LOCUS       V008284                 8257 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008284
VERSION     V008284
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8257)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T,
            Obuchowski M.
  TITLE     Vector system for spore sufrace display
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8257)
  AUTHORS   Iwanicki A, Hinc K, Stasilojc M, Piatek I, Grela A, Lega T,
            Obuchowski M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2013) Laboratory of Molecular Bacteriology,
            Medical University of Gdansk, Debinki 1, Gdansk 80-211, Poland
REFERENCE   3  (bases 1 to 8257)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8257)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vectro NTI Advanced v. 10
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (24-OCT-2013) Laboratory of Molecular Bacteriology, Medical
            University of Gdansk, Debinki 1, Gdansk 80-211, Poland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8257
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        106..210
                     /label="AmpR promoter"
     CDS             211..1068
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1242..1830
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(2016..2156)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(2261..2449)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     CDS             complement(3705..4724)
                     /codon_start=1
                     /gene="amyE"
                     /product="AmyE"
                     /label="amyE"
                     /note="C-terminal part of amyE"
                     /protein_id="AHG54588.1"
                     /translation="MSDDDIRLGWAVIASRSGSTPLFFSRPEGGGNGVRFPGKSQIGDR
                     GSALFEDQAITAVNRFHNVMAGQPEELSNPNGNNQIFMNQRGSHGVVLANAGSSSVSIN
                     TATKLPDGRYDNKAGAGSFQVNDGKLTGTINARSVAVLYPDDIAKAPHVFLENYKTGVT
                     HSFNDQLTITLRADANTTKAVYQINNGPDDRRLRMEINSQSEKEIQFGKTYTIMLKGTN
                     SDGVTRTEKYSFVKRDPASAKTIGYQNPNHWSQVNAYIYKHDGSRVIELTGSWPGKPMT
                     KNADGIYTLTLPADTDTTNAKVIFNNGSAQVPGQNQPGFDYVLNGLYNDSGLSGSLPH"
     gene            complement(3705..4724)
                     /gene="amyE"
                     /label="amyE"
     misc_feature    4825..4959
                     /gene="cotH"
                     /note="cotH; C-terminal part of cotH"
     gene            4825..4959
                     /gene="cotH"
                     /label="cotH"
     gene            5011..5973
                     /gene="cotB"
                     /label="cotB"
     regulatory      5011..5050
                     /gene="cotB"
                     /regulatory_class="promoter"
     regulatory      5074..5081
                     /gene="cotB"
                     /regulatory_class="ribosome_binding_site"
     CDS             5087..5965
                     /codon_start=1
                     /gene="cotB"
                     /product="CotB"
                     /label="cotB"
                     /note="spore coat protein"
                     /protein_id="AHG54591.1"
                     /translation="MSKRRMKTRSNNEISYYNFLHSMKDKIVTVYRGGPESKKGKLTAV
                     KSDYIALQAEKKIIYYQLEHVKSITEDTNNSTTTIETEEMLDADDFHSLIGHLINQSVQ
                     FNQGGPESKKGRLVWLGDDYAALNTNEDGVVYFNIHHIKSISKHEPDLKIEEQTPVGVL
                     EADDLSEVFKSLTHKWVSINRGGPEAIEGILVDNADGHYTIVKNQEVLRIYPFHIKSIS
                     LGPKGSYKKEDQKNEQNQEDNNDKDSNSFISSKSYSSSKSSKRSLKSSDDQSSGSWNSG
                     GGSRIPWARGR"
     misc_feature    5939..5973
                     /gene="cotB"
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     regulatory      6065..6093
                     /gene="thrC"
                     /regulatory_class="promoter"
     CDS             6191..7246
                     /gene="thrC"
                     /label="Threonine synthase"
                     /note="Threonine synthase from Bacillus subtilis (strain
                     168). Accession#: P04990"
     CDS             complement(7610..8143)
                     /codon_start=1
                     /gene="amyE"
                     /product="AmyE"
                     /label="amyE"
                     /note="N terminal part of amyE"
                     /protein_id="AHG54589.1"
                     /translation="MFAKRFKTSLLPLFAGFLLLFHLVLAGPAAASAETANKSNELTAP
                     SIKSGTILHAWNWSFNTLKHNMKDIHDAGYTAIQTSPINQVKEGNQGDKSMSNWYWLYQ
                     PTSYQIGNRYLGTEQEFKEMCAAAEEYGIKVIVDAVINHTTSDYAAISNEVKSIPNWTH
                     GNTQIKNWSDRNST"
     gene            complement(7610..8143)
                     /gene="amyE"
