LOCUS       V008291                 7082 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008291
VERSION     V008291
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7082)
  AUTHORS   Raja Abd Rahman RNZ., Wong CF, Salleh AB, Basri M.
  TITLE     Expression vector of pCon5
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7082)
  AUTHORS   Raja Abd Rahman RNZ., Wong CF, Salleh AB, Basri M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2011) Department of Microbiology, Universiti Putra
            Malaysia, Faculty of Biotechnology and Biomolecular Sciences, UPM
            Serdang, Selangor 43400, Malaysia
REFERENCE   3  (bases 1 to 7082)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7082)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (27-JAN-2011) Department of Microbiology, Universiti Putra Malaysia,
            Faculty of Biotechnology and Biomolecular Sciences, UPM Serdang,
            Selangor 43400, Malaysia"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7082
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(62..650)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(824..1681)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1682..1786)
                     /label="AmpR promoter"
     promoter        1977..2054
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             2055..3134
                     /label="lacI"
                     /note="lac repressor"
     CDS             complement(3330..4823)
                     /gene="lasB"
                     /label="Elastase"
                     /note="Elastase from Pseudomonas aeruginosa (strain ATCC
                     15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C
                     / PRS 101 / PAO1). Accession#: P14756"
     misc_feature    complement(4869..4897)
                     /label="operator O3 of PT7(A1/O4/O3)"
                     /note="operator O3 of PT7(A1/O4/O3)"
     protein_bind    4879..4895
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    complement(4905..4910)
                     /label="promoter -10 of PT7(A1/O4/O3)"
                     /note="promoter -10 of PT7(A1/O4/O3)"
     protein_bind    complement(4912..4928)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    complement(4928..4933)
                     /label="promoter -33 of PT7(A1/O4/O3)"
                     /note="promoter -33 of PT7(A1/O4/O3)"
     rep_origin      complement(5662..6013)
                     /direction=LEFT
                     /label="pRO1600 oriV"
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the
                     pRO1600 Rep protein for replication (West et al., 1994)"
     CDS             6027..6857
                     /label="pRO1600 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
     primer_bind     7021..7037
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 ctgcaggagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
       61 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      121 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      181 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      241 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      301 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      361 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      421 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      481 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      541 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      601 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      661 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      721 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      781 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      841 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
      901 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
      961 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1021 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1081 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1141 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1201 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1261 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1321 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1381 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1441 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1501 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1561 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1621 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1681 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1741 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1801 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     1861 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     1921 tgcagctccc ggactcgagc ggcgcgcgcg aaggcgaagc ggcatgcatt tacgttgaca
     1981 ccatcgaatg gtgcaaaacc tttcgcggta tggcatgata gcgcccggaa gagagtcaat
     2041 tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct
     2101 cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg
     2161 aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac
     2221 tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc
     2281 cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg
     2341 tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggggcac aatcttctcg
     2401 cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg
     2461 tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca
