt ttgatttgtt tttgtgtcat ctgtcttttt ctatcatttg
      961 gacagccctt ttttccttct atgattttag tccaagccgc aaaatctact cgccgtataa
     1021 taaagcgtag taaaaataaa ggaggagtat atatgggtta ttacaaaaaa tacaaagaag
     1081 agtattatac ggtcaaaaaa acgtattata agaagtatta cgaatatgat aaaaaagatt
     1141 atgactgtga ttacgacaaa aaatatgatg actatgataa aaaatattat gatcacgata
     1201 aaaaagacta tgattatgtt gtagagtata aaaagcataa aaaacactac ggtggcggcg
     1261 gttctggtac ccggggatcc tctagagaat ttgagatatt acgtaccttt aatacattat
     1321 tcatgatttt ttcataatga ggagaaggat atttgaacta aaaatatcct tactgaagtt
     1381 aagcgttgat tttcattctt tcatctgcta acatattatc atattagcga actaaaggat
     1441 tcggaatttt ccttcataag ataagtcgac gttgtggaag gagagacggc aaaatgaaga
     1501 tcaaaaccat cagcggagcg gaacggcttt ctctcaagcg ttccatcgat gccggaacgg
     1561 aagagcaaag aaagacggtc cggtccatta ttgaagatgt taaagcaaac ggtgatcagg
     1621 cggttcgcag ctacacggcg aaatttgact gcatcgaaat agacagcccg ctggtcacaa
     1681 aagaagaatt tgaggaggca tacacctcac tggattcacg tcttcttcag gtgattaggc
     1741 aggcaatcga aaatattagg gaatatcacg aaagacagct gcaatcctcc tggttttatc
     1801 acagaaagga cggcacaatg ctggggcaga aagtgacggc gctggattcc gccggtgtat
     1861 atgtgccggg gggaacggcc gcatatcctt catctgtttt gatgaatgtg attccggcgc
     1921 tcgtagcggg cgttgaaaga atcgttctcg tcacgcctcc gggaaaagac ggtctgctgt
     1981 ctcctggagt tttggttgct gcagccgagc tgggcataaa agacatttac aaaatgggcg
     2041 gcgctcaggc gattgcggcg ttagcatacg gaacagaaac cattgagccg gtggataaaa
     2101 tcacgggtcc gggcaacatt tatgtcgcgc ttgccaaacg agaagtgttc ggcgatgtcg
     2161 atatcgatat gattgccgga ccgagtgaaa tcgtcgtgct ggctgatgaa acagcgattc
     2221 cgagcgaaat tgcagccgat ctgctgtcac aggcggaaca cgataagctt agctcatgtg
     2281 tgtttgtcac agattccatg gcgctggccg agacggtttc agccgaggtg aataagcagc
     2341 tggaaacctt gccgcgaaga gaaatagcgg aagcttcagt gagagattac ggttgtatat
     2401 atgtggcgga aacaatggta gaagcgattg agactgtcaa tacactcgcg ccagagcatt
     2461 tagaaatcat aacacagtct ccggaagctt tgcttggcag tatcaaacat gcgggtgcaa
     2521 ttttcttagg cagatacagc cctgagcctg tcggtgatta ttttgccgga ccgaaccatg
     2581 tgcttccgac aaacggaaca gccagatttt caagtccatt aaacgtaact gattttcaaa
     2641 agaaatcgag cattatttct tacagtcaat cagcctttga agaacatgcc gaaagcatcg
     2701 cggcttttgc cagactggaa gggttagaag cccatgccag atcaattgaa gcgagagaac
     2761 ggaggatttc taaatgagaa aagcggaagc atgctggcat aaatcacagt ggcaatctcc
     2821 cccgtattcg tttgaaatgt gccacattaa cagcgccggg tgatgtccgt atcgttctgc
     2881 taataagcgg ttgatgtgcc gtgttttttc tcggtagact ttagatgtga ggcagtggtt
     2941 gtgccttccg ccgtgcagct gtttgacgcg ggaggcattg acgcgcaaaa cttccggata
     3001 ggtttgcgac agccaggccg gacgggctcc gctcggcgtt gctaatatga cccggccgcc
     3061 tatactgtga atccgctcaa aaatatcatc cagccattca aattgatata cgccctcctc
     3121 cggctcaagt gcgctccatg caaaaatgcc gacagaaaac gtattcgtat gagaaagctt
     3181 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     3241 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     3301 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     3361 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     3421 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     3481 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     3541 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     3601 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     3661 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     3721 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     3781 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     3841 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     3901 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     3961 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     4021 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     4081 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     4141 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     4201 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     4261 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     4321 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     4381 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     4441 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     4501 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     4561 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     4621 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     4681 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     4741 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     4801 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     4861 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     4921 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     4981 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     5041 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     5101 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     5161 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     5221 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     5281 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     5341 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     5401 gaggcccttt cgtc
//