                     /label="amyE"
ORIGIN
        1 aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
       61 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
      121 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
      181 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
      241 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
      301 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
      361 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
      421 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
      481 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
      541 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
      601 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
      661 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
      721 ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa
      781 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
      841 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
      901 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
      961 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     1021 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     1081 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     1141 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     1201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1321 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1381 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1621 ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1861 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1921 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     1981 cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg
     2041 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc
     2101 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     2161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     2221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     2281 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
     2341 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
     2401 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact tgatgcctcc
     2461 gtgtaagggg gaatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct
     2521 cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca
     2581 actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt
     2641 cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg
     2701 gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc
     2761 gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt
     2821 tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg
     2881 ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga
     2941 gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt
     3001 ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa
     3061 tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg
     3121 cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg
     3181 ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa
     3241 gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc
     3301 tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc
     3361 ataatgggga aggccatcca gcctcgcgtc gcgactaaga aaatgccgtc aaatccgctc
     3421 gccatgactt cactaacgat gcctttgaaa atcttcaagt tcttttctac taattcaagg
     3481 cgtgtctcac caggtttttg gtttgctccg gcgcaaatgc agacaatatc agcatccttg
     3541 cagggtatgt ttctctttga tgtctttttg tttgtgaagt atttcacatt tatattgtgc
     3601 aacacttcac aaacttttgc aagagaaaag ttttgtctga tttatgaaca aaaaagaaac
     3661 catcattgat ggtttctttc ggtaagtccc gtctagcctt gccctcaatg gggaagagaa
     3721 ccgcttaagc ccgagtcatt atataaacca tttagcacgt aatcaaagcc aggctgattc
     3781 tgaccgggca cttgggcgct gccattatta aaaatcactt ttgcgttggt tgtatccgtg
     3841 tccgcaggca gcgtcagcgt gtaaattccg tctgcatttt tagtcattgg ttttccaggc
     3901 caagatccgg tcaattcaat tactcggctc ccatcatgtt tatagatata agcatttacc
     3961 tggctccaat gattcggatt ttgatagccg atggttttgg ccgacgctgg atctctttta
     4021 acaaaactgt atttctcggt cctcgttaca ccatcactgt tcgttccttt taacatgatg
     4081 gtgtatgttt tgccaaattg gatctccttt tccgattgtg aattgatctc catccttaaa
     4141 cgcctgtcgt ctggtccatt attgatttga taaacggctt ttgttgtatt cgcatctgca
     4201 cgcaaggtaa tcgtcagttg atcattgaaa gaatgtgtta cacctgtttt gtaattctca
     4261 aggaaaacat gaggcgcttt tgcaatatca tcaggataaa gcacagctac agacctggca
     4321 ttgatcgtgc ctgtcagttt accatcgttc acttgaaatg aacccgctcc agctttattg
     4381 tcatacctgc catcaggcaa ttttgttgcc gtattgatag agacagagga tgaacctgca
     4441 tttgccagca caacgccatg tgagccgcgc tgattcataa atatctggtt gtttccattc
     4501 gggttcgaga gttcctcagg ctgtccagcc atcacattgt gaaatctatt gaccgcagtg