     2521 tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg
     2581 cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc
     2641 tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac
     2701 gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg
     2761 gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg
     2821 ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga tacgacgata
     2881 ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc
     2941 tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca
     3001 atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa
     3061 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3121 tggaaagcgg gcagtgagcg caacgcaatt aatgtaagtt agcgcgccgc tcgagtcccg
     3181 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     3241 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     3301 ctgagagtgc accatatgaa gcttttttac aacgcgctcg ggcaggtcac gccgacggtg
     3361 ctgaacgccc gggtgacgtc agccgccgag tagttgcggt tctgcgccga gcgaatcacc
     3421 ccgcaggcgc cgctgttgta gttgctggtg gcggtccagt agtagcggtt ggcgtcgacg
     3481 aacacctcga aggccttgcg ggtatcccag cccggcgaat tggccaacag gtagaacgca
     3541 cggttgtaca cgccgctgga gtggtgcacg tcgatgccgt tgtagtactg cgacgcgttg
     3601 tcgatggatc gcccgtcgcg gctgggctgg tccatgtagc gcagcgcacc gctgcccttc
     3661 ttgatgtcgt agccgatcag gaagtcgttc ttgccgcgca tgtagaattc ggctgcctcg
     3721 ccggccatgt cggagaacgc ttcgttcatt ccgcctgatt gcccgcggta gatcagcccg
     3781 gagttctgct cggtaaagcc gtggctgacc tcgtgggccg ccacgtccag cgacaccagc
     3841 ggatagaaca tggtggcgcc gtcgccgaag agcatcgccg tgccgcccca gtaggcgttc
     3901 tccacgctgc gcccgtagtg caccttcatg tacagcttgt gggtcagcgg gctggtgccg
     3961 aaccagtccc ggtacagttt gaacaccacg ccgccgaaga aatgcgcgtc gttcagcggc
     4021 gaataggcgc cgttgacctg cttgtaggtg ttggtcgggc aggcgaagcg gaacggcgtg
     4081 gtcttgctgt cgtcggtgct gccgttcatg tcgacggtga tgacgttgcc gtcgtccatc
     4141 tcgcagcggt cgttgacgat cagcggaccg tagtcgctac cgtaggtgta cttgccgatc
     4201 ttctggttgc cgccggggcc gcccgcctcg gcgtgggcca ggccttccca ctgatcgagc
     4261 acttcgccgg tcttggcgtc gatgacgaaa tgcggccgcg acagtccctc gccgggaatc
     4321 aggtaggaga cgttgtagac cagttgggcg atgttgctct cgcccaggcg gatcaccagt
     4381 tccactttgt cattctcggt cttgcggccc tgggccttca ggctcttggc ctgggccagc
     4441 acctgctcgg cggataccgc cgcggtggtg ctgcccggca ggtcggcagc gatgttggcg
     4501 acgaaatggc cgctgcgctg cgccgccacg ctcttgccgg gacccttgac ttcggtgatg
     4561 gcttcgccga ccacccgtac gccgttgtgg aattgctcgt agcgggtgac ctgcttgccg
     4621 ttgggcaggg tcgtgctgcg gatcgctttc agttcatcgg caccgccagc gccgaccgcg
     4681 gcttgcaagg tgaccgggcc gggcgcgccc tgggcagcct tgctggggag tttggacacg
     4741 tcgatcaggt cggcggcaag agcggccggc gaaacaccca tgatcgcaac gaacaacagg
     4801 tcaagcgtag aaaccttctt cattctgcag gtcgactcta gaggatcccc gggtaccgag
     4861 ctcgaattct gtgtgaaatt gttatccgct cacaattgaa tctaagtatc attgttatcc
     4921 gctcacaagt caacactctt tttgataaat tttcatatgc ggtgtgaaat accgcacaga
     4981 tgcgtaagga gaaaataccg catcaggcgg caatggcaac aacgttgcgc aaactattaa
     5041 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     5101 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     5161 ctggagccgg tgagcgtgga tctcgcggta tcattgcagc actggggcca gatggtaagc
     5221 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     5281 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     5341 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     5401 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     5461 cgtcagaccc caattacacg ccactggctg tgcttgctgg ggtgacggtg gcaacggtgg
     5521 cggccttgct gggctatcgc gttggaaaga aacgagggaa aggggactga taaaccggtc
     5581 ttagcccctc cccttggtgt ccaaccgctc tgtaggcctc tcaggcgccg ctggtgccgc
     5641 tggttggacg ccaagggtga atccgcctcg ataccctgat tactcgcttc ctgcgccctc
     5701 tcaggcggcg ataggggact ggtaaaacgg ggattgccca gacgcctccc ccgccccttc
     5761 aggggcacaa atgcggcccc aacggggcca cgtagtggtg cgttttttgc gtttccaccc
     5821 ttttcttcct tttccctttt aaacctttta ggacgtctac aggccacgta atccgtggcc
     5881 tgtagagttt aaaaagggac ggatttgttg ccattaaggg acggatttgt tgttaagaag
     5941 ggacggattt gttgttgtaa agggacggat ttgttgtatt gtgggacgca gatacagtgt
     6001 ccccttatac acaaggaatg tcgaacgtgg cctcaccccc aatggtttac aaaagcaatg
     6061 ccctggtcga ggccgcgtat cgcctcagtg ttcaggaaca gcggatcgtt ctggcctgta
     6121 ttagccaggt gaagaggagc gagcctgtca ccgatgaagt gatgtattca gtgacggcgg
     6181 aggacatagc gacgatggcg ggtgtcccta tcgaatcttc ctacaaccag ctcaaagaag
     6241 cggccctgcg cctgaaacgg cgggaagtcc ggttaaccca agagcccaat ggcaagggga
     6301 aaagaccgag tgtgatgatt accggctggg tgcaaacaat catctaccgg gagggtgagg
     6361 gccgtgtaga actcaggttc accaaagaca tgctgccgta cctgacggaa ctcaccaaac
     6421 agttcaccaa atacgccttg gctgacgtgg ccaagatgga cagcacccac gcgatcaggc
     6481 tttacgagct gctcatgcaa tgggacagca tcggccagcg cgaaatagaa attgaccagc
     6541 tgcgaaagtg gtttcaactg gaaggccggt atccctcgat caaggacttc aagttgcgag
     6601 tgcttgatcc agccgtgacg cagatcaacg agcacagccc gctacaggtg gagtgggcgc
     6661 agcgaaagac cgggcgcaag gtcacacatc tgttgttcag ttttggaccg aagaagcccg
     6721 ccaaggcggt gggtaaggcc ccagcgaagc gcaaggccgg gaagatttca gatgctgaga
     6781 tcgcgaaaca ggctcgccct ggtgagacat gggaagcggc ccgcgctcga ctaacccaga
     6841 tgccgctgga tctggcctag aggccgtggc caccacggcc cggcctgcct ttcaggctgc
     6901 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     6961 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     7021 gtaaaacgac ggccagtgaa ttcgagctcg gtacccgggg atcctctaga gtcgacacat
     7081 gt
//
