LOCUS       V008281                 5399 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008281
VERSION     V008281
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T,
            Obuchowski M.
  TITLE     Vector system for spore sufrace display
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5399)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T,
            Obuchowski M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2013) Laboratory of Molecular Bacteriology,
            Medical University of Gdansk, Debinki 1, Gdansk 80-211, Poland
REFERENCE   3  (bases 1 to 5399)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5399)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vector NTI Advanced v. 10
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (24-OCT-2013) Laboratory of Molecular Bacteriology, Medical
            University of Gdansk, Debinki 1, Gdansk 80-211, Poland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5399
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_recomb     complement(401..875)
                     /label="C-terminal part of lacA"
                     /note="C-terminal part of lacA"
     gene            986..1250
                     /gene="cotC"
                     /label="cotC"
     regulatory      986..1032
                     /gene="cotC"
                     /regulatory_class="promoter"
     regulatory      1040..1045
                     /gene="cotC"
                     /regulatory_class="ribosome_binding_site"
     misc_feature    1053..1250
                     /gene="cotC"
                     /note="cotC; spore coat protein"
     misc_feature    1251..1271
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     regulatory      1267..1450
                     /gene="hisD"
                     /regulatory_class="promoter"
     CDS             1479..2759
                     /gene="hisD"
                     /label="Histidinol dehydrogenase"
                     /note="Histidinol dehydrogenase from Bacillus subtilis
                     (strain 168). Accession#: O34651"
     misc_recomb     complement(2781..3159)
                     /label="N-terminal part of lacA"
                     /note="N-terminal part of lacA"
     primer_bind     complement(3178..3194)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3202..3218)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3226..3256)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3271..3292)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3580..4168)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4342..5199)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5200..5304)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cctgatcctg cctcgcttgt
      421 acggagacgc cttttccgtg ccgaaccgga aaaactggag agagagacag gtctgtgatc
      481 agaccctcat agaaatcacg ctgaaattga tcctccaaac gcgcgccgat aaaatacgcc
      541 ttgccctgct gatactcatg gcttgtgacc gctggcgtgc gcgcataaaa atcttcttga
      601 tacaccgctt ccactgaagc tgtctttaca tcaatcacgg ttgcataatc cttcatttca
      661 tatatttggc tgcggtagct gacagcgttt cgatccttcg gatacagggt gtccgtttca
      721 agaggctcaa ctccaaatat agcttgaaga tccggatgcc atccgcctgt gtatgttaag
      781 tcatgctcat tcacaacccc gctgatatac gtcatgacta aggtgccgcc gtcagccgta
      841 aacgctttta aacgggaaac ggtgtcctcg ctggagctca ggataaatcg tttgggccga
      901 tgaaaaatcg gctctttatt ttgatttgtt tttgtgtcat ctgtcttttt ctatcatttg
      961 gacagccctt ttttccttct atgattttag tccaagccgc aaaatctact cgccgtataa
     1021 taaagcgtag taaaaataaa ggaggagtat atatgggtta ttacaaaaaa tacaaagaag
     1081 agtattatac ggtcaaaaaa acgtattata agaagtatta cgaatatgat aaaaaagatt
     1141 atgactgtga ttacgacaaa aaatatgatg actatgataa aaaatattat gatcacgata
     1201 aaaaagacta tgattatgtt gtagagtata aaaagcataa aaaacactac ggtacccggg
     1261 gatcctctag agaatttgag atattacgta cctttaatac attattcatg attttttcat
     1321 aatgaggaga aggatatttg aactaaaaat atccttactg aagttaagcg ttgattttca
     1381 ttctttcatc tgctaacata ttatcatatt agcgaactaa aggattcgga attttccttc
     1441 ataagataag tcgacgttgt ggaaggagag acggcaaaat gaagatcaaa accatcagcg
     1501 gagcggaacg gctttctctc aagcgttcca tcgatgccgg aacggaagag caaagaaaga
     1561 cggtccggtc cattattgaa gatgttaaag caaacggtga tcaggcggtt cgcagctaca
     1621 cggcgaaatt tgactgcatc gaaatagaca gcccgctggt cacaaaagaa gaatttgagg
     1681 aggcatacac ctcactggat tcacgtcttc ttcaggtgat taggcaggca atcgaaaata
     1741 ttagggaata tcacgaaaga cagctgcaat cctcctggtt ttatcacaga aaggacggca
     1801 caatgctggg gcagaaagtg acggcgctgg attccgccgg tgtatatgtg ccggggggaa
     1861 cggccgcata tccttcatct gttttgatga atgtgattcc ggcgctcgta gcgggcgttg
     1921 aaagaatcgt tctcgtcacg cctccgggaa aagacggtct gctgtctcct ggagttttgg
     1981 ttgctgcagc cgagctgggc ataaaagaca tttacaaaat gggcggcgct caggcgattg
     2041 cggcgttagc atacggaaca gaaaccattg agccggtgga taaaatcacg ggtccgggca
     2101 acatttatgt cgcgcttgcc aaacgagaag tgttcggcga tgtcgatatc gatatgattg
     2161 ccggaccgag tgaaatcgtc gtgctggctg atgaaacagc gattccgagc gaaattgcag
     2221 ccgatctgct gtcacaggcg gaacacgata agcttagctc atgtgtgttt gtcacagatt
     2281 ccatggcgct ggccgagacg gtttcagccg aggtgaataa gcagctggaa accttgccgc
     2341 gaagagaaat agcggaagct tcagtgagag attacggttg tatatatgtg gcggaaacaa
     2401 tggtagaagc gattgagact gtcaatacac tcgcgccaga gcatttagaa atcataacac
     2461 agtctccgga agctttgctt ggcagtatca aacatgcggg tgcaattttc ttaggcagat
     2521 acagccctga gcctgtcggt gattattttg ccggaccgaa ccatgtgctt ccgacaaacg
     2581 gaacagccag attttcaagt ccattaaacg taactgattt tcaaaagaaa tcgagcatta
     2641 tttcttacag tcaatcagcc tttgaagaac atgccgaaag catcgcggct tttgccagac
     2701 tggaagggtt agaagcccat gccagatcaa ttgaagcgag agaacggagg atttctaaat
     2761 gagaaaagcg gaagcatgct ggcataaatc acagtggcaa tctcccccgt attcgtttga
     2821 aatgtgccac attaacagcg ccgggtgatg tccgtatcgt tctgctaata agcggttgat
     2881 gtgccgtgtt ttttctcggt agactttaga tgtgaggcag tggttgtgcc ttccgccgtg
     2941 cagctgtttg acgcgggagg cattgacgcg caaaacttcc ggataggttt gcgacagcca
     3001 ggccggacgg gctccgctcg gcgttgctaa tatgacccgg ccgcctatac tgtgaatccg
     3061 ctcaaaaata tcatccagcc attcaaattg atatacgccc tcctccggct caagtgcgct
     3121 ccatgcaaaa atgccgacag aaaacgtatt cgtatgagaa agcttggcgt aatcatggtc
     3181 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     3241 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     3301 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     3361 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     3421 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     3481 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     3541 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     3601 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     3661 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     3721 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc
     3781 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     3841 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     3901 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     3961 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     4021 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     4081 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     4141 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     4201 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     4261 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     4321 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     4381 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     4441 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     4501 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     4561 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     4621 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     4681 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     4741 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     4801 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     4861 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     4921 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     4981 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     5041 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     5101 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     5161 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     5221 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     5281 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     5341 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtc
//