     4561 atagcctgat cttcaaataa agcactcccg cgatcgccta tttggctttt ccccgggaac
     4621 ctcacaccat ttccgcctcc ctcaggtctg gaaaagaaaa gaggcgtact gcctgaacga
     4681 gaagctatca ccgcccagcc taaacggata tcatcatcgc tcatccatgt cgacgctctc
     4741 ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg
     4801 ccgccgcaag gaatggtgca tgcacggatt aggccgtttg tcctcatgga cccgtataaa
     4861 aagaatgata ttgagcgttt tgaccgtgag ccggatgtga tctgcgagta tattaaaaac
     4921 cgttcacaat acctcaaaga tcatttaagt attttatgaa tgcgtgaaaa tgggtattcg
     4981 cggaaaaagc gacaattagg ctattgaatt agttcaacaa ataaatgtga cacgtatata
     5041 tgcagtatgt ttatcatcta tgtataagtg actaggagga atttgaatga gcaagaggag
     5101 aatgaaaacg cgctcaaata atgaaatatc gtattataac tttttgcact caatgaaaga
     5161 taaaattgtt actgtatatc gtggaggtcc ggaatctaaa aaaggaaaat taacagctgt
     5221 aaaatcagat tatatagctt tacaagctga aaaaaaaata atttattatc agttggagca
     5281 tgtgaaaagt attactgagg ataccaataa tagcaccaca acaattgaga ctgaggaaat
     5341 gctcgatgct gatgattttc atagcttaat cggacattta ataaaccaat cagttcaatt
     5401 taaccaaggg ggtccggaat ctaaaaaagg aagattggtc tggctgggag atgattacgc
     5461 tgcgttaaac acaaatgagg atggggtagt gtattttaat atccatcaca tcaaaagtat
     5521 aagtaaacac gagcctgatt tgaaaataga agagcagacg ccagttggag ttttggaagc
     5581 tgatgattta agcgaggttt ttaagagtct gactcataaa tgggtttcaa ttaatcgtgg
     5641 aggtccggaa gccattgagg gtatccttgt agataatgcc gacggccatt atactatagt
     5701 gaaaaatcaa gaggtgcttc gcatctatcc ttttcacata aaaagcatca gcttaggtcc
     5761 aaaagggtcg tacaaaaaag aggatcaaaa aaatgaacaa aaccaggaag acaataatga
     5821 taaggacagc aattcgttca tttcttcaaa atcatatagc tcatcaaaat catctaaacg
     5881 atcactaaaa tcttcagatg atcaatcatc cggatcctgg aattcaggag gaggatcaag
     5941 aattccatgg gcccgcggcc gctagcagat ctactcgagg atcctctaga aattagaaaa
     6001 taaagaatct tgtcatatca atgcgttaag cgtttacatt cgcaaaattc agcctaatga
     6061 tgtcttgtca aaacaggagc gggcttatat aatgtttgta acttatatgt ctgtgttgag
     6121 ttaacaattg cctgcaggtc gttcaagaag tcaaaagcac atatcgtgta gaagggaacg
     6181 gttggagcta atgtggaaag gacttatcca tcaatataaa gaatttttac ctgtaacaga
     6241 tcaaacaccg gcgctaactt tacatgaagg aaacacacct cttattcacc tgccgaagct
     6301 gtctgagcag ctcggaattg agcttcatgt caaaacggaa ggcgtcaatc ctacgggatc
     6361 atttaaagat cgcggaatgg ttatggctgt ggcaaaggca aaagaagaag gcaatgacac
     6421 gattatgtgc gcgtcaacag gtaacacttc cgctgctgcg gcagcatatg cagcccgtgc
     6481 taacatgaaa tgcattgtca tcatcccgaa cggaaaaatt gcatttggaa aactcgctca
     6541 agctgtcatg tacggagccg agattatcgc aattgacgga aactttgacg atgcgcttaa
     6601 aattgtccgt tccatctgtg agaaatcacc gattgccctt gtcaactcag tcaaccctta
     6661 ccgcattgaa ggccaaaaaa ctgctgcctt cgaagtgtgc gaacagctgg gggaagcgcc
     6721 tgatgtttta gcgatcccgg tcggaaatgc tggaaacatc actgcgtact ggaagggctt
     6781 caaggaatat catgagaaaa acggcacagg ccttccgaaa atgcgcggct ttgaagctga
     6841 gggtgcggcg gcaatcgtgc gcaatgaagt gattgaaaat ccggaaacaa tagcgacagc
     6901 cattcgtatc ggaaacccgg caagctggga caaagctgta aaggcagccg aggaatccaa
     6961 tgggaaaatt gacgaagtca ctgatgatga aatccttcac gcatatcagc tgatcgcccg
     7021 tgtagaaggc gtgtttgcag aaccaggttc ttgcgcgtct atcgcaggag tgctgaaaca
     7081 ggtgaaatcc ggagaaattc cgaaaggcag caaggtcgta gctgtgttaa caggaaacgg
     7141 actgaaagat ccgaacacag cggtcgacat ttcagaaatc aagcctgtca cattgccgac
     7201 tgatgaagac agcatccttg aatatgtaaa aggagcggcc cgtgtatgaa cgaagccgac
     7261 atgctgttct cgtacgtctt cccgagcgaa aacggtctgc gctgcgggac gcgcgaattg
     7321 aattatggcc cacaccagtg gcgcggcgac ttccagttca acatcagccg ctacagtcaa
     7381 cagcaactga tggaaaccag ccatcgccat ctgctgcacg cggaagaagg cacatggctg
     7441 aatatcgacg gtttccatat ggggattggt ggcgacgact cctggagccc gtcagtatcg
     7501 gcggaaatcc agctgagcgc cggtcgctac cattaccagt tggtctggtg tcaaaaataa
     7561 taataaccgg gcaggccatg tctgcccgta tttcgcgtaa ggaaatccat tatgtactat
     7621 ttcgatcaga ccagttttta atttgtgtgt ttccatgtgt ccagtttgga atactcttaa
     7681 cctcattgga aatcgcggca taatcactgg tggtatgatt gatgaccgcg tcaacaatga
     7741 cctttatgcc atattcttca gcggctgcac acatttcttt aaattcttgt tcagtaccta
     7801 agtaacggtt gccaatttga tacgatgtcg gctgatacag ccagtaccag ttcgacatgc
     7861 ttttatctcc ttgattccct tcctttactt ggttaatcgg agatgtctga atggctgtat
     7921 atcctgcatc atgaatatcc ttcatattgt gttttaacgt attgaacgac caattccatg
     7981 catgaagaat ggttccgctt ttgatcgacg gtgctgtaag ctcattcgat ttgttcgccg
     8041 tttcagcact cgcagccgcc ggtcctgcca gaaccaaatg aaacagcaat aaaaatccag
     8101 cgaataacgg cagtaaagag gttttgaatc gttttgcaaa cattcttgac actccttatt
     8161 tgattttttg aagacttact tcggagtcaa aaatccctct tacttcattc ttccgcttcc
     8221 tcctttcaaa ccgatgtgaa gactggagaa ttttgtt
//
